BrainMap

Mrs. Mihaela Gheorghiu

Principal Researcher I, Dr. Habil.

Principal Researcher, Dr. Abil. - CENTRUL INTERNATIONAL DE BIODINAMICA

Researcher | Scientific reviewer

Curriculum Vitae (30/04/2020)

Expertise & keywords

Electro-Plasmonics for the analysis of the dynamics of cellular processes and biomolecular interactions - BioScope Call name: Complex Exploratory Research Projects - PCCE-2011 call
PN-II-ID-PCCE-2011-2-0075 2012 - 2016

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO); SC INSTITUTUL DE OPTOELECTRONICA SA (RO); CENTRUL INTERNATIONAL DE BIODINAMICA (RO); Universitatea din Bucuresti (RO); CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website: http://www.biodyn.ro/php/biodyn.php?act=Projects&idm=5&idsm=11&locale=en

Abstract:

Whereas experiments on processes at single cell level are highly demanded by biomedical industry they currently raise serious technical challenges. Therefore, BioScope project advances the State of the Art by proposing creation of the Electro-Plasmonic Analysis System (EPAS), an innovative engineered system integrating advanced optics and electronics that will reveal the dynamics of electrical and morphological properties of relevant intracellular structures and biointerfaces with an unprecedented temporal (1 ms) and spatial (200 nm) resolution.

A multidisciplinary consortium will develop EPAS by harnessing Surface Plasmon Resonance, SPR, Magnetism (to increase SPR and thus overall sensitivity), Optogenetics (to control cell properties through light for calibration purposes), Modelling (to reveal details of how cells in electric field affect SPR), Bioinformatics (for the analysis of data) and Biochemistry (to validate/substantiate the results).

Novel analytic capabilities of EPAS to perform fast, noninvasively and label free, thorough in situ measurements for gaining new insights into cellular processes (such as changes in adherence, conformation, and organelle distribution, swelling / shrinking, exo- and endocytosis, neurotransmitter release, electrical spiking, etc.) at single cell level will be explored.

BioScope results will facilitate creation of the next generation of (single)cell-based analytical platforms likely to support breakthrough applications spanning from quality control of food and aquatic media to bio-medical ones coping with analysis of interaction mechanisms between selected cells and various stimuli (including those fostering new pharmacological products), cell assays as alternatives to animal (preclinical) testing, or last but not least, supporting cutting-edge cell-based “Disease in a Dish” approaches.

Tracing proteins through food processing with biosensors Call name: Projects for Young Research Teams - TE-2011 call
PN-II-RU-TE-2011-3-0302 2011 - 2014

Role in this project: Key expert

Coordinating institution: Centrul International de Biodinamica

Project partners: Centrul International de Biodinamica (RO)

Affiliation:

Project website: http://www.biodyn.ro/PROJECTS/Contract%20No.%2006-05.10.2012/urmarirea%20proteinelor.pdf

Abstract:

The overall objective of the project is to explore the possibility of tracing proteins through food processing using biosensors, responding to a real societal need originating from current regulatory and food industry requirements defining maximum admissible limits of allergen protein in food. Three main goals around which the activity of a new, young research team will be centered will be put forward in an integrated analytical platform: Develop new biosensors for sensitive and specific detection of allergen proteins; to assess protein interactions in food matrices; demonstrate the feasibility of developed methods/sensors for tracing proteins through food processing by three practical applications relevant for food industry. Comparison of proposed sensors and procedures with current reference methods is envisaged for each of the three practical applications. The project fosters novel biosensors and working protocols related to: a simplified, sensitive detection method for allergen proteins, contributing to public safety and food quality control (a) and a new platform for studying protein interaction, aimed to study the correlation between changes in the conformation of a specific protein and parameters related to food formulation or manufacturing (b). At the end of the project, it is expected that a strong team will be consolidated and will acquire momentum to attract new funding and participate in larger research projects.

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