BrainMap

Mr. Marius VOLMER

Dr.

Associate Professor - UNIVERSITATEA TRANSILVANIA BRASOV

Researcher | Teaching staff | Scientific reviewer

Curriculum Vitae (22/03/2022)

Expertise & keywords

Immunoassay Lab-on-a-chip for cellular apoptosis study Call name: Joint Applied Research Projects - PCCA-2011 call, Type 1
PN-II-PT-PCCA-2011-3.1-0052 2012 - 2016

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE- DEZVOLTARE PENTRU MICROTEHNOLOGIE - IMT BUCURESTI INCD

Project partners: INSTITUTUL NATIONAL DE CERCETARE- DEZVOLTARE PENTRU MICROTEHNOLOGIE - IMT BUCURESTI INCD (RO); UNIVERSITATEA TRANSILVANIA BRASOV (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Affiliation: UNIVERSITATEA TRANSILVANIA BRASOV (RO)

Project website: http://www.imt.ro/cellimmunochip

Abstract:

The ambitious goal of this project is to combine fundamental research with complex applicative research in order to bring important scientific contributions both on the theoretical level and on the experimental one, in a high-end field: bio-nano-engineering, a new field in Romania and in expansion stage worldwide. Bio-nano-engineering assumes the integration of the physico-chemical processes with the biological ones by involving complex systems realized on the micro or nano scale, the applications of these systems being found in biomedicine The aim of this project is the development (design, fabrication and characterisation) of a versatile lab-on-a-chip integrated system, which is composed of a microfluidic platform, an interdigitated micro/nano-electrodes electrochemical biosensor and an array of spin valves for superparamagnetic nanoparticles - based immunoassay, and will be applied to the study of cellular apoptosis and detection of specific antigens as clinical diagnostics application. The screening reaction is based on a competitive immunoassay, enhanced by using functionalized superparamagnetic nanoparticles, prepared through a simple and reliable surface modification and protein conjugation process. The main advantage of this system is its re-usability. Also, its configuration can be changed easily so the user can try several electrode configurations with the same holder. Moreover, because the functionalized nanoparticles are magnetically trapped above the electrodes, the user can adjust the fluid flow rate to the kinetic parameters of chemical reactions under study to optimize detection. The superparamagnetic nanoparticles can be trapped by polarization of the spin valve array and can be released by blocking the magnetic field. We shall demonstrate how to take further advantage of the microfluidic system to determine enzyme activities or concentrations, as flow velocity can be adjusted to the rate of the reactions under study.

FILE DESCRIPTION	DOCUMENT
List of research grants as project coordinator
List of research grants as partner team leader
List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
R&D activities in enterprises
Peer-review activity for international programs/projects