BrainMap

Sorina Dinescu

- UNIVERSITATEA BUCURESTI

Researcher | Teaching staff | Scientific reviewer

Web of Science ResearcherID: https://www.webofscience.com/wos/author/record/H-1410-2016

Expertise & keywords

The landscape and management effects on clonal variation and microbial symbiont diversity of the corn leaf aphid (Rhopalosiphum maidis Fitch) Call name: P 4 - Proiecte de cercetare exploratorie - PCE-2021
PN-III-P4-PCE-2021-0543 2022 - 2024

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA SAPIENTIA

Project partners: UNIVERSITATEA SAPIENTIA (RO)

Affiliation: UNIVERSITATEA SAPIENTIA (RO)

Project website: https://sites.google.com/view/aphidsymbionts/f%C5%91oldal

Abstract:

Over the past 10 years, damages in agriculture increased significantly because of aphids, having an annual amount of 50-milliard euro worldwide. One of the most important pest is the corn-leaf aphid (Rhopalosiphum maidis). Aphids are also closely associated with microorganisms, for instance the obligate mutualist endobacterium (usually referred to as a primary symbiont), Buchnera aphidicola, which is maternally inherited. In addition, other maternally transmitted intracellular bacteria, such as Rickettsia sp. (α-Proteobacteria), Spiroplasma spp. and various γ-proteobacterial microbes (including Hamiltonella defensa, Regiella insecticola, Serratia symbiotica and Arsenophonus sp.) are harboured by aphids. Given that secondary symbionts play different roles in aphid adaptation and evolution, an important questions remains: O1: Can host plant (maize) management systems and its landscape complexity influence the corn-leaf aphid microbial (symbionts) diversity? O2: Are these taxonomic compositions of bacterial symbionts stable within the aphid populations and clones during years? O3: Are these bacterial diversity variation related to particular maize management systems, meaning that aphid clonal variations and pest control used are associated with particular secondary symbionts?

Improvement of original antitumor compounds pharmacological parameters using targeted nanoshuttles designed for colorectal cancer modern approach Call name: P 1 - SP 1.1 - Proiecte de cercetare pentru stimularea tinerelor echipe independente - TE-2021
PN-III-P1-1.1-TE-2021-1660 2022 - 2024

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://scienceonlinero.wixsite.com/research/104teoncotarget

Abstract:

Recent statistics rank colorectal cancer (CRC) as the second cause of cancer-related deaths and third as incidence and highlight the emerging need to improve the currently available therapeutic approaches. In the context of increasing resistance and dose-limiting side effects to traditional chemotherapy, there is a real need for the development of novel therapies, thus improving the current clinical landscape and increase patient’s survival chances. However, despite identifying novel potent anticancer agents, these exhibit inappropriate pharmacological parameters for subsequent administration. In this context, the present proposal aims to fine-tune the pharmacological profile of novel lanthanide complexes of quinolone derivatives (oxolinic acid, nalidixic acid, and difloxacin) by their entrapment in original HA-poly (DMAEMA) targeted drug-delivery systems approaching colorectal cancer management. More, the current proposal fits within the modern direction of drug repositioning by bringing the activity of ‘older’ drugs back into clinical use for purposes that are outside the scope of the original medical indication.

Personalized dressing platform based on smart materials for wound management Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-1776 2022 - 2024

Role in this project: Key expert

Coordinating institution: INSTITUTUL DE CHIMIE ORGANICĂ ŞI SUPRAMOLECULARĂ "COSTIN D.NENIŢESCU"

Project partners: INSTITUTUL DE CHIMIE ORGANICĂ ŞI SUPRAMOLECULARĂ "COSTIN D.NENIŢESCU" (RO); UNIVERSITATEA NAŢIONALĂ DE ŞTIINŢĂ ŞI TEHNOLOGIE POLITEHNICA BUCUREŞTI (RO)

Affiliation: INSTITUTUL DE CHIMIE ORGANICĂ ŞI SUPRAMOLECULARĂ "COSTIN D.NENIŢESCU" (RO)

Project website: https://www.icoscdn.ro/index.php/proiecte/dresmater

Abstract:

The present project aims at developing a personalized smart platform for wound diagnosis and treatment based on biohybrid nanofibers nonwoven fabrics composed of functionalized poly(2-isopropenyl-2-oxazoline) (PiPOx), gelatin, and active therapeutic agents. PiPOx emerged as a novel and versatile platform to develop advanced functional materials, showing high potential to be used in the development of biomaterials. The versatility of PiPOx polymer consists in its hydrophilic and biocompatible character, and in the fact that it can be modified to simultaneously introduce multiple and challenging functional handles, by ring opening addition reactions in the presence of various reactive groups. This advantage allows the chemical connection between the synthetic layer, functionalized PiPOx, with the biopolymer layer, gelatin, to tackle the problems related to the mechanical and hydrolytic stability of the resulted biohybrid nanofibers. Via the herein proposed objectives and activities, the multidisciplinary team of specialists undertook the challenge to demonstrate that functionalized PiPOx-gelatin based-nanofibers can be used as dressings for in situ visual detection of bacterial infection by continuously monitoring the pH, as well as for administration of therapeutic molecules on-demand, possessing intrinsic antibacterial properties and promoting wound healing. Based on the previous findings on PiPOx polymer and the expertise of the team in material design, electrospinning, and in vitro testing, the technology readiness level of the proposal starts at 2, and ends at 4, with the proof-of-concept.

