BrainMap

Mrs. Lia-Mara Ditu

Associate professor

- UNIVERSITATEA BUCURESTI

Researcher | Teaching staff | Scientific reviewer

I am Associate Professor and the Head of Botanic and Microbiology Department, Faculty of Biology, University of Bucharest, Romania. My teaching activity include Applied Microbiology, Epidemiology, Clinical Virology, Applied Mycology, Industrial Microbiology, coordinating at least 5 bachelor theses,10 dissertation theses per year and over than 10 PhD students from Doctoral School of Faculty of Biology and Doctoral School of Faculty of Chemical Engineering and Biotechnology, National University of Science and Technology Politehnica Bucharest. According to Web of Science Core Collection, I published 12 international book chapters, 100 ISI papers. My publications gathered 1342 citations and Hirsh = 19 (Web of Science Core Collection). I participated at international conferences with over 60 posters/ oral presentations, I registered 2 national patents. Also, I am reviewer for 10 ISI journals and member of the editorial board for Biomedicines.

Curriculum Vitae (12/09/2024)

Expertise & keywords

Through nanotechnology towards the next generation dental restorative materials Call name: P 4 - Proiecte de cercetare exploratorie - PCE-2021
PN-III-P4-PCE-2021-0292 2022 - 2024

Role in this project:

Coordinating institution: Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti

Project partners: Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO)

Affiliation:

Project website: https://icechim.ro/project/nanodent_en/

Abstract:

The acidic attack from cariogenic bacteria found in dental plaque biofilm growth over the tooth is responsible for dental caries, also known as tooth decay, which is a biofilm-dependent infectious disease that damages teeth by minerals loss and presents a high incidence of clinical restorative polymeric fillings. The complexity of oral biofilms contributes to the difficulty in developing effective novel dental materials. Oral cavity represents an ideal environment for the microbial cell growth, persistence, and dental plaque establishment. When the complexity and volume of biofilms from the gingival crevice increase, chronic pathological conditions such as gingivitis and periodontitis can occur, predisposing to a wide range of complications. In this context, nanotechnology has potential to be explored in the development of bioactive dental materials to reduce or modulate the activities of caries-related bacteria. The project NanoDent proposes an original recipe based on nanometric phosphatic compounds enriched with phytosynthesized metallic nanoparticles and natural bioactive compounds, with simultaneous antibacterial and enhanced mechanical properties as a potential dental restorative material. The validation of the proposed technologies to obtain novel materials will be obtained via third-parties studies, performed through subcontracting.

Innovative multifunctional composites for the protection of cultural heritage objects Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-0627 2022 - 2024

Role in this project:

Coordinating institution: Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti

Project partners: Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO); UNIVERSITATEA NAŢIONALĂ DE ŞTIINŢĂ ŞI TEHNOLOGIE POLITEHNICA BUCUREŞTI (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation:

Project website: https://icechim.ro/project/inheritage_ro/

Abstract:

In the last decades, new perspectives have emerged among both specialists, and the general public, regarding the preservation and enhancement of the cultural heritage. Conservation-restoration contribute to the safeguarding and understanding of the cultural property that benefits present and future generations, in its historical and aesthetic sense, in its physical integrity, its context and its uses. In the last years, the specialists in the field of materials science proposed a series of innovative materials and methodologies, applied in different areas, including the diagnosis and conservation state monitoring or preventive measures, implemented in order to reduce the need for interventions. However, as the degradation processes of cultural heritage objects remain inevitable, the continuous development of advanced materials able to counteract specific degradation processes is also necessary. Thus, are needed thorough scientific studies in order to develop tailored formulation, in order to protect, preserve, and restore cultural heritage objects, and this represents a continuous challenge for the scientists, aiming to replace the current rather serendipitous approaches in restoration. In this context, the project “Innovative multifunctional composites for the protection of cultural heritage objects” (INHERITAGE) aims to develop novel multi-layer composite materials with multiple functions (consolidation, self-cleaning and anti-microbial) applicable for the conservation of different inorganic substrates.

TRANSDISCIPLINARY APPROACH FOR DEVELOPMENT OF TECHNOLOGICAL SOLUTIONS FOR TARGET COMPOUNDS RECOVERY FROM AGRO SIDE-STREAMS Call name: P 3 - SP 3.5 - Proiecte EUREKA Tradiţional (Network), EUREKA-Cluster, Eurostars
PN-III-P3-3.5-EUK-2019-0226 2020 - 2023

Role in this project:

Coordinating institution: HOFIGAL EXPORT IMPORT SA

Project partners: HOFIGAL EXPORT IMPORT SA (RO); Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO); UNIVERSITATEA DE STIINTE AGRONOMICE SI MEDICINA VETERINARA (RO)

Affiliation:

Project website: https://hofigal.eu/content/26-proiect-tardis-hofigal

Abstract:

The project TARDIS is proposed in the context of closing chains in bioeconomy, in order to obtain extracts with increased target bioactive content, with potential pharmaceutical applications (Pharmacoidea) and/or nutraceutical products (HOFIGAL). Biowaste valorisation is an attractive approach in the framework of the EU Waste Management policies and the development of a circular economy. Wastes from biostreams and different bio-based sources are being under-utilized as potential resource of valuable compounds.

The general objective of the project is to underline, optimize and apply an integrate technology of sustainable and enhanced production of natural extracts with high content of biological active compounds from side streams of agricultural industries applicable for obtaining smart formulations for disorders of the 21st century, for which continuous research is carried out.

The main idea of the project is to process plants by-products rich in nutritional and bioactive compounds into formulations to be used in pharma industry with the following benefits: the use of obtained extracts and target compounds for the prevention / treatment of age-related neurodegenerative disorders such as Alzheimer's disease; minimizing the amount of plant waste by recovering the target compounds from the by-products; increasing the potential of the pharmaceutical and food market by designing new functional ingredients; increasing the quality of life.

