BrainMap

Gabriela Liliana Ailiesei

- INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI"

Expertise & keywords

Integrated use of the next generation plant biostimulants for an enhanced sustainability of field vegetable high residue farming systems Call name: EEA Grants - Proiecte Colaborative de Cercetare
RO-NO-2019-0540 2020 - 2024

Role in this project:

Coordinating institution: Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti

Project partners: Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO); Norwegian Institute for Water Research (NO); INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI" (RO); Norgenotech AS (NO); ENPRO SOCTECH COM SRL (RO); AMIA INTERNATIONAL IMPORT EXPORT S.R.L. (RO)

Affiliation: INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI" (RO)

Project website: https://icechim.ro/project/stim4plus-en/

Abstract:

The project addresses mainly the thematic area of Biotechnology, more exactly the key topic Biotechnology for agriculture, aquaculture, forestry and biomass production. The biotechnological solutions which STIM4+ project proposes to develop are intended to compensate the negative effects of the low-input, high-residue sustainable vegetable production systems. These proposed biotechnological solutions are related to the use of the next generation plant biostimulants. Plant biostimulants represent an emerging class of agricultural input, which protect plants against abiotic stress, enhance / benefits nutrients uptake and improve yield quality . The proposed plant biostimulants to be used for an enhanced sustainability of field vegetables grown in a high residue system are including into all classes / subclasses mentioned in the new EU Regulation 1009/2019. The multi-functional Trichoderma strains-based plant biostimulants (a microbial plant biostimulants) will be included into a glycodinameric, chitosan based bioactive (micro)hydrogel formulation. Decoration of microgels with anchor peptides will be used as a (micro)hydrogel tackifier on the plant residues. The bioactive hydrogel is based on an organic plant biostimulants (chitosan), which will be used to generate a biocompatible 3D porous structure, thermo- and pH-responsive and with a hydrophilic – hydrophobic segregation feature. This hydrophilic – hydrophobic segregation (micro)hydrogels will be used for embedding hydrophobic mimetic strigolactones. Propper application technologies of such smart formulated hydrophobic molecules will be developed, to exploit strigolactones both functions, as exo-signals for a better harnessing of beneficial microbiome and as cue for deleterious organisms (e.g. to induce suicidal germination of parasitic plants). The smart formulated bioproducts / agricultural inputs and the agricultural practices intended to exploit their specific features of such bioproducts are an example of biotechnologies for agriculture. The natural strigolactones mimics will be a part of the microbial standardized extract, which we intend to produce from microalgae culture and which is another example of organic plant biostimulant. This microbial standardized extract will include natural strigolactones, polyamines and betaines. Both strigolactones and polyamines are exo- and endo-signals. As exo-signals, both strigolactones and polyamines have been demonstrated to enhance mycorrhizae hyphal branching and root colonization. As endo-signals, both strigolactones and polyamines are involved in plant stress responses. Betaine also supports plant response to stress, especially to drought. Strigolactone mimics used for laboratory screening will be synthetized based on a rational bio-design. The inorganic plant biostimulants are represented by selenium, as zerovalent nano-selenium. A large body of evidence demonstrates that selenium acts as a plant biostimulant. Selenium protects plants against abiotic stress, especially drought, enhances / benefits nutrient uptake and improves edible yield quality. Nanoselenium (zerovalent) particles show a much lower environmental impact and an improved efficiency compared to other selenium species. Application of selenium nanoparticles reduces the risk of accumulation of polyamines in the edible yield. From a food safety point-of-view, accumulation of polyamines in vegetables grown into HV mulch could have some carcinogenic effects, because polyamines were found to support proliferation of various tumor cells.

The project is an interdisciplinary one and address also other key topics as Environmental impact and risk assessment of the modern, new and emerging technologies and products. Safety and environmental impact of the new developed products will be determined by a state-of-art 3R techniques, by Norway partners. The project contributes to the objectives and the priorities of the call. It supports research cooperation between Romania and Norway and consolidate a strategic partnership.

Mimicking living matter mechanisms by five-dimensional chemistry approaches Call name: P 4 - Proiecte Complexe de Cercetare de Frontieră
PN-III-P4-ID-PCCF-2016-0050 2018 - 2022

Role in this project:

Coordinating institution: INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI"

Project partners: INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI" (RO); INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI" (RO); INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI" (RO); INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI" (RO); INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI" (RO); INSTITUTUL DE BIOLOGIE SI PATOLOGIE CELULARA ,,NICOLAE SIMIONESCU'' (RO); CENTRUL DE CHIMIE ORGANICA AL ACADEMIEI ROMANE "C.D.NENITESCU" (RO)

Affiliation: INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI" (RO)

Project website: http://www.intelcentru.ro/5D-nanoP/

Abstract:

Mimicking the living matter mechanism of cooperation by complementarity represents one of the most challenging tasks of supramolecular chemistry. For now the solution consists in using particularly designed molecular unimers, endowed with the necessary amount of chemical information.

