BrainMap

Mrs. Victoria Cecilia Cristea

PhD. Habil.

Professor - UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU"

Researcher | Teaching staff | Scientific reviewer | PhD supervisor

Web of Science ResearcherID: not public

Curriculum Vitae (20/02/2024)

Expertise & keywords

European integration of new technologies and social-economic solutions for increasing consumer trust and engagement in seafood products Call name: EC - Horizon Europe
Horizon-241861-101060712 2022 - 2026

Role in this project: Partner team leader

Coordinating institution: Institution

Project partners: WRG EUROPE LTD (GB); EUROPEAN AQUACULTURE SOCIETY (BE); INSTITUT JOZEF STEFAN (SI); DE LA CUEVA GONZALEZ COTERA JAVIER (ES); INSTITUTO PORTUGUES DO MAR E DA ATMOSFERA IP (PT); UNIVERSITATEA DE MEDICINA SI FARMACIE IULIU HATIEGANU CLUJ-NAPOCA (RO); UNIVERSITA DEGLI STUDI DI FIRENZE (IT); UNIVERSITA DEGLI STUDI DI PADOVA (IT); MICRUX FLUIDIC SL (ES); CLUSTER DE LA ACUICULTURA DE GALICIA ASOCIACION (ES); EUROPEAN FOOD INFORMATION RESSOURCE AISBL (BE); REDINN - SRL (IT); AQUABIOTECH LIMITED (MT); BUGENVILA INVESTICIJE DOO (HR); DANMARKS TEKNISKE UNIVERSITET (DK); ALMA MATER STUDIORUM - UNIVERSITA DI BOLOGNA (IT); PREDUZECE ZA INFORMACIONE TEHNOLOGIJE I ELEKTRONSKO TRGOVANJE BELIT DOO (RS); OXYGUARD INTERNATIONAL AS (DK); BRANDENBURGISCHE TECHNISCHE UNIVERSITAT COTTBUS-SENFTENBERG (DE); DRUSTVO SA OGRANICENOM ODGOVORNOSCU DIGITALSMART DOO PODGORICA (ME); NORCE NORWEGIAN RESEARCH CENTRE AS (NO); INTERNATIONAL ORGANISATION FOR THE DEVELOPMENT OF FISHERIES AND AQUACULTURE IN EUROPE (DK)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE IULIU HATIEGANU CLUJ-NAPOCA (RO)

Project website:

Abstract:

European integration of new technology & socio-economic solutions for increasing consumer trust and engagement in seafood products Call name: P 5.8 - SP 5.8.1 - Premiere Orizont Europa - Instituții - Competiția 2023
PN-IV-P8-8.1-PRE-HE-ORG-2023-0076 2023 - 2025

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU"

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Project website:

Abstract:

FishEUTrust represents a consortium of twenty-two organizations from fourteen countries, pooling their expertise to defragment the current food system to ensure sustainability and deliver solutions for a transparent and traceable seafood supply chain necessary to promote high-end, pan-European farmed seafood. The innovation at the heart of FishEUTrust is integrating different actors into a digital platform that links technology providers, supply chain stakeholders, regulatory/policymakers and consumers. FishEUTrust will establish five Co-creation Living Labs (CLLs) in diverse environments: the Mediterranean Basin, the North Sea and the Atlantic Sea.

These CLLs will enable user involvement in innovation and development processes and act as demonstrators for the consortium to test and validate digital and non-digital supply chain solutions. Examples include creating sustainable business models, exploiting IPR

strategies for aquaculture, e.g., protecting cultural and culinary heritage, short food supply chains, exploiting underused fish species, and innovative engaging activities to stimulate/nudge behavioural change. It will also develop tools for maximizing trust by

guaranteeing the quality, safety, and traceability of seafood products based on smart control systems (sensors), a suite of tools integrating metagenomics, genetic biomarkers, isotopic techniques, and digital technologies (labelling, Product Passport/

Blockchain). These tools will be integrated into a single cutting-edge digital FishEUTrust platform that will apply the latest in artificial intelligence, data science and human-computer interactions. Finally, an integrated impact assessment and life cycle analysis will be performed to quantify the environmental footprint, health sustainability, and socio-economic benefits of FishEUTrust solutions.

FishEUTrust will be supported by a comprehensive scientific, public, policymaker and industrial dissemination plan to communicate results to a broad audience.

