BrainMap

Mrs. Daniela Marin

Dr

- INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA - IBNA BALOTESTI

Researcher

Curriculum Vitae (03/06/2020)

Expertise & keywords

Investigation of pre- and probiotics as modulators of microbiota and intestinal processes in Inflammatory Bowel Diseases using pig as animal model Call name: Projects for Young Research Teams - RUTE -2014 call
PN-II-RU-TE-2014-4-1287 2015 - 2017

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA - IBNA BALOTESTI

Project partners: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA - IBNA BALOTESTI (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA - IBNA BALOTESTI (RO)

Project website: http://www.ibna.ro/en/activitate/proiecte/te302_en.php

Abstract:

The concept of human health covers not only the human diseases, but also the critical relationship between wellbeing and disease, as well as its relevance to public health. Besides the diseases with greater impact on public health, besides the most prevalent diseases is inflammatory bowel disease (IBD), chronic and remitting disorder of the gastrointestinal tract. There is an increasing interest for use of natural products as active compounds which can prevent/attenuate the inflammation in IBD. Besides the promising ways to counteract the intestinal inflammation is the use of probiotics (bacteria and yeast) and of ingredients rich in bioactive compounds as polyphenols. For these promising active compounds are needed more in-depth studies to elucidate their impact on the reduction the prevalence of IBD. For this purpose, we assembled a multidisciplinary team to delineate the impact of prebiotics (polyphenols from grape pomace) and probiotics (Lactobacili sp.) in counteracting the inflammatory processes and in modulating the microbiota, using pig as animal model for IBD. The effect of grape pomace polyphenols and of probiotics will be investigated in in vitro and in vivo studies. We aim to see in which way the polyphenol compounds of GP in combination with several Lactobacilli sp. could stimulate the mucosal immune response and increase therefore the defence and resistance potential of the animal.

Impact of feed co-contamination and mitigating solutions to increase feed safety, animal health and food quality. Call name: Joint Applied Research Projects - PCCA-2011 call, Type 1
PN-II-PT-PCCA-2011-3.1-0616 2012 - 2016

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA - IBNA BALOTESTI

Project partners: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA - IBNA BALOTESTI (RO); INSTITUTUL ONCOLOGIC PROF.DR.I.CHIRICUTA CLUJ-NAPOCA (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE IN DOMENIUL PATOLOGIEI SI STIINTELOR BIOMEDICALE "VICTOR BABES" RA (RO); ASOCIATIA CENTRU DE CERCETARE STIINTIFICA IN DOMENIUL BIOCHIMIEI APLICATE SI BIOTEHNOLOGIEI (BIOTEHNOL) (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA - IBNA BALOTESTI (RO)

Project website: http://www.ibna.ro/activitate/proiecte/PN2-parteneriate.php

Abstract:

The extent and impact of the situations when animals are exposed to more than one contaminant remain to be analysed and quantified and this is the major objective of this research project. That is why, the present project will develop a comprehensive approach to reach a better understanding of the interaction between different contaminants of feed and food: co-contaminants and propose mitigating solutions in case of feed co-contaminations. One of the most often reported co-contamination in the field includes microorganisms (Echerichia coli, Salmonella etc, the main food borne human pathogens originated from the animal gut) and natural toxins such as mycotoxins, which are the most frequent natural contaminants of cereals. For many contaminants of the agro-food chain there is no regulations/legal limits of tolerance issued by the European Commission, the National Ministries of Agriculture or by the National Sanitary Veterinary Agencies. The elaboration of some regulations and its enactment is imperiously necessary. More recently, EFSA (EFSA Journal, 2010) recommends conducting in-depth studies to document that the doses accepted as maximal limits have no genotoxic effects. Using new tools of genomics and proteomics (PCR microarrays, real time PCR, western blot, xMAP array), the present project will give a better insight in the mechanisms of action of co-contamination and based on the data supplied by these new tools it want to determine the maximal tolerance levels (norms) for some contaminants commonly present in the growing pigs (10-30 kg) diet. The project also, propose mitigating solutions in case of feed co-contaminations In this regard a probiotic bacterial product is currently under development in IBNA laboratory which has proved healthy benefits and excellent efficacy in reducing the gastrointestinal infections in pig as well as in other farm animals either in experimental trails or in commercial farms. The project focuses on the pig. The pig is a great cons