Design, modeling and validation of novel biohybrid for wound healing applications by tissue engineering Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-2917 2022 - 2024

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU TEXTILE SI PIELARIE-I.N.C.D.T.P. BUCURESTI SUCURSALA BUCURESTI INSTITUTUL DE CERCETARE PIELARIE - INCALTAMINTE I.C.P.I. (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://sorinadinescu.wixsite.com/622pedhealskin

Abstract:

Chronic wounds affect patients’ life quality, as they require repetitive treatments and incur considerable medical costs. Some are incredibly dangerous – e.g. diabetic ulcers, which precede 85% of amputations, while some can take decades to heal, thus contributing to secondary conditions such as depression. Thus, much effort has been focused on developing novel therapeutic approaches for these types of wounds. In this regard, an improved method of treatment would be the topical application of therapeutic agents with multiple properties (anti-inflammatory, antioxidant, antimicrobial, pro-angiogenic, pro-regenerative) that could facilitate the impaired healing process of such lesions. In this context, the aim of HEALSKIN project is to develop and validate at lab scale an innovative biohybrid with multiple therapeutic properties for chronic wound healing applications, based on natural components (collagen, sericin) and improved with two flavonoids (curcumin and quercetin). We plan to develop and evaluate the biocompatibility of HEALSKIN, cellular adhesion and cytoskeleton development, anti-inflammatory effect and pro-regenerative potential on normal skin cells (fibroblasts/keratinocytes), and investigate the efficiency of HEALSKIN in simulated oxidative stress conditions, as well as test its pro-angiogenic potential on endothelial cells and its antimicrobial effect on several bacterial strains. By the end of the project we expect to obtain a biocompatible material, capable of promoting cell viability, adhesion and proliferation, combat excess inflammation, oxidative stress and bacterial infection, favor regeneration and angiogenesis, in order to validate HEALSKIN as a potential candidate for skin regeneration. HEALSKIN could have an extraordinary impact on the improvement of therapeutic possibilities, contributing to faster recovery, better emotional outcome, and in general an increase in life quality level for the patients.

Molecular mechanisms involved in biomaterial-assisted peripheral nerve regeneration based on mesenchymal stem cells Call name: P 1 - SP 1.1 - Proiecte de cercetare pentru stimularea tinerelor echipe independente
PN-III-P1-1.1-TE-2019-1191 2021 - 2022

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://sorinadinescu.wixsite.com/197temagnificent

Abstract:

Injuries to the peripheral nervous system (PNS) have been listed as a serious affections among the patients. Any kind of trauma to the PNS can affect the quality of a patients’ life by loss of motor and sensory functions, essential for a normal lifestyle. Therefore, the focus of this research project is to investigate a new and modern approach of tissue engineering for peripheral nerve regeneration (PNR). In this respect, the general aim is to evaluate human adipose-derived stem cells’ (hASCs) potential for PNR assisted by novel gelatin (G) electrospun materials containing magnetic nanoparticles (M) and carbon-derived nanospecies (G) [GMG]. The objectives to achieve this aim are: (O1) to evaluate GMG biocompatibility with hASCs, neural stem cells and Schwann cells; (O2) to assess GMG potential to support hASCs differentiation towards neuron-like and Schwann-like cells; (O3) to investigate M and magnetic field potential for hASCs-mediated PNR; (O4) Investigation of carbon derivatives influence on hASCs-mediated PNR. The outcomes of the present research proposal will consist in the validation of a novel biomaterial to assist PNR, which at the same time will be personalizable, due to the use of hASCs. Moreover, for the first time, the simultaneously incorporation of graphene oxide and magnetic nanoparticles will be investigated in the event of PNR. Another key result of this project will be to elucidate the effect of MNPs and applied magnetic field on hASCs differentiation towards neuron/glial cells in correlation with epigenetic modifications. Answers regarding mitochondrial remodeling and signaling pathways in the context of PNR will be also be provided at the end of the project. All these results will not only deliver new insights intro PNR, but will also bring a significant contribution to the scientific field and have an impact at social and economic levels, through offering a customizable treatment.

Nano-engineered hydrogels for 3D bioprinted bone scaffolds Call name: P 1 - SP 1.1 - Proiecte de cercetare pentru stimularea tinerelor echipe independente
PN-III-P1-1.1-TE-2019-0787 2020 - 2022

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Affiliation: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Project website: https://boneposs.wixsite.com/boneposs

Abstract:

Proiectul propune realizarea unor materiale bioinspirate si nano-structurate cu proprietati intrinseci si extrinseci semnificativ imbunatatite pentru a genera hidrogeluri nanocompozite de interes pentru ingineria tisulara osoasa. Proiectul exploreaza o combinatie fascinanta de doua tipuri de compusi nanostructurati diferiti integrati intr-o matrice polimerica hidrofila: compusi nano-structurati cu structura organic-anorganic de tip cusca (POSS) si compusi nanostructurati naturali sub forma de nanofibre (nanoceluloza). Matricea organica in care vor fi incorporati acesti nano-aditivi reactivi va fi deasemenea o combinatie special proiectata de polimeri biocompatibili. Se vor utiliza polimeri naturali precum proteine (ex. derivati de gelatina) si/sau polizaharide (alginat/ pectina) pentru a simula matricea extracelulara nativa a celulelor. Pentru a explora cele mai noi tehnici de generare a constructelor tisulare destinate ingineriei biomedicale, in proiect se va folosi o tehnologie performanta de fabricare aditiva (printarea 3D). Tehnica printarii 3D va fi utilizata pentru a genera arhitecturi complexe perfect controlate folosind formularile nanostructurate dezvoltate in proiect ("nano-inks").

Proiectul continua intr-o maniera extrem de provocatoare dorind sa evolueze de la abordarea printare 3D ce foloseste hidrogelurile nanostructurate printate pentru cultivarea de celule la abordarea BIOprintare 3D prin incorporarea de celule direct in materialele precursor ("nano-bioinks"). In plus sunt prevazute in proiect experimente in vivo (testare pe animale) pentru a integra in materialele printate 3D si a patra dimensiune (timpul).

Astfel formularile polimerice nanostructurate dezvoltate in proiect vor fi complet studiate si ar putea deveni materiale cruciale ce permit reconstructia individualizata a tesuturilor semi-dure lezate.

