BrainMap

Mrs. Mariana Carmen Chifiriuc

Professor

Professor - UNIVERSITATEA BUCURESTI

Researcher | Teaching staff | Scientific reviewer

Curriculum Vitae (26/06/2024)

Expertise & keywords

Transcriptomic landscape of host-pathogen-antibiotic interactions in Drosophila melanogaster model infected with Gram-negative ESKAPE bacteria Call name: P 4 - Proiecte de cercetare exploratorie - PCE-2021
PN-III-P4-PCE-2021-1797 2022 - 2024

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation:

Project website: https://unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte-de-cercetare/proiecte-cu-finantare-nationala/transcend/?lang=en

Abstract:

While the antimicrobial resistance crisis continues to grow and spread and the discovery of new antibiotics is slow, it is acutely important to improve current antibiotics use. The in vitro assays of antibiotic susceptibility have a low predictability on the antibiotics efficiency in treating multi-resistant bacterial infections, overlooking the complexity of interactions occurring in vivo. To surpass these limitations, we will use for the first time the powerful Drosophila melanogaster model to evaluate the effects of ESKAPE pathogens and antibiotics on D. melanogaster and its microbiota. In this purpose, D. melanogaster males will be infected with Gram-negative ESKAPE bacteria and treated with colistin and carbapenems. Then, state-of-the art methodology (molecular biology, omics and genetic methods, as well as biostatistics and bioinformatic approaches) will be applied. The in vitro and in vivo antibiotic resistance genes expression profiles and the eukaryotic genes expression profiles expected to be triggered by infection and antibiotic treatment will be assessed. This approach will allow us to unveil some of the key determinants of bacterial resistance expression and of D. melanogaster genome and microbiome functional modulation by infection and treatments and antibiotic efficacy in vivo. Hence, advances in our ability to predict efficacy of last resort antibiotics for curbing difficult infections are reasonably expected.

Long-lAsting Innovative NanoCoatings for hEriTage preservation Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-2526 2022 - 2024

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA NAŢIONALĂ DE ŞTIINŢĂ ŞI TEHNOLOGIE POLITEHNICA BUCUREŞTI

Project partners: UNIVERSITATEA NAŢIONALĂ DE ŞTIINŢĂ ŞI TEHNOLOGIE POLITEHNICA BUCUREŞTI (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation:

Project website: https://www.micronanotech.ro/acoperiri-nanostructurate-inovatoare-de-lunga-durata-pentru-conservarea-patrimoniului/

Abstract:

The scope of the current project proposal is to develop innovative nanostructures for the treatment of various surfaces such as stone, cement-based composites, glass, wood, or other materials, in order to ensure the detergency of the system, anti-adherent, antibacterial and antifungal activity, as well as sealant and healing capacity of the cracks. For this purpose, new silica-based coatings (monosiloxane with different functional groups and disiloxane) will be considered and further used as linkers for the decoration with Ag, Au, Cu, ZnO, TiO2 nanoparticles. The siloxane-based solutions called coupling agents can react with nanoparticles, by chemically binding Au, Cu, Ag nanoparticles via thiol groups or ZnO or TiO2 nanoparticles (NPs) via disiloxane agents, conferring complex surfaces with the above-mentioned specific properties and, important to mention, having long-lasting efficiency. The multiple currently available solutions for treating these surfaces are aiming both to prevent the formation of biofilms and to change the appearance of the treated surfaces. These systems are based on various chemical methods using specific detergents and biocides, mechanical cleaning using various cleaning technologies such as sandblasting, laser cleaning or biological cleaning using enzyme-based detergent systems or a mixture of enzymes and bacteria that have the role of cleaning the affected surfaces. The major disadvantage of these solutions is represented by the fact that the usual treatments are offering only short/ medium term protection to these surfaces, thus requiring the frequent repetition of these treatments. Moreover, the biocidal compounds are reaching the natural environment, raising pollution issues, but also inducing the degradation of the treated surfaces, as recently happened with some of the Brâncuşi masterpieces, such as “Poarta sărutului” and “Masa tăcerii”.

Development of a rapid method based on flow cytometry for testing disinfectants efficacy against problematic nosocomial agents Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-2115 2022 - 2024

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation:

Project website: https://unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte-de-cercetare/proiecte-cu-finantare-nationala/fast-det-pd-620/

Abstract:

Healthcare-associated infections (HAI) are among the priority public health problems al global level, by the high morbidity and mortality rates, often occurred as a consequence of resistance development, generating important economic and social burden. Thus, resistance to disinfectants and co-/cross-selection for antibiotic resistance (AR) are serious problems in managing infection control. The project addresses the limitations of standard, time-consuming, culture-based methodologies, by proposing the development and validation of a flow cytometry method (FCM) for disinfectants efficacy testing, under practical, in-use conditions. The FCM method will provide a rapid and accurate tool, allowing the quantification and differentiation of live, dead or viable, but not cultivable bacteria. By providing faster results on disinfectant efficacy against antibiotic resistant nosocomial pathogens, the FCM method will assure a more rapid and efficient selection of an efficient disinfectant, and thus contribute to the prevention of cross-contamination and spread of HAI, as well as to the reduction of the selective pressure for AR. For achieving the above aim, we propose the following specific objectives: O1. Design and optimization of the FCM experimental protocol for disinfectant efficacy testing. O2. Validation of the FCM method against the current CEN standard methods. We will investigate if the damaged cells resuscitate and grow in some environmental conditions or at least retain their living properties and remain non-culturable. Highlighting the presence of such microbial populations on a surface could provide a very effective tool for investigating, controlling and limiting the environmental charge of bacteria with potential to spread and cause nosocomial infections, allowing more rapid and efficient response actions (saving time, money and/or reducing number of deaths).

Antibacterial biocides in the water cycle – an integrated approach to assess and manage risks for antibiotic resistance development Call name: P 3 - SP 3.2 - Proiecte ERA.NET - COFUND
COFUND-AQUATIC POLLUTANTS-BIOCIDE 2021 - 2024

Role in this project: Partner team leader

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); University of Gothenburg (SE); Institute of Marine Research Contaminants and Biohazards (NO); Federal Institute for Materials Research an Testing (BAM) MAterials and Environment (DE); Umea University (SE); Technical University of Denmark (DK); University of South Bohemia in Ceske Budejovice (CZ)

Affiliation:

Project website: https://unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte-de-cercetare/proiecte-cu-finantare-nationala/biocide/

Abstract:

The overall aim of BIOCIDE is to determine how antibacterial biocides contribute to the development and spread of antibiotic resistant bacteria in different aquatic/marine ecosystems, and to inform and enable measures that ultimately protects human health and safe water resources for both humans and wildlife. The knowledge created and its impact will reach well beyond the European setting. We will provide means to guide action both at the source (approval), and in other parts of the water cycle. Predicted No Effect Concentrations and new methodology will facilitate possible future inclusion in regulatory systems, in Europe and elsewhere. The maritime sector will receive guidance to improve sustainable transports by a better understanding of potential human health risks associated with the use of antifouling agents. The research has high relevance for all three JPIs and for several themes within the call, particularly those related to risk assessment and management.

Innovative multifunctional, bioactive topical formulation for the management of malignant wounds Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-2193 2022 - 2024

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); SANIMED INTERNATIONAL IMPEX S.R.L. (RO); INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI" (RO)

Affiliation:

Project website: https://bios.unibuc.ro/Proiecte/proiect-pn-iii-p2-2-1-ped-2021-2193.html

Abstract:

Malignant fungating wounds (MFW) are incurable, severe, and debilitating conditions normally occurring in the last (six) months of oncologic patient’ life, affecting the overall quality of life and requiring appropriate palliative care to alleviate pain and suffering. MFW’s microbiome may influence the development and severity of wound symptoms, causing foul odors, peri-wound skin damage, inflammation, and ultimately, delays in the healing proliferative phase. ARGOS project is implemented by a consortium of two research institutions and one company with previous experience in developing collagen-based pharmaco-cosmeceutic products and is aiming to develop an innovative multi-purpose, bioactive topical formulation to assist the physical management of malignant fungating wounds, by addressing the microbiome-related symptoms such as pain, odor, and exudate. The proposed solution is based on collagen and inorganic - gold, silver and magnetic – nanoparticles functionalized with plant-derived bioactive compounds (such as polyphenols contained in Chelidonium majus L. and Tamarix gallica extracts, with previously demonstrated antioxidant, antimicrobial, antiproliferative, antiviral and immunomodulatory activity). Therefore, the main novelty of the proposed solution is that it provides not only a wide antimicrobial effect, but also anti-cancer activity and pro-healing properties on the site of malignant wounds. The physico-chemical, mechanical and biological properties of the collagen-based optimized product will be validated by standard protocols and the technological scaling documentation and argumentation of the investments required for in vivo tests will be elaborated (TRL4).

Installation for the automated control of the reproducibility of collagen gel batches for biomedical and cosmeceutical applications Call name: P 2 - SP 2.1 - Proiect de transfer la operatorul economic
PN-III-P2-2.1-PTE-2021-0429 2022 - 2024

Role in this project: Key expert

Coordinating institution: SANIMED INTERNATIONAL IMPEX S.R.L.

Project partners: SANIMED INTERNATIONAL IMPEX S.R.L. (RO); INOE 2000 - FILIALA INSTITUTUL DE CERCETARI PENTRU HIDRAULICA SI PNEUMATICA BUCURESTI RA (RO)

Affiliation:

Project website: https://www.sanimed.ro/proiect-remedial_37.html

Abstract:

The project aims to significantly improve the performance of the pilot plant for the production of highly purified fluid, semi-solid and solid collagen forms, which operates in the company S.C. Sanimed International Impex S.R.L., using elements of technological process quality engineering. In concrete terms, the improvement will aim at ensuring the reproducibility of the batches and the traceability of the processing of the material being processed, by developing an automated control system of the technological and operating parameters of the pilot plant. Reproducibility will be improved by replacing the manual control with the automated one, and the traceability of the operation will be implemented by sampling in real time and storing in integrum and ad tempus indefinitum the numerical data provided by the sensors which will be added to the installation, as well as their subsequent processing.

The overall objective of the REMEDIAL project is to (i) bring the pilot plant to the functional parameters required by the GMP (Good Manufacturing Practices) provisions in the field of production of biomaterials and medical devices of IIb and III classes, (ii) to prepare the plant for production certification, according to European regulations for the CE mark and (iii) increase the productivity of the installation, by eliminating unproductive times, repeated processing and fraction of tissue debris and residues resulting from incomplete processing of the raw material (horse or cattle tendons).

The project will ensure the transfer of know-how and constructive-applicative solutions from the National Institute of Hydraulics and Pneumatics Bucharest, to the Sanimed company. The object of the transfer is the concrete realization of the system for the control and automation of the operation of the pilot installation for obtaining the highly purified collagen forms, in operation in the productive spaces of the company.

SYNERGICSTIC ACTIVES SYSTEMS FOR REGENERATIVE COSMETICS Call name: P 2 - SP 2.1 - Proiect de transfer la operatorul economic
PN-III-P2-2.1-PTE-2021-0303 2022 - 2024

Role in this project: Key expert

Coordinating institution: SANIMED INTERNATIONAL IMPEX S.R.L.

Project partners: SANIMED INTERNATIONAL IMPEX S.R.L. (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU TEHNOLOGII IZOTOPICE SI MOLECULARE I N C D T I M (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation:

Project website: https://www.sanimed.ro/proiect-grace_36.html

Abstract:

The GRACE project aims to obtain and characterize a range of integrative products, designed to regenerate the epidermis affected by superficial lesions, solar radiation, radiation therapy, or bedsores, using specifically functionalized hydrogels with complexes of inclusion of active ingredients in host molecules. These supramolecular complexes will represent the innovative component in the formulation of a new range of products with applications in the field of regenerative cosmetics. In concrete terms, the GRACE project aims to obtain a range of 3 products: single-dose hydrogel (for superficial lesions), cream gel (for skin affected by radiation) and cleansing gel (for gentle debris removal and cleaning of superficial lesions). In this sense, the project will stimulate the investment in research - technological development from the beneficiary company, as well as the consolidation of the company's potential actor on the domestic and international market, highly competitive, of dermato-cosmetic products. In the alternative, the project will develop the company's traditional partnerships with scientific research institutions, in order to connect Romanian applied research to the requirements of the national and European socio-economic environment.

Innovative strategies to improve the performances of medical grade metallic surfaces Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-3178 2022 - 2024

Role in this project: Partner team leader

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation:

Project website: http://innovamed.lspi.inflpr.ro/

Abstract:

The use of metallic implants for bone implantation and replacement is an actual trend. However, their success can be undermined by poor osseointegration and infection occurrence, the two main problems associated to implant failure and revision surgeries.