The project will provide the necessary support for creating and developing a complementary research chain in bio-economy (industry, pharmacy, agriculture) that aims to achieve a high level of internationally recognized expertise. The project demonstrates an integrated cascading concept including pre-treatment, optimized extraction and separation technologies.

Novel strategies to improve the performances of medical textiles Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-4926 2020 - 2022

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://lspi.inflpr.ro/2020/PED555/IMEDTEX

Abstract:

We propose a new generation of chronic wound dressings with enhanced healing rates and protection of the lesion from outside pathogens. Medical grade cloth will be surface engineered on the side in contact with air, to become superhydrophobic and antimicrobial via thin film coating with black ZnO (nonstoichiometric, vacancy, defect containing ZnO), synthesized by Pulsed Laser Deposition.We showed in a previous research that black ZnO is both superhydrophobic and antibacterial/antifungal. The inner side of dressings, in contact with the wound, will be covered by Matrix Assisted Pulsed Laser Evaporation with a hydrophilic mixture consisting of antibiotics, epithelial growth factors and analgesic. Linezolid/dicloxacillin/neomycin will be used as antibiotics with efficiency against both Gram negative and Gram positive bacteria, hyaluronic acid as growth factor in tissue reconstruction and a neuropathic agent with analgesic and anti-inflammatory effects. The novelty of the Project comes from the use of laser deposition techniques which can be tuned to synthesize both organic and inorganic adherent coatings with precise control of thickness and minimal consumption of precursors.

The correlation between coating’s properties of interest and their morphology or composition will be made after thorough tests by atomic force and scanning electron microscopy, profilometry, wettability assessment, Xray diffraction and Fourier transform infrared spectroscopy.

ZnO films antimicrobial properties will be studied against Gram positive and Gram negative bacteria (bactericide effect, antibiofilm assessment). The biocompatibility and the efficiency of healing factors in form of thin films will be studied in vitro on epithelial lines of cells.

Nano-modified surfaces for G-tubes resistant to microbial colonization Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-3829 2020 - 2022

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://nanopeg.inflpr.ro/

Abstract:

Despite their great benefits for debilitated patients, gastrostomy tubes (G-tubes) are prone to become easily colonized by resident and opportunistic microorganisms, which have the ability to attach on their surfaces and form highly specialized communities called biofilms, which are extremely resistant to host defense mechanisms and antibiotic treatment, leading to treatment failure and device replacement. The scope of this project is to develop an improved G-tube with nano-modified, non-citotoxic and uniform surface, containing core/shell magnetite@silica nanoparticles functionalized with the plant derived compound eugenol (Fe3O4@SiO2@E), to limit microbial colonization and biofilm formation on such devices. The product will consist in a nano-modified surface (obtained by matrix assisted pulsed laser evaporation, MAPLE), biocompatible, resistant to microbial colonization and biofilm formation, to obtain improved G-tubes. We will start with the synthesis and characterization of the nanosystem, followed by the design of nano-modified G-tube surfaces, their quality assessment through physico-chemical, microbiological tests and cytotoxicity assays. By the proposed approach, our project falls within the TRL2-TRL3 level; since after observing the basic principles, we developed an application (antimicrobial and antibiofilm nanosystem) (TRL2), which can now be translated into an improved device (G-tubes with nano-modified surfaces by using nanosystems functionalized with E) able to avoid microbial colonization and biofilm development (TRL3). By its innovative and multidisciplinary approach, this project brings important advances on the field of improved indwelling devices, which, in the current context of the antibiotic crisis and biofilm-related infections represent a top priority at national and international level.

Advanced material based on push-pull extended π-conjugated azo-chromophores in functional matrices with enhanced NLO properties Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-3009 2020 - 2022

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte-de-cercetare/proiecte-cu-finantare-nationala/smart-nlo/?lang=en

Abstract:

Scope of the project SMART-NLO is to obtain, optimize and validate the technology of fabrication and the new product based on novel extended azo chromophores in organo-modified silica films, possesing superior NLO properties at TRL4, developed starting from the preliminary results related to other types of chromophores with nonlinear optical (NLO) properties, filmogenic modified silica films and nanocomposite clay-silica materials obtained at TRL2 already achieved by the team’s publications and the existing laboratory techniques. The method of fabrication of novel material consists in encapsulation of the novel chromophores with extended structures in a silica film rational designed to ensure enhanced nonlinear optical properties. Methods of preparation and composition of the silica filmogenic matrix will be adjust to render tunable and efficient advanced materials with suitable arrangement of chromophore molecules and improved stability, for the fabrication of hybrid materials with superior NLO properties. The methods of film formation are chemicals ones, selected to be energy saving and eco-friendly.

Novel photocatalytic activated resin formula for microbial colonisation control Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-1825 2020 - 2022

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); CHIMTITAN SRL (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte-de-cercetare/proiecte-cu-finantare-nationala/photores/ https://unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte-de-cercetare/proiecte-cu-finantare-nationala/phot

Abstract:

Preventing and controlling the spread of healthcare associated infections is the greatest challenge of a health system. Control authorities have reported, over the past few years, the increasing morbidity and mortality rates of these infections, which is causing a growing pressure on public health systems. In this context, the project aims to design, develop and validate a novel photocatalytic activated product based on resins, in order to prevent and control the microbial pathogens implicated in healthcare associate infections. This product is recommended for hospitals, public institutions or HoReCa companies that want to prevent and combat the spread of pathogenic microorganisms. In recent years, both the medical field and the HoReCa have reported the spread of pathogens that endanger public health. It is also desired to find an economically efficient method to reduce the high costs, and from a microbiological point of view, which kills and inhibits the growth of microorganisms, due to the fact that bacterial and fungal resistance to antibiotics has been reported. Also in the field of HoReCa it is desired to decrease the chlorine based products (sodium hypochlorite) used in disinfection, which can have adverse effects or prove to be inefficient because of the resistance mechanisms developed by different microbial strains. The project proposes the following specific objectives: (1) Obtaining and selecting photoactivated resin formulations, based on physical and chemical properties and testing them for antimicrobial and antibiofilm properties; (2) Demonstrating of the functionality and stability of the selected photoactivated resins in contact with various surfaces (concrete, wood, plastic, stone) and the retesting of the antimicrobial and antibiofim properties; (3) Project management and dissemination of the project results; (4) Meetings with the industrial partner in order to establishe a a good flow of work stages and research results.

