BrainMap

Mr. Gabriel Lucian Radu

Prof.dr.eng.; Doctor Honoris Causa

Researcher - INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Other affiliations

Professor - UNIVERSITATEA NAȚIONALĂ DE ȘTIINȚĂ ȘI TEHNOLOGIE POLITEHNICA BUCUREȘTI (Romania)

Researcher | Teaching staff | Scientific reviewer | PhD supervisor

Web of Science ResearcherID: E-8560-2011

Curriculum Vitae (10/09/2021)

Expertise & keywords

Nanocrystalline Graphene e-Tongue for Extra-Virgin Olive Oils Testing Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-2256 2022 - 2024

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); INSTITUTUL NATIONAL DE CERCETARE- DEZVOLTARE PENTRU MICROTEHNOLOGIE - IMT BUCURESTI INCD (RO)

Affiliation:

Project website: https://www.imt.ro/NAGET/rezultate.php

Abstract:

Olive oil is one of the most valuable foods due to its unique organoleptic properties, its high content of monounsaturated fatty acids (70-80%) and its antioxidant properties given by the presence of lipophilic phenols (tocopherols) and hydrophilic phenols (hydroxytyrosol, tyrosol, oleacein, vanillic acid) in the composition of olive oil. Extra virgin olive oil (EVOO) is traded at higher prices than other vegetable oils due to its exceptional properties such as nutritional value, aroma and excellent taste and is therefore subject to fraudulent activities. Therefore, the present project proposal aims to develop an electrochemical tongue sensor paired with chemometrics targeting the adulteration of extra-virgin olive oils. The proposed analytical approach being developed will be validated in relation to a standardised method used to determine the authenticity of extra virgin olive oils. The electrochemical tool will benefit from the knowledge already acquired by the consortium members. The e-tongue will consist of a suitable conductive material as the sensing interface (i.e. nanocrystalline graphene (NCG) based electrodes), the surface of the transducers will be modified with graphene quantum dots (GQDs) to chemically tailor the surface, increase the electroactive area and improve the sensitivity. The electrochemically recorded data will be processed and analysed using established and new chemometrics tools to train and validate a method for classifying olive and vegetable oils and another for predicting the concentrations of selected markers associated with olive oil adulteration.

Phytonutrients based on phytocomplecs enriched in phytoestrogens with potential for use in the management of metabolic diseases Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-1185 2022 - 2024

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); CENTRUL DE CERCETARE SI PRELUCRARE A PLANTELOR MEDICINALE PLANTAVOREL SA (RO)

Affiliation:

Project website: https://www.incdsb.ro/p/Phyto-ESM/

Abstract:

Nowadays, obesity and diabetes mellitus are two major nutritional disorders due to their frequent association with major cardiovascular risk factors, responsible for increasing mortality. Recent studies have shown that phytoestrogens have various beneficial effects on health by reducing the risk of cardiovascular disease, obesity, metabolic syndrome and type 2 diabetes and different form of cancers.

This project aim is technology for obtaining phytonutrients based on phytocomplexes concentrated in phytoestrogenic compounds from Trigonella foenum-graecum, Medicago sativa and Solidago virgaurea and evaluation of their effect on key enzyme linked to type-2 diabetes and obesity and also testing the biological effects using in vitro cell culture. The project technological development will foresee: a) Protocols for optimum extraction process, using modern extraction technologies: microwave assisted extraction, ultrasound-assisted extraction and accelerated solvent extraction of phytoestrogenic compounds; b) Achievement and validation of innovative laboratory technology based on selective separation processes (microfiltration/ultrafiltration for preconcentration and nanofiltration for phytoestrogens concentration); c) Analytical characterization and testing the biological effects of phytocomplexes enriched in phytoestrogens.

Involving a brand company in the domestic and foreign market in medicinal plants processing will lead to increasing the potential of the private company to get involved at performance level in scientific and technical and to develop new nutraceuticals, herbal drugs or food products for prevent of metabolic diseases.

The original results on the laboratory technology will be patented for transfer to the partner SME.