New gemmotherapy-based Coryllus avelana reinforced with chrysin/cyclodextrin nanocomplexes designed for liver fibrosis in diabetes mellitus Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-3609 2020 - 2022

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA DE VEST "VASILE GOLDIŞ" ARAD (RO); PLANTEXTRAKT S.R.L. (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte-de-cercetare/proiecte-cu-finantare-nationala/pn-iii-p2-2-1-ped-2019-3609-262ped/

Abstract:

Diabetes and liver fibrosis are two chronic diseases, but the co-existence of the two pathologies is certainly much more debilitating than each of these entities independently, with a significant impact on the quality of life. In this project, we aimed to develop a novel formulation of Coryllus avelana gemmotherapic extract reinforced with chrysin, based on the nanoformulations of chrysin-cyclodextrin complexes (CAG-CHR/Cyclo). Based on our preliminary results, we start the project from TRL2. In order to achieve TRL4, we will accomplish the following objectives: (1) in the first stage we aimed to obtain the newly CAG-CHR/Cyclo. We will develop a two-step method for the formulation of this composite system, at first the nanoformulation of chrysin, then the additive process of the extract. The nanofromulation will be made by the complexation of chrysin by hydroxypropyl-beta cyclodextrin (HPBCD) and randomly methylated beta cyclodextrin (RAMEB). The characterization of the complex and the complex+extract system will be characterized by analytical methods; (2). in the second stage the products obtained previously, will be tested for anti-oxidant and anti-fibrotic potential by in vitro experiments made by UB; (3). in the third stage, the best ranked gemmotherapy-nanocomplex will be validated for the anti-fibrotic potential on CCl4-induced liver fibrosis in diabetic mice, which confirmes the level of TRL4 at the end of the project.

Selection and dissemination of antibiotic resistance genes from wastewater treatment plants into the aquatic environment and clinical reservoirs Call name: P 4 - Proiecte Complexe de Cercetare de Frontieră
PN-III-P4-ID-PCCF-2016-0114 2018 - 2022

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE -DEZVOLTARE PENTRU ECOLOGIE INDUSTRIALA - ECOIND (RO); INSTITUTUL NATIONAL DE BOLI INFECTIOASE ''PROF.DR.MATEI BALS'' (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE MEDICO-MILITARA „CANTACUZINO” (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://bios.unibuc.ro/Proiecte/pn-iii-p4-id-pccf-2016-0114.html

Abstract:

The RADAR project aims to assess the prevalence and dissemination of antibiotic resistance (AR) from urban, clinical and industrial wastewater into the aquatic environment via wastewater treatment plants (WWTPs). Moreover, RADAR will give information on the environmental and clinical resistome, identifying the possible mechanisms of AR emergence and spread.

Scientific objectives: i) investigation of WTTPs resistome to establish the prevalence of AR bacteria (ARB)/genes (ARGs) in the environmental samples from upstream-WWTP-downstream transects; ii) evaluation of selected antibiotics (beta-lactams, fluoroquinolones and macrolides) occurrence in both WWTP’ influent and effluent; iii) comparative analysis of geographically and time related ARB/ARGs from wastewaters and clinical sources; iv) assessing and prediction how the presence/absence of ARGs and their relative abundance depending on a class of geographical, hydrological, physico-chemical and microbiological factors.

Methodology: The urban, clinical, farming and industrial wastewater will be monitored by physico-chemical, microbiological methods and metagenomics throughout various steps, i.e.: 1000 m upstream river, influent, different treatment steps inside WWTP, effluent and 200 m downstream river. Wild fish from the receiving river after discharge of WWTP effluent will be also analyzed. The following analyses will be performed: i) LC/MS and HPLC techniques to monitor the levels of selected antibiotics; ii) isolation and identification of ARB belonging to ESKAPE species; iii) evaluation of metagenomic resistome by bioinformatic data processing; iv) environmental and clinical ARGs and plasmids sequencing; v) analyzing the potential of ARGs for transferability and environmental/clinical risk by mapping the insertion loci of transposable elements and the ARGs potential to mobilize the resistance genes in a bioreactor model; vi) elaboration of a prediction model for the occurrence of ARB/ARGs

Liquid biopsy - a modern alternative in tumor molecular profiling by NGS analysis for therapy modultion in advanced stages of cancer. Validation and implementation. Call name: P 2 - SP 2.1 - Proiect de transfer la operatorul economic
PN-III-P2-2.1-PTE-2019-0577 2020 - 2022

Role in this project: Key expert

Coordinating institution: ONCO TEAM DIAGNOSTIC S.R.L.

Project partners: ONCO TEAM DIAGNOSTIC S.R.L. (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://scienceonlinero.wixsite.com/research/29pte-oncongs

Abstract:

Considering that one of the main purposes of the current call is to link applied research and technological progress in Romania to the evolution and current requirements of the national and international socio-economic environment, the present proposal aims to change the clinical practice in Romania by validating and placing on the national market of a novel non-invasive method for prognostic and therapy modulation for patients diagnosed with advanced cancer. The novel laboratory service described in the present grant proposal is approaches liquid biopsy for molecular profiling of tumors through next generation sequencing (NGS) technique in order to modulate therapy in advanced lung and colorectal cancer. The liquid biopsy overcomes the limitations identified in classic tissue biopsy, being a non – invasive technique that can be easily repeated as needed, therefore providing real time information on tumor dynamics after circulating biomarker analysis (circulating tumor cells, circulating tumor DNA, exosomes). The major advantage of circulating tumor DNA using NGS lies in the complexity of the data provided by an ultra – modern technology that allows screening of multiple molecular alterations, in a one – time analysis of the same sample. The results obtained after data analysis of NGS results will make a huge impact on advanced cancer patients treatment, offering the possibility of real – time treatment adjustment based on the dynamics of the identified molecular changes. The implementation of this project in Romania could offer the opportunity of romanian patients to benefit from circulating tumor DNA analysis through NGS without the risks associated with overseas shipment of extremely perishable biological samples and with significant lower costs. The innovative character of the present grant proposal consists in developing a unique panel of target genes relevant for both lung and colorectal cancer.

The development in oncology of novel radiopharmaceuticals and nuclear techniques for diagnostic imaging and personalized treatment at molecular level Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0769 2018 - 2021

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA SI INGINERIE NUCLEARA " HORIA HULUBEI " - IFIN - HH

Project partners: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA SI INGINERIE NUCLEARA " HORIA HULUBEI " - IFIN - HH (RO); INSTITUTUL DE BIOCHIMIE (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE MEDICO-MILITARA „CANTACUZINO” (RO); UNIVERSITATEA BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE IN DOMENIUL PATOLOGIEI SI STIINTELOR BIOMEDICALE "VICTOR BABES" (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU TEHNOLOGII IZOTOPICE SI MOLECULARE I N C D T I M (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE "CAROL DAVILA" (RO); INSTITUTUL CLINIC FUNDENI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://www.nipne.ro/proiecte/pn3/9-proiecte.html

Abstract:

The ONCORAD project and the associated technological platform in Radiobiology, is built on the concepts of translational medicine and has as main objective the development of innovative targeted radiopharmaceuticals and nuclear medicine techniques for diagnostic imaging and improved radiotherapy in cancer. ONCORAD is based on the unique expertise of the coordinator (IFIN Horia Hulubei, Magurele) ) in the field of radioisotopes and radiopharmaceuticals, complemented by the exquisite know how of partner institutions in the field of biomedical research (INCD Victor Babes, Cantacuzino National Research Institute and Biochemistry Institute, Bucharest) and clinics (Fundeni Clinical Institute and Colentina Clinical Hospital, Bucharest), along with the expertise of INCD for Isotopic and molecular Techniques, Cluj-Napoca in nanotechnologies/nanomedicine. The multidisciplinary effort is focused in 4 interconnected research projects in the field of oncology and nuclear medicine. Additionally, the ONCORAD project will sustain the development of the partner institutions by supporting new jobs for young researchers and their extensive training, infrastructure development, and transfer of knowhow, technologies and good laboratory practices among partners and towards interested third parties. ONCORAD will build an organizational structure for enhanced interdisciplinary collaboration in the field of radiobiology, in the benefit of research, patients and oncologists. The project is a premise for future participation of the consortium in large-scale projects and in European networks/platforms in the field of nuclear medicine and nanomedicine, enlarge the research services and technological transfer for clinical applications.

Advanced Innovative approaches for predictable regenerative medicine Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0782 2018 - 2021

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE IN DOMENIUL PATOLOGIEI SI STIINTELOR BIOMEDICALE "VICTOR BABES" (RO); INSTITUTUL ONCOLOGIC PROF.DR.I.CHIRICUTA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte-de-cercetare/proiecte-cu-finantare-nationala/65pccdi-2018/

Abstract:

Considering that HEALTH is one of the main areas of public priority, the approach of interdisciplinary researches with applicability in Regenerative Medicine can significantly contribute to improving the quality of life of patients with tissue defects. The aim of the project is to create a consortium with complementary research experience in the field of regenerative medicine, which will efficiently use the human resource and modern research infrastructures newly created for the implementation of innovative technologies, with the aim of developing and transferring the results to the economic environment. The project aims to develop new technological products in the form of biocompatible biomaterials designed for bone reconstruction (P1), nerves (P2), soft tissues (P3) and breast reconstruction after tumor resection (P4).

Integrated development project for advanced medical treatment technologies Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0728 2018 - 2021

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE MEDICO-MILITARA „CANTACUZINO” (RO); INSTITUTUL DE BIOCHIMIE (RO); UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA PITESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://teramed.inflpr.ro/

Abstract:

In order to increase community’s quality of life, the aim of the project proposal entitled “Integrated development project for advanced medical treatment technologies” (TERAMED) is to develop novel technologies with respect to the treatment of osseous and cutaneous conditions and oncological disorders. Given our experience in healthcare research and the current requirements of multidisciplinary and interinstitutional collaboration towards the personalized treatment purpose, the TERAMED project aims genuine synthesis and processing of biomaterials, but also functional and therapeutic evaluation relevant for clinical trials. The main objectives of the “Medical devices functionalized by laser technologies and alternatives for enhanced osseous integration and regeneration” subproject are to design and produce inorganic, composite or hybrid coatings for superior osteoconductive and osteoinductive performances of titanium-based implants. Smart wound patches and polymeric gels functionalized with antimicrobial and wound healing biomolecules incorporated within micro- and nanoparticles constitute the purpose of the “Medical devices (patches and gels) based on composite biomaterials obtained by laser, plasma and radiation technologies and alternatives for enhanced healing of cutaneous injuries” subproject. The “Technologies based on magnetic triggered nanostructures for oncological therapy: early diagnosis and targeted treatment” subproject aims the development of multifunctional medical devices for specific and selective diagnosis and treatment of breast cancer and melanoma. The general impact of the TERAMED project ensues from the beneficial conjunction of the clinical potential of the proposed medical devices, the feasible technological transfer and the economic advantages of interinstitutional collaboration.

Smart materials for medical applications Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0407 2018 - 2021

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); CENTRUL DE CHIMIE ORGANICA AL ACADEMIEI ROMANE "C.D.NENITESCU" (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE "CAROL DAVILA" (RO); UNIVERSITATEA BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE CHIMICO - FARMACEUTICA - I.C.C.F. BUCURESTI (RO); Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE "GRIGORE T. POPA" DIN IAŞI (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://intelmatupb.wixsite.com/intelmat

Abstract:

INTELMAT project represents advanced research in the field of synthesis and application of smart materials for medical engineering in order to solve essential features of some acute/chronic diseases of large occurrence. 5 thematic directions are taking into consideration: 1. Developing of a controlled-release system of complex micro-colloidal architectures based on bacterial cellulose and hydrogels used for management of chronically wounds which aims to overcome the limitations of classical treatments; 2. Developing of new biomaterials specially designed with targeted action for treatment of inflammatory diseases of gastrointestinal segment; 3. Synthesis of new generation of composite membrane materials for artificial kidneys based on biocompatible polymers and derivative graphene; 4. Developing of some auto-assembly 3D platforms with controlled-released drugs based on polymeric nanoparticles and composite nanogels for the therapy of colon-rectal cancer; 5. Developing of innovative technologies for the synthesis of some 1D nano-architectures (nanowires) with controlled morphology, with applications in producing of non-enzimatic electrochemical biosensors.