The main objective of InnovaMed is to obtain improved implant-like surfaces synthesized by laser technologies, to contribute to early osseointegration and the prevention/eradication/control of severe implant associated infections.

We will improve the conventional medical grade stainless steel surfaces by transforming them into corrosion free surfaces. This will be achieved by surface coating with a functional ZnO layer by pulsed laser deposition (PLD). The obtained ZnO coated surface will be covered with a multifunctional layer consisting of bioglass combined with statins and antimicrobial substances, which will allow for a fast osseointegration, and slow drug delivery and release. Due to the delicate nature of the organic mixtures, a gentler laser deposition technique will be used: matrix assisted pulsed laser evaporation (MAPLE).

Development of novel scar healing hydrogels with active molecules encapsulated into lipid carriers Call name: P 1 - SP 1.1 - Proiecte de cercetare Postdoctorală - PD-2021
PN-III-P1-1.1-PD-2021-0748 2022 - 2024

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation:

Project website: http://www.hydroscar.com

Abstract:

Nowadays, research and development of dermato-cosmetic formulations is focused on creating controlled release transport systems. Several technological advances have been made in the development of new formulations that are able to control the kinetics of target release and the duration of therapeutic activity of actives. Hydrogel-based drug delivery systems are a promising novel approach nowadays for delivery of actives for an extended period of time. The main goal of HydroSCAR project is to obtain and characterize complexes formed by encapsulating bioactive ingredients (such as cannabidiol (CBD), hyaluronic acid (HA) into lipid or polymeric carriers (liposomes, micelles, biopolymers, etc.) and including them in a hydrogel formulation in order to obtain a novel, innovative scar-healing dermato-cosmetic formula. Using nano/microencapsulation processes we attempt to embed potent molecules such as CBD and HA into various carriers and develop actives with improved functionality for potential use as a scar-healing hydrogel. The product envisioned as an end result of this project aims to tackle the last two stages of the wound repair process (proliferation and remodeling); especially in the final stage, when the patients are no longer hospitalized and should be provided with appropriate self-care topical treatments to boost and assist the efficiency of internal treatment, thus regaining fast scar closure, skin elasticity, and improvement in overall appearance and texture.

Mobile genetic elements mapping – first bioinformatic insight into the resistome of ESKAPE clinical and environmental isolates from Romania Call name: P 1 - SP 1.1 - Proiecte de cercetare Postdoctorală - PD-2021
PN-III-P1-1.1-PD-2021-0540 2022 - 2024

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE BOLI INFECTIOASE ''PROF.DR.MATEI BALS''

Project partners: INSTITUTUL NATIONAL DE BOLI INFECTIOASE ''PROF.DR.MATEI BALS'' (RO)

Affiliation:

Project website: http://mariussurleac.ro/mobile-genetic-elements-mapping-first-bioinformatic-insight-into-the-resistome-of-eskape-clinical-and-environmental-isolates-from-romania/

Abstract:

Among European countries, Romania is experiencing one of the highest rates of antibiotic resistance (AR) as revealed by most recent data reported by international surveillance programs such as ECDC, justifying the urgent need for monitoring the AR dynamics in the clinical and environment isolates. This project aims to contribute to the study of mobile genetic elements (MGEs) which are important contributors to the worldwide dynamics of resistance spreading.

This project attempts to characterize in detail the ESKAPE resistome by bioinformatic analyses of various MGEs (e.g., plasmids, insertion sequences, transposons, genomic islands) representing huge pools of resistance genes and sets of such genes. These aspects will be correlated with bioinformatic studies focused on the phylogenetic relationships between such factors, between the isolates from the same species and among species, which in turn will cover some vital aspects of clinical interest, namely, functional traits with impact on the metabolic pathways, biological processes. Another important objective of this proposal is to validate the bioinformatic analysis by the use of experimental methods such as the MinION (Oxford Nanopore) technology for 3rd generation sequencing; this will improve the results by increasing the coverage and the degree of confidence: by using such a method we’ll be able to capture the full length of genes with correct orientation as well as of big MGEs such as genomic islands and plasmids.

Innovative coatings for increasing implants biocompatibility and preventing associated infections Call name: P 1 - SP 1.1 - Proiecte de cercetare Postdoctorală - PD-2021
PN-III-P1-1.1-PD-2021-0598 2022 - 2024

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO)

Affiliation:

Project website: http://biocoat.lspi.inflpr.ro/

Abstract:

The treatment of implant-associated infections can be challenging because of persistence, antimicrobial resistance and / or tolerance (through excessive or misuse of antibiotics). But antibiotic treatments are often ineffective on microbial biofilms, causing infection persistence. An alternative to antibiotic therapy is the use of antimicrobial peptides (AMPs). Compared to antibiotics, microorganisms do not develop resistance to AMPs. The responses of biological systems to implants can be improved by changing the reactivity of material surfaces by stimulating bioactivity and interactions with adjacent tissue. When in the physiological environment of the human body, bioglasses (BG) form a bone-to-material anchorage through the establishment of a carbonated apatite layer on their surfaces, allowing for an improved biocompatibility and preventing immune responses. At the same time, BG can be combined with substances that can boost their bioactivity. This project aims to obtain thin films of BG with AMPs and vitamin D3, providing a solution to two different problems: early osseointegration and resistance to microbial colonization and further development of the biofilm. The thin films will be made using the matrix assisted pulsed laser evaporation (MAPLE) technique, which generates uniform and adherent coatings on various substrates, with controlled thicknesses.

Type 2 Diabetes: a multi-OMIc view on host NAPH oxidases (NOx) and gut microbiota Call name: P 1 - SP 1.1 - Proiecte de cercetare Postdoctorală
PN-III-P1-1.1-PD-2019-0499 2021 - 2022

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte-de-cercetare/proiecte-cu-finantare-nationala/domino/

Abstract:

Development of type 2 diabetes(T2D) is a very complex, gradual and heterogeneous process. Importantly, diabetic individuals exhibit differences in terms of gut microbiome signatures, metabolic profiles and host markers compared to healthy individuals. Even though the field of microbiota analysis has emerged in the last few years and many reports associate microbial changes to various ailments, integrative studies that target the host-microbe interactions in diabetes are still in their infancy. To gain a better understanding of the biological processes linked to T2D, we will perform a detailed longitudinal multi-omic analysis of diabetic patients compared to healthy individuals targeting the microbiome, metabolome and resistome profiles. Altogether, these analyses will provide a rich source of data to characterize the host-microbe interactions associated with T2D.

Novel strategies to improve the performances of medical textiles Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-4926 2020 - 2022

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://lspi.inflpr.ro/2020/PED555/IMEDTEX

Abstract:

We propose a new generation of chronic wound dressings with enhanced healing rates and protection of the lesion from outside pathogens. Medical grade cloth will be surface engineered on the side in contact with air, to become superhydrophobic and antimicrobial via thin film coating with black ZnO (nonstoichiometric, vacancy, defect containing ZnO), synthesized by Pulsed Laser Deposition.We showed in a previous research that black ZnO is both superhydrophobic and antibacterial/antifungal. The inner side of dressings, in contact with the wound, will be covered by Matrix Assisted Pulsed Laser Evaporation with a hydrophilic mixture consisting of antibiotics, epithelial growth factors and analgesic. Linezolid/dicloxacillin/neomycin will be used as antibiotics with efficiency against both Gram negative and Gram positive bacteria, hyaluronic acid as growth factor in tissue reconstruction and a neuropathic agent with analgesic and anti-inflammatory effects. The novelty of the Project comes from the use of laser deposition techniques which can be tuned to synthesize both organic and inorganic adherent coatings with precise control of thickness and minimal consumption of precursors.

The correlation between coating’s properties of interest and their morphology or composition will be made after thorough tests by atomic force and scanning electron microscopy, profilometry, wettability assessment, Xray diffraction and Fourier transform infrared spectroscopy.

ZnO films antimicrobial properties will be studied against Gram positive and Gram negative bacteria (bactericide effect, antibiofilm assessment). The biocompatibility and the efficiency of healing factors in form of thin films will be studied in vitro on epithelial lines of cells.

Nano-modified surfaces for G-tubes resistant to microbial colonization Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-3829 2020 - 2022

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://nanopeg.inflpr.ro/

Abstract:

Despite their great benefits for debilitated patients, gastrostomy tubes (G-tubes) are prone to become easily colonized by resident and opportunistic microorganisms, which have the ability to attach on their surfaces and form highly specialized communities called biofilms, which are extremely resistant to host defense mechanisms and antibiotic treatment, leading to treatment failure and device replacement. The scope of this project is to develop an improved G-tube with nano-modified, non-citotoxic and uniform surface, containing core/shell magnetite@silica nanoparticles functionalized with the plant derived compound eugenol (Fe3O4@SiO2@E), to limit microbial colonization and biofilm formation on such devices. The product will consist in a nano-modified surface (obtained by matrix assisted pulsed laser evaporation, MAPLE), biocompatible, resistant to microbial colonization and biofilm formation, to obtain improved G-tubes. We will start with the synthesis and characterization of the nanosystem, followed by the design of nano-modified G-tube surfaces, their quality assessment through physico-chemical, microbiological tests and cytotoxicity assays. By the proposed approach, our project falls within the TRL2-TRL3 level; since after observing the basic principles, we developed an application (antimicrobial and antibiofilm nanosystem) (TRL2), which can now be translated into an improved device (G-tubes with nano-modified surfaces by using nanosystems functionalized with E) able to avoid microbial colonization and biofilm development (TRL3). By its innovative and multidisciplinary approach, this project brings important advances on the field of improved indwelling devices, which, in the current context of the antibiotic crisis and biofilm-related infections represent a top priority at national and international level.

COLD PLASMA FOR FLUORIDE RETENTION IMPROVEMENT AND BIOFILM MODULATION IN DENTAL APPLICATION Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-4569 2020 - 2022

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE "CAROL DAVILA" (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://alina.amgtranscend.org/?page_id=87

Abstract:

Despite the technological progress of the last decade, dental caries is still the most frequent oral health threat in children and adults alike. Such condition has multiple triggers and is caused mainly by enamel degradation under acidic attack of microbial cells, which compose the biofilm of the dental plaque. The biofilm of the dental plaque is a multispecific microbial consortium, which periodically develops on mammalian teeth, and it can be partially removed through mechanical forces by individual brushing or in specialized oral care medical facilities. Inhibition of microbial attachment and biofilm formation, as well as methods to strengthen dental enamel to microbial attack represent the key factors in caries prevention. The purpose of this project is to elaborate a cold plasma based method in order to modulate microbial attachment and biofilm formation and to improve the retention of fluoride in an enamel-like model. In our approach, the obtained cold plasma will be tested for its capacity to determine fluoridation and improve fluoride retention in a hydroxyapatite based enamel-like material. Moreover, the impact of such cold plasma on the development of microbial biofilms under various relevant conditions will be established by in vitro studies. In this project, we will start with a technology-formulated concept based on a cold plasma treatment (TRL 1-2), and we will end by validating the technology in laboratory conditions, enlightening the plasma ability to improve fluoride retention and modulate microbial biofilm formation in an enamel-like hydroxyapatite based model (TRL 4). The obtained cold plasma with dual effect exhibited through biofilm modulation and enamel strengthen through fluoridation is intended for dental application such as the prevention and therapy of dental caries and enamel deterioration.

Selection and dissemination of antibiotic resistance genes from wastewater treatment plants into the aquatic environment and clinical reservoirs Call name: P 4 - Proiecte Complexe de Cercetare de Frontieră
PN-III-P4-ID-PCCF-2016-0114 2018 - 2022

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE -DEZVOLTARE PENTRU ECOLOGIE INDUSTRIALA - ECOIND (RO); INSTITUTUL NATIONAL DE BOLI INFECTIOASE ''PROF.DR.MATEI BALS'' (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE MEDICO-MILITARA „CANTACUZINO” (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://bios.unibuc.ro/Proiecte/pn-iii-p4-id-pccf-2016-0114.html

Abstract:

The RADAR project aims to assess the prevalence and dissemination of antibiotic resistance (AR) from urban, clinical and industrial wastewater into the aquatic environment via wastewater treatment plants (WWTPs). Moreover, RADAR will give information on the environmental and clinical resistome, identifying the possible mechanisms of AR emergence and spread.

