BrainMap

Maria-Cristina Radulescu

Ph.D.

- INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Researcher | Scientific reviewer

Web of Science ResearcherID: https://publons.com/researcher/2808395/cristina-m-radulescu/

Expertise & keywords

Fast analysis method of aspartame from foods using an amperometric enzymatic biosensor Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-0503 2017 - 2018

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://www.incdsb.ro/p/Aspsense/PED-180-ASPSENSE.htm

Abstract:

This demonstrative project aims to develop and validate in laboratory a fast analytical method for detection of aspartame from food samples based on a combination of an amperometric enzymatic biosensor and a Low-noise potentiostat. The growing use of aspartame in the food industry requires the development of rapid and effective methods for its determination. The project aims to provide a cost effective and easy to use analytical method of aspartame. The detection principle of the biosensor is based on enzymatic oxidation of aspartame catalyzed by carboxyl esterase (CaE) and alcohol oxidize (AOX), with the production of hydrogen peroxide that is electrochemically quantified. The developed potentiostat used for the quantification of the biosensors’ amperometric signals has two building blocks: (i) a potentiostat circuit that provides constant continuous voltage and (ii) current sensing circuit to quantify the current solution ICELL related with the concentration of aspartame. Low-pass filter will be added to the current sensing circuit’s feedback loop to diminish the induced electromagnetic noise effects. The consortium combines the vast experience in the field of biosensors and enzymology of National Institute of R&D for Biological Sciences with the know-how in electronic potentiostat design of the University Politehnica of Bucharest.

Sensitive and selective detection of neurotoxic insecticides based on cholinesterase biosensors coupled with solid phase extraction Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-0367 2017 - 2018

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); CP MED LABORATORY SRL (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://www.incdsb.ro/p/COLIN-SP/PED45-2017.htm

Abstract:

This applicative project aims to develop a selective and sensitive analytical method useful for screening of neurotoxic insecticides in environmental samples based on a combination of the specific advantages of acetylcholinesterase biosensors and solid phase extraction (SPE). The biosensors provides the low-cost and easy to use analytical devices while the sample pretreatment with SPE ensures the required sensibility and results reliability due to interference removal from unknown samples. The successful combination of biosensor with SPE is based on optimization of the enzymatic inhibition buffers that contains optimized percentages of various water miscible organic solvents and choice of the appropriate sorbent-elutant for analyte separation. A heterogeneous oxidation of the organophosporothionate insecticides on the SPE column will be tested for improved sensitivity. The consortium combines the vast experience in the field of cholinesterase biosensors and enzymology of National Institute of R&D for Biological Sciences with the know-how of S.C. CP MED Laboratory S.R.L. in solid-phase extraction, reliable analyses for environmental pollution monitoring and method validation.

Multiparametric system for wine monitoring fermentation Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0297 2014 - 2017

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); UNIVERSITATEA BABES BOLYAI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); CEPROCIM S.A. (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://www.incdsb.ro/p/Senswine/PCCA0297-SENSWINE-WEB.htm

Abstract:

The objectives of this project are the development of a complete analytical solution for the wine sector consisting of: (1) a multiparametric flow analysis system based on enzymatic biosensors for monitoring of alcoholic and lactic must fermentations (2) mineralogical and geochemical investigations of soil from vineries and (3) detection of heavy metals from soil and winemaking relevant matrices (grapes, must and wine). The European winemaking sector is facing an intense competition from imports of low-cost wines and is under pressure to improve the products quality. Because the winemaking process is an “art” highly dependent on the producers' experience and skill, fast, cheap and reliable analytical devices and services are vital to obtain an effective control at all stages of the production. The current analysis techniques (FTIR or chromatographic analyses) are long and expensive due to complexity of the sample matrix and too complex to be used in situ by the average winemaker. This multidisciplinary project will develop two enzymatic biosensors for the detection of alcohol (based on oxidases) and respectively lactic acids (based on dehydrogenases). The biosensors may be used independently or integrated in a multiparameter flow analytical system that will quantify the signal using a analog potentiostat. The heavy metal detection is important for two reasons: (1) they are involved in taste and flavor development and (2) to protect the consumers of contaminated products. The soil has a major influence on the grape quality and provides the wine specificity that determine the consumers fidelity for the products obtained from a specific "terroir". The combination of all three analytical systems provides a complete solution adaptable to the specificity of winemaker from various European countries each one with different domestic market and background.