In vitro interactions of nanoparticles with human microbiota and probiotic strains Call name: P 1 - SP 1.1 - Proiecte de cercetare pentru stimularea tinerelor echipe independente
PN-III-P1-1.1-TE-2019-1506 2020 - 2022

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://alina.amgtranscend.org/?page_id=89

Abstract:

A large amount of recent scientific evidence supports the idea that microbiota-based therapies can be used to modulate host metabolism and the development of numerous diseases, such as metabolic syndrome, obesity, inflammatory bowel disease, liver diseases, emerging infectious diseases, diabetus mellitus, atherosclerosis, cancer, allergies, and central nervous system disorders, including depression, anxiety, Parkinson’s disease, Alzheimer’s disease, multiple sclerosis and symptomologies of autism spectrum disorder. Such approaches are expected to move from bench to bedside in the near future. From ingested food, to the use of daily products that can be easily ingested, and also to the use of nanosized drug delivery systems for therapy, resident microbiota is prone to be “influenced” by these nanomaterials in widely undiscovered ways. The aim of this project is to investigate the in vitro interactions among inorganic nanoparticles (NPs) widely encountered in common practices and cultivable microbiota and probiotic isolates. Establishing the impact of such nanomaterials, in terms of antimicrobial properties, growth inhibition and antipathogenic protection properties (i.e. modulation of the microbial attachment, pathogen growth inhibition, antibiotic resistance control etc) on model microbiota with probiotic potential species would significantly improve the current knowledge regarding microbiota modulation. Since the microbiota has the ability to control the progress of numerous modern diseases, the impact of such nanosized materials in their development and clinical outcome via microbiota modulation (especially infection control) could be significant.

Improving the management of bedsores by using efficient wound dressings with plant extracts Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-5236 2020 - 2022

Role in this project:

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); HOFIGAL EXPORT IMPORT SA (RO)

Affiliation: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Project website: http://www.smmmf.pub.ro/research/projects/biocure

Abstract:

The project addresses a topic which is very modern and actual at national and international level in the field of biomaterials used as drug delivery systems, namely the bedsores type wound management. These wounds have a high and growing importance in the recent years, being one of the mortality main causes associated with immobility in case of paralyzed, comatose or restrained patients, very high costs being involved for treating bedsore complications and consequences. For this purpose, the project proposes the development of intelligent biopatches as concrete and convenient solution for the topical treatment of bedsores, avoiding in this way the affected area debridement or surgical interventions on larger areas. The BIOCURE wound dressing, object of this research, consists of a protective liner, baking layer, adhesive system and drug and essential oils encapsulated release support, the drug and essential oils being released in a controlled manner directly at cutaneous wound level in order to maintain all time a constant concentration on wound. As biodegradable polymers for loading antibiotic drugs and microcapsules of essential oils we will use some compositions of collagen, poloxamer, alginate, to offer an adequate support for drug release. The essential oils will be encapsulated within dendritic polymers like β-cyclodextrins. In the frame of this project, we will develop a classical acrylic adhesives and a new advanced adhesive from the categories of soft silicone adhesives which will be used in the development of the substrate-adhesive component of the biopatches. The biopatches prototypes obtained within this project will include both the component biopolymer support for and essential oils encapsulated, having optimal release characteristics in relation with the application site and therapeutical indication, and also the substrate-adhesive component biocompatible and adequate for application in cutaneous wounds like bedsores.

Comprehensive Approach to support Precision Agriculture and environmental management through satellite technologies and classic methods of investigation Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-3495 2020 - 2022

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU OPTOELECTRONICA INOE 2000 INCD

Project partners: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU OPTOELECTRONICA INOE 2000 INCD (RO); UNIVERSITATEA DE STIINTE AGRONOMICE SI MEDICINA VETERINARA (RO); Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRONOMICE SI MEDICINA VETERINARA (RO)

Project website: http://environment.inoe.ro/article/253/about-capa

Abstract:

The project ‘Comprehensive Approach to support Precision Agriculture and environmental management through satellite technologies and classic methods of investigation – CAPA’, addresses a major direction of the 21st century, namely, smart agriculture, bringing new insights into how the climate affects the phenological cycle of crops (through the occurrence of extreme events) and it also contributes to understanding the role of different air pollutants (such as greenhouse gases) in crop development. The project proposes the realization of synergies of the scientific activities integrating data on the complex eco-agro-system-culture relations, obtained by applying a conglomerate of methods and techniques (space technologies, the study of pollutants at the level of air, water, soil and plants), for a modern management of agricultural crops, in the context of intelligent agriculture. At the international level, smart agriculture is also based on the optimized management of the inputs according to the real needs of the crops, for their management and the reduction of the use of fertilizers, fungicides, pesticides and water resources. At the national level, the exploitation of satellite records in the field of agricultural management has not yet a significant spread, being used mainly in applications dedicated to environmental protection, forestry, territorial planning or in the military field. In this context, the project proposes the application and integration of spatial technologies together with in situ and ex situ analyses in the intelligent management of agriculture to monitor certain types of agricultural crops (from a pilot site), thus allowing the development of a successful model for better capitalization. of available resources.