Scientific objectives: i) investigation of WTTPs resistome to establish the prevalence of AR bacteria (ARB)/genes (ARGs) in the environmental samples from upstream-WWTP-downstream transects; ii) evaluation of selected antibiotics (beta-lactams, fluoroquinolones and macrolides) occurrence in both WWTP’ influent and effluent; iii) comparative analysis of geographically and time related ARB/ARGs from wastewaters and clinical sources; iv) assessing and prediction how the presence/absence of ARGs and their relative abundance depending on a class of geographical, hydrological, physico-chemical and microbiological factors.

Methodology: The urban, clinical, farming and industrial wastewater will be monitored by physico-chemical, microbiological methods and metagenomics throughout various steps, i.e.: 1000 m upstream river, influent, different treatment steps inside WWTP, effluent and 200 m downstream river. Wild fish from the receiving river after discharge of WWTP effluent will be also analyzed. The following analyses will be performed: i) LC/MS and HPLC techniques to monitor the levels of selected antibiotics; ii) isolation and identification of ARB belonging to ESKAPE species; iii) evaluation of metagenomic resistome by bioinformatic data processing; iv) environmental and clinical ARGs and plasmids sequencing; v) analyzing the potential of ARGs for transferability and environmental/clinical risk by mapping the insertion loci of transposable elements and the ARGs potential to mobilize the resistance genes in a bioreactor model; vi) elaboration of a prediction model for the occurrence of ARB/ARGs

Advanced techniques and increasing performance in the early detection of SARS-CoV-2 virus Call name: P 2 - SP 2.1 - Soluţii - 2020 - 1
PN-III-P2-2.1-SOL-2020-0090 2020 - 2021

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE- DEZVOLTARE PENTRU MICROTEHNOLOGIE - IMT BUCURESTI INCD

Project partners: INSTITUTUL NATIONAL DE CERCETARE- DEZVOLTARE PENTRU MICROTEHNOLOGIE - IMT BUCURESTI INCD (RO); UNIVERSITATEA BUCURESTI (RO); SPITALUL UNIVERSITAR DE URGENTA BUCURESTI (RO)

Affiliation:

Project website: https://www.imt.ro/kit-SARS-CoV-2/

Abstract:

This project proposes to obtain and validate "Point-of-Care" (POC) methods and kits for early diagnosis of SARS-CoV-2 infection. The aim is to develop original solutions for: viral RNA detection kit using LAMP isothermal amplification, anti-SARS CoV 2 antibody detection kit, viral antigen detection kit and a rapid test kit by multiplexing the system (for schools, institutions, points border). The kits will be of the "sample-to-answer" type, a plastic cartridge that supports a microfluidic system on paper. The proposed methods are cheap (3-4 Euro / piece), fast and precise (the LAMP method of isothermal amplification of nucleic acids is qualitative at the level of the "golden method" - rtPCR). Nasopharyngeal exudate and saliva (recently approved by the FDA) will be used as biological samples. Validation will be done on clinical trials by reference to the classical rtPCR method. By implementing the viral RNA detection kit for the saliva sample, Romania can become the first country in the world to introduce pandemic testing at home. The proposed solution may have a decisive effect in reducing SARS CoV2 infection, but also with a media effect in the international press and the scientific community.

Selection and dissemination of antibiotic resistance genes from wastewater treatment plants into the aquatic environment and clinical reservoirs Call name:
PN-III-P4-ID-PCCF2016-0114 2018 - 2021

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE MEDICO-MILITARA „CANTACUZINO” (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website:

Abstract:

INNOVATIVE BIONANOMATERIALS FOR TREATMENT AND DIAGNOSIS Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0629 2018 - 2021

Role in this project: Partner team leader

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE "CAROL DAVILA"

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE "CAROL DAVILA" (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE TARGU MURES (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://umfcd.ro/cercetare-si-dezvoltare/proiecte/proiecte-nationale/bionanomateriale-inovative-pentru-tratament-si-diagnostic/

Abstract:

In the field of curing different maladies the current medicine make special efforts for the control of factors that favor their appearance together with pathogen agent surveillance. The development of modern medicine is strongly influenced by technology and in this view together with the development of tissue engineering it was tried the creation of a replacement for human bony tissue. In the preset project the research efforts from the forth component projects are implied in:1- antimicrobian nanomaterials; 2- biomaterials for tissue regeneration and for diagnosis, prevention and treatment of osteoporosis; 3- intelligent nanocomposites for diagnosis and treatment bony cancer; 4- nanostructured probes dedicated to diagnosis. The research concerning the obtaining of new intelligent bioceramics with antiseptic properties dedicated to bony tissue regeneration together with their physical-chemical characterization implies the elaboration of protocols for obtaining, analysis and prophylaxis at laboratory animals tests level. Biomaterials application in the field of tissue regeneration for osteoporosis case will bring experimental data related to structural and morphological characterization of nanomaterials together with information regarding biocompatibility and cytotoxicity. These data that will lead to composites optimization as a function of histological character common to laboratory animals. The study of intelligent nanocomposites will open a direction related to their action in bony cancer case. The development of photoluminescent biocompatible ceramic nanomaterials is dedicated to bony screening and further more will their functionalization with anti-inflammatory materials in order to evaluate their in vivo action and their possible using as biological probes. The statistics for the osteoporosis appearance in different population decades indicate their maximum in the (41-60) age limit, and in this view, the studies presented in this project are of general import

Interinstitutional program of developing advanced eco-nanotechnology solutions for multifunctional treatments of leather and textile materials Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0743 2018 - 2021

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA SI INGINERIE NUCLEARA " HORIA HULUBEI " - IFIN - HH

Project partners: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA SI INGINERIE NUCLEARA " HORIA HULUBEI " - IFIN - HH (RO); INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU TEXTILE SI PIELARIE - INCDTP BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU TEHNOLOGII IZOTOPICE SI MOLECULARE I N C D T I M (RO); UNIVERSITATEA BUCURESTI (RO); INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI" (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://www.nipne.ro/proiecte/pn3/7-proiecte.html

Abstract:

Developing integrated functionalization eco nano technologies using physical techniques (gamma irradiation, plasma activation, electrodeposition) and nanocomposites with antibacterial, antistatic or hydrophobization properties will produce textile and leather materials with multifunctional advanced properties. Replacing and reducing volatile organic compounds that negatively impact the environment will set the premises of transferring advanced technologies to companies manufacturing medical articles, protection equipment, sports equipment or for other applications and creating thus new research services by PHYSforTeL consortium partners to the benefit of the traditional industrial sector of textile and leather industry.

Integrated development project for advanced medical treatment technologies Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0728 2018 - 2021

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE MEDICO-MILITARA „CANTACUZINO” (RO); INSTITUTUL DE BIOCHIMIE (RO); UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA PITESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://teramed.inflpr.ro/

Abstract:

In order to increase community’s quality of life, the aim of the project proposal entitled “Integrated development project for advanced medical treatment technologies” (TERAMED) is to develop novel technologies with respect to the treatment of osseous and cutaneous conditions and oncological disorders. Given our experience in healthcare research and the current requirements of multidisciplinary and interinstitutional collaboration towards the personalized treatment purpose, the TERAMED project aims genuine synthesis and processing of biomaterials, but also functional and therapeutic evaluation relevant for clinical trials. The main objectives of the “Medical devices functionalized by laser technologies and alternatives for enhanced osseous integration and regeneration” subproject are to design and produce inorganic, composite or hybrid coatings for superior osteoconductive and osteoinductive performances of titanium-based implants. Smart wound patches and polymeric gels functionalized with antimicrobial and wound healing biomolecules incorporated within micro- and nanoparticles constitute the purpose of the “Medical devices (patches and gels) based on composite biomaterials obtained by laser, plasma and radiation technologies and alternatives for enhanced healing of cutaneous injuries” subproject. The “Technologies based on magnetic triggered nanostructures for oncological therapy: early diagnosis and targeted treatment” subproject aims the development of multifunctional medical devices for specific and selective diagnosis and treatment of breast cancer and melanoma. The general impact of the TERAMED project ensues from the beneficial conjunction of the clinical potential of the proposed medical devices, the feasible technological transfer and the economic advantages of interinstitutional collaboration.

Advanced Innovative approaches for predictable regenerative medicine Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0782 2018 - 2021

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE IN DOMENIUL PATOLOGIEI SI STIINTELOR BIOMEDICALE "VICTOR BABES" (RO); INSTITUTUL ONCOLOGIC PROF.DR.I.CHIRICUTA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte-de-cercetare/proiecte-cu-finantare-nationala/65pccdi-2018/

Abstract:

Considering that HEALTH is one of the main areas of public priority, the approach of interdisciplinary researches with applicability in Regenerative Medicine can significantly contribute to improving the quality of life of patients with tissue defects. The aim of the project is to create a consortium with complementary research experience in the field of regenerative medicine, which will efficiently use the human resource and modern research infrastructures newly created for the implementation of innovative technologies, with the aim of developing and transferring the results to the economic environment. The project aims to develop new technological products in the form of biocompatible biomaterials designed for bone reconstruction (P1), nerves (P2), soft tissues (P3) and breast reconstruction after tumor resection (P4).

Advanced biodegradable materials based on MgB2 resistant to microbial colonization Call name: P 3 - SP 3.2 - Proiecte ERA.NET - COFUND
COFUND-M-ERA.NET II-BIOMB 2017 - 2021

Role in this project: Partner team leader

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA

Project partners: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://infim.ro/project/biomb/

Abstract:

The innovation of this project consists in the evaluation for the first time of the MgB2 potential for biomedical applications, although it is currently produced for superconductivity devices. Expectations are to generate new MgB2-based composite multifunctional biomaterials with antimicrobial/antifouling properties, and an increased biocompatibility at interfaces between the material and the biological media.

The MgB2 powders, coatings and bulks could be used in biodegradable implants or drug delivery systems, handles and surgical tools, catheters, wound dressings and so on. The mechanical and physico-chemical properties of the proposed materials will be investigated by a comprehensive approach, and bioevaluation will include in vitro and in vivo assays. The MgB2 materials are viewed as solutions for space and time- scale controlled variation of the functional properties required for different bio-applications.

Antimicrobial Resistance Manure Intervention Strategies Call name: P 3 - SP 3.2 - Proiecte ERA.NET - COFUND
COFUND-JPI-EC-AMR-ARMIS 2018 - 2021

Role in this project: Partner team leader

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://bios.unibuc.ro/Proiecte/cofund-jpi-ec-amr-armis.html

Abstract:

Manure is one of the major sources of antimicrobial resistance (AMR) in the environment, since

livestock animals consume the majority of antibiotics produced globally. Antibiotics together

with antibiotic resistant bacteria are excreted to the environment via manure, and may

significantly contribute to the transmission of and exposure to AMR in food, water, and air as

exemplified for methicillin-resistant Staphylococcus aureus (MRSA). Techniques for nutrient

reduction in manure, such as composting and anaerobic digestion, exist and are started to be

applied in a number of countries. These techniques can also reduce antibiotic resistance.

However, to date, no studies simultaneously studied the reduction of all AMR components

(antibiotics, bacteria and genes) by different manure interventions.

In this project, we will measure the effectiveness of different manure treatment techniques on

AMR reduction throughout the manure chain and analyse process parameters of influence.

Both large-scale (centralised) treatment systems and small-scale (farm) systems will be

evaluated in different national contexts with varying AMR prevalence. With culture dependent

and culture independent methods, the abundance of antibiotic resistance and its mobility at

different steps of the manure treatment processes is evaluated. We will focus on ESBLproducing

Enterobacteriaceae, vancomycin-resistant Enterococci (VRE) and MRSA, as well

as metagenomic and qPCR analyses of antibiotic resistance genes, and quantification of

antibiotic residues. Emissions of AMR from manure and manure treatment systems will be

assessed, in order to determine risks of exposure. With input from workshops on risk

perception with relevant stakeholders, these risk assessment outcomes will be communicated

to improve awareness on antibiotic usage, to guide prioritising intervention initiatives, and to

further comprehend exposure risks. Knowledge on the effectiveness of manure interventions

on AMR transmission may contribute to reducing the AMR

impact caused by the livestock industry.