Safety and tolerability of calcium and iron-enriched oats containing gluten-free diet Call name: Joint Applied Research Projects - PCCA-2011 call, Type 2
PN-II-PT-PCCA-2011-3.2-0695 2012 - 2016

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIORESURSE ALIMENTARE - IBA BUCURESTI

Project partners: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIORESURSE ALIMENTARE - IBA BUCURESTI (RO); INSTITUTUL NATIONAL PENTRU SANATATEA MAMEI SI COPILULUI "ALESSANDRESCU-RUSESCU" BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA - IBNA BALOTESTI (RO); PHARMACORP INNOVATION S.R.L. (RO); FARINSAN SA (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA - IBNA BALOTESTI (RO)

Project website: http://safeoatdiet.bioresurse.ro

Abstract:

Gluten-sensitivity vulnerable population is becoming an important sector in the food industry marketplace. The food industry therefore can no longer afford to ignore the particular needs, likes and dislikes of this group. The aim of the project is to understand the role of nutrition in sustaining health and preventing disease. The main project objective is to obtain calcium and iron enriched oat-based bakery products using organic sources (calcium and iron-rich yeasts). Three research directions are proposed: (i) studies on the effect of oat addition in conventional baked products (rheology, processing, sensory attributes, texture, consumer preference); (ii) design and analyzes of oat-based gluten-free products and (iii) production and analyzes of calcium and iron enriched yeasts biomasses in two forms: active (fresh/compressed) and inactive (dried/powder) which will be further introduced in baked products (both conventional and gluten-free). Pharmacological studies are foreseen for assessing the capacity of enriched yeasts to modify the integrity of the epithelial monolayer in Caco-2 cells cultivated in single layer on filters. The shown tolerability of the designed new oat-based products for the gluten-intolerant population studied here in an evidence based manner, will be one milestone for product export. The designed new dietary products are foreseen to have a broader market within EU member states than only in Romania. New recipes and technology, technology know-how obtained in collaboration with industry, especially calcium and iron enrichment of products by using yeast strains, production of calcium and iron-rich yeast biomasses, both inactive (dried) and active (compressed) and designing of new oat-based products will be protected by patents. Thus intellectual property rights are staying within Romania and will be of future benefit for Romanian food technology industry and may also be the initiator of new companies, spin offs from research institutes.

Valorization of wine industry waste as a source of antioxidants for an enriched animal diet Call name: Projects for Young Research Teams - TE-2012 call
PN-II-RU-TE-2012-3-0048 2013 - 2016

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA - IBNA BALOTESTI

Project partners: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA - IBNA BALOTESTI (RO)

Affiliation:

Project website: http://www.ibna.ro/activitate/proiecte/te31.php si http://www.ibna.ro/en/activitate/proiecte/te31.php

Abstract:

In recent years, a strong request to the agro-food industries for cleaner production processes has been noticed, and so the efficient and profitable recycling of wastes from agro-food industries have received rising attention. Concerning this recycling process, one of the most promising opportunities is given by the recovery of biologically active extracts or compounds as a source of high-added value products potentially useful as beneficial functional food and feed constituents, food flavors and antioxidants, cosmetics, drugs or drug adjuvants. Wine by-products represent sources of antioxidants that have been relatively unexploited to date in Romania, but they are subjects of increased industrial interest. No evidence is available on the potential antioxidant properties of grape pomace (GP) when added in pig diets in Romania so this would be the first study involving in vivo experiments correlated with in vitro cell culture. Studies investigating the use of this residue may lead to significant economic gains and to prevent or decrease environmental problems caused by GP accumulation. The GP will be analyzed and tested through an extended and complex approach involving new in vitro and in vivo concepts along with the classical antioxidant activity in view of elaborating new and cheap sources of feed. The results of the applied science as aimed by this project will open new dimensions of the biochemistry of antioxidant polyphenols through the involvement of o-quinones.