New molecularly imprinted polymers (MIPs) developed for the sensitive and fast analysis of nitrofuran based antibiotics Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0203 2014 - 2017

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); COSFEL ACTUAL S.R.L. (RO); CP MED LABORATORY SRL (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://www.chimie.unibuc.ro/cercetare/analitica/proiect%20197_2014%20Victor%20David.pdf

Abstract:

The objectives of this project are the synthesis of two molecularly imprinted polymers (MIPs) and their use for the development of two analytical methods for the detection of nitrofuran based drugs/metabolites. The illegal use of nitrofuran based drugs (furazolidone, furaltadone, nitrofurantoin, nitrofurazone, etc.) by the livestock industry as growth promoters leads to the apparition of new dangerous bacteria strains that are resistant to antibiotic and produces dangerous metabolites that remain for a long time in meat. The EU regulations impose the control of biological relevant samples collected from living animals (urine, blood), meat from slaughter houses or traders and prepared foods. The current analysis techniques are long and expensive due to complexity of the sample matrix and low concentration of the analytes. The two MIPs synthesized in this project will be used in two different analytical applications: (1) the development of affinity based sensors and (2) for sample pre-treatment by solid phase extraction (SPE) before chromatographic analysis. The sensors will be used for rapid screening of biological samples and constructed by the immobilization of the MIPs on a transducer and detection using an appropriate detection technique like electrochemical impedance spectroscopy (EIS) or quartz crystal microbalance (QCM). The main advantages of the sample pre-treatment with MIPs in chromatographic analysis are analyte isolation and concentration from complex matrices. Due to the high polarity and low detectability in UV-VIs absorption, the project will be focused also on derivatization of chosen analytes in order to enhance their hydrophobicity and fluorescence labeling. This procedure is carried out after extraction of analytes from samples by means of MIPs. The project is industrial driven and the consortium will take important steps in order to prepare a successful commercialization of the developed MIPs such as: (1) the MIPs development and optimization at laboratory scale will take into account not only the analytical performances, but also the requirements to adapt the synthesis to the industrial mass production, (2) the production of the developed MIPs will be tested at industrial scale level by the SME that will continue the post-project production and (3) the developed MIPs will be thoroughly tested by a laboratory specialized in analytical services in order to evaluate the fulfillment of end-user needs.

Versatile microelectrodes surface modification method for enzymatic microbiosensor development with applications for cell cultures monitoring Call name: Projects for Young Research Teams - TE-2011 call
PN-II-RU-TE-2011-3-0076 2011 - 2014

Role in this project: Key expert

Coordinating institution: Institutul National de Cercetare Dezvoltare pentru Stiinte Biologice Bucuresti

Project partners: Institutul National de Cercetare Dezvoltare pentru Stiinte Biologice Bucuresti (RO)

Affiliation: Institutul National de Cercetare Dezvoltare pentru Stiinte Biologice Bucuresti (RO)

Project website: http://www.bioanaliza.ro/microsites/te76-WEB.htm

Abstract:

The use of microelectrodes and microbiosensors in analytical chemistry has several advantages like a better limit of detection or possibility to obtain information on localized phenomena, but their application is restricted by the difficulties in specific surface modification with electrodes and enzymes immobilization. This project aims to develop a versatile method for enzymatic microbiosensors development. An electrochemical mediator will be electrodeposited on the surface of the microelectrode and subsequently a FAD dependent enzyme will be immobilized. During the developed of microelectrode modification method there will be chosen strategies that are easily adaptable to other electrode substrates and mediators (e.g. diazonium bonds may be made with different materials while thiols may bind only on gold surfaces). The modification methods are directed on microelectrode surface (e.g. electropolymerization takes place only on electrode surface while a co-precipitation leads to product deposition also on microelectrode support). In the end, the microbiosensor will be used for monitoring of cell cultures. Other possible applications of the microelectrode modification methods are in other areas of analytical chemistry like EAFM or SECM or the improvement of fuel-cells.

Development and characterization of molecular sensors based on derivates of ciclobis(paraquat-p-phenilen) used for detection and identification of local factors generating crises (dangerous and priority dangerous substances) Call name:
PN-II-2008-2011 2008 - 2011

Role in this project: Partner team leader

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website:

Abstract:

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List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
R&D activities in enterprises
Peer-review activity for international programs/projects