From the classical nutrition to precise nutrition in animal production, scientific basis for the nutrition security of the population Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0473 2018 - 2021

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA - IBNA BALOTESTI

Project partners: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA - IBNA BALOTESTI (RO); UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA BABES BOLYAI (RO); INSTITUTUL DE BIOCHIMIE (RO); UNIVERSITATEA "ŞTEFAN CEL MARE" DIN SUCEAVA (RO); UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.ibna.ro/proiecte-de-cercetare/item/110-pn-iii-p1-1-2-pccdi-2017-0473

Abstract:

The overall objective of the project is to strengthen the institutional capacity of IBNA by developing new research directions based on „omics” technologies – nutrigenomics, toxicogenomics, proteomics, metabolomics – and to relaunch the Laboratory of Biotechnology. This will generate a set of innovative outcomes in animal nutrition. The innovative aspects of the project include: use of agro-food wastes as adsorption and detoxifying agents for feed contaminants; testing the effect of phytoadditives (plants or plant extracts not yet used for such purposes) as antibiotic replacers (willow bark extracts) on the interaction between the intestinal tract physiology and the intestinal microflora; investigation of several less characterized and used protein-oleaginous sources (cowpea, Jerusalem artichoke, etc.); in vitro experimental models to evaluate the decontaminating potential of wastes under conditions of concurrent fungal, viral and microbial contamination; development of new feeding products which ensure a more efficient use of the protein in ruminants.

The project covers three regions of development and the partners are prestigious institutions, with complementary infrastructure and expertise, with converging scientific interests. The consolidation of new directions will enhance, both for IBNA (the relaunched institution), and for its partners, their capacity of applying for/running research projects, allowing studies with a high level of complexity, which generate practical results, relevant for the development of the animal production sector.

The project will impact on the human resources, the budget allowing the employment of 14 researchers (of which 10 by IBNA), whose specialisation is already ensured; it will also support the enhancement of the scientific expertise of the senior researchers by using vouchers. The training periods will enrich the portfolio of methods and techniques that can be applied for the directions in which IBNA aims to develop/relaunch.

New textiles for parietal defects Call name: P 3 - SP 3.2 - Proiecte ERA.NET - COFUND
COFUND-ERANET MANUNET III-PariTex 2019 - 2021

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU TEXTILE SI PIELARIE - INCDTP BUCURESTI

Project partners: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU TEXTILE SI PIELARIE - INCDTP BUCURESTI (RO); SANIMED INTERNATIONAL IMPEX S.R.L. (RO)

Affiliation:

Project website: http://www.paritex.ro

Abstract:

The role of medical devices is essential to the healthcare of citizens. The diversity and innovativeness of this sector contributes significantly to enhancing the safety, quality and efficacy of healthcare in the EU. The challenges related to R&D, emerging technologies and the green economy, as well as issues related to the EU’s trade and regulatory cooperation globally have became more intensive in recent years. SMEs in particular face challenges in this regard. The medical devices sector faces many challenges at national, European and international level, which may have an impact on their innovation capacity and overall competitiveness: Public Health Systems, Finding the balance between patient’s needs and financial sustainability, Competitiveness and innovation. The medical devices with high added value made from resorbable and nonresorbable biopolimers, processed by modern technology (knitting and electrospinning) with linear and spatial structure, functionalized with active agents will be obtained in PariTex project will be a contribution of R&D activity to give an answer for sustainable medical textile device sector. The PariTex project will develop new textiles materials for healing of the parietal abdominal defects, based fibers/ yarns made of nonabsorbable and resorbable polymers (biopolymers) obtained by classical and unconventional (electrospinning) technologies. Knitted and nonwoven fabrics characterized by mass, thickness, porosity,density, dimensions etc. are functionalized by incorporating or coating with substances that have the goal of: reducing adherence to human tissue, reducing the risk of infection, monitoring healing time by using controlled release systems based on natural polymers, reducing abdominal discomfort and associated pain. Will be assessed the level of biological performance, by the test of the mutagenic potential by using the micronucleus test on the hematogenic marrow of mice to determine the sensitizing potential, acute local tolerance, cytotoxicity and subacute toxicity.

The project will contribute to the initiation and consolidation of a value chain for the realization of functional textile structures based on partners' competencies, but also by attracting clusters of textiles and medicine for the application of the results.

Innovative technologies based on polymers for the obtaining of new advanced materials Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0428 2018 - 2021

Role in this project: Key expert

Coordinating institution: Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti

Project partners: Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO); INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE CHIMICO - FARMACEUTICA - I.C.C.F. BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU ELECTROCHIMIE SI MATERIE CONDENSATA - INCEMC TIMISOARA (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); UNIVERSITATEA BUCURESTI (RO); INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI" (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://icechim-rezultate.ro/proiect.php?id=41&lang=ro

Abstract:

The project is aimed at using the expertise that involved in consortium entities acquired in materials science. The consortium consists of three representative national institutes: INCD for Chemistry and Petrochemistry – ICECHIM Bucharest, INCD for Electrochemistry and Condensed Matter - INCEMC Timisoara and INCD of Chemical Pharmaceutical - ICCF Bucharest, and of two prestigious universities: University POLITEHNICA of Bucharest and the University of Bucharest and a remarkable institute of Romanian Academy: Institute of Macromolecular Chemistry – Petru Poni Iasi. Although having great tradition and noteworthy results, the first five institutions face a series of problems, from the lack of financial funds, equipment and the aging employees for ICCF and partly for INCEMC and ICECHIM, to the lack of highly qualified staff required for recent investments in equipment for all 5 institutions. In this respect, the project attempts relaunching the activity in the first 5 institutions of the consortium, by putting together the existing competencies, so as to develop new technologies in order to obtain new materials with high performance properties. Given that, 3 of the research teams are specialized in polymers (ICECHIM, Petru Poni and UPB) the developed technologies will use the polymers as intermediates or as a component in the finished product. To this end it is envisaged getting the titanium nitride for prosthetic coatings via inorganic-organic polymer nanocomposites, obtaining photocatalytic materials and antibacterial coatings by sol- gel reactions, obtaining of short-life or one-time use biomaterials from aliphatic polyesters and micro or nanocellulose and the development of new polyphase materials with medium or long life, based on biopolymers, through 3D printing. The project intends the full use of A1, A2, B and C checks in order to increase the institutional performance of partners.

Multidisciplinary platform for integrative and systematic research of tangible and non-tangible cultural heritage and identities in Romania Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0686 2018 - 2021

Role in this project: Partner team leader

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL DE ARHEOLOGIE "VASILE PIRVAN" (RO); INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA SI INGINERIE NUCLEARA " HORIA HULUBEI " - IFIN - HH (RO); UNIVERSITATEA "ALEXANDRU IOAN CUZA" IASI (RO); UNIVERSITATEA DE VEST TIMISOARA (RO); INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA (RO); INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE IN INFORMATICA - ICI BUCURESTI (RO); UNIVERSITATEA OVIDIUS (RO); UNIVERSITATEA DIN ORADEA (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://patcultro.unibuc.ro

Abstract:

The present project represents an original approach, from an inter-, pluri-, multi- and transdisciplinary perspective of the 6th domains - Patrimony and cultural identity. Through the structure of the consortium, the management of the project, the objectives proposed by the component projects, through the synergies and complementarities obtained, undoubtedly presents a high degree of feasibility, being a sophisticated, integrative and exhaustive approach for Romania. Its results will have an impact both on the national and international scientific communities, but also on the broad public, contributing through the proposed activities to the consolidation, development, and improvement of the RDI system in our country, and indirectly to the training of the future specialists from the Romanian research.

The final goal of the project is to create a complex interdisciplinary/multidisciplinary platform for integrated and systematic research of tangible and non-tangible cultural identities and heritage in Romania.

The consortium that will implement this complex project is coordinated by the University of Bucharest (CO-UB), in partnership with the "Vasile Parvan" Institute of Archeology of the Romanian Academy (P1-IAB), the National Institute for Research and Development for Physics and Nuclear Engineering " Horia Hulubei "(P2-IFIN-HH)," Al. I. Cuza "University of Iasi (P3-UAIC), West University of Timisoara (P4-UVT), National Institute of Research and Development for Material Physics (P5-INCDFM), the "Ovidius" University of Constanta (P6-UOC) and the University of Oradea (P7-UO). The way of geographic distribution at the level of Romania ensures a good regional representation of the legal entities represented within the consortium in this project. The combination of five universities and three national research institutes is the ideal combination to support and implement such a complex project.

Antibiotic Resistance in Wastewater:Transmission Risks for Employees and Residents around Waste Water Treatment Plants Call name: P 3 - SP 3.2 - Proiecte ERA.NET
ERANET-JPI-EC-AMR -AWARE-WWTP 2017 - 2020

Role in this project: Partner team leader

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://bios.unibuc.ro/Proiecte/eranet-jpi-ec-amr-aware-wwtp.html

Abstract:

The rise of antibiotic resistant infections is an imminent global public health threat, and mitigation measures are required to minimize the risks of transmission and human exposure. Municipal wastewater treatment plants (WWTPs) are known hotspots for the dissemination of clinically relevant resistant bacteria of human origin to the environment, and simultaneously represent targets for intervention and mitigation strategies. While aerosolized bacteria are found within WWTP, it is largely unknown whether WWTP workers are at risk of elevated resistance carriage. In order to study the occupational and environmental transmission of antibiotic resistance due to human exposure to WWTP-borne bacteria, we will assess carriage of extended-spectrum beta-lactamase (ESBL) and carbapenemase-producing Enterobacteriaceae and resistance genes in WWTP workers, in residents in the proximity of treatment plants, and in water and air samples – both in countries with low and high antimicrobial resistance (AMR). Based on microbial cultivation as well as on high-throughput sequencing data and quantitative real-time polymerase chain reaction (qPCR), exposure through ingestion and inhalation will be modelled, and airborne exposure will be derived from geospatial analyses. Further, we will analyse treatment efficiencies of different WWTP processes in terms of AMR reduction, and therewith identify science-based critical control points for interventions. The focus of this transnational collaboration combining complementary and synergistic European research strengths, is to tackle the increasingly relevant public health threats from antibiotic resistance in WWTP by identifying transmission routes, means of exposure, and proposing risk reduction measures.

Tracking down carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter baumannii isolates from hospital to the aquatic environment via the wastewater network: a pilot study Call name: P 1 - SP 1.1 - Proiecte de cercetare Postdoctorală
PN-III-P1-1.1-PD-2016-1798 2018 - 2020

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://icubmicrobiology.wixsite.com/icub/raport-%C8%99tiin%C8%9Bific-2020-1

Abstract:

Wastewater treatment plants (WWTPs) are providing the perfect environment for the enrichment, recombination, selection and spread of antibiotic resistant (AR) super-bugs. The AR bacteria containing hospitals wastewater is treated by WWTPs, the effluent being discharged into the rivers. This pilot study aims to evaluate the risk of dissemination of carbapenemase- producing, multi-drug resistant Pseudomonas aeruginosa and Acinetobacter baumannii (CP PA AB) clinical strains from the hospital to the aquatic environment via the WW network. Over a three months period, we will sample weekly seven points (hospital WW treated in the urban WWTP from three hospital units, untreated WWTP affluent, WWTP effluent and the river upstream and downstream of the WWTP). The CP PA AB strains will be isolated from the collected WW samples using carbapenem supplemented culture media and the CP PA AB loads will be determined. In the same temporal sequence, CP PA AB strains will be isolated from patients hospitalized in the three hospital units and appropriately preserved for comparative analyses between the clinical and environmental isolates. The CP PA AB strains will be confirmed by molecular identification (rpoB/recA/16s DNA sequencing) and their resistance phenotypes will be established. The carbapenemases genes and their localization will be identified by PCR and gene sequencing, and their transferability assessed by conjugation. The CP PA AB clinical and aquatic samples will be genotyped by pulsed-field gel electrophoresis and multilocus sequence typing. The comparison of the CP PA AB sequence types will allow to: i) track down the fate of the clinical strains in the WW network; ii) establish the phylogenetic relationships between the clinical and aquatic strains; iii) establish the comparative levels of CP PA AB loads in the river upstream and downstream from the WWTP and furthermore iv) to evaluate the impact of the WWTP on the CP PA AB counts in the effluent and receiving river.

Mapping the insertion sites of some transposons involved in antibiotic resistance Call name: P 1 - SP 1.1 - Proiecte de cercetare Postdoctorală
PN-III-P1-1.1-PD-2016-2137 2018 - 2020

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://icubmicrobiology.wixsite.com/icub/proiect-1

Abstract:

Antibiotic resistance (AR) is major health issue worldwide. The understanding of genetic background of AR is a key strategy in order to slow the spread of high-risk multidrug-resistant clones. While many studies are focused on describing the encoding genes of antibiotic-degrading enzymes, still the non-enzymatic genetic background of resistance is poorly studied. Mobile genetic elements (MGE) plays a major role in dissemination of AR encoding genes (ARG). Bacterial simplest MGEs are represented by insertion sequences (ISs) and transposons, which may carry in their structure ARG. As they may transpose (travel) within or between bacterial genomes, they have a major contribution in spreading of AR. Moreover, even this MGEs don't carry an ARG, they can be involved in AR by inserting within certain genes or in their regulatory regions, thus inactivating/activating/up-regulating certain genes encoding for drug intake, efflux pumps, or activators/inhibitors of some metabolic pathways, which ends up in AR, possibly in multidrug-resistance phenotype.