COLD PLASMA FOR FLUORIDE RETENTION IMPROVEMENT AND BIOFILM MODULATION IN DENTAL APPLICATION Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-4569 2020 - 2022

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE "CAROL DAVILA" (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://alina.amgtranscend.org/?page_id=87

Abstract:

Despite the technological progress of the last decade, dental caries is still the most frequent oral health threat in children and adults alike. Such condition has multiple triggers and is caused mainly by enamel degradation under acidic attack of microbial cells, which compose the biofilm of the dental plaque. The biofilm of the dental plaque is a multispecific microbial consortium, which periodically develops on mammalian teeth, and it can be partially removed through mechanical forces by individual brushing or in specialized oral care medical facilities. Inhibition of microbial attachment and biofilm formation, as well as methods to strengthen dental enamel to microbial attack represent the key factors in caries prevention. The purpose of this project is to elaborate a cold plasma based method in order to modulate microbial attachment and biofilm formation and to improve the retention of fluoride in an enamel-like model. In our approach, the obtained cold plasma will be tested for its capacity to determine fluoridation and improve fluoride retention in a hydroxyapatite based enamel-like material. Moreover, the impact of such cold plasma on the development of microbial biofilms under various relevant conditions will be established by in vitro studies. In this project, we will start with a technology-formulated concept based on a cold plasma treatment (TRL 1-2), and we will end by validating the technology in laboratory conditions, enlightening the plasma ability to improve fluoride retention and modulate microbial biofilm formation in an enamel-like hydroxyapatite based model (TRL 4). The obtained cold plasma with dual effect exhibited through biofilm modulation and enamel strengthen through fluoridation is intended for dental application such as the prevention and therapy of dental caries and enamel deterioration.

Selection and dissemination of antibiotic resistance genes from wastewater treatment plants into the aquatic environment and clinical reservoirs Call name: P 4 - Proiecte Complexe de Cercetare de Frontieră
PN-III-P4-ID-PCCF-2016-0114 2018 - 2022

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE -DEZVOLTARE PENTRU ECOLOGIE INDUSTRIALA - ECOIND (RO); INSTITUTUL NATIONAL DE BOLI INFECTIOASE ''PROF.DR.MATEI BALS'' (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE MEDICO-MILITARA „CANTACUZINO” (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://bios.unibuc.ro/Proiecte/pn-iii-p4-id-pccf-2016-0114.html

Abstract:

The RADAR project aims to assess the prevalence and dissemination of antibiotic resistance (AR) from urban, clinical and industrial wastewater into the aquatic environment via wastewater treatment plants (WWTPs). Moreover, RADAR will give information on the environmental and clinical resistome, identifying the possible mechanisms of AR emergence and spread.

Scientific objectives: i) investigation of WTTPs resistome to establish the prevalence of AR bacteria (ARB)/genes (ARGs) in the environmental samples from upstream-WWTP-downstream transects; ii) evaluation of selected antibiotics (beta-lactams, fluoroquinolones and macrolides) occurrence in both WWTP’ influent and effluent; iii) comparative analysis of geographically and time related ARB/ARGs from wastewaters and clinical sources; iv) assessing and prediction how the presence/absence of ARGs and their relative abundance depending on a class of geographical, hydrological, physico-chemical and microbiological factors.

Methodology: The urban, clinical, farming and industrial wastewater will be monitored by physico-chemical, microbiological methods and metagenomics throughout various steps, i.e.: 1000 m upstream river, influent, different treatment steps inside WWTP, effluent and 200 m downstream river. Wild fish from the receiving river after discharge of WWTP effluent will be also analyzed. The following analyses will be performed: i) LC/MS and HPLC techniques to monitor the levels of selected antibiotics; ii) isolation and identification of ARB belonging to ESKAPE species; iii) evaluation of metagenomic resistome by bioinformatic data processing; iv) environmental and clinical ARGs and plasmids sequencing; v) analyzing the potential of ARGs for transferability and environmental/clinical risk by mapping the insertion loci of transposable elements and the ARGs potential to mobilize the resistance genes in a bioreactor model; vi) elaboration of a prediction model for the occurrence of ARB/ARGs

Antimicrobial Resistance Manure Intervention Strategies Call name: P 3 - SP 3.2 - Proiecte ERA.NET - COFUND
COFUND-JPI-EC-AMR-ARMIS 2018 - 2021

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://bios.unibuc.ro/Proiecte/cofund-jpi-ec-amr-armis.html

Abstract:

Manure is one of the major sources of antimicrobial resistance (AMR) in the environment, since

livestock animals consume the majority of antibiotics produced globally. Antibiotics together

with antibiotic resistant bacteria are excreted to the environment via manure, and may

significantly contribute to the transmission of and exposure to AMR in food, water, and air as

exemplified for methicillin-resistant Staphylococcus aureus (MRSA). Techniques for nutrient

reduction in manure, such as composting and anaerobic digestion, exist and are started to be

applied in a number of countries. These techniques can also reduce antibiotic resistance.

However, to date, no studies simultaneously studied the reduction of all AMR components

(antibiotics, bacteria and genes) by different manure interventions.

In this project, we will measure the effectiveness of different manure treatment techniques on

AMR reduction throughout the manure chain and analyse process parameters of influence.

Both large-scale (centralised) treatment systems and small-scale (farm) systems will be

evaluated in different national contexts with varying AMR prevalence. With culture dependent

and culture independent methods, the abundance of antibiotic resistance and its mobility at

different steps of the manure treatment processes is evaluated. We will focus on ESBLproducing

Enterobacteriaceae, vancomycin-resistant Enterococci (VRE) and MRSA, as well

as metagenomic and qPCR analyses of antibiotic resistance genes, and quantification of

antibiotic residues. Emissions of AMR from manure and manure treatment systems will be

assessed, in order to determine risks of exposure. With input from workshops on risk

perception with relevant stakeholders, these risk assessment outcomes will be communicated

to improve awareness on antibiotic usage, to guide prioritising intervention initiatives, and to

further comprehend exposure risks. Knowledge on the effectiveness of manure interventions

on AMR transmission may contribute to reducing the AMR

impact caused by the livestock industry.