Multi-disciplinary platform for regional institutional capacity enhancing in dermatooncology and dermato-pathology domains Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0341 2018 - 2021

Role in this project: Key expert

Coordinating institution: SPITALUL CLINIC "COLENTINA" BUCURESTI

Project partners: SPITALUL CLINIC "COLENTINA" BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE IN DOMENIUL PATOLOGIEI SI STIINTELOR BIOMEDICALE "VICTOR BABES" (RO); INSTITUTUL DE BIOCHIMIE (RO); UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE "IULIU HATIEGANU" (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE "CAROL DAVILA" (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://cdpcolentina.ro/buton11pathderm.html

Abstract:

Dermato-oncology and dermato-pathology address the most frequent pathology, but the medical training is scarce, hence the new diagnosis and treatment approaches in skin cancers are frugaly tought in residency and in post-universitary training. Thus, the medical act is hindered by the “in-house” training of the clinicians that can lead to low quality medical serivces for the patient. The project re-unites 6 prestigious institutes that have prior collaborated (Bucharest, Cluj): an university hospital, a national institute, a Romanian Academy national institute and 3 universities (two medical ones) that propose for the first time on national level a research and training platform for dermato-oncology and dermato-pathology developing 4 multi-disciplinary projects. Project 1 developps an improved diagnostic and prognostic markers for the most frequent skin cancers- squamous cell and basal cell carcinomas. Project 2 identify the markers for improved prognosis and therapy monitoring in cutaneous melanoma. Project 3 develops new methologies for therapy testing in dermato-oncology and dermato-pathology. Project 4 completes the training slope of young researchers and medical doctors to increase the medical services quality (decrease social costs of the dermato-oncology pathology) and to increase the research results in the biomedical domain. We estimate a significant impact of the results on the institutional capacity consolidation regarding human resources (13 new research positions, several training stages, several work visits) and infrastructure (upgrading of the existent infrastructure); 3 patents, 3 new sets of biomarkers for diagnostic/prognostic/monitoring for the increase of the health care system, RD services, and international visibility improvement.

From the classical nutrition to precise nutrition in animal production, scientific basis for the nutrition security of the population Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0473 2018 - 2021

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA - IBNA BALOTESTI

Project partners: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA - IBNA BALOTESTI (RO); UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA BABES BOLYAI (RO); INSTITUTUL DE BIOCHIMIE (RO); UNIVERSITATEA "ŞTEFAN CEL MARE" DIN SUCEAVA (RO); UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.ibna.ro/proiecte-de-cercetare/item/110-pn-iii-p1-1-2-pccdi-2017-0473

Abstract:

The overall objective of the project is to strengthen the institutional capacity of IBNA by developing new research directions based on „omics” technologies – nutrigenomics, toxicogenomics, proteomics, metabolomics – and to relaunch the Laboratory of Biotechnology. This will generate a set of innovative outcomes in animal nutrition. The innovative aspects of the project include: use of agro-food wastes as adsorption and detoxifying agents for feed contaminants; testing the effect of phytoadditives (plants or plant extracts not yet used for such purposes) as antibiotic replacers (willow bark extracts) on the interaction between the intestinal tract physiology and the intestinal microflora; investigation of several less characterized and used protein-oleaginous sources (cowpea, Jerusalem artichoke, etc.); in vitro experimental models to evaluate the decontaminating potential of wastes under conditions of concurrent fungal, viral and microbial contamination; development of new feeding products which ensure a more efficient use of the protein in ruminants.

The project covers three regions of development and the partners are prestigious institutions, with complementary infrastructure and expertise, with converging scientific interests. The consolidation of new directions will enhance, both for IBNA (the relaunched institution), and for its partners, their capacity of applying for/running research projects, allowing studies with a high level of complexity, which generate practical results, relevant for the development of the animal production sector.

The project will impact on the human resources, the budget allowing the employment of 14 researchers (of which 10 by IBNA), whose specialisation is already ensured; it will also support the enhancement of the scientific expertise of the senior researchers by using vouchers. The training periods will enrich the portfolio of methods and techniques that can be applied for the directions in which IBNA aims to develop/relaunch.

Molecular investigation of the exosomes-mediated interaction between stem and tumor cells Call name: P 1 - SP 1.1 - Proiecte de cercetare Postdoctorală
PN-III-P1-1.1-PD-2016-2057 2018 - 2020

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://pd2016.wixsite.com/46pdmiami

Abstract:

Human adipose-derived stem cells (hASCs) represent adult mesenchymal stem cells with beneficial properties for tissue engineering (TE) and wound healing, thus being currently considered reliable resources for implantation in regenerative medicine purposes. However, a number of studies showed that (1) hASCs possess the ability to respond to cancer signals and to migrate from the adipose tissue towards a tumor site and that (2) they are susceptible to genomic instability and neoplastic transformation. Exosomes are currently considered nanoshuttles of RNAs and proteins that facilitate communication between cells.

Thus, the driving hypothesis of the present project proposal is that communication between hASCs and breast cancer cells (BCCs) in a tumor microenvironment via exosomes can possibly lead to a different response of hASCs during a regeneration/wound healing process by alteration of gene expression and signaling in these stem cells.

The general aim of this research proposal is to investigate the in vitro exosome-mediated intercellular communication between hASCs and BCCs, with the purpose of testing hASCs safety for use in stem cell –based therapies. The specific objectives are: (O1) to investigate hASCs-derived exosomes and their miRNAs; (O2) to investigate BCCs-derived exosomal miRNAs function on gene expression and epigenetic mechanisms in hASCs recipient cells and to (O3) screen the non-coding RNAs cargo in the exosomes released by hASCs post-interaction with BCCs.

Project impact envisages the scientific and medical fields- by improving knowledge in cancer research, TE, exosome-mediated communication and ethical aspects of using stem cells. The project results directly address patients with tissue defects or disease that are recommended stem-cell based therapies.

Expected results include identification of miRNAs function in hASCs-BCCs interaction and a molecular-based conclusion on the safety of hASCs use for stem cell based therapies.

Establishing nanomaterial grouping/ classification strategies according to toxicity and biological effects for supporting risk assessment Call name: P 3 - SP 3.2 - Proiecte ERA.NET
ERA-SIINN-NanoToxClass 2015 - 2019

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://www.unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte-de-cercetare/proiecte-cu-finantare-nationala/13-siinn-2015/

Abstract:

Currently, risk assessment of manufactured nanomaterials (MNM) is done on a case-by-case basis.Given the broad range of different MNM including all possible variations such as size, shape or coating this is a laborious, time- and cost- intensive process. Testing all MNM variants for all possible endpoints is not feasible. One solution to overcome this problem is grouping of MNM. Here we will focus on grouping according to toxicity and biological effects in humans and mammals. We will establish criteria for grouping on the basis of well-established toxicity endpoints, and include novel approaches such as transcriptomics, proteomics and metabolomics. We will consider existing data from finished projects and published studies. Experimental work in the project is planned in a targeted,selective manner, mainly focussing on high content or Omics data delivering information on modes of action. Validation of grouping will be carried out for a few selected MNM.