DNA-miniaturised sensors array gold-chip for Aflatoxin and Ara h allergen simultaneous electrochemical detection for food safety management Call name: P 1 - SP 1.1 - Proiecte de cercetare Postdoctorală
PN-III-P1-1.1-PD-2019-0631 2020 - 2024

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU"

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Project website: https://dnasens4safefood.wixsite.com/mysite

Abstract:

Nowadays, foodborne illnesses and allergies have become a global menace on public health. According to World Health Organization (WHO) there are four leading causes of foodborne diseases: the presence of viruses, harmful bacteria, parasites or chemicals. The latter is further categorised into aﬂatoxins, arsenic, cassava cyanide, peanut allergens and dioxins. Moreover, WHO identiﬁed the “groups of chemicals and toxins that are of highest priority in estimating the burden of foodborne disease” as there are no proper monitoring systems yet developed. Food contaminants and allergens affect the health status and represent possible threats for both humans and animals being identified as severe food safety hazards; therefore, their careful management in food industries and food control agencies is of high importance to ensure controlled food safety management systems and thereby safe health strategies.

The main objective of DNASens4SafeFood project consists in facilitating the determination of aflatoxin B1 (AFB1, mycotoxin) and Ara h1 (allergen) by sensitive and selective electrochemical detection of their concentrations from different food products, in order to diminish the occurrence of food-induced diseases and allergic symptoms among sensitized subjects and thus improve their life-quality. The originality of the project consists in the development of DNA-miniaturized sensors array gold-chip (32-electrode/chip) which is portable, inexpensive and non-toxic to be used as a tool with multiplexing capabilities for the simultaneous detection of low concentrations (ng/L) of AFB1 and Ara h1 for the on-site real-time analysis.

Thus, the development of an easy-to-use, fast and accurate device is fundamental to prevent food safety hazards caused by the consumption of unsafe food products which can lead to development of hepatic, cancerous, immunosuppressive or neurological diseases.

Border detection of illicit drugs and precursors by highly accurate electrosensors Call name:
833787 2019 - 2023

Role in this project: Partner team leader

Coordinating institution: University of Antwerp

Project partners: University of Antwerp (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Project website: http://www.bordersens.eu

Abstract:

Combining robust sensor technologies with the inherent advantages of electrochemical strategies, nano-molecularly imprinted polymers, and multivariate and pattern data analysis, BorderSens will enable highly accurate selective detection of trace levels of illicit drugs and precursors. With borders being important gateways for the entrance of illicit drugs and their precursors, custom and border control authorities are facing pertaining challenges to detect such dangerous substances and safeguard the public. The main challenges posed by currently used on-site methods to detect illicit drugs and precursors are low accuracy, in the case of colour tests, and high cost and low portability, in the case of spectroscopic tests. In the light of a pressing need for better drug test systems at EU borders, the ultimate research aim of the BorderSens is to develop a portable, wireless single prototype device with the capability to quickly test for different types of drugs, precursors and adulterants/cutting agents, with outstanding accuracy and reduced false positives and false negatives. BorderSens will demonstrate the innovative technological solutions at seven demonstrations sites at EU borders with end-users and ensure exploitation plans guaranteeing strong impact. BorderSens brings together universities, a big manufacturer of electrochemical sensors, a specialised SME, ten end-users i.e. forensic institutes, police forces and border authorities, and a high quality external advisory board, to provide an excellent scientific-technical perspective and a straightforward exploitation route, with great impact on the safety of EU citizens.

Pathogen Contamination Emergency Response Technologies Call name: EC - H2020
H2020-229396-883484 2020 - 2023