DEVELOPMENT OF INSTRUMENTS AND STRATEGIES FOR HIGHER VALORIZATION OF NATURAL RESOURCES FOR IMPROVING THEIR SUSTAINABLE EXPLOITATION IN BIO-INDUSTRIES AND CONSERVATION OF ECOSYSTEM FUNCTIONS Call name:
PN III 1927 2019 - 2022

Role in this project: Project coordinator

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE ()

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE ()

Project website: http://www.incdsb.ro/en/proiecte/proiect-nucleu/

Abstract:

Obtaining, physico-chemical and biological characterization of composite biomaterials and selection of the optimal biomimetic variants of dermal extracellular matrix; in vitro biological characterization of composite polymeric biomaterials by testing on specific cells of dermal tissue

Development of New PHYTo-formulations with enhanced BIOACTive potential based on polyphenols complexes from plants and vegetal by-products Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-3945 2020 - 2022

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); UNIVERSITATEA BUCURESTI (RO); CENTRUL DE CERCETARE SI PRELUCRARE A PLANTELOR MEDICINALE PLANTAVOREL SA (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: https://www.incdsb.ro/p/nphytbioact/Rezultate.html

Abstract:

NPHYTBIOACT project intends to demonstrate the feasibility of using innovative technologies to improve natural resources (plants and vegetal by-products) use and to increase the efficiency of phyto-products formulation. The core objective of the project: to settle a complete solution for development and lab-scale validation of new phyto-formulations with enhanced bioactivity. Achieving the NPHYTBIOACT major goal implies the following technological downstream: 1.Validation of the innovative technologies of extraction existing at the consortium level (TRL 3) for silymarin extraction from milk thistle and polyphenols/flavonoids extraction from rosehip; 2.Development and characterization of phytosomes (phyto-phospholipids complexes) based on complexes “active compound –phospholipids” to be further used in phyto-products formulation. In our case active compound are silymarin, respectively polyphenols/flavonoids while phospholipids are phosphatidylcholine, phosphatidylethanolamine phosphatidylserine; 3. Lab-scale new phyto-formulations based on the developed phytosomes & validation of the new-formulations

At the project completion the following outcomes will be provided: two validated extraction technologies (microwave assisted extraction), for silymarin from milk thistle (plant, by products) and polyphenols/flavonoids from rosehip (plant, young branches, by-products) and selective concentration and standardization in rutin, quercitrin and kaempherol will be performed; lab-scale validated procedures for phytosomes synthesis, silymarin-phytosomes, respectively flavonoids-phytosomes; validated formula for new highly bioactive phyto-products including synthesized phytosomes.

NPHYTBIOACT, aims to perform a step forward from the results existing at the consortium level, targeting to improve the existing know-how in order to obtain new phyto-products with enhanced bioactivity and increased solubility characteristics in physiological relevant fluids.

Metode inovative de valorificare a resurselor naturale si de imbunatatire a eficientei nutritionale a fito-produselor - contributii la cresterea competitivitatii micilor intreprinderi Call name:
2016 - 2021

Role in this project: Project coordinator

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE ()

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE ()

Project website: http://www.incdsb.ro/p/FITOCOMP/index.html

Abstract:

Innovative methods for natural resources high valorisation and improving the nutritional efficiency of phyto products – knowledge support to increase the competitiveness of small enterprises

Buget 9015935 RON (1930607 Eur)

EU share (%) 83.72 %

Implemenmtation period (years): 2016-2021 (5 years)

Implementer (type): Public institution

Type of investment: Public and private

Operational objective Operational Program Competitiveness, axis: Research, Technological Development and Innovation (RDI) to support the economic competitiveness and business development

Expected direct results:

a.Knowledge transfer results

a1.Innovative solutions for phyto-products/phyto-drugs technologies; technological process innovation ensuring higher exploitation of natural resources (plants, fruits, biomass);

a2. Knowledge transfer portofolio including:

18 methods of characterisarion, efficiency assessement and quality control of vegetal material and formulated phyto-drugs;

3 new extraction technologies applicable to raw materials and by-products;

6 new customized instruments for fast analisys & quality control of agro-food/phyto-pharmaceutical products;

a3.Knowledge transfer online platform FITO-PLAT connecting experts, industrial end-users, technology brokers, regulatory organisations;

b.Human resources results

b1. 2 new available positions for specialists in knowledge transfer; technology brokers, innovation management;

b2. Increased professional capabilities of the implementation team by widening industrial experience and technology transfer experience as results of two instruments, professional formation and direct assistance in technology implementation at facilities of SME partners;

c.Increased number of direct contracts with SME providing direct servicies (45 new contracts), new implemented technologies (3 new contracts with SME’s from phyto-pharmaceutical industry, nutritional suplements industry and food/beverages industry) and food/beverages industry)

Expected outputs / deliverables:

The use of new technologies by the SMEs as recipient of the knowledge transfer will determine the optimization of the natural resources use by the beneficiary SME eliminating their dependence on the optimal harvesting periods and ensuring the continuity of the technological process line, accordingly contributing to the realization of a new value chain.