Advanced biodegradable materials based on MgB2 resistant to microbial colonization Call name: P 3 - SP 3.2 - Proiecte ERA.NET - COFUND
COFUND-M-ERA.NET II-BIOMB 2017 - 2021

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA

Project partners: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://infim.ro/project/biomb/

Abstract:

The innovation of this project consists in the evaluation for the first time of the MgB2 potential for biomedical applications, although it is currently produced for superconductivity devices. Expectations are to generate new MgB2-based composite multifunctional biomaterials with antimicrobial/antifouling properties, and an increased biocompatibility at interfaces between the material and the biological media.

The MgB2 powders, coatings and bulks could be used in biodegradable implants or drug delivery systems, handles and surgical tools, catheters, wound dressings and so on. The mechanical and physico-chemical properties of the proposed materials will be investigated by a comprehensive approach, and bioevaluation will include in vitro and in vivo assays. The MgB2 materials are viewed as solutions for space and time- scale controlled variation of the functional properties required for different bio-applications.

Multidisciplinary platform for integrative and systematic research of tangible and non-tangible cultural heritage and identities in Romania Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0686 2018 - 2021

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL DE ARHEOLOGIE "VASILE PIRVAN" (RO); INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA SI INGINERIE NUCLEARA " HORIA HULUBEI " - IFIN - HH (RO); UNIVERSITATEA "ALEXANDRU IOAN CUZA" IASI (RO); UNIVERSITATEA DE VEST TIMISOARA (RO); INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA (RO); INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE IN INFORMATICA - ICI BUCURESTI (RO); UNIVERSITATEA OVIDIUS (RO); UNIVERSITATEA DIN ORADEA (RO)

Affiliation:

Project website: http://patcultro.unibuc.ro

Abstract:

The present project represents an original approach, from an inter-, pluri-, multi- and transdisciplinary perspective of the 6th domains - Patrimony and cultural identity. Through the structure of the consortium, the management of the project, the objectives proposed by the component projects, through the synergies and complementarities obtained, undoubtedly presents a high degree of feasibility, being a sophisticated, integrative and exhaustive approach for Romania. Its results will have an impact both on the national and international scientific communities, but also on the broad public, contributing through the proposed activities to the consolidation, development, and improvement of the RDI system in our country, and indirectly to the training of the future specialists from the Romanian research.

The final goal of the project is to create a complex interdisciplinary/multidisciplinary platform for integrated and systematic research of tangible and non-tangible cultural identities and heritage in Romania.

The consortium that will implement this complex project is coordinated by the University of Bucharest (CO-UB), in partnership with the "Vasile Parvan" Institute of Archeology of the Romanian Academy (P1-IAB), the National Institute for Research and Development for Physics and Nuclear Engineering " Horia Hulubei "(P2-IFIN-HH)," Al. I. Cuza "University of Iasi (P3-UAIC), West University of Timisoara (P4-UVT), National Institute of Research and Development for Material Physics (P5-INCDFM), the "Ovidius" University of Constanta (P6-UOC) and the University of Oradea (P7-UO). The way of geographic distribution at the level of Romania ensures a good regional representation of the legal entities represented within the consortium in this project. The combination of five universities and three national research institutes is the ideal combination to support and implement such a complex project.

Antibiotic Resistance in Wastewater:Transmission Risks for Employees and Residents around Waste Water Treatment Plants Call name: P 3 - SP 3.2 - Proiecte ERA.NET
ERANET-JPI-EC-AMR -AWARE-WWTP 2017 - 2020

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://bios.unibuc.ro/Proiecte/eranet-jpi-ec-amr-aware-wwtp.html

Abstract:

The rise of antibiotic resistant infections is an imminent global public health threat, and mitigation measures are required to minimize the risks of transmission and human exposure. Municipal wastewater treatment plants (WWTPs) are known hotspots for the dissemination of clinically relevant resistant bacteria of human origin to the environment, and simultaneously represent targets for intervention and mitigation strategies. While aerosolized bacteria are found within WWTP, it is largely unknown whether WWTP workers are at risk of elevated resistance carriage. In order to study the occupational and environmental transmission of antibiotic resistance due to human exposure to WWTP-borne bacteria, we will assess carriage of extended-spectrum beta-lactamase (ESBL) and carbapenemase-producing Enterobacteriaceae and resistance genes in WWTP workers, in residents in the proximity of treatment plants, and in water and air samples – both in countries with low and high antimicrobial resistance (AMR). Based on microbial cultivation as well as on high-throughput sequencing data and quantitative real-time polymerase chain reaction (qPCR), exposure through ingestion and inhalation will be modelled, and airborne exposure will be derived from geospatial analyses. Further, we will analyse treatment efficiencies of different WWTP processes in terms of AMR reduction, and therewith identify science-based critical control points for interventions. The focus of this transnational collaboration combining complementary and synergistic European research strengths, is to tackle the increasingly relevant public health threats from antibiotic resistance in WWTP by identifying transmission routes, means of exposure, and proposing risk reduction measures.

Microbial communities associated with romanian rare plants from a natural habitat Call name:
13054/2017 2017 - 2018

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://icub.unibuc.ro/index.php/application/young-researchers-grant-ub/2-uncategorised/75-young-researchers-grant-ub-awarded-in-2017

Abstract:

Plants do not grow axenically in nature, but host a diverse community of microorganisms, termed as plant microbiota, colonizing different niches, both inside and outside their tissues, in the endosphere and ectosphere, respectively. The phylogenetic composition of these communities is composed by relatively few bacterial phyla, including Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria [1]. These microorganisms are involved in major physiological processes, such as plant nutrition and plant resistance to biotic and abiotic stress factors [2].