Role in this project: Partner team leader

Coordinating institution: UNIVERSITY OF CYPRUS

Project partners: UNIVERSITY OF CYPRUS (CY); ETHNIKO KENTRO EREVNAS KAI TECHNOLOGIKIS ANAPTYXIS (EL); WATER EUROPE (BE); COMMUNICATION AND INFORMATION SYSTEMS DIRECTORATE (BG); AQUA-Q AB (SE); KWR WATER B.V. (NL); ETAIRIA HYDREFSIS KAI APOCHETEFSIS THESSALONIKIS AE (EL); SUNDOSOFT LTD (KR); UNIVERSITATEA DE MEDICINA SI FARMACIE IULIU HATIEGANU CLUJ-NAPOCA (RO); MINISTRY OF INTERIOR (CY); Korea Institute of Science and Technology (KR); ELLINIKI OMADA DIASOSIS SOMATEIO (EL); NATIONAL TECHNICAL UNIVERSITY OF ATHENS - NTUA (EL); MicroLAN (NL); CETAQUA, CENTRO TECNOLOGICO DEL AGUA, FUNDACION PRIVADA (ES); AYUNTAMIENTO DE GRANADA (ES); FUNDACIO EURECAT (ES); SATWAYS - PROIONTA KAI YPIRESIES TILEMATIKIS DIKTYAKON KAI TILEPIKINONIAKON EFARMOGON ETAIRIA PERIORISMENIS EFTHINIS EPE (EL); COLLABORATING CENTRE ON SUSTAINABLE CONSUMPTION AND PRODUCTION GGMBH (DE); STICHTING WATERNET (NL); FRAUNHOFER GESELLSCHAFT ZUR FOERDERUNG DER ANGEWANDTEN FORSCHUNG E.V. (DE); PHOEBE RESEARCH AND INNOVATION LTD (CY); ENGINEERING - INGEGNERIA INFORMATICA SPA (IT); FUNDACION CENTRO ANDALUZ DE INVESTIGACIONES DEL AGUA (ES); EMPRESA MUNICIPAL DE ABASTECIMIENTO Y SANEAMIENTO DE GRANADA SA (ES); UNIVERSITY OF STUTTGART (DE); LABAQUA SA (ES)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE IULIU HATIEGANU CLUJ-NAPOCA (RO)

Project website:

Abstract:

Border detection of illicit drugs and precursors by highly accurate electrosensors Call name: P 3 - SP 3.6 - Premierea participării în Orizont 2020
PN-III-P3-3.6-H2020-2020-0095 2020 - 2022

Role in this project: Partner team leader

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU"

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Affiliation:

Project website: https://bordersens.eu/

Abstract:

Combining robust sensor technologies with the inherent advantages of electrochemical strategies, nano-molecularly imprinted polymers, and multivariate and pattern data analysis, BorderSens will enable highly accurate selective detection of trace levels of illicit drugs and precursors. With borders being important gateways for the entrance of illicit drugs and their precursors, custom and border control authorities are facing pertaining challenges to detect such dangerous substances and safeguard the public. The main challenges posed by currently used on-site methods to detect illicit drugs and precursors are low accuracy, in the case of colour tests, and high cost and low portability, in the case of spectroscopic tests. In the light of a

pressing need for better drug test systems at EU borders, the ultimate research aim of the BorderSens is to develop a portable, wireless single prototype device with the capability to quickly test for different types of drugs, precursors and adulterants/cutting agents, with outstanding accuracy and reduced false positives and false negatives. BorderSens will demonstrate the innovative technological solutions at seven demonstrations sites at EU borders with end-users and ensure exploitation plans guaranteeing strong impact. BorderSens brings together universities, a big manufacturer of electrochemical sensors, a specialised SME, ten end-users i.e. forensic institutes, police forces and border authorities, and a high quality external advisory board, to provide an excellent scientific-technical perspective and a straightforward exploitation route, with great impact on the safety of EU citizens.

Development of laccase-based biosensor/cell constructions to monitor catecholamine release from in situ neuronal cells under hypoxia conditions Call name: P 1 - SP 1.1 - Proiecte de cercetare Postdoctorală - PD-2021
PN-III-P1-1.1-PD-2021-0156 2022 - 2022

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA

Project partners: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA (RO)

Project website: https://infim.ro/en/project/lacccell/

Abstract:

The research project proposes the development of laccase-based biosensors to monitor in situ the dynamic change in catecholamine concentration released from neuronal living cells under hypoxia conditions. The biosensor/cell construction incorporates living cells at the biosensor surface in direct contact with the enzymatic layer. Recently, electrochemical real-time monitoring of analytes within and/or released from living cells has provided important solutions to medical diagnosis and disease treatment. The importance of catecholamines (dopamine and norepinephrine) in the regulation of various physiological functions, turns these species into interesting analytical targets, with elevated levels leading to neurological or psychiatric disorders. The developed device is envisaged to be a suitable platform for cell growth and proliferation, with an embedded selective and sensitive mechanism based on the oxidation by the laccase enzyme of secreted dopamine and norepinephrine under external stimuli to the cell environment . The proposed mechanism has the potential to be applied for the external stimuli involvement investigation on exocytosis of neuronal living cells. This project comes in response to the need of in situ evaluation of the dynamic changes in neurochemical pathways, which remains to be fully explored.