Studying the toxicity of zearalenone, natural contaminant of pig feed, using new tools of nanotehnology; contribution to public policy on feed safety Call name: Exploratory Research Projects - PCE-2011 call
PN-II-ID-PCE-2011-3-0339 2011 - 2016

Role in this project: Key expert

Coordinating institution: Institutul National de Cercetare-Dezvoltare pentru Biologie si Nutritie Animala - IBNA Balotesti

Project partners: Institutul National de Cercetare-Dezvoltare pentru Biologie si Nutritie Animala - IBNA Balotesti (RO)

Affiliation:

Project website: http://www.ibna.ro/activitate/proiecte/PN2-idei.php

Abstract:

Zearalenone (ZEA), an estrogenic mycotoxin produced by several fungi of Fusarium genra could contaminate food and feed being a risk factor for both a public health and agronomic perspective. Most studies revealed the specific toxic effect of this toxin on the reproductive apparatus in laboratory and farm animals. In addition, ZEA has been shown to be immunotoxic in low concentrations. The pig, a great cereal consumer, is considered by EFSA the most sensitive species to ZEA action. The economic losses caused by feed contamination with ZEA can be important for this species, particularly during the weaning period, which is much more difficult for the pigs than for other animal species. Although the studies on ZEA intensified lately, the mechanisms of its toxicity are yet to be ascertained. Using new tools of genomics and proteomics (PCR microarrays, real time PCR, western blot), the aim of the present project is to obtain a better insight in the mechanisms of action of ZEA and the changes produced by this toxin in gene expression of pig immune response and MAPK signalling pathway driving activation of genes responsible for the immune competence. The project will develop a microarray for pig immune response which has not been performed until now only for deoxynivalenol in mice and for aflatoxin B1 in broilers. Using data generated by this microarray the project will contribute to the determination of the maximal tolerance levels (regulation) for ZEA in the growing pigs.

Role of ochratoxin A in triggering the Balkanic endemic nephropathy using pig as animal model Call name: Projects for Young Research Teams - TE-2012 call
PN-II-RU-TE-2012-3-0086 2013 - 2016

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA - IBNA BALOTESTI

Project partners: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA - IBNA BALOTESTI (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA - IBNA BALOTESTI (RO)

Project website: http://www.ibna.ro/activitate/proiecte/te4.php

Abstract:

The project investigates the involvement of ochratoxin A in the balcanic endemin nefropathy.It will yield data on the effects of OTA at the cellular and molecular level, regarding cell growth and division, oxidative stress and inflammatory response, which will be an original, pioneering approach of this topic in Romania. The results will form the scientific basis for the changes induced by OTA in the kidney and within the entire organism; they may be correlated with the clinical observations of the Balkan nephropathy, proving or not the involvement of OTA in this disease. Besides the national benefits, the risk analyses conducted during the project will contribute to the consolidation (completion) of the European database on the cellular and molecular toxic effects of OTA in animals; this will allow the consolidation of the EU food safety legislation and the issue of new EU regulations on the limits of tolerance of OTA concentrations in animal feeding, knowing that so far there is only a EU recommendation (CE Recommendation 576/ 2006) regarding the limits of tolerance of OTA concentrations in forages. The regulation of OTA concentration will eliminate from consumption the foods and feeds with OTA concentrations higher than the highest concentration allowed. At the same time, the increased food safety and security will improve the health state of the animals and, implicitly, of the human population.

FILE DESCRIPTION	DOCUMENT
List of research grants as project coordinator
List of research grants as partner team leader
List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
R&D activities in enterprises
Peer-review activity for international programs/projects