Direct assistance to increase the competitivness of at least 3 SME from Romanian agro-food industry, producing and promoting nutritional supplements, beverages and phyto-drugs, as recipients of knowledge transfer

Reducing the direct production pressure for existing technological lines at recipient SMEs by implementation of new control instrumenst and new valorisation technologies (for by-products)

INNOVATIVE TECHNOLOGY FOR ASSESSING THE PERIODONTAL DISEASE AND NEW PERIODONTITIS TREATMENT BASED ON HYALURONIC ACID AND MELATONINE Call name: P 3 - SP 3.2 - Proiecte ERA.NET - COFUND
COFUND-MANUNET III-HAMELDENT 2018 - 2020

Role in this project:

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); CONCORDIA DENT SRL (RO)

Affiliation: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Project website: http://www.hameldent.ro

Abstract:

Nowadays severe periodontal disease is found in 15–20% of middle-aged adults. Local application of antiseptics and antibiotics has been advocated for the treatment of localized periodontitis lesions with modest improvements in probing-depth reductions. The levels of inorganic ions (Na,K,Ca,Mg) in saliva of periodontitis patients is a potential periodontal marker by their correlation with periodontal parameters but not always their values were conclusive. We propose a clinical device based on sensors technology able to determine the level of such electrolytes directly in the periodontal pocket which we expect to offer an improved evaluation of ions level and further correlation with periodontal disease evolution. Also we propose a new biocompatible matrix: melatonin, hyaluronic acid and antibiotic for insertion in the periodontal pockets. As result is possible to foreseen more effective methods for periodontitis treatment and prevention. HAMELDENT project scope is to develop: a new material for topical treatment of the periodontal disease and a clinical device able to determine the level of inorganic ions from periodontal pocket as quick assessment tool of the periodontal disease.

Pencil˗based sensor array for naringenin like compounds analysis from beverages Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-0477 2017 - 2018

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://www.chimie.unibuc.ro/cercetare/analitica/PED_2017_David_Iulia.pdf

Abstract:

The health benefits of phenolic antioxidants from food and beverages is well known. At present, there are no specific legislative or regulatory requirements with respect to antioxidant capacity of a compound nor a standard method for naringenine (NG) and derivatives quantification. Flavonoid antioxidants of NG and derivatives type exist in different concentrations in largely consumed beverages (beer, juice, tea) worldwide. Therefore, there is a great demand on cheap and non-polluting methods for rapid, routine determination of the composition of complex samples with implications on human health. The aim of the present project is the development and validation of a method for NG and derivatives determination and of a dual sensors array based on disposable pencil graphite electrode (PGE) which has good electrode characteristics, is non-toxic, cheap, easy to procure, being largely commercially available. The device intended to be developed and validated consists of a bare PGE for the content and a DPPH-modified –PGE for the antioxidant efficacy assessment of NG and derivatives from beverages, using methods already developed by project team members (coordinator-UB has experience in voltammetric methods development based on PGE; partner P1- NIRDBS has expertise in methods and sensors development pot the assessment of antioxidant efficacy of compounds from different matrixes). According to the existing team members’ publications, this proposal starts from TRL2 and reaches TRL4 after the development and validation of the dual sensors array. The proposed device could be used as a reliable and rapid tool for beverages process control in order to replace tedious and time-consuming analysis, if yes/no decisions should be taken.