Electrochemical detection of bacterial quorum sensing molecules and biofilm formation Call name: P 1 - SP 1.1 - Proiecte de cercetare pentru stimularea tinerelor echipe independente
PN-III-P1-1.1-TE-2019-1360 2020 - 2022

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU"

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Project website: https://sites.google.com/view/quorel-sens/home

Abstract:

Quorum sensing (QS) plays an important role in the pathogenesis of certain bacteria due to its involvement in virulence factors synthesis and formation of the biofilm, which is resistant to the immune system and to antibiotics. QS is a form of cell to cell communication between bacteria using substances called autoinducers, such as N-acyl derivatives of homoserine lactone (HSLs) or cyclic dimeric guanosine monophosphate (c-di-GMP), the latter being involved in biofilm formation.

The main purpose of this project consists in facilitating the fast identification of nosocomial infections and their proper antibiotic treatment, by electrochemical detection of molecules involved in QS and biofilm formation from microbiological cultures and different biological samples.

The QUOREL-SENS project includes several goals: development of a global strategy for obtaining electrochemical biomimetic sensors capable of (1) selective and sensitive detection of QS in P. aeruginosa, (2) of the biofilm formed by P. aeruginosa, and of (3) simultaneous electrochemical evaluation of the concentration of QS molecules or biofilm formation and the concentration of different antibiotics.

The envisioned goals will be achieved by two different, interconnected strategies, with specific objectives: (1) modification of the electrodes with molecularly imprinted polymers (improving the selectivity) and with nanomaterials (improving the electrochemical properties and the sensitivity) for the detection of HSLs and of c-di-GMP produced by P. aeruginosa; (2) development of electrochemical aptasensors for the detection of HSLs and of c-di-GMP; (3) evaluation of the concentration of molecules involved in QS or biofilm formation in the presence of different antibiotics, their concentration being detected electrochemically before and after exposure to antibiotics; (4) complete characterization of the developed electrochemical sensors and their adaptation for real samples analysis.

Synergy of plasmonic structures, affinity elements and photosensitizers for electrosensing of pharmaceuticals Call name: P 3 - SP 3.2 - Proiecte ERA.NET
ERANET- RUS-PLUS-PLASMON-ELECTROLIGHT 2018 - 2021

Role in this project: Partner team leader

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU"

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO); University of Antewerpen, Antwerp, Belgium (BE); Federal State University M.V. Lomonosov, Moscow, Russia (RU)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Project website: https://eranetrusplus.wixsite.com/plasmonelectrolight

Abstract:

The main objective of the PLASMON-ELECTROLIGHT project is to elaborate an efficient sensor strategy to measure pharmaceuticals in surface water near big cities, farms, pharmaceutical industry, etc. The detection

technique will be developed from an original photoelectrochemical detection strategy that is boosted by advanced photosensitizers, plasmonic enhancement, and affinity recognition.

Recently, we have developed an original electrochemical detection principle based on photocatalytic

oxidation mediated by singlet oxygen (Trashin et al., Nature Communications, 2017). We applied this

strategy for the detection of the antibiotic amoxicillin at the concentration level of 20 nM. Within this new

project we will now explore and advance the detection strategy, by preparing more efficient photoactive

materials (gaining sensitivity) and by introducing affinity elements (gaining selectivity).

The photoactive hybrid materials must be designed carefully through rational choice of photosensitizers and metallic nanostructures, theoretical modeling, and experimental correlations. Next, the materials will be combined with biorecognition using aptamers and employed for preparation of a photoelectrochemical

sensor. One of the important features of the photoelectrochemical detection strategy is that the signal and background can be simultaneously measured in the presence of an analyte by simply switching light on and off. This makes the measurement more robust in changeable medium even without or minimal sample preparation.

Beside elaboration the superior sensing strategy for environmental monitoring, our objectives include a better understanding of the mechanism for plasmonic enhancement of photosensitizers’ activity, developing new photoreactive materials and better methods to tests them. This will contribute to different field of chemical

sensing, material science, and energy conversion.