Our focus is oriented to several rare plant species from natural habitats of Romania which were naturalized and cultivated in the Botanical Garden of University of Bucharest: Adonis vernalis, Opopanax chironium, Paeonia tenuifolia, Potentilla emilii-popii, Asphodeline lutea. The selected plant species are listed with different degrees of danger in the "Red Book vascular plants in Romania" [3] [Dihoru & Negrean 2009], Red Lists IUCN, Bern Convention Habitats Directive, CITES. For Asphodeline lutea (L.) Rchb., the northern boundary of its spreading area in Europe is Northern Dobrogea. Plant growth–promoting microorganisms are mainly soil- and rhizosphere-derived organisms that are able to colonize significantly the plant roots and exhibit a positive effect on the plant growth under certain environmental and soil conditions [6]. A wide range of microorganisms found in the rhizhosphere are able to produce substances that regulate plant growth and development. Bacterial and fungal production of phytohormones such as auxins and cytokinins can affect cell proliferation in the shoot leading to tumoral growth as in the case of Agrobacterium tumefaciens or Ustilago maydis infection, or modify root system architecture by overproduction of lateral roots and root hairs with a subsequent increase of nutrient and water uptake [7]. The purpose of the research is to investigate the normal microbiota of rare plant species (listed with different degrees of danger in the "Red Book vascular plants in Romania", Red Lists, IUCN, Bern Convention Habitats Directive, CITES), found in natural habitats, in order to define some microbiological associate with soil composition that could be further used as parameters for measuring the plant growth.

Composite hydrogels based on inorganic nanoparticles and collagen with prolonged antimicrobial activity for the prevention of wound infections Call name: P 2 - SP 2.1 - Proiect de transfer la operatorul economic
PN-III-P2-2.1-PTE-2016-0177 2016 - 2018

Role in this project:

Coordinating institution: SANIMED INTERNATIONAL IMPEX S.R.L.

Project partners: SANIMED INTERNATIONAL IMPEX S.R.L. (RO); UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://nanocolagel.sanimed.ro/

Abstract:

Chronic wounds represent a good niche for biofilm development because the impaired immune response promotes infection susceptibility whilst necrotic tissue and debris favor bacterial attachment. Collagen hydrogels represent one of the most efficient treatments in case of both chronic and acute wounds due to their ability to maintain optimal humidity and aeration parameters. Currently, at a national level there is no production of collagen hydrogels aimed for the treatment of chronic wounds, hence these types of products are being imported. In this context, the present project proposal aims to design and obtain new types of multifunctional collagen hydrogels harboring antimicrobial properties in order to favor the healing process of chronic wounds. As a novelty element in comparison to products currently available on the international market, we will design and produce collagen hydrogels containing nanoparticles. Thus, Sanimed International Impex S.R.L will develop a simple and rapid technology to obtain hydrogels functionalized with metalic and oxidic nanoparticles (collagen hydrogels with Ag nanoparticles, collagen hydrogels with ZnO hydrogels and collagen hydrogels with SiO2@ZnO nanoparticles). The novel hydrogels will be tested for their antimicrobial and antibiofilm properties using in vitro and in vivo methods and also for the host response after hydrogel treatment on wounded cells and in vivo lesion murine models, for achieving market preparation.

Knowledge Transfer on investigation of the anti-infective and antitumoral activity of novel cosmetic and pharmaceutical formulations based on natural extracts Call name: P 2 - SP 2.1 - Transfer de cunoaștere la agentul economic „Bridge Grant”
PN-III-P2-2.1-BG-2016-0369 2016 - 2018

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO); HOFIGAL EXPORT IMPORT SA (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://icubresearch.wordpress.com/2018/10/19/bg-369/

Abstract:

The present project proposal aims the development of an innovative methodology for testing the antimicrobial, antiviral, anti-tumor and immunomodulatory activity, and to elucidate the mechanisms of action at the cellular and molecular level of natural bee and plant extracts with the purpose of introducing them în new cosmetic and pharmaceutical formulations, for expanding the marketed products range of the economic agent participating în the project - Hofigal S.A.

The addressed strategy is based on abundant scientific literature and original experimental results of all involved partners regarding the characterization and marketing of natural products / compounds with therapeutic or prophylactic value.

The proposed project falls into a major research direction aimed at the discovery of complementary therapeutic solutions based on natural compounds, for the treatment of different pathologies (i.e., infectious diseases and cancer), which are currently found în the top of causes of global morbidity and mortality.

The therapeutic alternatives that will be investigated în this project are based on natural compounds of vegetal and bee origin, with high therapeutic and preventive potential, due to the following properties: complex composition that allows simultaneous action on multiple biological targets; decreased risk for selecting resistance, both în case of infectious agents, such as viral, bacterial and fungal, and în the case of tumor cells; immunomodulatory effect; high biocompatibility; minimal or no side effects.

Bioceramic composites with local applications in antibacterial therapy Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0005 2014 - 2017

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA

Project partners: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); SPITALUL CLINIC DE URGENTA (RO); UNIVERSITATEA BUCURESTI (RO); BIOSINTEX SRL (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.infim.ro/ro/projects/compozite-bioceramice-cu-aplicatii-locale-terapia-antibacteriana

Abstract:

In recent years, much attention has been paid to the synthesis and characterization of nanomaterials because of their interesting properties, which mainly come from the high surface/volume ratio. Hydroxyapatite incorporated with antimicrobial agents is able to prevent or cure infections by releasing directly the antimicrobial agents to local regions. HAp incorporated with Ag/Ag+, Cu2+/Zn2+, ampicillin and doxycycline have been studied to evaluate their antibacterial effect. Among those, Ag incorporation has gained more attention due to its broad-spectrum antibacterial properties. On the other hand in the latest years, the development and the use of antimicrobian agents in treating acute or chronic cutaneous lesions reached a development from the stage of clinical research to the appearance of some commercial products series.