We propose here an unique strategy to precisely determine the insertion sites of some transposons involved in AR at whole-genome level, by optimizing an inverse-PCR protocol targeted for bacterial ISs. As we will use clinical strains of Klebsiella pneumoniae (Gram negative enterobacteria), we expect to describe the genetic background of resistance determined by transposons for high-risk epidemic clones, and thus to contribute (at international level) to the understanding of resistance spreading in such clones. Also, this work will be materialized in an molecular kit for mapping the insertion sites of AR determining transposons, as a ready-to-use methods for researchers everywhere.

IN SILICO AND EXPERIMENTAL STUDIES OF MOBILE GENETIC PLATFORMS ENCODING FOR MULTI-DRUG RESISTANCE Call name: P 3 - SP 3.1 - Proiecte de mobilități, România-China (bilaterale)
PN-III-P3-3.1-PM-RO-CN-2018-0147 2018 - 2019

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); West China Hospital, Sichuan University (CN)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://bios.unibuc.ro/

Abstract:

Carbapenems (Car) and colistin (Col) are two of the last-resort antibiotics for which clinical usage is threatened by the emergence of antibiotic resistance (AR). The aim of the project is to elucidate some particular aspects regarding the molecular epidemiology of AR of Car and Col in clinical strains of enterobacteria with MDR phenotype in Romania (RO) and China (CN), using techniques of microbiology, genomics and bioinformatics of DNA and protein sequences. The complementary teams, that are collaborating constantly from 2013, will initially perform a screening (Vitek II, Maldi-Tof, PCR, 16S RNA sequencing, ARG resistance genes) for the selection of strains with significant AR profiles (RO, CN) followed by the identification of the Genetic resistance platforms of Car and Col. Some ARGs of Car and all the ARGs of Col identified (RO, CN) will be sequenced and annotated accordingly. The genome of Col resistant strains at which the genetic substrate of the AR could not be identified will be sequenced in order to identify new ARG candidates of Col (CN). The transfer capacity and ARG localization will be assessed by conjugation or electroporation (RO, CN). The plasmids will be analyzed by PBRT and RFLP, and the relevant ones will be isolated and analyzed by WGS (CN). Mutations in blaNDM genes and mutant proteins involved in Col resistance will be analyzed by protein modeling approaches to investigate their effect on conformations and the antibiotic inactivation rate.

Rapid flow cytometric method for microbial detection and antibiotic susceptibility assay directly from clinical specimens Call name: P 4 - Proiecte de Cercetare Exploratorie
PN-III-P4-ID-PCE-2016-0921 2017 - 2019

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://icubresearch.wordpress.com/2018/09/20/pce_2179-2017/

Abstract:

Antibiotics have a major contribution to the decrease of mortality and morbidity caused by bacterial diseases, but unfortunately, due to their misuse or overuse, they favored the emergence and selection of resistant bacteria. The antibiotic resistance (AR) in bacterial pathogens has become a key issue affecting public health, at both national and international level, considering the very limited number of new antibiotics development and the forecast of the “post antibiotic era”. This evidence underlines the need to recognize the problems contributing to poor outcomes and high costs and the need for a multidisciplinary effort to combat resistance. The most important is the adequate selection of the initial (empiric) antibiotic therapy before susceptibility test results become available. Speed is essential when one deals with bacterial infections. Severe or nosocomial infection are usually treated empirically with broad-spectrum antibiotics. If the identity and resistance profile of the microorganism could be determined simultaneously with biochemistry and haematology results, antibiotic prescription rates could go down dramatically. Speed also improve the targeted treatment and the isolation of patients carrying multidrug-resistant organisms, preventing the spread of AR. Thus, the availability of a rapid method for the direct detection of resistant bacteria in the clinical sample, much earlier than the susceptibility testing results, would improve this first and foremost step, allowing a proper selection of the empiric (emergency) therapy. The aim of this project is to enhance the potential of flow cytometry to create a cost-effective, accurate, rapid and easy-to-use test for resistance bacterial infections directly from the clinical specimens that will allow health professionals to orient the emergency antibiotic therapy, in order to tackle the growing levels of antimicrobial resistance

Sustainable materials based on zinc oxide for antimicrobial applications Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-0240 2017 - 2018

Role in this project: Partner team leader

Coordinating institution: INSTITUTUL DE CHIMIE FIZICA - ILIE MURGULESCU

Project partners: INSTITUTUL DE CHIMIE FIZICA - ILIE MURGULESCU (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.icf.ro/pr_2017/Biosusmat_2018.pptx

Abstract:

The goal of the present project represents the development of new inorganic biocidal agents toward prokaryotic and eukaryotic microbial strains, in planktonic and biofilm adherent state. Its general objective is the optimizing of the design for zinc oxide based nanomaterials, using eco-friendly synthesis in order to reach an effective antimicrobial activity, and the component/subsystem validation in laboratory environment. The obtained ZnO crystallites lies in the nano range. In these context the specific objectives of the project are:

1. selection of the synthesis procedure in terms of antimicrobial and cytotoxic activity;

2. for the selected synthesis procedure, the optimization of the experimental parameters in terms of antimicrobial and cytotoxic impact;

3. validation in laboratory environment of the antimicrobial and cytotoxyc activities;

4. validation in laboratory environment of the synthetic procedure;

5. dissemination and intelectual property protection.

This project aims to develop an inorganic antimicrobial material from technology maturity TRL 3 to TRL 4. The technology maturity TRL 3 of the already undertaken research is shown by: (i) the concept of ZnO nanomaterials with antimicrobial properties obtained via eco-friendly methods is proved experimentally and its efficiency is verified, (ii) each component of the assembly, namely ZnO as inorganic material, the eco-friendly synthesis of ZnO, and the antimicrobial activity of the resulting materials were tested. The final parameter of interest is the antimicrobial activity of these ZnO materials. According to the proposed objectives, the implementation of the project will lead to the integration of the three components in order to establish the functionality of the assembly, the antimicrobial tests being carried out in suitable conditions in accordance with widely approved methods.

Composite hydrogels based on inorganic nanoparticles and collagen with prolonged antimicrobial activity for the prevention of wound infections Call name: P 2 - SP 2.1 - Proiect de transfer la operatorul economic
PN-III-P2-2.1-PTE-2016-0177 2016 - 2018

Role in this project: Key expert

Coordinating institution: SANIMED INTERNATIONAL IMPEX S.R.L.

Project partners: SANIMED INTERNATIONAL IMPEX S.R.L. (RO); UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://nanocolagel.sanimed.ro/

Abstract:

Chronic wounds represent a good niche for biofilm development because the impaired immune response promotes infection susceptibility whilst necrotic tissue and debris favor bacterial attachment. Collagen hydrogels represent one of the most efficient treatments in case of both chronic and acute wounds due to their ability to maintain optimal humidity and aeration parameters. Currently, at a national level there is no production of collagen hydrogels aimed for the treatment of chronic wounds, hence these types of products are being imported. In this context, the present project proposal aims to design and obtain new types of multifunctional collagen hydrogels harboring antimicrobial properties in order to favor the healing process of chronic wounds. As a novelty element in comparison to products currently available on the international market, we will design and produce collagen hydrogels containing nanoparticles. Thus, Sanimed International Impex S.R.L will develop a simple and rapid technology to obtain hydrogels functionalized with metalic and oxidic nanoparticles (collagen hydrogels with Ag nanoparticles, collagen hydrogels with ZnO hydrogels and collagen hydrogels with SiO2@ZnO nanoparticles). The novel hydrogels will be tested for their antimicrobial and antibiofilm properties using in vitro and in vivo methods and also for the host response after hydrogel treatment on wounded cells and in vivo lesion murine models, for achieving market preparation.

Knowledge Transfer on investigation of the anti-infective and antitumoral activity of novel cosmetic and pharmaceutical formulations based on natural extracts Call name: P 2 - SP 2.1 - Transfer de cunoaștere la agentul economic „Bridge Grant”
PN-III-P2-2.1-BG-2016-0369 2016 - 2018

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO); HOFIGAL EXPORT IMPORT SA (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://icubresearch.wordpress.com/2018/10/19/bg-369/

Abstract:

The present project proposal aims the development of an innovative methodology for testing the antimicrobial, antiviral, anti-tumor and immunomodulatory activity, and to elucidate the mechanisms of action at the cellular and molecular level of natural bee and plant extracts with the purpose of introducing them în new cosmetic and pharmaceutical formulations, for expanding the marketed products range of the economic agent participating în the project - Hofigal S.A.

The addressed strategy is based on abundant scientific literature and original experimental results of all involved partners regarding the characterization and marketing of natural products / compounds with therapeutic or prophylactic value.

The proposed project falls into a major research direction aimed at the discovery of complementary therapeutic solutions based on natural compounds, for the treatment of different pathologies (i.e., infectious diseases and cancer), which are currently found în the top of causes of global morbidity and mortality.

The therapeutic alternatives that will be investigated în this project are based on natural compounds of vegetal and bee origin, with high therapeutic and preventive potential, due to the following properties: complex composition that allows simultaneous action on multiple biological targets; decreased risk for selecting resistance, both în case of infectious agents, such as viral, bacterial and fungal, and în the case of tumor cells; immunomodulatory effect; high biocompatibility; minimal or no side effects.

Increasing performance/competitiveness by improving the technology of smart products fabrication with superior biocompatibility and antibacterial properties for cosmeto-medical applications Call name: P 2 - SP 2.1 - Transfer de cunoaștere la agentul economic „Bridge Grant”
PN-III-P2-2.1-BG-2016-0142 2016 - 2018

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO); SONNENKREUZ SRL (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.unibuc.ro/n/cercetare/94BG-2016.php

Abstract:

The purpose of this project is to increase the performance and the competitiveness of SC Sonnenkreuz SRL by using the expertise existing in the research organizations, University of Bucharest and INCDCP-ICECHIM, for developing and optimizing the modern technology for producing silver colloid dispersions, in order to obtain a product with higher biocompatibility and antimicrobial properties. To accomplish the purpose of the project, a full compliance between the requirements and the problems identified by the economic agent and the project objectives was followed. Two directions for innovating the final product and the manufacturing processes will be pursued: (i) obtaining of various colloid silver dispersions stabilized with polymers having intrinsic antibacterial activity, to achieve a synergistic effect and (ii) optimizing the production technology by electrochemical method by ensuring the control of nanoparticles growth. The knowledge transfer will include also the collaboration with the economic agent in order to identify the possibility of a subsequent development of other cosmeto-medical products based on the dispersions of silver nanoparticles with improved properties. For developing the quality assurance system at the economic agent, the collaboration between the partners UB and INCDCP-ICECHIM with SC Sonnenkreuz SRL will be intensified in order to accomplish the analyzes required for the characterization of the final products and to monitor the manufacturing process. Cooperation with the economic partner will be fulfilled by the activities of entrepreneurship knowledge transfer towards the researchers/PhD students/Master students involved in the project. Development of the new product and improvement of the technology will provide opportunities for practical training for master students, with a complex topic. The publishable scientific results will be widely disseminated in communications and articles in specialized journals.

Contributions to enhancing the Sanimed SRL’s competitiveness by the knowledge assimilation and potential production implementation of some 3D collagen-polymer hybrid matrices intended to tissue repair Call name: P 2 - SP 2.1 - Transfer de cunoaștere la agentul economic „Bridge Grant”
PN-III-P2-2.1-BG-2016-0397 2016 - 2018

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO); SANIMED INTERNATIONAL IMPEX S.R.L. (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.unibuc.ro/prof/micut_m/

Abstract:

The project proposal mainly aims at knowledge transfer to economic operator regarding the obtaining and development of 3D collagen-polymer hybrid biomaterials intended to biomedical applications. The practical implementation of the proposal should lead to enhancing competitiveness of private economic entity in order to develop itself in the near and middle-term future. In line with economic partner, the public entities University of Bucharest and ICECHIM (project partners) have identified aspects of scientific and technological existing at private partner, the weaknesses and strengths of these aspects, as a useful basis for designing new collagen-based hybrid biomaterials. In addition, the purpose that such kind of biomaterials be used in the process of tissue repair requires accounting a number of key characteristics against which it is possible to design, realize and develop this kind of hybrid collagen-polymer system: biocompatibility, bioresorbability, mechanical properties, internal and external morphology and manufacturing technology.

