BrainMap

Mrs. Zorita Diaconeasa

PhD

Lecturer - UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Researcher | Teaching staff

Curriculum Vitae (14/09/2020)

Expertise & keywords

Multifunctional nanolipid-microbubble complexes for an optimized targeted delivery of antitumor agents via ultrasonography Call name: P 4 - Proiecte de cercetare exploratorie - PCE-2021
PN-III-P4-PCE-2021-0378 2022 - 2024

Role in this project: Key expert

Coordinating institution: PROPLANTA SRL

Project partners: PROPLANTA SRL (RO)

Affiliation:

Project website: https://biodiatech.ro/portfolio/mtargeson/

Abstract:

The project mTARGESON proposes new exploratory directions and involves multidisciplinary knowledge in theranostics, based on current challenges and opportunities in oncology using nanoparticles for tumor targeting. Project strategy focuses on innovative solutions: building new and improved carriers (liposome and lipid nanospheres linked to microbubbles) of anticancer agents (Doxorubicin and triterpenoids), targeting tumors by ultrasonography. Such complexes will be tested in vitro as tumor inhibitors and immunostimulants using liver and blood cell lines as well in vivo on immunocompetent animals. Seven objectives are organized in 4 work packages and will be implemented for 32 months. The expertise and experience of the team members in different areas are complementary and very useful for the integrated approach: expertise on selecting anticarcinogens to be inserted in liposomes and lipid nanospheres, in vitro uptake/effects of complexes, use of ultrasonography to deliver these complexes to Walker tumors. The host institution for this project is a center of Biomedical research, with advanced infrastructure and a previous portfolio of international/ national projects. The director is an outstanding researcher with international prestige, the team includes other 3 experienced, employed postdocs and 2 no-nominated researchers. The result indicators include reports, 3 publications in ISI-Q1-Q2 journals, participation in international conferences, training of PhD students.

Innovative solid-state fermentation system for enhancing the nutraceutical content of agrifood by-products Call name: P 4 - Proiecte de cercetare exploratorie - PCE-2021
PN-III-P4-PCE-2021-0750 2022 - 2024

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation:

Project website: https://pce0750.wixsite.com/website

Abstract:

Nutraceuticals (NLs) are biomolecules (carbohydrate derivatives, polyphenolics and fatty acids/structural lipids, carotenoids) that have health-promoting, disease-preventing, or medicinal properties. NLs are found in small amounts in dietary sources, thus it is necessary to develop procedures to increase their concentration and extractability. The agri-food by-products could be appropriate for using them as raw materials in the production of these industrially relevant bioactive compounds under solid-state fermentation (SSF) conditions. The aim of the present project is to develop, for the first time in the field, improved SSF processes using encapsulated fungal spores (individual and mixed-cultures), with different functions/ properties/ biosynthesis mechanisms to enrich simultaneously some abundant agro-industrial by-products (brewer's spent grain and grape pomaces) with valuable hydrophilic (polyphenolics) and lipophilic (polyunsaturated fatty acids and β-carotene) biomolecules.

Iron oxide nanoparticles transported by probiotics - cytotoxicity, bioavailability and their influence on intestinal microflora Call name: P 4 - Proiecte de Cercetare Exploratorie, 2020
PN-III-P4-ID-PCE-2020-2126 2021 - 2023

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation:

Project website: https://pronanoiron.usamvcluj.ro/

Abstract:

Anemia is a global issue affecting 2 billion people. Over 52% of pregnant women suffer from anemia. Scientists' bigger concern is iron bioavailability and gastrointestinal side effects. A promising approach for anemia treatment is the utilization of iron oxide nanoparticles. We will empower the iron oxide nanoparticles with probiotics from Lactobacillus and Bifidobacterium species, leading to a reduction of gastrointestinal discomfort, an increase in iron absorption and reduced problems pose of toxicity.

Our overall objective is to design, develop and test an innovative probiotic (Lactobacillus and Bifidobacteria) carrier iron oxide nanoparticles suitable for i) anemia treatment with ii) minimum side effects (no cytotoxicity) that sustain iii) optimum iron absorption mechanism and iv) ensure the gut microbiota health, in order to maximize their impact. Specific objectives will be achieved through seven work packages (WP1-7):

WP1-Synthesis and characterization

WP2-Functionalization

WP3 -In vitro simulated gastrointestinal interventions

WP4-In vitro cellular uptake and cytotoxicity

WP5-In vivo validation of the anemia treatment using probiotic iron oxide nanoparticles

WP6-Scientific and social innovation, dissemination, communication & exploitation

WP7-Management

The potential impact on budgets for health care is considerable; considering that over 30% of the population is anemic, many due to iron deficiency. The research will encourage an interdisciplinary approach.