Our project is based on a multidisciplinary approach involving physics, chemistry, microbiology, human toxicology and medicine. The project objectives are to highlight the major contributions that could be made by nanoengineered particles of hydroxyapatites for biomedical applications such inhibition and prevention of bacterial infections. The project will test methods to synthesis silver doped hydroxyapatite and collagen/silver doped hydroxyapatite able to inhibit the bacterial activity of different species of bacteria in the laboratory that will be implemented in real infected sites.

The decisive aim of this project is to develop specific nanoparticles/composites to prevent and remediate bacterial infections, without using antibiotics. For this reason, the new biomaterial based on nanoparticles of hydroxyapatite doped with silver and collagen/silver doped hydroxyapatites composites,will have antimicrobial properties due to their characteristics, but there is no risk to develop bacterial resistance.

Innovative nanostructured materials and coatings with antimicrobial activity for medical applications Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-1292 2014 - 2017

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU INGINERIE ELECTRICA ICPE - CA BUCURESTI

Project partners: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU INGINERIE ELECTRICA ICPE - CA BUCURESTI (RO); UNIVERSITATEA BUCURESTI (RO); MGM STAR CONSTRUCT S.R.L. (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.icpe-ca.ro/proiecte/proiecte-nationale/pn-2014/inmatco.pdf

Abstract:

The project aims to develop novel products and knowledge in antimicrobial nanostructured coatings for medical applications like critical surgical instruments (scalpels, knives, forceps and scissors) to reduce superficial and deep surgical site infections and to improve quality of life. Nanostructured materials of MeNPs/MeONPs type (MeNPs = Ag, Cu, MeONPs = CaO, MgO, ZnO, TiO2) with average MeNPs size of 10-20 nm and average MeONPs size of 30-50 nm) and high antimicrobial activity will be obtained by an innovative chemical synthesis of colloidal suspensions containing ecological NPs with 0.1-0.5 wt.% MeNPs and 1-3 wt.% MeONPs. Chemical deposition of MeNPs on MeONPs supports will be realized by a self-assembled nanolayers coating process, being a new and efficient approach in control of NPs size and stability. A higher content of MeNPs will be obtained in comparison with the existent solutions. The MeNPs and MeONPs mixtures will show synergistic effects of antimicrobial properties compared with those of MeNPs and MeONPs used alone. Ecological and efficient processing methods will be used to obtain and deposit the bioactive agents designed to inhibit the adhesion of microorganisms on stainless steel supports that show lack of antibacterial properties. The MeNPs/MeONPs composite powders will be deposited by different methods such as Thermal Evaporation, EBPVD (e-gun), Sputtering Deposition (Magnetron) and Arc-PVD (Plasmotron) on the metallic substrates with ensuring a good adhesion without changing the substrates original properties. Nanostructured coatings with homogenous and dense surface without any faults (pinholes and cracks) will be achieved at low temperatures to not affect the materials properties. Optimized coatings will be developed by a graded structure and adjusting stress level. Other original and innovative aspect is related by sputtering targets development from selected nanostructured materials by spark plasma sintering (SPS) process, where nanostructure features are maintained since grain growth and the development of equilibrium states are suppressed. The project addresses to the priority thematic of the PARTNERSHIPS PN-II-PT-PCCA-2013-4 program, Domain 7. “Materials, processes and innovative products“, Research field 7.1 Advanced materials, Thematic Area 7.1.6. “Advanced materials and biomaterials for health quality increasing“ with a direct impact in Domain 4. “Health” and European Research Area (ERA)-Nanomaterials. The project has inter-disciplinary, multi-disciplinary and trans-disciplinary nature being carried out by high skilled specialists with complementary expertise. Certified prototypes and end-products of critical stainless steel surgical instruments coated with antimicrobial MeNPs/MeONPs nanostructured materials with uniform and homogeneous mono/multilayer with thickness ranging 0.1-50 µm will be obtained. The coatings will have high resistance to mechanical wear, high resistance to heat stresses, high hardness, low friction coefficient and good adhesion to substrate. The novel products will be introduced on the market only after their certification granted by the Health Ministry from Romania. The coated products will be at a competitive cost with uncoated ones, contributing to the requests of the global and national socio-economic environment in the field of advanced materials and health with a major impact in reducing surgical site infections. Knowledge protection will be made by two patent applications registered to the national State Office for Inventions and Trademarks (OSIM). Results dissemination will be made on a large scale by publishing scientific papers in ISI ranked journals, attendance at national and international events, setting up and updating project web site, elaboration of dissemination materials.

Innovative antibacterial and self-cleaning photocatalytic textiles Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0419 2014 - 2017

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU TEXTILE SI PIELARIE - INCDTP BUCURESTI

Project partners: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU TEXTILE SI PIELARIE - INCDTP BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA (RO); UNIVERSITATEA BUCURESTI (RO); STOFE BUHUSI S.A. (RO); C & A COMPANY IMPEX SRL (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://cleantexproject.ro/

Abstract:

The negative impact of pollution on human health and environment pushed the R&D efforts to develop clean technologies and products according to the green chemistry principles, making regulation, control, clean-up and remediation unnecessary.

The project’ target is the development of new multifunctional textiles providing simultaneously photocatalytic, auto-sterilizing, self-cleaning and enhanced antimicrobial properties based on innovative graphene oxide/TiO2 nanocomposites able to decompose pollutants in safe, non-toxic compounds, using only solar light.