Design and development of cyclodextrin-based delivery systems to enhance chrysin antifibrotic activity in chronic liver diseases Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-1644 2017 - 2018

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE VEST "VASILE GOLDIŞ" ARAD

Project partners: UNIVERSITATEA DE VEST "VASILE GOLDIŞ" ARAD (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.uvvg.ro/antifibrosis/

Abstract:

Epidemiological data indicate that approximately 180 millions of patients worldwide are affected by a form of chronic liver diseases that can be induced by a number of well defined etiological agents. Among diseases of the gastro-intestinal tract, cirrhosis represents the 7th most common cause of death in western countries. The process of liver fibrosis can be reversible, whereas cirrhosis, the end-stage consequence of fibrosis, is generally irreversible.

The project aim is to develop, test and validate a new drug delivery system of chrysin based on cyclodextrins targeted liver fibrosis.

Objectives:Development and characterization of chrysin/cyclodextrin complexes; Evaluation of in vitro biocompatibility and intestinal absorption for chrysin/ cyclodextrin complexes;Validation of antifibrotic effects of chrysin/cyclodextrin complexes in animal liver fibrosis model.Chrysin have limited water solubility, poor oral bioavailability, and can be easily modified by environmental factors such as temperature, pH and light. Water-soluble hydroxypropyl (HPBCD),sulfobutylether (SBE) and randolmy methylated (RAMEB) β-cyclodextrins compared with parent β-cyclodextrin (BCD) will be used to enhance antifibrotic properties of chrysin, by increasing the solubilization potential and prevention of metabolic degradation within the gastrointestinal tract. The solubility and in vitro intestinal permeability will be compared and the four formulations will be ranked. Thus, we expected to select the first two best inclusion complexes. In the finally step, after in vivo experiments, we will validate one single chrysin-cyclodextrin complex with high potential to treat liver fibrosis and antifibrotic mechanism will be established.

At the beginning of the project the TRL value is TRL3. After in vitro and in vivo tests and analysis, will be validate one single chrysin-cyclodextrin complex with highly potential to reverse liver fibrosis, which confirmes the level TRL4 at the end of the project.

Transfer of knowledge regarding optimal use of platelet-rich plasma (PRP) for efficient tissue regeneration Call name: P 2 - SP 2.1 - Transfer de cunoaștere la agentul economic „Bridge Grant”
PN-III-P2-2.1-BG-2016-0458 2016 - 2018

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); PROESTETICA HOSPITAL SRL (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://123bridgegrnt.wixsite.com/expert

Abstract:

Currently, plasma rich in platelets (PRP)-therapy is a modern, non invasive bioregenerative treatment and one of the newest procedures used in regenerative medicine and plastic surgery for tissue lesions, wound healing, deep burns or cartilage injuries. The Challenge (request) formulated by Proestetica Hospital Srl is the need to adapt GloFinn modern technology currently used in this clinic to obtain PRP to the age of each patient, since variation in patient-response to PRP-therapy occurs. The AIM of this project is to optimize the use of autologous PRP fraction depending on the patient's age by means of research activity (University of Bucharest) and to transfer this Knowledge to the medical environment in order to solve the defined Challenge. The objectives of this proposal are: (O1). Evaluation of the concentrations of growth factors in PRP fractions isolated from patients, depending on age; (O2). Investigation of the effect of PRP growth factors levels (identified in O1) on the regenerative process at cellular level; (O3). Achievement of an inter-relation between the level of PRP isolated depending on patient age and the levels of PRP necessary for an efficient regeneration process, followed by transfer of knowledge from research to clinic. Long-term impact of this study targets a more efficient use of PRP in healing processes and regeneration, having tremendous impact in medical and social environment: healing wounds, deep burns, wounds that do not close, regeneration of cartilage lesions, joints, etc., thus contributing to the evolution of knowledge in the field of tissue engineering and regenerative medicine.

Liquid biopsy technology development for prognostics and therapy modulation in malignant epithelial cancers Call name: P 2 - SP 2.1 - Proiect de transfer la operatorul economic
PN-III-P2-2.1-PTE-2016-0149 2016 - 2018

Role in this project: Key expert

Coordinating institution: ONCO TEAM DIAGNOSTIC S.R.L.

Project partners: ONCO TEAM DIAGNOSTIC S.R.L. (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.tumflow.wixsite.com/tumflow

Abstract:

The goal of this project application is to change the clinical practice in Romania by validating and putting on the internal medical market a novel noninvasive method for prognosis and therapy modulation in cancer.

Early in the development and growth of a primary tumor (breast, colon, lung, prostate), some of the cells detach and spread in the blood circulation (106 cells/1 g tumor tissue/day). These cells circulate in the peripheral blood of patients (1-10 cells/1ml peripheral blood) alone or clustered. Analyzing these circulating cells derived from previously diagnosed tumors can be defined as a real time liquid biopsy, capable of offering crucial information for prognosis and therapy modulation. The technology we propose in this project for using liquid biopsies consists in the detection and counting of the circulating tumor cells by flow cytometry in peripheral blood from patients diagnosed with epithelial tumors. This method will improve the evolution tracking process and will increase the therapy efficiency in Romania.

The urgent necessity for implementing this project proposal is due to the limited availability of this type of analysis for Romanian patients, this analysis being available at the moment only in USA and a few UE states, where the access for Romanian patients is hampered by high costs involving the sample transport and the analysis itself (600 euro), and also by the lack of information. Other major inconveniences are the risk of damaging the sample during transportation and the occurrence of false results due to the long period of time between sampling and the actual analysis. Thus, the most majority of the oncologic patients in Romania are deprived from the opportunity of monitoring their disease evolution through a simple and noninvasive method that can offer them an accurate prognostic and the possibility for therapy modulation, significantly increasing the survival rate.