Development of novel nanocarriers for enhanced physico-chemical properties, bioactivities and stability of essential oils Call name: P 4 - Proiecte de Cercetare Exploratorie, 2020
PN-III-P4-ID-PCE-2020-1847 2021 - 2023

Role in this project:

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation:

Project website: https://soniasocaci.wixsite.com/nanoseo

Abstract:

Essential oils (EOs) are aromatic volatile oily liquids extracted from different parts of plants, known to possess various bioactivities, including antioxidant, antimicrobial and antiproliferative activities. Despite their numerous proved health benefits, EOs are very sensitive to environmental factors (light, heat, moisture, oxygen) when used as such, having a high volatility, strong odour and poor water solubility. These drawbacks limit their application as active compounds in food, pharmaceuticals or cosmetics. To overcome these shortcomings, the nano-sized delivery systems are considered as one of the most promising technologies. Nanocarriers can be used for entrapment of EOs to uphold their original characteristics during processing and to improve their physico-chemical and functional properties. Thus, the present proposal is focused on the development and comprehensive characterization of three innovative nanoformulations: (i) EOs nanoemulsions, (ii) EOs loaded in chitosan nanoparticles and (iii) EOs-metallic nanoparticles colloidal dispersions. The developed nanoformulations will provide the protection of the EOs bioactive compounds, enhancement of their stability, bioavailability, controlled release and hence in their effectiveness as antioxidants, antimicrobials or antiproliferative agents.

Eco-sustainable bio-conversion of brewers spent grain towards complete valorization Call name: P 4 - Proiecte de Cercetare Exploratorie, 2020
PN-III-P4-ID-PCE-2020-2306 2021 - 2023

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation:

Project website: https://ancafarcas.wixsite.com/sustainable-pce

Abstract:

Brewers spent grain (BSG) is the insoluble residue generated from the production of wort in the brewing industry, being estimated that worldwide the annual output is around 30 million tons of wet spent grain. This plant-derived by-product is known to contain significant amounts of valuable components, which remain unexploited in the brewing processes. Despite the continuous progress from the recent years, in this area there is still a priority to develop/adapt eco-sustainable valorizations procedures because many of the methods proposed to extract individual components are financially expensive, it degrades the environment through high solvent consumption, and by default they have a low feasibility degree. The project entitled: "Eco-sustainable bio-conversion of brewers spent grain towards complete valorization", is focused on developing a procedure that will allow a complete exploitation of BSG as source of bioactive compounds and functional fractions. The first direction was designed to find the most appropriate treatment in order to increase the release and bioavailability of bioactive molecules from lignocellulosic matrix (O1-O2). The second direction aims to optimize the protocol for developing functional fractions and to demonstrate the procedure eco-sustainability. A complex statistical analysis with intelligent chemometric tools will be applied in order to achieve the complete bioconversion system (O3-O5).

Innovative hydrogels containing polymeric micelles as drug delivery systems for oregano essential oil: an advanced pharmaceutical approach for the management of skin tags Call name: P 1 - SP 1.1 - Proiecte de cercetare pentru stimularea tinerelor echipe independente
PN-III-P1-1.1-TE-2019-0130 2020 - 2023

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE " VICTOR BABEŞ " TIMISOARA

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE " VICTOR BABEŞ " TIMISOARA (RO)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE " VICTOR BABEŞ " TIMISOARA (RO)

Project website: http://old.umft.ro/oeopms_907

Abstract:

The experience gained during over ten years of research in the field of natural compounds lead to the identification of a promising natural candidate for a skin problem with which, according to statistics, about half of the population is facing. This problem refers to skin tags (acrochordons), common fibroepithelial benign lesions, with a high rate of prevalence in the adults public, and strongly associated with obesity, insulin resistance and metabolic syndrome. Acrochordons are currently eliminated most commonly by electrocautery, surgical excision or freezing. However among dermatologists it is also recognized that oregano essential oil (OEO) can, in a non-invasive manner eliminate skin tags. Up to date there are no scientific proofs regarding the mechanism of action neither a well established carrier designed in order to eliminate the possible caustic effects (it is generally accepted that essential oils should not be applied directly on the skin ). The aim of this project is to provide and characterize an advanced pharmaceutical formulation for the incorporation of OEO type innovative hydrogels containing polymeric micelles as drug delivery systems (OEO-PMs) as well as to characterize the vitro, in ovo and in vivo effects. The screening will be forwarded towards the assessment of the effect of OEO-PMs hydrogel on the main components of skin tags (keratinocytes, blood vessel- angiogenesis) and also towards the immunomodulatory potential. Modifications in skin characteristics after application (transdermal water loss, erythema, melanin, histological changes) will be analyzed as well as lack of adverse reactions. A success in these directions will lead to both an innovative research line as well as to the possibility of transfer of knowledge to the industry and last but not least to solving the problem of skin tags in a well documented non-invasive manner.

In vitro absorption and bioaccesibility of anthocyanins, their metabolites and precursors Call name: P 1 - SP 1.1 - Proiecte de cercetare pentru stimularea tinerelor echipe independente
PN-III-P1-1.1-TE-2019-0960 2021 - 2023

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: https://sites.google.com/view/anthobioskinte178

Abstract:

Anthocyanins, natural plants pigments found in very significant amount in edible berries and many vegetables, showed to have high antioxidant activity. Because of this they are featured as primary ingredients in food products, natural supplements and cosmetic industry. Multiple in vitro studies showed that they have antiproliferative, anticarcinogenic and proapoptotic effects. It is also known that they are very susceptible to degradation and their stability is highly influenced by pH, temperature, light and the presence of other phenolic acids.

Unfortunately it is not clear and was not investigated, how exactly anthocyanins act at cellular level and what is their real availability, cell uptake and if they can penetrate skin without degradation.

Current project propose to answer these questions and provide a better understanding of their effects on melanoma cell lines. Its final goal is to determine the exact amount of anthocyanins uptaken by the cell. This information is crucial for pharmaceutical industry in order to develop recommendations for topical application and using anthocyanins as chemopreventive ingredients. Melanoma was chosen because it is responsible for approximately 75 % of all deaths from skin cancers. Considering this, the proposed project brings a new approach to a very common problem in Biotechnology today.

Dezvoltarea unui sistem eco-sustenabil de valorificare a subproduselor industriei berii Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-3622 2020 - 2022

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: https://ancafarcas.wixsite.com/ecos-food-innovation

Abstract:

One of the current development trends of the food industry is focused on diversifying the range of functional products in order to improve the general health of the population. In this sense, current research focuses on identifying sustainable sources of natural active biological compounds, respectively on the recovery of by-products generated by the agri-food industry. Brewers spent grain (BSG) is the insoluble residue generated from the production of wort in the brewing industry. It is estimated that worldwide the annual output is around 30 million tons, about 200 tons of wet spent grain being produced per 10,000 hl of produced beer. This plant-derived by-product is known to contain significant amounts of valuable components, which remain unexploited in the brewing processes. In this sense, the project is focused on developing two functional food products fortified with restructured BSG.

The proposed project aims in a first stage the initial characterization of the selected biomass and the application of treatments to increase the bioavailability of the biologically active compounds. Based on the restructured biomass, two fortified functional food products will be developed and optimized, which will be analyzed from the nutritional, psycho-sensory, microbiological and production costs. Correlations will also be made between product characteristics and total production costs in order to establish the degree of feasibility of new functional foods.

Solid-State Bioprocess Development and Optimization for the Sustainable Production of Powerful Antioxidants from Grape Pomace using Food Grade Fungi Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-1660 2020 - 2022

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO); UNIVERSITATEA TEHNICA DIN CLUJ - NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: https://bioantox2020.wixsite.com/home

Abstract:

The direct disposal of fruit pomaces to soil or landfill causes serious environmental pollution and a significant loss of biomass which can potentially be used to produce various value added metabolites, such as polyphenols (PPh) and terpenoids (ursolic acid-UA). Grape pomace is a winemaking by-product (14 mill. tonnes generated worldwide by beverages industry in 2017) rich in PPh and UA. High amounts of these compounds with high antioxidant and anticancer activities remain in the residues (after processing/extraction) in insoluble bound form (covalently linked to other biomolecules) which confer them poor bioavailability and low intestinal absorption. Liberation and extraction of PPh and UA by enzymes produced by filamentous fungi in solid-state fermentation (SSF) system is a useful technique with perspectives. Therefore, the project aim is to develop and validate an improved production method for a powerful “antioxidants cocktail” consisting of PPh and UA, using grape pomace as substrate, individual and mixed cultures of immobilized spores of Aspergillus niger and Rhizopus oligosporus in SSF system in order to make the process more efficient and to offer to the industry a validated, ready to use recovery technology applicable on large scale. The proposed new SSF systems are cost efficient and offer sustainable opportunities for PPh and UA production via fungal fermentation on cheap and abundant grape pomace. As compared to monocultures, mixed cultures of fungi may lead to better substrate utilization, increased productivity and increased resistance to contamination. Project objectives are: Immobilization of fungi in polyvinyl alcohol (PVA) cryogel; PPh and UA production by SSF of grape pomace with individual and mixed-cultures of immobilized and free spores of A. niger and R. oligosporus; Optimization of production conditions using both fractional order mathematical models and artificial neural network models.

Structural and thermo-oxidative behavior of lipids structured by different mechanisms in process dynamics of several food products and thereof techno-functionality Call name: P 1 - SP 1.1 - Proiecte de cercetare pentru stimularea tinerelor echipe independente
PN-III-P1-1.1-TE-2019-2212 2020 - 2022

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: https://vladmuresan3.wixsite.com/optimogel

Abstract:

The conversion of edible liquid oil (> 90% w) into a gelled structure (i.e., oleogelation) by the means of structurants molecules, without changing the chemical properties of the oil, is receiving a great interest from food researchers in the last few years . This is due to the fact in order to minimise the intake of industrial artificial trans-fat, European commission banned very recently (24 April 2019) the trans-fat content in food by Regulation (UE) 2019/649 and US FDA released its’ final determination on the same issue. Several gelation mechanisms were proposed in the last years, and almost all available vegetable oils were randomly tested for oleogels formulation. However, the limitations of current approaches are related to the fact that the majority of available published papers in Oleogel topic advanced the knowledge mainly by developing and deeply characterizing (microscopic morphology, calorimetry, rheology, texture, etc) the structured lipids obtained while comparing the new oleogels, ONLY with similar reference fats aimed to be replaced (e.g., butter, margarine, animal fat, etc.), and concluding that oleogels match/ do not match desired properties. Although no clear efficient substitution of conventional saturated fat was reported to present, several researchers had randomly selected oleogels formulation and synergic /hybrid combinations for partial or full replacement of regular fat in different food products (margarine, pastry, meat, dairy & confectionery), encouraging results being reported. However, most available literature on the topic, characterize solely oleogels and final products techno-functionalities, being very difficult to optimize the products by deeply understanding oleogels behavior in process dynamics. Consequently, this project aims to systematically assess the structural and thermo-oxidative behavior of lipids structured by different mechanisms in process dynamics of several food products and thereof techno-functionality.

Functional collaboration model between public research organisations and the private sector for high-level scientific and technological services in the bio economy field Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0056 2018 - 2021

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO); STATIUNEA DE CERCETARE -DEZVOLTARE AGRICOLA TURDA (RO); UNIVERSITATEA BABES BOLYAI (RO); UNIVERSITATEA TEHNICA DIN CLUJ - NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: https://sweetconomy.com

Abstract:

The aim of the project is to develop a functional collaboration model between public research organizations and the economic environment in order to provide high-level scientific and technological services in the field of bioeconomics. The objectives of the project are: O1: Creation of a consortium between public research organizations (USAMV, SCDA TURDA, UBB, UTCN) and the economic environment; O2: Increasing institutional capacity through scientific and technological services, through human resources expertise to improve institutional performance; O3: Increasing the institutional capacity to capitalize on the results of the research, by the functioning of the technological transfer component to the economic environment, by publishing and invention patents; O4: Reduction of 30% sugar addition in fermented foods and obtaining a consortium of atomized lactic bacteria. These general objectives are based on the following scientific objectives: Os1: Cultivation of 3 soybean varieties in 3 systems of soil cultivation and characterization of soybean chemical profile; Os2: Evaluation of the toxicological impact of nanoparticles in soybean and the development of a biosensor to identify titanium, zinc and gold nanoparticles accumulated in the plant and grain; Os3: Selection of lactic bacteria and optimization of fermentative processes on soybean and wheat substrate, by mathematical modeling, for the production of polyols to reduce sugar addition in food; Os4: Microinjection of the consortium of lactic bacteria by atomization, envelope aseptic packaging for industrialization; Os5: Educational program on nutrition with soy products, based in the community.The main impact of this proposal is reflected by the creation of new jobs for researchers, sustainable and after the completion of the project, by expertise the human resources and by training the young researchers in modern techniques for the re-launch of SCDA Turda.