Smart materials for medical applications Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0407 2018 - 2021

Role in this project: Partner team leader

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); CENTRUL DE CHIMIE ORGANICA AL ACADEMIEI ROMANE "C.D.NENITESCU" (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE "CAROL DAVILA" (RO); UNIVERSITATEA BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE CHIMICO - FARMACEUTICA - I.C.C.F. BUCURESTI (RO); Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE "GRIGORE T. POPA" DIN IAŞI (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Project website: https://intelmatupb.wixsite.com/intelmat

Abstract:

INTELMAT project represents advanced research in the field of synthesis and application of smart materials for medical engineering in order to solve essential features of some acute/chronic diseases of large occurrence. 5 thematic directions are taking into consideration: 1. Developing of a controlled-release system of complex micro-colloidal architectures based on bacterial cellulose and hydrogels used for management of chronically wounds which aims to overcome the limitations of classical treatments; 2. Developing of new biomaterials specially designed with targeted action for treatment of inflammatory diseases of gastrointestinal segment; 3. Synthesis of new generation of composite membrane materials for artificial kidneys based on biocompatible polymers and derivative graphene; 4. Developing of some auto-assembly 3D platforms with controlled-released drugs based on polymeric nanoparticles and composite nanogels for the therapy of colon-rectal cancer; 5. Developing of innovative technologies for the synthesis of some 1D nano-architectures (nanowires) with controlled morphology, with applications in producing of non-enzimatic electrochemical biosensors.

High accuracy innovative approach for the robotic assisted intraoperatory treatment of hepatic tumors based on imagistic-molecular diagnosis Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0221 2018 - 2021

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA TEHNICA DIN CLUJ - NAPOCA

Project partners: UNIVERSITATEA TEHNICA DIN CLUJ - NAPOCA (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO); INSTITUTUL DE MECANICA SOLIDELOR (RO); INSTITUTUL REGIONAL DE GASTROENTEROLOGIE - HEPATOLOGIE PROF. DR. OCTAVIAN FODOR CLUJ-NAPOCA (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU OPTOELECTRONICA INOE 2000 INCD (RO)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Project website: https://cester.utcluj.ro/improve/home

Abstract:

The IMPROVE project will be developed by a transdisciplinary group, which integrates the knowledge of multiple specialists from the technical field (such as mechanical engineers, computer science engineers and material sciences engineers), a team of medical doctors (surgeons, gastroenterologists, oncologists, and radiologists), and a team of chemical scientists. The IMPROVE group will develop an innovative solution for the treatment of the patients diagnosed with non resectable hepatic tumors, based on a novel robotic system, and an novel medical imaging diagnostic tool. The majority of the hepatic tumors are not resectable due to a variety of factors such as tumor placement, size, and the patient general condition. In order to deliver a better palliative care towards the patient, and a better quality of life, the IMPROVE project proposes an integrated system to deliver treatment in the form of brachytherapy or chemotherapy. The treatment will be delivered in a minimally invasive manner, guided by a robot (which delivers the treatment agent through needles), based with a non-invasive diagnostic method. The diagnosis stage consists of the development of “smart” image analysis algorithms which can detect and define some hepatic diseases such as cancers. The preplanning stage will define the treatment method as well as the needle trajectories. For the treatment delivery a robotic structure will be developed to help the clinicians in the minimally invasive (laparoscopic) in situ procedure. The treatment agent (brachytherapy of chemotherapy) will be precisely delivered within the tumor using specialized instruments (for needle insertion and delivery) guided by the robotic system. The IMPROVE team is composed out of experienced researchers whit excellent results in the medical field, and well based former collaborations. The group synergy represents a key factor in obtaining positive results in the survival outcome of the hepatic cancer patients.

Innovative Tehnological Approaches for validation of Sailvary AGEs (Advanced Glycation End Products) as novel biomarkers in evaluation of risk factors in diet related diseases Call name: P 3 - SP 3.2 - Proiecte ERA.NET - COFUND
COFUND-ERA-HDHL ERANET -SALIVAGES 2017 - 2020

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU"

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Project website: http://www.umfcluj.ro/component/content/article/8-ro/508-proiect-salivages?Itemid=216

Abstract:

Advanced Glycation End Products (AGEs) may exert negative effects on the human body by altering

structure/function of proteins and by activating selective receptors. We have recently contributed to demonstrate

the role of diet in producing different patterns of AGEs and to elucidate the mechanisms by which AGEs mediate

diet-induced metabolic changes. Nevertheless, the role of salivary AGEs as early biomarkers of transition towards

diet-related disease has never been proposed.

AIMS: The main aims of SALIVAGES are: (i) the identification of the main AGEs modulated by diets, focusing

on saliva as a highly accessible source of AGEs, and (ii) the validation of non-invasive methods to detect salivary

AGEs as biomarkers of the risk of developing diet-related diseases.