Fast analysis method of aspartame from foods using an amperometric enzymatic biosensor Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-0503 2017 - 2018

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://www.incdsb.ro/p/Aspsense/PED-180-ASPSENSE.htm

Abstract:

This demonstrative project aims to develop and validate in laboratory a fast analytical method for detection of aspartame from food samples based on a combination of an amperometric enzymatic biosensor and a Low-noise potentiostat. The growing use of aspartame in the food industry requires the development of rapid and effective methods for its determination. The project aims to provide a cost effective and easy to use analytical method of aspartame. The detection principle of the biosensor is based on enzymatic oxidation of aspartame catalyzed by carboxyl esterase (CaE) and alcohol oxidize (AOX), with the production of hydrogen peroxide that is electrochemically quantified. The developed potentiostat used for the quantification of the biosensors’ amperometric signals has two building blocks: (i) a potentiostat circuit that provides constant continuous voltage and (ii) current sensing circuit to quantify the current solution ICELL related with the concentration of aspartame. Low-pass filter will be added to the current sensing circuit’s feedback loop to diminish the induced electromagnetic noise effects. The consortium combines the vast experience in the field of biosensors and enzymology of National Institute of R&D for Biological Sciences with the know-how in electronic potentiostat design of the University Politehnica of Bucharest.

High Valorisation of Press Cake Resulted in Processing of Milk Thistle Oil by Cold Pressing Call name: P 2 - SP 2.1 - Transfer de cunoaștere la agentul economic „Bridge Grant”
PN-III-P2-2.1-BG-2016-0119 2016 - 2018

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); HOFIGAL EXPORT IMPORT SA (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: https://www.chimie.unibuc.ro/cercetare/analitica/VALSUSA.pdf/

Abstract:

The project is targeting two main objectives: a) development of efficient extraction technologies to support the higher valorisation of byproduct resulted from milk thistle processing to obtain oil; b) strenghtening the collaboration between academic research and industrial end-users and enhancing the human resource expertize. Achieving the first objective involves the following subsidiary objectives: characterization of by-product from the production of oil thistle in terms of profile composition and initial efficacy; development and optimization of extraction technologies; formulation and characterization of extracts with high content of sylimarin; demonstration/testing/validation of realised extraction technologies for the trader. At the end the obtained product have to have a silymarin concentration of at least 50%. The second objective consists of training internships of master students at the trader facilities, to enhance their entrepreneurial capabilities, and the training of specialized HOFIGAL staff in academic laboratories.

The assessment of composition profile will be performed by HPLC – DAD- MS, capillary electrophoresis, fluorimetric methods, chemiluminescent method etc. Silymarin extraction will be accomplished using: ethyl alcohol, methyl alcohol, ethyl acetate, dioxane, water or various mixtures solvent/water. It will be performed the study of the temperature influence and the influence of the extraction technique used: percolation / maceration, Soxhlet, and accelerated extraction based on the use of cavitation phenomena (ultrasound or microwave assisted) on sylimarin extraction yield. The aim is to settled extraction technologies based on the use of non-flammable solvent (or with reduced flammability) and mild working conditions, as technology agreed by trader. The extracts antioxidant capacity and their antitumoral effects will be assessed. The obtained silymarin concentrate will be used by HOFIGAL in phyto-drugs formulation and food supplements.

Sensitive and selective detection of neurotoxic insecticides based on cholinesterase biosensors coupled with solid phase extraction Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-0367 2017 - 2018

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); CP MED LABORATORY SRL (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://www.incdsb.ro/p/COLIN-SP/PED45-2017.htm

Abstract:

This applicative project aims to develop a selective and sensitive analytical method useful for screening of neurotoxic insecticides in environmental samples based on a combination of the specific advantages of acetylcholinesterase biosensors and solid phase extraction (SPE). The biosensors provides the low-cost and easy to use analytical devices while the sample pretreatment with SPE ensures the required sensibility and results reliability due to interference removal from unknown samples. The successful combination of biosensor with SPE is based on optimization of the enzymatic inhibition buffers that contains optimized percentages of various water miscible organic solvents and choice of the appropriate sorbent-elutant for analyte separation. A heterogeneous oxidation of the organophosporothionate insecticides on the SPE column will be tested for improved sensitivity. The consortium combines the vast experience in the field of cholinesterase biosensors and enzymology of National Institute of R&D for Biological Sciences with the know-how of S.C. CP MED Laboratory S.R.L. in solid-phase extraction, reliable analyses for environmental pollution monitoring and method validation.