New integrated approach for food waste valorization Call name: P 1 - SP 1.1 - Proiecte de cercetare pentru stimularea tinerelor echipe independente
PN-III-P1-1.1-TE-2016-0973 2018 - 2020

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: https://soniasocaci.wixsite.com/intvalwaste

Abstract:

Large amounts of wastes are generated annually by the food industry, their efficient management and valorisation representing one of the main objectives of EU actions against food waste and towards sustainable development. Citrus is one of the most important fruit crop in the world. About 50–60 % w/w of the processed citrus fruits becomes waste which if not processed further, can give rise to serious environmental pollution. CW contains a wide range of bioactive compounds, such as essential oils, carotenoids, fibre, flavonoids, which have many applications in food, cosmetic and pharmaceutical industry. Despite the existing studies on exploitation of bioactive molecules from food wastes, there is still a struggle and need in finding the most efficient methods that could also be economically viable. In this context, the project aims the development of a procedure for the sequential recovery of bioactive compounds from CW, towards achieving the “zero-waste” desiderate. The envisaged bioactive compounds to be recovered from CW, are essential oils (EO), antioxidant compounds (phenolics, flavonoids) and dietary fibres. The performance of the proposed procedure will be assessed (i.e. yield, possibility to be scaled-up) as well as the functionality and bioactivities of recovered biomolecules (i.e. antioxidant capacity, antimicrobial activity, emulsifying properties) using modern analytical techniques. By means of mathematical modelling and statistical analysis of data, the best working parameters will be selected for the design of integrated multi-step system for CW bioactive compounds recovery and valorization.

Anthocyanins pure extracts and their involvement in biological studies: potential bioactive ingredients in nutraceuticals Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-1002 2017 - 2018

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: http://anthoextract.com/

Abstract:

The proposed project brings a new approach to a very common problem in Biotechnology today.

Anthocyanins, natural plants pigments found in very significant amount in edible berries and many vegetables, showed to have high antioxidant activity. Having antioxidant potential, anthocyanins have been highly investigated regarding their health benefits for humans. Unfortunately it is not clear how exactly anthocyanins act at cell level, but their properties seem to be closely related to their antioxidant activity. Even if many researchers are investigating their potential health benefits, almost all bioassays on anthocyanin-rich commodities have been performed using crude anthocyanin extracts from fruits and vegetables, without eliminating other potentially bioactive impurities. These impurities consist mostly of other phenolic compounds and are very likely to have biological effects and may greatly influence biological assays. Based on the fact that anthocyanins are responsible for all potential health benefits the proposed project aimed to develop a low cost method that will allow the obtaining of high purity (>90%) anthocyanin pure extract. Other types of compounds like phenolic acids and flavonoids will be individually separated and recovered from the crude extract. Starting from these pure extracts, comparative tests on cell lines will be performed in order to prove that the benefits of anthocyanin rich materials are due to the anthocyanins themselves or due to other compounds present in those extracts, or even due to the interaction between anthocyanins and other compounds in the crude extracts. The project will offer a complete overview of how much is the real contribution of anthocyanins to the health benefits from anthocyanin-rich materials. Moreover based on the laboratory results, the low cost purification method will be a starting point for industry application offering basic ingredients for next generation of nutraceutical products.