WORK PLAN: Original models will be developed to study AGEs accumulation in oral mucosa, gut and adipose

tissue and the related activation of selective molecular pathways. Advanced multidimensional analytical platforms

(GC×GC-MS, HPLC-MS/MS) and highly sensitive biosensors will be used to comparatively detect AGEs in

different biofluids (saliva, blood, urine). Metabolite profiling as well as targeted and untargeted fingerprinting on

multidimensional analytical data will support the investigation overtaking reductionist vision limited to single

biomarker discovery. Innovative glycomic analyses of biological samples will allow the study of glycosylation

changes in response to AGEs and the identification of putative glycobiomarkers. Novel recognition molecules and

biomimics will subsequently be developed. Analysis on salivary AGEs from healthy subjects will allow to verify

whether results emerged from experimental work are relevant for humans and to identify specific AGEs as

biomarkers with relevant clinical information potential. Salivary volatilome will be profiled to define correlations

with AGEs distribution.

IMPACT OF THE EXPECTED RESULTS: The expected results will contribute to promote the development of

innovative diagnostic approaches to classify individual patterns and/or establish the degree of health changes.

These results will be achieved by multidisciplinary approaches based on the integration of preclinical and clinical

studies, analytical and food chemistry, metabolomics and information technologies. Overall, SALIVAGES will

contribute to reveal new horizons in the identification of strategies for monitoring key biological processes

underlying the nutrition-health relationship.

Development of electrochemical sensors for monitoring the vancomycin and gentamicin treatments Call name: P 1 - SP 1.1 - Proiecte de cercetare Postdoctorală
PN-III-P1-1.1-PD-2016-1132 2018 - 2020

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU"

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Project website: https://sites.google.com/view/vangeldet

Abstract:

Vancomycin and gentamicin are antibiotics widely used for the prophylaxis and treatment of severe infections with Gram-positive and negative bacteria, respectively, but their use can lead to ototoxicity, nephrotoxicity and bacterial resistance to antibiotics, needing a therapeutic drug monitoring of these antibiotics that maximizes efficacy, reduces toxicity and avoids the under-dosing, reducing the antibiotic resistance.

The main purpose of the project consists in facilitating the monitoring of vancomycin and gentamicin treatments, by their electrochemical detection from biological samples (serum, urine), in order to help the proper use of these antibiotics, diminishing their toxicitiy and impairing the development of antibiotics resistance.

The project objectives are to develop a global strategy for obtaining portable, inexpensive, non-toxic, electrochemical (bio)sensors, able to selectively detect low concentrations of vancomycin and gentamicin (ng/L), to adapt these (bio)sensors for simultaneous detection of the two antibiotics and for the routinely monitoring of vancomycin and gentamicin treatments, replacing the chromatographic, spectrophotometric and immunochemical methods, that are expensive, time-consuming and inadaptable for bedside testing.

The objectives will be achieved by three interconnected strategies: the direct electrochemical detection of vancomycin and gentamicin, taking into account that both of them present electroactive functional groups, the modification of the electrodes with nanomaterials and polymeric films, leading to better electrochemical properties, with an increase of the sensitivity of the analysis and the development of selective biosensors using as biorecognition elements antibodies or peptides, capable to interact specifically with the target molecules. Different electrode modification strategies will be tested, increasing the chances of successful development of highly selective and sensitive electrochemical (bio)sensor.

Nano-Enabled Optical Fiber Biosensor Device with Smartphone Interface for Fast and Selective Detection of Antibiotics in Water Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-0172 2017 - 2018

Role in this project: Partner team leader

Coordinating institution: UNIVERSITATEA TEHNICA DIN CLUJ - NAPOCA

Project partners: UNIVERSITATEA TEHNICA DIN CLUJ - NAPOCA (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Project website: http://bel.utcluj.ro/~galatusr/PED67UEFISCDI.html

Abstract:

The water quality of the water subsystem network is important to be monitored in order to protect the public health and the local ecosystems from the harmful effects of the substances with risks to human and wildlife organisms. The European Union follows the strategic plan implementation of the Water Framework Directive 2000/60/EC. Current EU guidance on the implementation of the Water Supply Regulations for water quality monitoring includes the option for using online instrumentation to gather compliance data, rather than using sampling and laboratory analysis. Efficient tools for rapid detection of antibiotics in water are urgently needed and represents the core of the proposal. In the US alone, every year 3400 tons of antibiotics are used on patients, while 8900 tons are used on animals-2015. Up to 85% unmetabolized antibiotic is excreted in urine/feces by the human body On livestock animals are consumed more than twice the amount of antibiotics than on humans. Evidence of antibiotic resistant microorganisms has been found in surface wastewater, groundwater and drinking water. Based on a 2013 report regarding the sales in the community pharmacies of the systemic antibiotics from J01 class, Romania is the third biggest consumer of antibiotics in the EU. The present proposal aims to develop a water quality sensor system (WQSS) by integrating the facilities of the optical fibers technology and smartphone. The system is based on low-cost fiber optic sensors (FOS), with nanostructured plasmonic area, SPR(gold, Molecularly Imprinted Polymer and graphene). FOS, being intrinsically robust, reliable and immune to electromagnetic interference, are especially suitable for in situ real-time detection, being fully compatible with out-of-lab continuous monitoring. FOS offer strong arguments over traditional methods of sensing. Based on the expertise of two partners, the project will start at TRL2 and will end at TRL4 with SPR sensor integration remote monitoring station(WOSS).