Multiparametric system for wine monitoring fermentation Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0297 2014 - 2017

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); UNIVERSITATEA BABES BOLYAI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); CEPROCIM S.A. (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://www.incdsb.ro/p/Senswine/PCCA0297-SENSWINE-WEB.htm

Abstract:

The objectives of this project are the development of a complete analytical solution for the wine sector consisting of: (1) a multiparametric flow analysis system based on enzymatic biosensors for monitoring of alcoholic and lactic must fermentations (2) mineralogical and geochemical investigations of soil from vineries and (3) detection of heavy metals from soil and winemaking relevant matrices (grapes, must and wine). The European winemaking sector is facing an intense competition from imports of low-cost wines and is under pressure to improve the products quality. Because the winemaking process is an “art” highly dependent on the producers' experience and skill, fast, cheap and reliable analytical devices and services are vital to obtain an effective control at all stages of the production. The current analysis techniques (FTIR or chromatographic analyses) are long and expensive due to complexity of the sample matrix and too complex to be used in situ by the average winemaker. This multidisciplinary project will develop two enzymatic biosensors for the detection of alcohol (based on oxidases) and respectively lactic acids (based on dehydrogenases). The biosensors may be used independently or integrated in a multiparameter flow analytical system that will quantify the signal using a analog potentiostat. The heavy metal detection is important for two reasons: (1) they are involved in taste and flavor development and (2) to protect the consumers of contaminated products. The soil has a major influence on the grape quality and provides the wine specificity that determine the consumers fidelity for the products obtained from a specific "terroir". The combination of all three analytical systems provides a complete solution adaptable to the specificity of winemaker from various European countries each one with different domestic market and background.

Obtaining new vector types for their phytotherapeutical principles, by means of biotechnological processes and the modeling of the phytotherapeutical principle's release mechanism Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0034 2014 - 2017

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); INSTITUTUL DE CHIMIE FIZICA - ILIE MURGULESCU (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU TEXTILE SI PIELARIE-I.N.C.D.T.P. BUCURESTI SUCURSALA BUCURESTI INSTITUTUL DE CERCETARE PIELARIE - INCALTAMINTE I.C.P.I. (RO); UNIV.DE MEDICINA SI FARMACIE - CAROL DAVILA (RO); CENTRUL DE CERCETARE SI PRELUCRARE A PLANTELOR MEDICINALE PLANTAVOREL SA (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://www.incdsb.ro/p/NEWBIOVECT/index.htm

Abstract:

The goal of the NEWBIOVECT consortium is to develop a new biomimetic biovectors – complex of the multi-membranes and active principles of a phytocomplex obtained from Symphytum officinale, Betonica officinalis, Salvia officinalis and Impatiens L. species concentrated extracts, such as polyphenols (can mimic the effects of cytokines, they have also been involved in the regulation of cell division and expansion), flavones, saponins (anti-inflammatory compounds that mimic the action of steroids), allantoin and polysaccharides (stimulate proliferation of keratinocytes and dermal fibroblasts). The project also aims to release in vitro and in vivo a therapeutic dosage of drug that heals wounds and reduces the inflammatory effects. The new biovectors will be will be kinetically characterized and will be establish of the mechanisms involved in the kinetic processes.

The development of new, advanced systems biovector phytotherapeutic compounds (active ingredients) aim to the skin wounds’ treatment improvement as well as shorter the hospital recovery time. This project focuses also on the superior valorizing of some well-known medicinal herbs (Symphytum officinale and Salvia officinalis), as well as the higher valorizing of Impatiens L. Species and Betonica officinalis which has not yet been introduced in Romania, for research and proceeding by now. Additionally, this project aims to transfer the new knowledge in the economic environment for a more efficient use of medicinal and aromatic plant species, and increase the quality and competitiveness of Romanian’s phyto-pharmaceutic products on domestic and foreign market.

The project results will get a technological impact considering the development of some new, nonconventional processes with high efficiency, in Romania, for obtaining and processing the herbs extracts.