The present project aims at developing the 4th generation of green photocatalysts by:

• synthesis of graphene oxide/doped titanium oxide (GOT) with efficient absorption under UV and visible light

• formulation of GOT composites as highly adherent solution;

• development of photocatalytic textiles by deposition of photocatalytic compounds in one-step technology;

• evaluation of photocatalytic/self-cleaning/antimicrobial performances of synthesized photocatalysts and textiles against usual pollutants and pathogenic microorganisms,

• biocompatibility/cytotoxicity testing of cells cultures toward photocatalytic compounds.

The original contribution of the project consists in:

• optimal assembly and interfacial coupling of the TiO2 nanoparticles over the graphene oxide sheets;

• innovative adhesive graphene oxide/TiO2 (GOT) formulations, ensuring a high and stable dispersion and a strong adherence of the composites to the textile substrate, while preserving the genuine physical and mechanical properties of textile;

• homogeneously and firmly adherent photocatalytic coatings by one step deposition of GOT at room temperature, reducing the raw materials, utilities and manpower consumption;

• new investigations on graphene/TiO2 cytotoxicity and biocompatibiliy.

The indicators proposed to be achieved:

- eco-friendly products: minimum 2 types of composites nanopowders; min. 2 types of textile materials with high photocatalytic and antibacterial efficacy; self-cleaning and antibacterial work wear and protective equipment; clean technologies: one step synthesis of powder composites; one step deposition and fixation of synthesized compounds on textiles;

- innovations: 1 patent describing the innovative GOT synthesis and one-step deposition of nanocomposites; minimum 2 ISI rated scientific papers, minimum 2 presentations at national and international conferences.

The innovative approach for the production of high quality photocatalytic textiles is based on an efficient, environmentally clean and easily implementable at industrial-scale process, one step deposition and fixation of environmentally friendly nano composites. This finishing technology combined with a proper adhesive photocatalysts formulation will eliminate the post-treatment steps, and consequently high consumption of chemicals, water and energy and will allow the achievement of highly adherent, durable, uniform coating thin layers preserving the genuine physical properties and colour of the textiles. The easy application on conventionally existing production lines, ensure a wide spread of the finishing technology. The approval of the present project will contribute to concentration of human and material resources with the aim to achieve the above mentioned research tasks and the implementation of results in Romania, with special amelioration of the economic situation of SMEs active in textiles and chemical industry and significant improvements of human health and environment quality.

Inovative, multidisciplinary research to investigate the probiotic effects of new lactic acid bacteria strains and consortia Call name: Joint Applied Research Projects - PCCA-2011 call, Type 1
PN-II-PT-PCCA-2011-3.1-0969 2012 - 2016

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL DE BIOLOGIE (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); UNIVERSITATEA DE STIINTE AGRONOMICE SI MEDICINA VETERINARA (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://genetmicroproiecte.blogspot.ro/search/label/PROLAB%2077

Abstract:

The emergence of bacterial resistance and multiresistance to antibiotics represent a major public health problem in Romania and also in the entire world. In Romania, there is an increasing trend of the incidence of infections with bacterial strains highly resistant to antibiotics, whose prognosis is often worsened by the producing of bacterial biofilms on the tissues and biomaterials used in medicine. In our country, the problem of development of some alternative strategies for the prevention and treatment of the infections with multiresistant and biofilm forming bacteria is less studied, despite their great application potential in biomedicine and their utility in the recovery of the balanced or eubiosis state of the normal microbiota, after the negative impact of the antibiotics on bacterial communities. One of the most viable alternatives is represented by probiotics products which by administration in adequate amounts confer a health benefit on human organism. For the best use of the probiotic microorganisms, the mechanisms by which they work should be better understood. The selection of an appropriate probiotic strain for its inclusion in a probiotic preparation should be made on the basis of its capacity to induce an improved host response without modification of the intestinal homeostasis.

The main goal of this project imply complex studies of probiotic strains (lactic acid bacteria newly isolated) and multispecific consortium in order to improve the experimental protocol for the optimal selection of human use probiotics, based on their anti-infective and immunomodulatory activities.

Novel nanostructured prosthetic tubular devices with antibacterial and antibiofilm properties induced by physico-chemical and morphological changes Call name: Joint Applied Research Projects - PCCA-2011 call, Type 2
PN-II-PT-PCCA-2011-3.2-0284 2012 - 2016

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); I.C.P.E. BISTRITA S.A. (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.antibiotube.ficai.eu

Abstract:

The project is intended to overcome the bacterial colonization of the prosthetic tubular devices, this being one of the most common reason of the tubular devices failures. The main objectives of the project include the surface modifications of the existing prosthetic tubular devices but, also bulk modifications will be tested, especially from the point of view of drug release properties. As surface modifications techniques, chemically modifications will be tested because it allows strong interactions between substrate and coating. Based on the existing expertise of P1-INCERPLAST, the only Romanian prosthetic tubular devices manufacturer, the PVC based devices will be used as substrate. The surface modifications will involve the partial substitution of Cl with HO followed by different chemical reaction: 1) alkylation/acylation when hydrophobic groups are intended to be attached on the surface of the tubular devices (in this way, the adhesion of bacteria is not favored); 2) thin polyurethane or silicon layer deposition starting from synthesized prepolymers, the withdrawal reaction being between prepolymer and hydroxyl groups of partially substituted PVC support; 3)coating of the tubular devices surfaces with antimicrobial nanoparticles, the stability of the coating being improved by proper functionalization of the substrate (if nanoAg are intended to be deposited on the PVC tubular devices, chloride will be partially substituted with SH; 4) the tubular prosthetic devices will be impregnated with antimicrobial/antibiofilm/anti-fouling agents, chemical bonding being prefered; and 5) new tubular devices will be obtained, the wall acting as a support for drug delivery. All these devices will be carefully selected and will be the subject of various scientific publications and patents. The ultimate goal of the project is the CE certification and finally their commercialization.

FILE DESCRIPTION	DOCUMENT
List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
R&D activities in enterprises
Peer-review activity for international programs/projects