Microsystems for targeted-delivery and controlled-release as alternative therapy of retina diseases – a novel perspective Call name: Projects for Young Research Teams - RUTE -2014 call
PN-II-RU-TE-2014-4-1135 2015 - 2017

Role in this project:

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation:

Project website: http://microreseye.com/

Abstract:

The main objective of this proposal is to tailor a target delivery system that combines the already existing medical procedure for diabetic retinopathy, laser treatment, with the therapeutic effects of the cargo molecules, as resveratrol. Resveratrol will be loaded into biocompatible polyelectrolyte multilayer microcapsules (PEM) and inside the retina pigmented epithelial cells its release will be controlled by a laser procedure. Synthesis and characterization of resveratrol-loaded PEM microcapsules as well as controlled-release optimization procedure of cargo molecules will be followed by in vitro studies in order to observe their cellular trafficking, internalization process and colocalization with RPE cellular structures. Investigation of molecular mechanism modulated by resveratrol loaded into PEM microcapsules is required. This novel controlled-release delivery system for resveratrol could be an alternative for locally administrated anti-vascular endothelial growth factor (anti-VEGF), therapy combined with focal laser photocoagulation, for maintaining RPE function and controlling DR.

Microencapsulated plasmonic nanoparticles for controlled release of bioactive molecules Call name: Projects for Young Research Teams - RUTE -2014 call
PN-II-RU-TE-2014-4-2211 2015 - 2017

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation:

Project website: http://MicroBioNAu.com/

Abstract:

The use of functionalised particles for targeted therapies and imaging, and understanding their effect on cell functions are among the current challenges in nanobiotechnology and nanomedicine. Despite recent advances in functionalising particles for targeted delivery agents and contrast therapies, there is still a great need for nano- and micro-particles that can specifically bind, stabilise and deliver therapeutic agents inside human cells and tissues. The present project addresses two different structures with sizes in the nanoscale and microscale range, namely plasmonic gold nanoparticles and polymeric microcapsules. Basically, the aim of the project is to encapsulate the plasmonic nanoparticles in a biocompatible polymeric matrix with the final goal of reaching controlled and effective release of the loading from the capsules. The research work will be focused on the manufacturing of polymer encapsulated plasmonic nanoparticles, testing ways to open the capsules and release their content, and in vitro testing of biocompatibility of nanoparticle loaded polymeric capsules, their cellular uptake, cellular localisation, distribution inside the cells, and intracellular release. The research activities will make use of modern techniques: high resolution electron microscopy (TEM/SEM), confocal microscopy, molecular spectroscopy techniques (Raman, SERS, FTIR), and performing in vitro experiments on cell cultures.

Anthocyanins liposomes systems and their involvement on molecular signaling of skin diseases Call name: Projects for Young Research Teams - RUTE -2014 call
PN-II-RU-TE-2014-4-0944 2015 - 2017

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: http://antholipoenc.com

Abstract:

Proiectul propus aduce o nouă abordare pentru o problemă de actualitate în domeniul Biotehnologiilor. Antocianii sunt pigmenţi naturali, care au atras interesul nu doar datorită culorilor din fructele şi legume ci şi datorită faptului ca sunt molecule biologic active cu beneficii pentru sănătate. Cele mai bogate surse de antociani sunt fructele de pădure, precum afinele, soc, aronia, zmeură, legumele şi unele cereale. Antocianii prezintă o serie de proprietăţi benefice, dintre care cea mai importantă constă în efectul lor antioxidant.

Fiind o macromoleculă hidrosolubilă, antocianii sunt instabili și foarte sensibili la degradare. Pentru valorificarea potenţialului benefic al acestora, sunt necesare sisteme de livrare controlată şi care le protejează proprietățile. Un bun candidat pentru acesta sunt lipozomii. Incapsularea antocianilor ȋn lipozomi ca sistem de livrare şi protecţie a acestora are două provocări: încapsularea antocianilor în lipozomi nu este bine înțeleasă și documentată. O altă provocare va fi de a evalua potențialul lor antiproliferativ pe celule de melanom după ȋncapsulare. Încapsularea antocianilor în lipozomi, cu o rată ridicată de încapsulare şi păstrarea proprietăților antocianilor după încapsulare va fi de mare folos pentru comunitatea științifică și poate deschide noi posibilitati de utilizare a antocianilor ȋncapsulaţi ȋn lipozomi pentru tratamente alternative în bolile dermatologice.