The main objectives of the NEWBIOVECT project are: i) design and synthesis of novel polymeric and composite multi-membrane systems, macroporous and nanoporous membranes for active principles of a phytocomplex obtained from Symphytum officinale, Betonica officinalis, Salvia officinalis and Impatiens L. species concentrated extracts delivery; ii) kinetic studies concerning the active principles in vitro release from the obtained supports, and testing the obtained support for their biocompatibility; iii) accomplishment of the biovectors’ experimental lots for the phytopharmaceutical products with reproducible characteristics and evaluate in vivo therapeutic efficacy.

New molecularly imprinted polymers (MIPs) developed for the sensitive and fast analysis of nitrofuran based antibiotics Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0203 2014 - 2017

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); COSFEL ACTUAL S.R.L. (RO); CP MED LABORATORY SRL (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://www.chimie.unibuc.ro/cercetare/analitica/proiect%20197_2014%20Victor%20David.pdf

Abstract:

The objectives of this project are the synthesis of two molecularly imprinted polymers (MIPs) and their use for the development of two analytical methods for the detection of nitrofuran based drugs/metabolites. The illegal use of nitrofuran based drugs (furazolidone, furaltadone, nitrofurantoin, nitrofurazone, etc.) by the livestock industry as growth promoters leads to the apparition of new dangerous bacteria strains that are resistant to antibiotic and produces dangerous metabolites that remain for a long time in meat. The EU regulations impose the control of biological relevant samples collected from living animals (urine, blood), meat from slaughter houses or traders and prepared foods. The current analysis techniques are long and expensive due to complexity of the sample matrix and low concentration of the analytes. The two MIPs synthesized in this project will be used in two different analytical applications: (1) the development of affinity based sensors and (2) for sample pre-treatment by solid phase extraction (SPE) before chromatographic analysis. The sensors will be used for rapid screening of biological samples and constructed by the immobilization of the MIPs on a transducer and detection using an appropriate detection technique like electrochemical impedance spectroscopy (EIS) or quartz crystal microbalance (QCM). The main advantages of the sample pre-treatment with MIPs in chromatographic analysis are analyte isolation and concentration from complex matrices. Due to the high polarity and low detectability in UV-VIs absorption, the project will be focused also on derivatization of chosen analytes in order to enhance their hydrophobicity and fluorescence labeling. This procedure is carried out after extraction of analytes from samples by means of MIPs. The project is industrial driven and the consortium will take important steps in order to prepare a successful commercialization of the developed MIPs such as: (1) the MIPs development and optimization at laboratory scale will take into account not only the analytical performances, but also the requirements to adapt the synthesis to the industrial mass production, (2) the production of the developed MIPs will be tested at industrial scale level by the SME that will continue the post-project production and (3) the developed MIPs will be thoroughly tested by a laboratory specialized in analytical services in order to evaluate the fulfillment of end-user needs.

Higher Valorisation of Vegetal By-Products and Wastes to Support Sustainable Use of Natural Resources Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0361 2014 - 2017

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://www.incdsb.ro/p/HIVEGRES/index_ro.html

Abstract:

The project Higher Valorisation of Vegetal By-Products and Wastes to Support Sustainable Use of Natural Resources - HIVEGRES- proposes, in general terms, new technologies able to improve the natural resources use (wastes and by-products) and to increase the efficiency of bio-products formulation in terms of process control and formulation technology. The project frames in the general field of Industrial Green Technology aiming to acquire, by safe methods, an enhanced content of high quality natural nutraceutics to be used as supplements and additives in obtaining efficient functional phyto-products.

HIVEGRES project aims to provide industrial innovative solutions (sustained by a strong scientific basis) to cover the industrial activities which are supporting the base for launching new healthy products, conforming to EU regulations and claims, with lower processing and production costs while enhancing the natural resources sustainable use; the project is focusing mainly on the phyto-pharmaceutical products sector.

In the frame of present project the practical addressed issues are:

1) Improved, sustainable use of natural resources by recycling vegetal wastes and by-products, improving the production yields and, consequently saving material from disposal while improving the whole process

2) Introducing new tools to control and monitor the production process and to assess the products quality: nano-sized biosensors array and flow injection analysis (FIA) methods and device

3) To create new opportunities for functional phyto-products diversification and opening new prospects in the market segment for the involved Small and Medium Enterprises (SMEs).