Innovative surface design to extend the life and safety of dental and orthopedic implants Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-0910 2017 - 2018

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte/proiecte-cu-finantare-nationala/ped-97-2017/

Abstract:

NovSurf addresses a critical health issue, namely the increasing incidence of dental and orthopedic implant revisions due to implant associated infection and inflammation, by proposing a novel approach based on development of a surface topography of nanochannelar type on titanium implants by anodization, on which zinc oxide nanoparticles will be incorporated as antimicrobial agents. The newly designed surface is expected to reduce the susceptibility of titanium implants to microbial colonization, to enable the decontamination of implants if infection occurs and to support bone healing around the implant. In-depth characterization of physical properties, chemical composition and biological activity of modified titanium surface is envisaged. In vitro studies will evaluate its antimicrobial and anti-biofilm properties, its bioactivity, and inflammatory potential. Preliminary in vivo evaluation of the extent of bone regeneration after insertion in rats femur of surface modified titanium pins will provide efficacy data insuring the proof of concept by the end of the project. The project is built on two pillars of expertise afferent to the institutions involved, namely the strong background in the electrochemical deposition of TiO2 nanostructures on implant materials and their characterization at University Politehnica of Bucharest, and the excellent capabilities in assessment of microbial and cell-biomaterial interactions at University of Bucharest. The team gathers researchers with complementary expertise acting in a synergistic manner in order to solve an important health issue related to implant failure. If our expectation will be fulfilled, the findings may form a good foundation for further potential clinical validation of Ti-based implants with this innovative surface design as ready products for clinical applications.

Development of functional model of microbial fuel cell for bioelectricity production with simultaneous municipal wastewater treatment Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-1008 2017 - 2018

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://sites.google.com/a/psg.unibuc.ro/fm-mfc

Abstract:

Microbial fuel cells (MFCs) are an emergent and a promising technology which converts organic matter into electricity with the help of exoelectrogenic bacteria present in the wastewater.

The scope of this project is to combine the results generated in current research, our research results on microbial fuel cells and interdisciplinary expertise of the research team and apply them to design a functional model of MFCs for treating municipal wastewater and generate bioelectricity in the laboratory environment. The minimization of the problems associated with the performance of MFC could be achieved by an effective electron transfer from microbes via anode which involves the high electrical conductivity of the anode materials and enhancing the surface area, porosity and biocompatibility of the anode. Furthermore, the availability of the anode materials is an essential requirement for MFC commercialization.

Our approach to improve the MFCs performance and for the development of a functional model of MFCs refers to the improvement of the electrode design , which is considered the biggest challenge in achieving a scalable and cost efficient MFCs technologies. Addressing this challenge, modification of the anode with advanced carbon based materials (such as CNTs, graphene and their composite with conducting polymers) will be used to improve planar and 3D configuration of MFCs anodes together with biofilm investigation and biocompatibility assay. Different MFC electrodes and reactor configurations will be tested for the improvements in power generation of MFCs and wastewater treatment in order to develop a functional model of MFCs.

Magnetite nanoparticles functionalized with natural compounds to modulate Pseudomonas aeruginosa virulence and persistence Call name: Projects for Young Research Teams - RUTE -2014 call
PN-II-RU-TE-2014-4-2269 2015 - 2017

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://alina.amgtranscend.org/?page_id=44

Abstract:

Nowadays, about 70 percent of the bacteria that cause infections in hospitals but also in communities are resistant to at least one of the drugs most commonly used for treatment, causing infections that often fail to respond to standard therapy. Most resistant microorganisms in Romanian hospitals are the Gram negative non-fermenting strains of P. aeruginosa. The aim of this project is to investigate the effect of bioactive magnetite nanoparticles functionalized with plant-derived compounds on the virulence and persistence of some laboratory and clinically isolated, resistant P. aeruginosa strains. Our innovative approach is using subinhibitory concentrations of plant-derived compounds to modulate microbial behavior and virulence, rather than induce their killing, in order to limit the development and spread of resistant mutants, since strategies that interfere with bacterial population fitness are selecting resistant mutants. Knowing the intimate mechanisms of action of these bioactive nanoformulations with proved antimicrobial effect on the P. aeruginosa cells will significantly improve knowledge and offer further perspectives in developing alternative preventing and treatment approaches of this pathogen. This project is expected to have a great potential not only on the research field but also on the socio-economic sector, since it aims to develop an alternative method to solve or attenuate a global problem, as is the antimicrobial resistance.

RISK ASSESSMENT OF TRANSPOSON-MEDIATED TRANSFER OF SOME CARBAPENEMASE GENES Call name: Projects for Young Research Teams - RUTE -2014 call
PN-II-RU-TE-2014-4-2037 2015 - 2017

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.unibuc.ro/n/cercetare/icub/stiintele-vietii-mediului-si-ale-pamantului/Proiecte_de_cercetare_derulate_prin_ICUB0.php

Abstract:

Gram negative bacilli (GNB) resistance to carbapenems represents a global public health issue, due to severe, hard to treat infections, with high rates of morbidity and mortality.

In Romania, there was previously described by our research team the high prevalence of carbapenemase encoding genes (CRG) like NDM-1, OXA-48 and OXA-181 in enterobacterial strains, VIM-1, VIM-2 in Pseudomonas aeruginosa and IMP-1, OXA-23 and OXA-51 in Acinetobacter baumannii strains. The molecular mechanisms mediating the CRG transfer are not well investigated.

The purpose of this project is designing and developing molecular protocols with the contribution of molecular biology, genetics and bioinformatics, for the assessment of some CRGs from Enterobacteriaceae, P. aeruginosa and A. baumannii transfer risk, by establishing the transposon-mediated mobilization potential of CRGs, that will make possible the identification of high-risk clones and isolation of critical outbreak sources.

The approach of this project is innovative because it is based on testing the genetic environment of resistance (the sequence of CRG alleles, their molecular function and the required mobilization context), which will contribute to establish some molecular criteria for the evaluation or the CRGs risk level (the CRG gene/allele have mutations that can increase the efficiency of the carbapenemase, the allele is overexpressed and it is framed by active transposable elements).

Innovative nanostructured materials and coatings with antimicrobial activity for medical applications Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-1292 2014 - 2017

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU INGINERIE ELECTRICA ICPE - CA BUCURESTI

Project partners: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU INGINERIE ELECTRICA ICPE - CA BUCURESTI (RO); UNIVERSITATEA BUCURESTI (RO); MGM STAR CONSTRUCT S.R.L. (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.icpe-ca.ro/proiecte/proiecte-nationale/pn-2014/inmatco.pdf

Abstract:

The project aims to develop novel products and knowledge in antimicrobial nanostructured coatings for medical applications like critical surgical instruments (scalpels, knives, forceps and scissors) to reduce superficial and deep surgical site infections and to improve quality of life. Nanostructured materials of MeNPs/MeONPs type (MeNPs = Ag, Cu, MeONPs = CaO, MgO, ZnO, TiO2) with average MeNPs size of 10-20 nm and average MeONPs size of 30-50 nm) and high antimicrobial activity will be obtained by an innovative chemical synthesis of colloidal suspensions containing ecological NPs with 0.1-0.5 wt.% MeNPs and 1-3 wt.% MeONPs. Chemical deposition of MeNPs on MeONPs supports will be realized by a self-assembled nanolayers coating process, being a new and efficient approach in control of NPs size and stability. A higher content of MeNPs will be obtained in comparison with the existent solutions. The MeNPs and MeONPs mixtures will show synergistic effects of antimicrobial properties compared with those of MeNPs and MeONPs used alone. Ecological and efficient processing methods will be used to obtain and deposit the bioactive agents designed to inhibit the adhesion of microorganisms on stainless steel supports that show lack of antibacterial properties. The MeNPs/MeONPs composite powders will be deposited by different methods such as Thermal Evaporation, EBPVD (e-gun), Sputtering Deposition (Magnetron) and Arc-PVD (Plasmotron) on the metallic substrates with ensuring a good adhesion without changing the substrates original properties. Nanostructured coatings with homogenous and dense surface without any faults (pinholes and cracks) will be achieved at low temperatures to not affect the materials properties. Optimized coatings will be developed by a graded structure and adjusting stress level. Other original and innovative aspect is related by sputtering targets development from selected nanostructured materials by spark plasma sintering (SPS) process, where nanostructure features are maintained since grain growth and the development of equilibrium states are suppressed. The project addresses to the priority thematic of the PARTNERSHIPS PN-II-PT-PCCA-2013-4 program, Domain 7. “Materials, processes and innovative products“, Research field 7.1 Advanced materials, Thematic Area 7.1.6. “Advanced materials and biomaterials for health quality increasing“ with a direct impact in Domain 4. “Health” and European Research Area (ERA)-Nanomaterials. The project has inter-disciplinary, multi-disciplinary and trans-disciplinary nature being carried out by high skilled specialists with complementary expertise. Certified prototypes and end-products of critical stainless steel surgical instruments coated with antimicrobial MeNPs/MeONPs nanostructured materials with uniform and homogeneous mono/multilayer with thickness ranging 0.1-50 µm will be obtained. The coatings will have high resistance to mechanical wear, high resistance to heat stresses, high hardness, low friction coefficient and good adhesion to substrate. The novel products will be introduced on the market only after their certification granted by the Health Ministry from Romania. The coated products will be at a competitive cost with uncoated ones, contributing to the requests of the global and national socio-economic environment in the field of advanced materials and health with a major impact in reducing surgical site infections. Knowledge protection will be made by two patent applications registered to the national State Office for Inventions and Trademarks (OSIM). Results dissemination will be made on a large scale by publishing scientific papers in ISI ranked journals, attendance at national and international events, setting up and updating project web site, elaboration of dissemination materials.

Bioceramic composites with local applications in antibacterial therapy Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0005 2014 - 2017

Role in this project: Partner team leader

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA

Project partners: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); SPITALUL CLINIC DE URGENTA (RO); UNIVERSITATEA BUCURESTI (RO); BIOSINTEX SRL (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.infim.ro/ro/projects/compozite-bioceramice-cu-aplicatii-locale-terapia-antibacteriana

Abstract:

In recent years, much attention has been paid to the synthesis and characterization of nanomaterials because of their interesting properties, which mainly come from the high surface/volume ratio. Hydroxyapatite incorporated with antimicrobial agents is able to prevent or cure infections by releasing directly the antimicrobial agents to local regions. HAp incorporated with Ag/Ag+, Cu2+/Zn2+, ampicillin and doxycycline have been studied to evaluate their antibacterial effect. Among those, Ag incorporation has gained more attention due to its broad-spectrum antibacterial properties. On the other hand in the latest years, the development and the use of antimicrobian agents in treating acute or chronic cutaneous lesions reached a development from the stage of clinical research to the appearance of some commercial products series.

Our project is based on a multidisciplinary approach involving physics, chemistry, microbiology, human toxicology and medicine. The project objectives are to highlight the major contributions that could be made by nanoengineered particles of hydroxyapatites for biomedical applications such inhibition and prevention of bacterial infections. The project will test methods to synthesis silver doped hydroxyapatite and collagen/silver doped hydroxyapatite able to inhibit the bacterial activity of different species of bacteria in the laboratory that will be implemented in real infected sites.

The decisive aim of this project is to develop specific nanoparticles/composites to prevent and remediate bacterial infections, without using antibiotics. For this reason, the new biomaterial based on nanoparticles of hydroxyapatite doped with silver and collagen/silver doped hydroxyapatites composites,will have antimicrobial properties due to their characteristics, but there is no risk to develop bacterial resistance.

Integrated system for biomolecular modeling, with applicability to the study of the Gram-negative bacteria Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-2087 2014 - 2017

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA SI INGINERIE NUCLEARA " HORIA HULUBEI " - IFIN - HH

Project partners: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA SI INGINERIE NUCLEARA " HORIA HULUBEI " - IFIN - HH (RO); UNIVERSITATEA BUCURESTI (RO); TOTAL SOFT S.A. (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://simbagran.ifin.ro/

Abstract:

SIMBAGraN aims at developing a versatile software tool for computational modeling at molecular level, and its use in the investigation of Gram-negative bacteria. The system is adaptable for the investigation of various subcellular structures, allows the upgrade to arbitrarily high capacities for processing and storage of data, and is scalable with the size of these capacities and the number of users. These features are implemented through a multifunctional web portal connected to a computing grid composed of high-performance clusters. The portal integrates various software tools accessible through a graphical interface which allows the definition and use of workflows composed of tasks / modules that correspond to various modeling steps. The workflows are submitted for execution on the available resources of the grid system in a way which is transparent to the user.