Improving oral and systemic health using dental works from modified alloys Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0869 2014 - 2017

Role in this project: Key expert

Coordinating institution: UNIV.DE MEDICINA SI FARMACIE - CAROL DAVILA

Project partners: UNIV.DE MEDICINA SI FARMACIE - CAROL DAVILA (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL DE CHIMIE FIZICA - ILIE MURGULESCU (RO); UNIVERSITATEA BUCURESTI (RO); R&D CONSULTANTA SI SERVICII S.R.L. (RO); DENTAL ART GROUP SRL (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://cercetare-umf.ro/proiecte_parteneriate

Abstract:

The project proposal entitled “Improving oral and systemic health using dental restorations from modified alloys” is addressed to the priority field 4 Health and has as goal the improvement of oral and systemic health of the population through implementing new methods of prevention and intervention at national level having capacity of international expansion. Having the central objective the improvement of the health level mainly through the offer of medical services of prevention, the project will contribute to the reduction of costs in the field of health. The offer is elaborated under the competent and representative direction of the University of Medicine and Pharmacy “Carol Davila”, the Faculty of Dental Medicine, another 2 Universities, 1 Institute of research of the Romanian Academy and 2 co-financing SMEs. This consortium is by definition inter- and pluri-disciplinary and exhibits synergy and complementarity due to the contribution of Medicine, Chemistry and Biology to the achievement of the central objective and it shall benefit of the un-financed support of an European partner (Universita’ del Piemonte Orientale “Amedeo Avogadro”) well known in the field of oral pathology, partner which shall participate to the activity of research and dissemination of results. This partner with experience and tradition will enhance the chances of the project both at level of the choice of the best methods of prevention and intervention in oral health and also at the level growing the visibility of the research. As derived objectives to achieve the central goal, the project takes into consideration the formation of target groups of patients according to the following criteria of selection: the detection of lichenoid reactions, of a metallic pigmentary lesions; the existence of dental alloys (CoCr, CoCrMo, etc. used at mass level) which favoured the release of ions and the absorption due to materials imperfections. The project will also form proof-lots including in the study a high number of patients comprising also patients who did not use dental alloys, fact which will allow the set out of the cause of the diseases and of the most appropriate treatments for all the studied cases. Both the scientific background and the expertise of the 6 partners as well as the richness of human and material resources represent a warranty that it is possible to overlap the knowledge about the creation of an improved alloy (CoCrZr or Nb) capable to reduce the potential for serious diseases such as Lichen Planus. The project analyses the sick tissue and the amount of ions released chemically and biologically and to compare the behaviour of the existing materials with that of our new alloy. The improvement of the dental alloy will aim at both the composition as well as the surface state at micro- and nano- level. The trial of these materials will be performed in simulating conditions respecting the corresponding regulations form the European space of operation including the norms of bio-ethics and bio-security and the informed agreement of the patient. The results of the project will be disseminated and exploited both at level of health services as well as at level of co-financing partners who will develop technology and innovating dental works from improved alloys at reduced costs, fact that emphasizes the co-relation between the thematic of the project and the strategy of development of the SMEs partners. Obtaining a patent and publishing 5 research works in international publications of high impact is a prognosis of result indicators that complete the process indicators. The benefits of implementing the project and its exploitation after ending will grow the quality of life through precocious detection of diseases and their treatment and also through the proposal of a new less dangerous and less toxic alloy. The co-financing SMEs will develop as well the commercialisation capacity subsequent to the production of materials with high market potential.

Developing new graphene-polymer composites biomaterials for scaffold fabrication with applicability in bone repair by coupling multiscale molecular modelling and experiments Call name: Joint Applied Research Projects - PCCA-2011 call, Type 1
PN-II-PT-PCCA-2011-3.1-1538 2012 - 2016

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); UNIVERSITATEA "DUNAREA DE JOS" (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA DE VEST "VASILE GOLDIŞ" ARAD (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.tsocm.pub.ro/cercetare/POLYGRAPH/

Abstract:

The main aims of this project are interdisciplinary efforts using complex computational tools of materials modelling and advanced experimental techniques for the knowledge-based design of novel, improved biopolymer-graphene scaffolds for bone repair. The key functionality of these materials is both physical and chemical cues to direct cells organization, growth, and differentiation in the process of forming functional tissue. The aim is pursued by developing and extensive application of multiscale computer-aided molecular design complemented with computer-assisted evaluation of the end-use performance of the materials in question. The resulting outputs will constitute the doorstep toward the fabrication of the biopolymer-graphene biomaterials of potential commercial values. This goal is achieved by the development of a complex protocol based on advanced experimental techniques. A method for graphene synthesis and modification by plasma treatment in order to introduce different chemical groups on the surface and graft peptides or proteins capable to facilitated cells adhesion, growth, and tissue remodelling will be establish. The end-use performance of these materials depends on several crucial factors such as graphene surface properties, polymer nature, and the methods used for biomaterial synthesis. On the frame of the experimental activity the research will be focus on modulating synthesis parameters in order to obtain a material suitable for bone repair scaffold. Extensive characterisation of biomaterials surface and bulk by physical-chemical techniques will be employed. The ultimate aim of present project should be the in vitro assessment of biocompatibility in terms of cellular morphology, adhesion, viability and proliferation, and the evaluation of differentiation potential of the new elaborated scaffolds. These studies will be complemented with in vivo assay regarding bone reconstruction with biopolymer-graphene scaffolds.

In vitro effects of beta-titanium alloys on biological behavior of macrophages and their cross-talk with osteoblasts Call name: Exploratory Research Projects - PCE-2011 call
PN-II-ID-PCE-2011-3-0737 2011 - 2016

Role in this project: Key expert

Coordinating institution: Universitatea din Bucuresti

Project partners: Universitatea din Bucuresti (RO)

Affiliation: Universitatea din Bucuresti (RO)

Project website: http://www.unibuc.ro/prof/cimpean_a/Proiect-PCE-188-/

Abstract:

The project aims to elucidate the modulation of macrophage function and of the cross-talk between macrophages and osteoblasts by new beta-titanium alloys which are based on titanium and nontoxic alloying elements such as Ta, Nb/Zr. For comparative purposes, a commercial Ti-6Al-4V alloy, will be also investigated. In the first stage, we will investigate if the macrophages cultured in contact with the tested substrates display differences regarding cell viability, proliferation, apoptosis, cytoskeleton and adhesive structures (focal contact, podosomes) which may be important for the interpretation of macrophage function in chronic inflammatory responses. Next, we will evaluate macrophage cytokine, chemokine and matrixin expression at transcriptional and translational levels. The proposed project is an innovative approach which will bring new knowledge on signaling pathways (such as NFκB and MAP-Kinase) that could be activated by various Ti alloy compositions and on material effects upon the cross-talk between macrophages and osteoblasts using a Transwell co-culture model. This cross-talk will be studied in terms of modulation of: i) soluble factors involved in bone turnover (RANKL, OPG); ii) osteogenesis process (alkaline phosphatase activity, mineralization process); iii) macrophage and osteoblast cytokine production. These studies can conduct to the development of cellular models for in vitro assessment of the inflammatory response to osteoprothetic materials.