To achieve the main goals of the project within the proposed 24 months project, the following main active principles are envisaged: the most efficient xanthophylls (Lutein and Zeaxanthin), carotenoids and anthocyanins from vegetal by-products, respectively wastes. For each active principle a specific vegetal source will be addressed, accordingly to the existing by-products and wastes and to the expected content of compounds occurring in each source.

Targeted vegetal materials are: by-products resulted from Vaccinium vitis-idaea, (cowberry), Myrtilli fructus and leaves (bilberry), Cynosbati fructus and leaves (rosehip), Hippopheas fructus (sea-buckthorns) and leaves (wild and cultivated in Romania, mainly Northern part) and vegetables wastes resulted from cultivars (all over the country) of tomatoes, carrots and egg plants.

Two important innovative approaches are proposed by HIVEGRES: in the products quality control (raw material and final products) by introducing nano-sized sensors able to provide information which comply to EU claims, and in natural resources processing technology, by proposing an improved extraction technology able to lead to higher extraction yield of active principles.

Integrated system for monitoring and bioremediation of metal/radionuclides - contaminated area Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0608 2014 - 2017

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); CP MED LABORATORY SRL (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU METALE SI RESURSE RADIOACTIVE - I.C.P.M.R.R. BUCURESTI (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://www.incdsb.ro/p/Imonbio/Proiect.html

Abstract:

The core objective of the proposal consists in the development of new integrated system for monitoring and bioremediation of metal/radionuclides from contaminated areas, this meaning the complex approach of the subject from the following points: development of new analytical methods/devices applicable to fast assessment/monitoring of heavy metals and radionuclides from contaminated areas; development of a bio-remediation strategy based on micro-organisms effective against the two classes of contaminants. The project proposes as monitoring devices an array of electrochemical (enzymatic and micro-organism based) biosensors, selective and sensitive for heavy metals and radionuclides. Envisaged heavy metals are copper, lead, mercury, cadmium, zinc, while envisaged radionuclides are uranium, radon, and thorium.

The following subsidiary objectives are envisaged:

 Design of a microorganisms based bio-remediation strategy and development of the experimental model on lab-scale;

 The versatile use of Pseudomonas and Clostridium strains, both in bio-remediation strategy (as biocatalysts) and in biosensors construction (as pollutants bio-recognition element).

 Design, construction and validation of the new analytical device: biosensor platform based on microorganisms and enzymes immobilization.

 Design of a demonstrator model to support the bio-remediation strategy

Sustained dissemination actions (ISI papers, Conferences) and patent request are also planned.

Inovative, multidisciplinary research to investigate the probiotic effects of new lactic acid bacteria strains and consortia Call name: Joint Applied Research Projects - PCCA-2011 call, Type 1
PN-II-PT-PCCA-2011-3.1-0969 2012 - 2016

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL DE BIOLOGIE (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); UNIVERSITATEA DE STIINTE AGRONOMICE SI MEDICINA VETERINARA (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://genetmicroproiecte.blogspot.ro/search/label/PROLAB%2077

Abstract:

The emergence of bacterial resistance and multiresistance to antibiotics represent a major public health problem in Romania and also in the entire world. In Romania, there is an increasing trend of the incidence of infections with bacterial strains highly resistant to antibiotics, whose prognosis is often worsened by the producing of bacterial biofilms on the tissues and biomaterials used in medicine. In our country, the problem of development of some alternative strategies for the prevention and treatment of the infections with multiresistant and biofilm forming bacteria is less studied, despite their great application potential in biomedicine and their utility in the recovery of the balanced or eubiosis state of the normal microbiota, after the negative impact of the antibiotics on bacterial communities. One of the most viable alternatives is represented by probiotics products which by administration in adequate amounts confer a health benefit on human organism. For the best use of the probiotic microorganisms, the mechanisms by which they work should be better understood. The selection of an appropriate probiotic strain for its inclusion in a probiotic preparation should be made on the basis of its capacity to induce an improved host response without modification of the intestinal homeostasis.

The main goal of this project imply complex studies of probiotic strains (lactic acid bacteria newly isolated) and multispecific consortium in order to improve the experimental protocol for the optimal selection of human use probiotics, based on their anti-infective and immunomodulatory activities.