Although the integrated system allows the investigation of various topics in computational biology, it will initially be customised and used for the modeling and simulation of Gram-negative bacteria. The choice of this topic is motivated by the national priority to investigate the vulnerabilities of drug-resistant bacteria that are responsible for many hospital-acquired infections in Romania, and whose understanding can result in better prevention and treatment methods for life-threatening morbidities. The project will empower the microbiology and pharmacology researchers with software tools allowing them to perform faster and systematic numerical simulations of the drug-bacteria interactions for the bacterial strains specific to Romanian hospitals.

The integrated system is designed such as to become a central node for the national biocomputing community, through the gradual connection to grid of the resources for computational biology which are hosted in Romanian universities and research institutes. This process will be favoured by the obvious advantage of using the innovative services offered by the integrated system such as, for instance, modeling workflows for bacterial strains.

The portal is intended to become in time a node of the European infrastructure for biological information - ELIXIR, operating national and institutional databases, storing bioinformatics data and providing computational resources and services for large scale modeling. The national node will also provide a repository and expertise for newly created data and software tools. Through the portal, the international community will have access to data specific to Romania (e.g. of interest for microbiology and pharmacology), and Romanian researchers will benefit on the large scale dissemination of their scientific results. This will substantially contribute to bridging the technological gap between the current ELIXIR groups and the national research community, whose international visibility will increase significantly.

The user community of the prototype of the national grid for computational biology will include researchers, master and PhD students from Romania and abroad.

The main end-product of SIMBAGraN - the prototype of the bioinformatic system - will provide innovative services to this community, and will be tested during the project, delivering publishable scientific results regarding the structure and functionality of the membranes and proteins of Gram-negative bacteria. Also, it will provide an excellent e-learning environment for students enrolled in molecular biology programs, allowing them (controlled) access to various databases, drawing and visualization tools, means for storing, sharing and reusing session's results, quizzes, etc.

Innovative antibacterial and self-cleaning photocatalytic textiles Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0419 2014 - 2017

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU TEXTILE SI PIELARIE - INCDTP BUCURESTI

Project partners: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU TEXTILE SI PIELARIE - INCDTP BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA (RO); UNIVERSITATEA BUCURESTI (RO); STOFE BUHUSI S.A. (RO); C & A COMPANY IMPEX SRL (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://cleantexproject.ro/

Abstract:

The negative impact of pollution on human health and environment pushed the R&D efforts to develop clean technologies and products according to the green chemistry principles, making regulation, control, clean-up and remediation unnecessary.

The project’ target is the development of new multifunctional textiles providing simultaneously photocatalytic, auto-sterilizing, self-cleaning and enhanced antimicrobial properties based on innovative graphene oxide/TiO2 nanocomposites able to decompose pollutants in safe, non-toxic compounds, using only solar light.

The present project aims at developing the 4th generation of green photocatalysts by:

• synthesis of graphene oxide/doped titanium oxide (GOT) with efficient absorption under UV and visible light

• formulation of GOT composites as highly adherent solution;

• development of photocatalytic textiles by deposition of photocatalytic compounds in one-step technology;

• evaluation of photocatalytic/self-cleaning/antimicrobial performances of synthesized photocatalysts and textiles against usual pollutants and pathogenic microorganisms,

• biocompatibility/cytotoxicity testing of cells cultures toward photocatalytic compounds.

The original contribution of the project consists in:

• optimal assembly and interfacial coupling of the TiO2 nanoparticles over the graphene oxide sheets;

• innovative adhesive graphene oxide/TiO2 (GOT) formulations, ensuring a high and stable dispersion and a strong adherence of the composites to the textile substrate, while preserving the genuine physical and mechanical properties of textile;

• homogeneously and firmly adherent photocatalytic coatings by one step deposition of GOT at room temperature, reducing the raw materials, utilities and manpower consumption;

• new investigations on graphene/TiO2 cytotoxicity and biocompatibiliy.

The indicators proposed to be achieved:

- eco-friendly products: minimum 2 types of composites nanopowders; min. 2 types of textile materials with high photocatalytic and antibacterial efficacy; self-cleaning and antibacterial work wear and protective equipment; clean technologies: one step synthesis of powder composites; one step deposition and fixation of synthesized compounds on textiles;

- innovations: 1 patent describing the innovative GOT synthesis and one-step deposition of nanocomposites; minimum 2 ISI rated scientific papers, minimum 2 presentations at national and international conferences.

The innovative approach for the production of high quality photocatalytic textiles is based on an efficient, environmentally clean and easily implementable at industrial-scale process, one step deposition and fixation of environmentally friendly nano composites. This finishing technology combined with a proper adhesive photocatalysts formulation will eliminate the post-treatment steps, and consequently high consumption of chemicals, water and energy and will allow the achievement of highly adherent, durable, uniform coating thin layers preserving the genuine physical properties and colour of the textiles. The easy application on conventionally existing production lines, ensure a wide spread of the finishing technology. The approval of the present project will contribute to concentration of human and material resources with the aim to achieve the above mentioned research tasks and the implementation of results in Romania, with special amelioration of the economic situation of SMEs active in textiles and chemical industry and significant improvements of human health and environment quality.

Effects of P. aeruginosa quorum sensing molecules on Drosophila genome: a new tool to identify candidate genes involved in host-pathogen crosstalk Call name: Exploratory Research Projects - PCE-2011 call
PN-II-ID-PCE-2011-3-0576 2011 - 2016

Role in this project:

Coordinating institution: UNIVERSITATEA DIN BUCURESTI

Project partners: UNIVERSITATEA DIN BUCURESTI (RO)

Affiliation: UNIVERSITATEA DIN BUCURESTI (RO)

Project website: http://microbiologyresearch.wordpress.com

Abstract:

Pseudomonas aeruginosa, an emerging multi-drug resistant organism, one of the major contributors to morbidity and mortality in opportunistic and nosocomial infections, communicates intra-species and with the eukaryotic hosts by cross-kingdom inter-cellular signaling mechanisms such as quorum sensing (QS), relying on low-molecular weight excreted molecules, to control the production of virulence factors. The P. aeruginosa QS signaling molecules (QSSMs) have in vitro pleiotropic effects on eukaryotic cells. In the present project we propose an original, integrative in vivo approach for the investigation of the way in which such signaling molecules modulate the Drosophila melanogaster genome, in order to identify the most sensitive eukaryotic genes targeted by the QSSMs and the specific orthologous genes in the Homo sapiens genome. In order to accomplish our purpose, we will evaluate the phenotypic effects of QSSMs and viable P. aeruginosa cells (virulent versus quorum sensing defective strains) on D. melanogaster wild type and mutant strains, highlight the genes expressing a significant up- or down-regulation, identify by bioinformatics the orthologous human genes, assess the effects of P. aeruginosa QSSMs and viable P. aeruginosa cells on the respective genes profiles in the human eukaryotic cells and finally formulate the clinical contribution of our findings to future development of novel therapeutics for a multitude of infectious and immunological diseases.

Selection of novel functional lactic acid bacteria from plant origin materials, with potential applications in food biotechnology Call name: Joint Applied Research Projects - PCCA-2011 call, Type 1
PN-II-PT-PCCA-2011-3.1-0621 2012 - 2016

Role in this project: Key expert

Coordinating institution: INSTITUTUL DE BIOLOGIE

Project partners: INSTITUTUL DE BIOLOGIE (RO); UNIVERSITATEA BUCURESTI (RO); ASOCIATIA CENTRU DE CERCETARE STIINTIFICA IN DOMENIUL BIOCHIMIEI APLICATE SI BIOTEHNOLOGIEI (BIOTEHNOL) (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.ibiol.ro/proiecte/PNII/PLANTLAB

Abstract:

Food safety and improving the food quality are two major concerns worldwide, since food is an important part of life. Consumers pay nowadays more attention to the relation between food and health, but they also ask for more diversity, for foods with better taste and more appealing, minimally processed, without chemical preservatives, but still safe and able to maintain good shelf life. In this context, our project aims to isolate new lactic acid bacteria from plant-origin materials (poorly explored niches in Romania - fresh fruits and vegetables, flowers, fermented vegetables and fermented cereals), in a search for novel functional strains, with potential applications in food biotechnology. The main result of the project will be an important collection of LAB strains, identified and characterized, with an associated database containing the profiles of all microorganisms isolated and identified, but also their functionality, which might be also exploited in other further projects. A special interest will go to the selection of LAB strains with antifungal/antibacterial activity towards foodborne pathogens or spoilage microbiota, with potential use as biocontrol or biopreservation agents. LAB strains with other functional properties (production of bacteriocins, exopolysaccharides, vitamins, potential probiotic effect) selected in this project, will be included in functional starter cultures to be used for the production of standardized fermented vegetables and fermented cereals with high nutritional value, superior sensory properties and hygienic safety. The metabolites involved in some of the functional aspects (for instance bacteriocins and exopolysaccharides) will be isolated, purified and characterized, as a first step for a possible further use in food industry.

Inovative, multidisciplinary research to investigate the probiotic effects of new lactic acid bacteria strains and consortia Call name: Joint Applied Research Projects - PCCA-2011 call, Type 1
PN-II-PT-PCCA-2011-3.1-0969 2012 - 2016

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL DE BIOLOGIE (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); UNIVERSITATEA DE STIINTE AGRONOMICE SI MEDICINA VETERINARA (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://genetmicroproiecte.blogspot.ro/search/label/PROLAB%2077

Abstract:

The emergence of bacterial resistance and multiresistance to antibiotics represent a major public health problem in Romania and also in the entire world. In Romania, there is an increasing trend of the incidence of infections with bacterial strains highly resistant to antibiotics, whose prognosis is often worsened by the producing of bacterial biofilms on the tissues and biomaterials used in medicine. In our country, the problem of development of some alternative strategies for the prevention and treatment of the infections with multiresistant and biofilm forming bacteria is less studied, despite their great application potential in biomedicine and their utility in the recovery of the balanced or eubiosis state of the normal microbiota, after the negative impact of the antibiotics on bacterial communities. One of the most viable alternatives is represented by probiotics products which by administration in adequate amounts confer a health benefit on human organism. For the best use of the probiotic microorganisms, the mechanisms by which they work should be better understood. The selection of an appropriate probiotic strain for its inclusion in a probiotic preparation should be made on the basis of its capacity to induce an improved host response without modification of the intestinal homeostasis.

The main goal of this project imply complex studies of probiotic strains (lactic acid bacteria newly isolated) and multispecific consortium in order to improve the experimental protocol for the optimal selection of human use probiotics, based on their anti-infective and immunomodulatory activities.

Novel nanostructured prosthetic tubular devices with antibacterial and antibiofilm properties induced by physico-chemical and morphological changes Call name: Joint Applied Research Projects - PCCA-2011 call, Type 2
PN-II-PT-PCCA-2011-3.2-0284 2012 - 2016

Role in this project: Partner team leader

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); I.C.P.E. BISTRITA S.A. (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.antibiotube.ficai.eu

Abstract:

The project is intended to overcome the bacterial colonization of the prosthetic tubular devices, this being one of the most common reason of the tubular devices failures. The main objectives of the project include the surface modifications of the existing prosthetic tubular devices but, also bulk modifications will be tested, especially from the point of view of drug release properties. As surface modifications techniques, chemically modifications will be tested because it allows strong interactions between substrate and coating. Based on the existing expertise of P1-INCERPLAST, the only Romanian prosthetic tubular devices manufacturer, the PVC based devices will be used as substrate. The surface modifications will involve the partial substitution of Cl with HO followed by different chemical reaction: 1) alkylation/acylation when hydrophobic groups are intended to be attached on the surface of the tubular devices (in this way, the adhesion of bacteria is not favored); 2) thin polyurethane or silicon layer deposition starting from synthesized prepolymers, the withdrawal reaction being between prepolymer and hydroxyl groups of partially substituted PVC support; 3)coating of the tubular devices surfaces with antimicrobial nanoparticles, the stability of the coating being improved by proper functionalization of the substrate (if nanoAg are intended to be deposited on the PVC tubular devices, chloride will be partially substituted with SH; 4) the tubular prosthetic devices will be impregnated with antimicrobial/antibiofilm/anti-fouling agents, chemical bonding being prefered; and 5) new tubular devices will be obtained, the wall acting as a support for drug delivery. All these devices will be carefully selected and will be the subject of various scientific publications and patents. The ultimate goal of the project is the CE certification and finally their commercialization.