Composite biomaterials for wound healing based on oxidic nanoparticles and polymeric hydrogels Call name: Projects for Young Research Teams - RUTE -2014 call
PN-II-RU-TE-2014-4-1597 2015 - 2017

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BABES BOLYAI

Project partners: UNIVERSITATEA BABES BOLYAI (RO)

Affiliation:

Project website: https://sites.google.com/site/pniirute201441597alazar

Abstract:

Skin, the largest organ in the body of vertebrates, plays a significant role in preventing the body from many chemical or mechanical damages while impaired wound healing and its medical complications result in a large burden of morbidity and mortality worldwide. Despite extensive research efforts in the field of soft tissue regeneration is still not available an optimal wound dressing for consistent and generalized clinical practice. This proposal focuses on the research and development of new composite biomaterials that contains a polymeric hydrogel (properties: avoidance of open wound infections, maintenance of moisture, absorption and retention of exudates, thermal insulation, incorporation of suitable drug, gas permeability, painless removal and better wound monitor) and a nanoparticulated inorganic silicate biomaterial that contain therapeutic (Ca, Ce) and antibacterial (Ag) agents (properties: accelerate the healing of full-thickness dermal wounds, avoiding infections and enhancing of the proliferation and migration of fibroblasts, keratinocytes and vascular endothelial cells). The combinations of these materials in order to bring together their specific properties represent the highlight of this project. Moreover in the best of the project leader’s knowledge a composite biomaterial in the presented combination was never used in wound healing applications.

TESTING THE ANTIOXIDANT, ANTIPROLIFERATIVE AND PROAPOPTOTIC PROPERTIES OF BLUEBERRY POLYPHENOL EXTRACT ON TUMORAL CELL CULTURE Call name: Projects for Young Research Teams - TE-2010 call
PN-II-RU-TE-2010-0168 2010 - 2013

Role in this project:

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA DIN CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA DIN CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA DIN CLUJ-NAPOCA (RO)

Project website: http://www.usamvcluj.ro/TE 186 AFINE/

Abstract:

IN OUR COUNTRY, BLACK BLUEBERRIES (VACCINIUM MYRTILLUS L.) GROW IN HILLS ACID AREAS (MAJORITY) BUT IS ALSO FOUND IN CULTURE. BESIDES THEIR WATER, SUCROSE, PROTEIN AND PECTIC SUBSTANCES CONTENT, BLUEBERRIES CONTAIN A HIGH CONCENTRATION OF ANTIOXIDANTS WHICH NEUTRALIZE FREE RADICALS RESPONSIBLE FOR THE PRODUCTION OF DEGENERATIVE DISEASES. DUE TO MULTIPLE BENEFICIAL EFFECTS ON HUMAN HEALTH OF BLUEBERRIES (IMPROVE EYESIGHT, PREVENT MACULAR DEGENERATION, ANTIDIABETIC AND ANTITUMOR PROPERTIES), THEY ARE CONSUMED FREQUENTLY IN NATURALLY FORM AND AS VARIOUS PREPARATIONS (SUPPLEMENTS NUTRITIONAL).

RESEARCH SHOWS THEIR CYTOTOXIC EFFECT (BY VIABILITY CELL) ON VARIOUS TUMOR CELLS: COLON, PROSTATE AND LIVER CELLS.

THERE IS NO LITERATURE DATA RELATING ON CYTOTOXIC EFFECT TO THE BLUEBERRY EXTRACT ON OVARIAN AND BREAST TUMOR CELLS.

THE MAIN PURPOSE OF THIS PROJECT IS TESTING THE ANTIOXIDANT EFFECT OF TOTAL POLYPHENOL EXTRACT AND THE ANTHOCYANINS EXTRACT AND THEIR PROAPOPTPTIC EFFECT ON OVARIAN AND BREAST TUMOR CELLS.

THIS WILL BE ACHIEVED BY FURTHER AIMS:

O1-THE EXTRACTION FLAVONOIDS BIOACTIVE COMPOUNDS AND EXTRACTS CHARACTERIZATION USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC (HPLC)

O2–DOSING THE BIOACTIVE COMPOUNDS: TOTAL POLYPHENOLS, TOTAL ANTHOCYANINS, TOTAL FLAVONOIDS

O3 - EVALUATION IN VITRO OF ANTIOXIDANT ACTIVITY FOR THE EXTRACTS USING ANTIOXIDANT METHODS

O4 - TESTING THE CYTOTOXIC EFFECTS OF THE TOTAL EXTRACTS AND ANTHOCYANIN FRACTION EXTRACT ON OVARIAN AND BREAST TUMOR CELLS.

O5-EVALUATION OF ANTIOXIDANT STATUS OF THE CELLS TREATED WITH BLUEBERRY EXTRACT

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List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
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Peer-review activity for international programs/projects