New BImetallic nanoparticles with applications in water CLEANing of chlorinated compounds and BIOSensors Call name: Joint Applied Research Projects - PCCA-2011 call, Type 1
PN-II-PT-PCCA-2011-3.1-0054 2012 - 2016

Role in this project: Key expert

Coordinating institution: INSTITUTUL DE CHIMIE FIZICA - ILIE MURGULESCU

Project partners: INSTITUTUL DE CHIMIE FIZICA - ILIE MURGULESCU (RO); INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://www.icf.ro/pr_2011/Project_Bicleanbios.ppt

Abstract:

The project proposes the use eco-friendly bimetallic nanoparticles with controlled composition, morphology, structure and chemical state in catalytic and photocatalytic abatement of toxic chlorinated compounds in water via dehydrochlorination reaction. The fine tuning of nanoparticle structure prepared by polyol method is expected to bring crucial improvements by increasing the catalytic activity and resistance against poisoning compared to existing materials. A strategy for improving the catalytic performances is proposed by using ordered mesostructured Al2O3 as supporting material which will be synthesized by combined microemulsion-hydrothermal techniques. In addition, the photocatalytic hydrodechlorination reaction over bimetallic nanoparticles assembled in a controlled manner with TiO2 will be investigated for the first time. The materials will be characterized by a bunch of physico-chemical techniques (XRD, XPS, fractal analysis, gas titration TPO, TPR, Cyclic Voltammetry). Also they will be tested for cytotoxicity and genotoxicity.

Another important application envisaged is to develop new highly sensitive and stable biosensors based on bimetallic nanoparticles. The immobilized biorecognition element(s) on a modified working electrode with bimetallic NPs will be evaluated to identify the most promising systems for biosensor construction.

The targeted technical goal is to develop a laboratory technology for catalytic and photocatalytic degradation of chlorinated compounds in polluted waters. Sensitive and stable biosensors devoted to TCE assessment and based on bimetallic nanoparticles use are also planned to be developed at prototype level.

In order to achieve these ambitious objectives, in the three years proposed, a well-balanced and multidisciplinary consortium of three partners has been created. All the participant members have a well-established reputation in catalysis, material science, bio-chemistry and physico-chemical characterization techniqu

Exploiting the antioxidant capacity of black grapes for producing wines with high authentic quality Call name: Joint Applied Research Projects - PCCA-2011 call, Type 1
PN-II-PT-PCCA-2011-3.1-1809 2012 - 2016

Role in this project: Key expert

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO); INSTITUTUL DE CERCETARE DEZVOLTARE PENTRU VITICULTURA SI VINIFICATIE (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://www.biodyn.ro/PROJECTS/ANTIOXWIN/antioxwin.html

Abstract:

The Project aims to develop tools for obtaining wines with consistently high quality attributes and propose ways to recognize their unique characteristics, to affirm and protect their identity. More specifically, we target selected red wines with Dealu Mare Controlled Origin Denomination and we will use the antioxidant potential (phenolic profile and total antioxidant capacity) as a means to affirm and verify their quality. We will develop an optimised technological process whose monitoring will include verification of the total antioxidant capacity of intermediary samples using an electrochemical sensor(i); a detailed profiling including, in addition to typical parameters in the wine sheet, a thorough characterisation of the phenolic composition, anthocyanin structure and total antioxidant capacity, all that being synthesized in a database(ii) and novel tools for wine classification and assertion of their authenticity, respectively an "e-tongue" device and a chemometrics model that will exploit the information in the "antioxidant" database to define the "antioxidant fingerprint" of wines. The synergy between the existing infrastructure and scientific capabilities of the partners will allow a complex, multidisciplinary evaluation of wine quality and generation of high quality novel technology, sensors and information representing a significant advancement of the state-of-the art. A particular strength of the project relies on maximizing the extraction of relevant information and practical application of the generated data. The projected end results in ANTIOXWIN (optimised technology, total antioxidant capacity sensor, antioxidant database, e-tongue and antioxidant fingerprint) will offer new tools to wine-makers, quality control labs, regulators in the field of quality wines with controlled origin etc and will raise consumer awareness with regards to the quality of Romanian wines.

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List of research grants as project coordinator	Download (418.55 kb) 08/04/2016
List of research grants as partner team leader	Download (459.23 kb) 08/04/2016
List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
R&D activities in enterprises
Peer-review activity for international programs/projects	Download (5.19 kb) 08/04/2016