MOLECULAR ANALYSIS OF PROBIOTIC STRAINS ANTIMICROBIAL ACTIVITY MECHANISMS AGAINST UROGENITAL INFECTIOUS AGENTS Call name: Projects for Young Research Teams - TE-2010 call
PN-II-RU-TE-2010-0124 2010 - 2013

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DIN BUCURESTI

Project partners: UNIVERSITATEA DIN BUCURESTI (RO)

Affiliation: UNIVERSITATEA DIN BUCURESTI (RO)

Project website: https://sites.google.com/site/pelinescute66, http://genetmicroproiecte.blogspot.ro

Abstract:

UROGENITAL INFECTIONS FREQUENCY, HIGH DEGREE OF RECURRENCE AND THE EXTENT OF MICROORGANISM’S ANTIMICROBIAL RESISTANCE TO ANTIMICROBIAL COMPOUNDS IMPLY SEARCHING FOR SOME ALTERNATIVE STRATEGIES FOR THE PREVENTION AND TREATMENT OF THE INFECTIONS. A VIABLE SOLUTION IS REPRESENTED BY THE USE OF PROBIOTICS BASED ON LACTIC ACID BACTERIA STRAINS. UNDERSTANDING OF MECHANISMS INVOLVED BOTH IN THE EXPRESSION OF VIRULENCE AND PATHOGENICITY IN MICROBIAL STRAINS (BACTERIA AND FUNGI), AND PROBIOTICS WAY OF ACTION MAY GENERATE THE DEVELOPMENT OF ALTERNATIVE TREATMENT STRATEGIES. THE MAJOR OBJECTIVE OF THE PROJECT WE PROPOSE CONSISTS IN STUDIES ON MOLECULAR MECHANISMS INVOLVED IN ANTIMICROBIAL ACTIVITY OF LACTIC ACID BACTERIA AGAINST PROKARYOTIC AND EUKARYOTIC MICROORGANISMS, ETIOLOGICAL AGENTS OF UROGENITAL INFECTIONS. COMPETENT RESEARCH TEAM MEMBERS AND THE EXISTENCE OF MODERN INFRASTRUCTURE IN MICROGEN, WILL ENABLE ACHIEVEMENT OF SCIENTIFIC ACTIVITIES PROPOSED BY THE APPLICATION OF MICROBIOLOGY, GENETICS AND MEDICAL BIOLOGY ADVANCED TECHNIQUES. THE RESULTS WILL LEAD TO OBTAINING IMPORTANT THEORETICAL AND PRACTICAL DATA BY CREATING A COLLECTION OF PROBIOTIC LACTIC ACID BACTERIA AND PATHOGENS (BACTERIA AND YEAST) NEWLY ISOLATED, IDENTIFIED BY MODERN POLYPHASIC TAXONOMY TECHNIQUES AND CHARACTERIZED IN TERMS OF VIRULENCE AND PATHOGENICITY TO GENE EXPRESSION LEVEL, IDENTIFICATION OF MECHANISMS INVOLVED IN LACTIC ACID BACTERIA ANTIMICROBIAL ACTIVITY AND CAPACITY TO REDUCE EXPRESSION OF VIRULENCE AND PATHOGENICITY FACTORS IN BACTERIA AND YEAST STRAINS, ETIOLOGICAL AGENTS OF UROGENITAL.

THE CORRELATION OF PSEUDOMONAS AERUGINOSA AND STAPHYLOCOCCUS AUREUS VIRULENCE AND/OR RESISTANCE PATTERN WITH THE CLINICAL OUTCOME OF NOSOCOMIAL INFECTIONS Call name: Projects for Young Research Teams - TE-2010 call
PN-II-RU-TE-2010-0135 2010 - 2013

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA DIN BUCURESTI

Project partners: UNIVERSITATEA DIN BUCURESTI (RO)

Affiliation: UNIVERSITATEA DIN BUCURESTI (RO)

Project website: http://microbiologyresearch.wordpress.com/

Abstract:

Staphylococcus aureus and Pseudomonas aeruginosa are two of the most frequently isolated bacteria in nosocomial infections, being opportunistic pathogens responsible for serious infections in immunocompromised patients. The emergence of extended antibiotic-resistant S. aureus and Ps. aeruginosa strains has made it necessary to develop new strategies to understand the different mechanisms used by these bacteria at the different stages of the infectious process. This knowledge will provide us with the tools to prevent and at least improve the control of S. aureus and Ps. aeruginosa infections. Many studies showed the

involvement of virulence factors in different type of infections, but clear correlations between a certain virulence profile and the initial severity and outcome of clinical infections are still missing. The purpose of the present project is to characterize the virulence and resistance profiles and correlate them with the clinical patterns of nosocomial infections produced by S. aureus and Ps. aeruginosa isolated in the intensive care units (ICUs) in Romania, in order to establish the associations between the presence of putative virulence genes and the outcome of infections caused by these bacteria and to investigate the fundamentals of the epidemiology of new highly virulent resistant multiresistant strains. In this purpose, we will use a complex,

phenotypic and molecular approach in order to detect the genes encoding for different cell-wall associated and extracellular virulence factors, the resistance markers as well as the quorum sensing systems and gene regulators. This is the first scientific attempt to harmonize complex phenotypic and genotypic laboratory methods in order to establish some virulence and resistance profiles of nosocomial agents that could be further used for clinical diagnosis, epidemiological investigations, or routine surveillance of nosocomial infections.

Consolidarea capacității instituționale a Universității din București pentru creșterea competitivității și vizibilității rezultatelor cercetării, în sincronie cu standardele și provocările actuale Call name: Fondul de Dezvoltare Instituţională - FDI - Competiţia 2023
CNFIS-FDI-2023-F-0690 2023 -

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation:

Project website:

Abstract:

Proiectul vizează continuarea demersului susținut prin proiectele FDI anterioare, de dezvoltare integrată a procesului sistemic de cercetare performantă, în domenii de cercetare interdisciplinară și emergentă, la nivelul UB și al partenerilor naționali, dar în colaborare sincronă cu instituțiile de CDI performante din străinătate. Se au în vedere dezvoltarea infrastructurii materiale și de resurse umane, comunicarea rezultatelor cercetării, precum și activități integrative, tip bridge, între UB, CIVIS și mediul academic din UE și SUA. Proiectul vizează trei componente strategice: 1) completarea infrastructurii proprii și modernizarea acesteia, cu o componentă distinctă legată de finalizarea platformelor de înregistrare și raportare a rezultatelor cercetării și de inventariere și popularizare a infrastructurii din UB; 2) dezvoltarea cadrului de colaborare cu partenerii interni și externi prin susținerea activităților de networking (e.g., organizarea/ participarea la evenimente și activități de specialitate); 3) dezvoltarea strategiei de resurse umane pentru cercetare, care să includă toate categoriile de personal CDI, dar și mecanismul, procedurile și protocoalele de interacțiune, colaborare și funcționare sistemică a acestora (inclusiv prin finalizarea demersului de obținere a certificatului HRS4R-Human Resources Strategy for Research, facilitarea accesului la baze de date, suport pentru diseminarea și comunicarea rezultatelor cercetării către mediul academic și societate).

Consolidarea cadrului structural, administrativ și logistic de dezvoltare a unor noi programe de cercetare interdisciplinară în domenii prioritare în cadrul Universității din București Call name: Fondul de Dezvoltare Instituţională - FDI - Competiţia 2022
CNFIS-FDI-2022-0675 2022 -

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation:

Project website:

Abstract:

Prin obiectivele sale, acest proiect vizează, în continuarea demersurilor inițiate prin intermediul proiectelor FDI precedente, consolidarea și dezvoltarea unor direcții de cercetare interdisciplinară, integrativă, complexă în cadrul UB, care se circumscriu primei din cele șase mari ținte strategice ale CE - Mediu și climă - pentru 2020-2024 și respectiv primului hub de cercetare activat în cadrul CIVIS - Climat, energie, mediu, pentru care se așteaptă contribuții științifice și soluții derivate din activitățile de cercetare și inovare din universități.

Ob. 1. Dezvoltarea capacității instituționale pentru cercetare de excelență la UB prin optimizarea infrastructurii de cercetare, perfecționarea resursei umane și dezvoltarea instrumentelor informatice de înregistrare și raportare a rezultatelor cercetării și de digitalizare a patrimoniului muzeal al UB.

Ob.2. Consolidarea sau inițierea unor programe pilot de cercetare pe termen lung, care să utilizeze infrastructura stațiunilor de cercetare ale UB și să contribuie la integrarea acestora în infrastructuri europene de cercetare (LifeWatch, eLTER, ICOS).

Ob. 3. Susținerea activităților de networking, diseminare și comunicare a rezultatelor cercetării către societate prin organizarea de evenimente științifice, vizite de lucru, sprijinirea publicării rezultatelor cercetării în regim open science și dezvoltarea componentei de comunicare pe scară largă a informației științifice și de promovare a cercetătorilor în societate.

Creșterea competitivității și vizibilității instituționale a UB: De la dezvoltarea modelului colaborativ, a resursei umane și a infrastructurii specifice la diseminarea rezultatelor cercetării Call name: Fondul de Dezvoltare Instituţională - FDI - Competiţia 2021
CNFIS-FDI-2021-0405 2021 -

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation:

Project website:

Abstract:

Identificarea și selecția obiectivelor s-a realizat în acord cu nevoile UB de a asigura continuitatea demersurilor strategice și cu capacitatea de implementare actuală, cu focus explicit pe armonizarea și modernizarea abordărilor și metodelor utilizate, formarea unei resurse umane de calitate și optimizarea comunicării rezultatelor cercetării. Pentru prezenta aplicație ne propunem:

Ob. 1. Întărirea și dezvoltarea colaborărilor naționale și internaționale care să contribuie la i) consolidarea unor domenii de cercetare noi, interdisciplinare, emergente sau a unor domenii prioritare, prezente în top 500 QS, prin transferul de know-how și ii) perfecționarea resursei umane din UB: personal administrativ și didactic ori de cercetare.

Ob. 2. Consolidarea capacității de susținere a cercetării de excelență în UB prin modernizarea și optimizarea utilizării infrastructurilor de cercetare proprii, dezvoltarea instrumentelor de monitorizare scientometrică internă și spijinirea publicării rezultatelor cercetării.

Ob. 3. Promovarea rezultatelor cercetării prin: i) organizarea de evenimente științifice de anvergură și prin ii) dezvoltarea componentei de comunicare și diseminare a științei către publicul larg, având în vedere nevoia de validare pe scară largă a științei ca sursă de informare corectă și ca suport de înregistrare și conservare riguroasă a referințelor, precum și de consolidare a statutului cercetătorului în societate și de cultivare a interesului pentru știință și cercetare.

Consolidarea și creșterea competitivității instituționale a Universtății din București ca hub de proiecte interdisciplinare și instituție-gazdă pentru cercetători de top din țară și străinătate Call name: Fondul de Dezvoltare Instituţională - FDI - Competiţia 2020
CNFIS-FDI-2020-0355 2020 -

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation:

Project website:

Abstract:

Obiectivele și activitățile proiectului CREATOR sunt reprezentative pentru interesele de dezvoltare și de consolidare a unor direcții noi, interdisciplinare sau prioritare de cercetare, atât ale facultăților de științe experimentale și teoretice, cât și ale celor din domeniile socio-umaniste.

Prin obiectivele propuse, proiectul CREATOR va contribui semnificativ la extinderea dimensiunii internaționale a cercetării în UB și la dezvoltarea unor 1) domenii de cercetare noi, interdisciplinare, de mare actualitate (Arheoștiințe / ArchaeoSciences) și 2) domenii bine definite, considerate ca prioritare la nivelul UB (sănătate, fizica energiilor înalte, noi materiale).

Ob. 1. Susținerea perfecționării resursei umane în cadrul unor laboratoare de top din străinătate, în vederea alinierii cercetătorilor din UB la standardele de performanță internaționale actuale în domeniile vizate de acest proiect.

Ob. 2. Întărirea capacității de susținere a cercetării de excelență în UB prin îmbunătățirea utilizării/mentenanța infrastructurii de cercetare existente, integrarea unor cercetători de top din străinătate, care să asigure un transfer de know-how științific și de bune practici în UB și popularizarea cercetării în mass-media.

Ob. 3. Creșterea capacității de administrare a activității de cercetare și optimizarea colectării și stocării rezultatelor cercetării, prin dezvoltarea unei platforme electronice integrate.

FILE DESCRIPTION	DOCUMENT
List of research grants as project coordinator	Download (14.45 kb) 05/04/2017
List of research grants as partner team leader	Download (15.95 kb) 05/04/2017
List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
R&D activities in enterprises
Peer-review activity for international programs/projects