NEW CONCEPTS AND STRATEGIES FOR THE DEVELOPMENT OF KNOWLEDGE OF NEW BIOCOMPATIBLE STRUCTURES IN BIOENGINEERING Call name: Complex Exploratory Research Projects - PCCE-2008 call
PN-II-ID-PCCE-2008-0248 2010 - 2013

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DIN BUCURESTI-DEPARTAMENTUL DE BIOCHMIE SI BIOLOGIE MOLECULARA

Project partners: UNIVERSITATEA DIN BUCURESTI-DEPARTAMENTUL DE BIOCHMIE SI BIOLOGIE MOLECULARA (RO); INSTITUTUL DE BIOLOGIE SI PATOLOGIE CELULARA NICOLAE SIMIONESCU-LABORATORUL DE CELULE STEM SI TERAPIE CELULARA (RO); UNIVERSITATEA POLITEHNICA BUCURESTI-FACULTATEA DE CHIMIE APLICATA SI STIINTA MATERIALELOR (RO); UNIVERSITATEA POLITEHNICA BUCURESTI-CENTRUL NATIONAL DE CONSULTANTA PENTRU PROTECTIA MEDIULUI (RO); INSTITUTUL DE CHIMIE FIZICA ILIE MURGULESCU (RO); UNIVERSITATEA BABES-BOLYAI CLUJ-NAPOCA, CENTRUL DE BIOMATERIALE, INSTITUTUL DE CERCETARI EXPERIMENTALE SI INTERDISCIPLINARE (RO); INSTITUTUL DE CERCETARE-DEZVOLTARE PENTRU CHIMIE SI PETROCHIMIE BUCURESTI (RO)

Affiliation: UNIVERSITATEA DIN BUCURESTI-DEPARTAMENTUL DE BIOCHMIE SI BIOLOGIE MOLECULARA (RO)

Project website: http://www.pcce248.weebly.com

Abstract:

IN TISSUE ENGINEERING (TE) THE COMBINED KNOWLEDGE FROM BIOLOGY AND ENGINEERING

IS DIRECTED TOWARDS THE POSSIBILITY TO RESTORE LOST OR DAMAGED TISSUE. THE

GENERAL AIM OF THIS PROJECT IS TO CONSOLIDATE A CROSS-DISCIPLINARY TEAM OF

COLLABORATING INVESTIGATORS TO CARRY OUT SOME CELL-SUPPORT CONSTRUCTS (CSC)

WITH POSSIBLE APPLICATIONS IN REGENERATION/REPAIR OF SOFT AND HARD TISSUES AND IT

DOES NOT ASSUME PRE-CLINICAL AND CLINICAL TRIALS. THIS APPLICATION CONTAINS THREE

SPECIFIC OBJECTIVES: 1- OBTAINMENT OF NEW SUPPORT 3-D STRUCTURES DESIGNED TO

CULTIVATE OSTEOBLASTS AND HUMAN MESENCHYMAL STEM CELLS (HMSC) TO OBTAIN CSCS

WITH CHARACTERIZED ARCHITECTURE AND MECHANICAL PROPERTIES, USEFUL IN BONE TISSUE

ENGINEERING; 2 - DEVELOPMENT OF REGENERATION STRATEGIES OF ADIPOSE TISSUE BY

IMPLANTATION OF PREADIPOCYTES IN 3-D HYDROGEL SCAFFOLDS, THAT MIMIC

EXTRACELLULAR MATRIX, DESTINED TO THE RECONSTRUCTION OF SOFT TISSUE DEFECTS

(SEVERE TRAUMAS, DEEP BURNS OR TUMOR RESECTIONS) AND 3 - STUDY OF THE EFFECTS OF 3-

D CULTURE AND GROWTH FACTORS ON THE CHONDROGENIC DIFFERENTIATION OF HMSC CELLS

TO OBTAIN SOME INVESTIGATION MODELS OF THEIR POTENTIAL IN CARTILAGE TISSUE

REGENERATION. THE PROJECT PRESENTS VIABILITY, INNOVATION, COMPLEXITY AND

INTERDISCIPLINARY EXCHANGE BECAUSE: 1 - IT IS A CONSORTIUM WHICH CONSISTS FROM

PARTNERS WITH COMPLEMENTALLY COMPETENCES WHO ENGAGE TO ACT AS A UNITY IN THE

FOLLOWING FIELDS: CELLULAR AND MOLECULAR BIOLOGY, CHEMISTRY AND PHYSICS OF

MATERIALS, ENGINEERING SCIENCES – IN ORDER TO GET ALL OBJECTIVES; 2 - THE PARTNERS

ARE STAFFS WITH A GOOD, STRONG REPUTATION ON THEIR FIELD, AND THEY HAVE THE

NECESSARY MANAGERIAL EXPERIENCE AS WELL AS THE HUMAN RESOURCES AND PERFORMING

EQUIPMENTS AND 3 - THE PARTNERSHIP WAS PARTIALLY CONSOLIDATED DURING PREVIOUS

COLLABORATIONS AND WHICH SUSTAIN IT. ACCOMPLISHMENT OF THE OBJECTIVES OF THIS

PROJECT CONSTITUTES A SCIENTIFIC CHALLENGE FOR ANY SCIENTIFIC TEAM AROUND THE

WORLD.

EEA Mobility Grant - Sorina DINESCU Call name: EEA - Proiecte de Mobilităţi
EEA-MG-RO-NO-2018-0246 2018 -

Role in this project: Project coordinator

Coordinating institution: University of Bucharest

Project partners: University of Bucharest (RO); Institute of Cancer Research- Oslo University Hospital (NO); Oslo University Hospital (NO)

Affiliation: University of Bucharest (RO)

Project website:

Abstract:

FILE DESCRIPTION	DOCUMENT
List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
R&D activities in enterprises
Peer-review activity for international programs/projects