The 5D-nanoP project is dedicated to interfacing the fundamental research area of constitutional dynamic chemistry with the practical approaches of medicinal chemistry and biomedical applications. In the spirit of a metaphor of Jean-Marie Lehn (Nobel Prize in Chemistry, 1987), the project aims to materialize the concept of 5D chemistry in designing, synthesizing, characterizing, and using molecules with conditional affinity, to build versatile supramolecular nanoplatforms able to vectorize compounds of pharmaceutical or biochemical relevance, and genetic actuators, all of them involved in physiologic and pathologic processes at cell- and tissue-level.

The project will add the layer of 5D chemistry over the backgrounds of molecular assembling line techniques to produce particulate nanoplatforms, self-assemblable in the virtue of the chemical information stored by the designed unimer molecules. Two modern techniques of building dynamic chemical structures will be considered: (i) the use of self-immolative linkers, and (ii) the space stepwise and time phased (microfluidic) assisted synthesis. To prove the applicability of the produced nanoplatforms, an ex vivo cell cultivation system will be developed to emulate tissue/tumor niches.

Eight teams will be involved in the 5D-nanoP project to cover the main addressed research areas: (i) the in silico molecular design, (ii) the development of a unimers chemical library, (iii) the development of a molecular assembling line, (iv) the conjugation of the developed platforms with chemical species of biomedical interest, (v) the build of ex vivo emulating niches, and (vi) the bio-oriented assessment of nanoconstructs efficacy.

Closing the bioeconomy value chains by manufacturing market demanded innovative bioproducts Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0569 2018 - 2021

Role in this project:

Coordinating institution: Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti

Project partners: Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO); INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI" (RO); UNIVERSITATEA "DUNAREA DE JOS" (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); UNIVERSITATEA AUREL VLAICU ARAD (RO)

Affiliation: INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI" (RO)

Project website: http://icechim-rezultate.ro/proiect.php?id=51&lang=ro

Abstract:

Agriculture and food industry in Romania generates large amounts of co/by-products, which are not used and turn into wastes, with negative impacts. The approach of the project PRO-SPER is to develop integrated processes, flexible and interconnected, to transform a number of agro-food by-products in bio-products, with market demand. This approach facilitates the achievement of project goals, complex-coordination and linking of the research organizations that are members of the Consortium, INCDCP-ICMPP, UDJ, ICECHIM, INCDSB and UAV, for improving their institutional performance in the field of nano-and bio-technologies of their application into bioeconomy.

The overall objective of the project PRO-SPER is to increase the impact of research and development activities and innovation of the RDI institutions, by developing and harnessing innovative technological solutions for bio-nano-processing of several by-products from the bioeconomy value chains, for recovering and/or formation of value-added components and their use in order to obtain products with high added value.

Expected results through the implementation of the project (21 new jobs, 23 national patent applications and international patent applications 6 EPO/WIPO; 10 technologies/new products resulting from the project, at a level of technological maturity to enable taking over by the operators, 5 services research and technological research services 10 cheques, 10 experiments cheques services

80 internship of young researchers from and within partner institutions, 50 visits for developing new techniques for working jointly in the Consortium, 30 training internships for new employees, 28scientific papers, 1 joint program CDI, in line with the development plan of institutional partners) have a significant impact on the capacity-building of the partners in the Consortium.

A next generation plant biostimulant based on strigolactones included into stimuli responsive nanoformulation Call name: P 3 - SP 3.2 - Proiecte ERA.NET
ERANET-INCOMERA-BENDIS 2018 - 2019

Role in this project:

Coordinating institution: Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti

Project partners: Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO); INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI" (RO); ENPRO SOCTECH COM SRL (RO)

Affiliation: INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI" (RO)

Project website: http://icechim-rezultate.ro/proiect.php?id=50&lang=ro

Abstract:

The BENDIS project aims to integrate technologies for the industrial production of a next generation plant biostimulant based on strigolactones (SL) analogs and/or mimics, included into a stimulus-controlled release nanoformulation. A better harnessing of exogenously applied SL analogues/mimics requires such formulations, which protect SL from rapid degradation and promote their steady release at the biologically active concentrations under controlled conditions – i.e. roots mediated pH changes in the soil. The nanoformulation includes mesoporous nanosilica, loaded with a hydrophobic "green" solvent with dissolved SL analogues/mimics. Mesoporous nanosilica particles are decorated with humic acids, wherein SL are embedded. Humic acids are immobilized on mesoporous silica through an aminopropyl bonding.

Development of a functional model for sustainable capitalization of genetic and phytochemical diversity of Arnica montana L. wild populations in the Northern area of the Romanian Eastern Carpathians Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0791 2014 - 2017

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI" (RO); UNIVERSITATEA "ALEXANDRU IOAN CUZA" IASI (RO); UNIVERSITATEA "ŞTEFAN CEL MARE" DIN SUCEAVA (RO); CENTRUL DE CERCETARE SI PRELUCRARE A PLANTELOR MEDICINALE PLANTAVOREL SA (RO)

Affiliation: INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI" (RO)

Project website: http://ccb-stejarul.ro/2014/ARMOREC.html

Abstract:

The ARMOREC project has as the general aim the optimizing the capitalization of the Arnica montana L. natural populations from the north of the Eastern Carpathians by developing a new innovative model for the sustainable production of raw material (collecting, cultivation, conditioning) and of some eco-efficient processing methods of the vegetal material.