Design sensing platforms for detection and quantification of biomarkers involved in neurological disorders Call name: Projects for Young Research Teams - RUTE -2014 call
PN-II-RU-TE-2014-4-0460 2015 - 2017

Role in this project:

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE (U.M.F) Cluj-Napoca

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE (U.M.F) Cluj-Napoca (RO)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE (U.M.F) Cluj-Napoca (RO)

Project website: http://www.granturi.umfcluj.ro/BIO-NEURO-SENS

Abstract:

Neurological disorders are numerous, diverse, frequently severe, affecting a large portion of the world’s population, with challenges in their diagnosis and treatment. The current project proposes the development of a global strategy for designing (bio)sensors, involving an ultrasensitive electrochemical setup based on a specific and selective nanoplatform capable of simultaneous detection of some biomarkers involved in neurological disorders. The originality of the proposed project resides from the use of electroanalytical methods involving electrodes modified in a controlled and optimized way with a novel association of nanomaterials with bioelements. Various strategies are envisaged for the functionalization of different types of electrodes and the use of various nanomaterials, design of new and innovative way that will allow their immobilization. The obtainment of the completely functional nanoplatforms will be followed by the optimum immobilization of the bioelements (enzyme, antibody, aptamer) will be determined, assuring highly specific detection. After the complete characterization of the elaborated sensors in terms of properties and performances, their application for real samples analyses will be tested, as a first step in development of point-of-care devices.

The success of the project could assure an early diagnosis of neurological disorders with improvement in the health of the population.

AMPEROMETRIC IMMUNOSENSOR FOR OVARIAN AND UTERINE CANCER BIOMARKERS Call name: Exploratory Research Projects - PCE-2011 call
PN-II-ID-PCE-2011-3-0355 2011 - 2016

Role in this project: Key expert

Coordinating institution: Universitatea de Medicina si Farmacie ˝Iuliu Hatieganu˝ Cluj-Napoca

Project partners: Universitatea de Medicina si Farmacie ˝Iuliu Hatieganu˝ Cluj-Napoca (RO)

Affiliation:

Project website: http://www.granturi.umfcluj.ro/id338

Abstract:

The MUC1 gene encodes a type-I transmembrane glycoprotein that is expressed on the apical surface of most simple epithelia, including mammary gland, female reproductive tract, lung, kidney, stomach, gall bladder, and pancreas as well as some non-epithelial cell types.

Recent immunological studies suggest that MUC1 is a potential marker and a prognosis factor in diagnosis of ovarian cancer and endometriosis.

It is proposed the elaboration of a novel series of immunosensors based on MUC1 and tumor MUC1 proteins, which can be a specific and sensitive alternative to currently more or less invasive, immunohistochemical methods actually used. Once the tumor proteins in blood successfully detected, will be followed by the design and construction of a noninvasive, specific and sensitive diagnostic device for biological fluids.

The main objectives are: 1). Development of new immunosensing devices; a. Electroactive surface design (fabrication of gold and carbon based screen-printed electrodes functionalized by thiolic compounds, carbon nanotubes, graphenes as nanobiolabels); b. Bioelement design (choice of antibodies and/or aptamers); c. Immunosensor development by different immobilization methods (biotin-avidin, selfassembling, conducting polymers); 2). Electroanalytical characterization of the new immunosensors (electrode surface characterization, electroanalytical performances); 3). Immunoelectrochemical method elaboration and prevalidation (design and optimization of the immun

project title Call name: P 1 - SP 1.1 - Proiecte de mobilitate pentru cercetatori
PN-III-P1-1.1-MC-2017-0506 2017 -

Role in this project:

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU"

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Project website:

Abstract:

Innovative electrochemical sensors for sensitive and selective detection of some biogenic amines Call name: P 3 - SP 3.1 - Proiecte de mobilități, România-Franța (bilaterale)
PN-III-P3-3.1-PM-RO-FR-2016-0003 2016 -

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE (U.M.F) Cluj-Napoca

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE (U.M.F) Cluj-Napoca (RO); Université Rennes 1, Campus de Beaulieu, nstitut des Sciences Chimiques de Rennes (ISCR), UMR CNRS 6226, "Matière Condensée et Systèmes Electroactifs" (MaCSE) (FR)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE (U.M.F) Cluj-Napoca (RO)

Project website:

Abstract:

Aminele biogene (dopamina, serotonina) prezintă un rol important în controlul și reglarea principalelor funcții neuronale, fiind incriminate de apariţia unor patologii psihologice și neurologice. Acești compuși pot fi consideraţi atât biomarkeri în apariția și monitorizarea evoluţiei unor boli neurodegenerative cât și ținte pentru noi metode terapeutice. În vederea diagnosticării și monitorizării eficacității terapiei propuse, se impune dezvoltarea unor metode de detecție și cuantificare rapide ale acestor amine. Proiectul propus urmărește obținerea unor platforme selective pentru detecția specifică a aminelor biogene cum sunt dopamina sau serotonina. Astfel, posibilitatea implementării unor metode și dispozitive care să permită identificarea și cuantificarea acestor compuși rapid, cu costuri scăzute și fără a necesita separarea în cazul determinărilor efectuate pe matrici complexe, este de mare interes pentru lumea medicală. Originalitatea acestui studiu constă în implementarea unor platforme pe bază de nanoparticule de aur si polipirol care să permită analiza calitativă și cantitativă a unor amine biogene prin intermediul tehnicilor electrochimice precum si a unor catalizatori biomimetici pe baza de cupru. Tehnicile abordate permit sintetizarea unor nanostucturi care prezintă densitate crescuta de centrii activi disponibili pentru legarea analitului de interes pe o suprafaţă geometrică relativ redusă și posibilităţi de miniaturizare cu creşterea spectrului de aplicaţii.

project title Call name: P 1 - SP 1.1 - Proiecte de mobilitate pentru cercetatori cu experienta din diaspora
PN-III-P1-1.1-MCD-2016-0101 2016 -

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE (U.M.F) Cluj-Napoca

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE (U.M.F) Cluj-Napoca (RO)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE (U.M.F) Cluj-Napoca (RO)

Project website:

Abstract:

Call name: Premierea obtinerii atestatului de abilitare - Competitia 2015
PN-II-RU-ABIL-2015-2-0008 2015 -

Role in this project:

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE (U.M.F) Cluj-Napoca

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE (U.M.F) Cluj-Napoca (RO)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE (U.M.F) Cluj-Napoca (RO)

Project website:

Abstract:

FUNCTIONALIZED NANOMATERIALS WITH CHROMO AND FLUOROPHORES AND NANOSTRUCTURED PLATFORMS FOR BIOSENSORS APPLIED IN PHARMACEUTICAL AND BIOMEDICAL FIELDS Call name: Programul de actiuni Integrate Romania-Franta (bilaterale)
PN-II-CT-RO-FR-2014-2-0008 2014 -

Role in this project:

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE (U.M.F) Cluj-Napoca

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE (U.M.F) Cluj-Napoca (RO); Universite Joseph Fourier (FR)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE (U.M.F) Cluj-Napoca (RO)

Project website:

Abstract:

The aim of this project is to obtain supramolecular nanomaterials functionalized with chromophores and fluorescent compounds and nanostructured platform for the development of biosensors with possible applications in the detection of biomedical and pharmaceutical compounds. For this purpose new nanostructured surfaces with photoelectrochemical properties will be develop by combining chromophores or fluorophores with new nanomaterials. Several molecules are taken into account: photosensitive transition metal complexes such as complexes of Ru(II) with pyridine having photoelectrochemical and electrochemiluminescence properties, and tetrazine derivatives, due to their fluorescent properties. Another objective of the project is to obtain nanostructured platforms by nanospheric lithography for the controlled immobilization of biomolecules and rational design of biosensors, with possible applications in biomedical analysis. Modified electrodes with graphene, cyclodextrins and polymer films for detection and simultaneous determination of ascorbic acid, uric acid and dopamine in pharmaceutical and biological samples will also develop. This collaboration will enable the development of new research directions on the topic of biosensors using the scientific skills of each team.

FILE DESCRIPTION	DOCUMENT
List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
R&D activities in enterprises
Peer-review activity for international programs/projects