In this respect, it is necessary to develop inter- and transdisciplinary studies in the fields of biology, ecology, geomorphology, biotechnology and phytochemistry, grouped in 3 major components: the environment componen with connex aspects, the phytochemical and economical component – the capitalization by the industrial partner (SME).

The members of the concortium, by their expertise and experience in the project domain has the competences necesary to the development of the researches with a high degree of complexity and interdisciplinarity.

To attain the general objective of the project, we will process a series of activities grouped in 5 work packages corresponding the specific objectives:

1. Evaluating the bioproductive potential in the natural populations of A. montana from the north of thr Eastern Carpathians, on the basis of the quantitative and qualitative determinations onthe biomass yield, as well as the content of biologically active principles.

2. Evaluating the preservation status of the A. montana species in the target area, correlated to the main pressure factots.

3. Elaboration of a model of developing a coplimentary source of raw material by conventional and biotechnological methods.

4. The development of some eco-efficient methods of extraction and phytochemical anaysis for the main biologically avtive compounds – sequiterpen-lactons, flavonoids and phenolic acids, essential oil.

5. The development of an experimental model for the sustainable capitalization of A. montana (natural populations and experimenta cultures) by obtaining and characvterizing the specific phytocomplexes with multiple uses.

The originality of the project resides both in selecting the study and analysis methods and in the holistic approach of the problem that aim the whole capiatlizing chain of the principles of sustainable growth. It is the first time when an interdisciplinary approached is proposed to model the effect of the abiotic fators on the distribution of A.montana on the basis of a spacial analysis model.

Reaching the aims will promote the sustainable capitalization of the species in the north of the Eastern Carpathians, that will have complex , interdisciplinary stadie at the basis (with economic and scientific importance) and will generate added value over the whole chain of capitalization and will also assure the biodiversity conservation.

Although for A. montana he interst kept itsel at a high international level, in România, the species is slightly capitalized, both economically and scientifically. Thus, thorough studies are scrace, integrated in the present state of the species in the natural populations, in the preservation and capitalizing and promoting of autochthonous products. The majority of the studies achieved in Romania refer to the Apuseni Mountains, in the Eastern Carpathians, the species being only mentioned in some floristic studies.

By developing this project, we aim the sustainable capitalization of the species at local and regional level, in relation to the peculiarities of the species, and of its status as a rare and vulnerable species and the demands of the market.

Approaching a species that has already for long been studied worldwide, represents a reason to elaborate a capitalization plan of the species, this being in the same time a very good model to capitalize other medicinal, aromatic and food species of the mountain zone. In the time, this model of sustainable capitalization may be adapted and extrapolated to all the Romanian areals of this species.

Nanosized micelles and vesicles from amphiphilic block copolymers with polysaccharides as hydrophilic blocks. A versatile route to new biomaterials Call name: Exploratory Research Projects - PCE-2011 call
PN-II-ID-PCE-2011-3-0622 2011 - 2016

Role in this project:

Coordinating institution: Institutul de Chimie Macromoleculara "Petru Poni"

Project partners: Institutul de Chimie Macromoleculara "Petru Poni" (RO)

Affiliation: Institutul de Chimie Macromoleculara "Petru Poni" (RO)

Project website: http://www.icmpp.ro/nichifor0622

Abstract:

The ability of some amphiphilic block copolymers to self-organize in aqueous media is a nowadays approach to development of new materials of high complexity, with unique properties and numerous practical applications. The aim of the project is to synthesize amphiphilic block copolymers with chemical structures controlled by synthesis, from polysaccharides (dextran, pullulan) as hydrophilic blocks and biodegradable polyesters of bile acids (cholic, deoxycholic or litocolic acids) as hydrophobic blocks. Double hydrophilic block copolymers of polysaccharides and poly(isopropylacrylamide) with capacity to self-assembly only above their low critical solution temperature will also be prepared. The polymer characterisitics (nature and length of the blocks) and medium properties (solvent composition, pH, temperature, ionic strength) determining the occurrence of nano-sized organized structures (micelles and/or vesicles) and their properties (size, shape, morphology, stability, encapsulation ability for hydrophobic or hydrophilic compounds) will be preponderantly followed. In order to improve their properties and suitability as devices for temporal and spatial control of drugs, the obtained nanoparticle will be further functionalized by crosslinking, modification with ionic or hydrophobic groups, and attachment of targeting moieties (folic acid). Single and double-drug encapsulation and release ability, biocompatibility and interaction with specific cells will be also examined.

FILE DESCRIPTION	DOCUMENT
List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
R&D activities in enterprises
Peer-review activity for international programs/projects