BrainMap

Coralia Bleotu

- INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU"

Other affiliations

- UNIVERSITATEA BUCURESTI (Romania)

Researcher | Scientific reviewer

Web of Science ResearcherID: Web of Science ResearcherID  B-5976-2011

Expertise & keywords

Wastewater-based epidemiology concept as an early warning system for SARS-CoV-2 trend and its circulating variants in a defined catchment Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-4131 2022 - 2024

Role in this project:

Coordinating institution: INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU"

Project partners: INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO); INSTITUTUL NATIONAL DE CERCETARE -DEZVOLTARE PENTRU ECOLOGIE INDUSTRIALA - ECOIND (RO)

Affiliation:

Project website: https://virology.ro/grant/wastewater-based-epidemiology-concept-as-an-early-warning-system-for-sars-cov-2-trend-and-its-circulating-variants-in-a-defined-catchment/

Abstract:

Covid-19 disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in Wuhan, China in December 2019 and gave rise to a worldwide health emergency after only 2 months. Currently, more than 226 million cases have been confirmed and over 4.6 million deaths worldwide. In Romania, the numbers are raising with over 1.1 million confirmed cases and more than 35k deaths. The well-known lineages, B.1.1.7 (Alpha variant), B.1.351 (Beta variant), P.1 (Gamma variant) and B.1.617.2 (Delta variant) assigned as variants of concern (VOCs), show an increased transmissibility and higher infectivity. Lately, it has been observed that the VOCs exhibit an increased resistance towards the vaccines and therapies, especially the Delta variant. Considering the ascending number of infections with SARS-CoV-2 variants and the necessary time for the symptoms onset in infected individuals, a delay of the real active cases reported per day can be observed. Wastewater based epidemiology (WBE) proved to be a powerful tool for the early detection of SARS-CoV-2 concentrations in a certain catchment and to provide a quick snapshot about the circulating variants before they are seen in clinical cases. The scope of the project is to demonstrate that the early detection of an increasing trend in total SARS-CoV-2 concentration and assessment of its circulating variants in wastewater represent the key factor in the pandemic containment. Furthermore, the present proposal aims to demonstrate the infectivity of SARS-CoV-2 in untreated wastewater samples from Bucharest, which is of paramount importance for the public health. Noteworthy, the study aims to support the collective efforts of the EU Sewage Sentinel System by implementing a wastewater based surveillance approach in Romania as recommended by the European Commission. EU strongly encourages the member states to implement the wastewater surveillance system for SARS-CoV-2 and its variants before October 1st 2021.

Mechanisms and biomarkers of response and resistance to current targeted therapies in gastric cancer Call name: P 4 - Proiecte Complexe de Cercetare de Frontieră
PN-III-P4-ID-PCCF-2016-0158 2018 - 2022

Role in this project:

Coordinating institution: INSTITUTUL CLINIC FUNDENI

Project partners: INSTITUTUL CLINIC FUNDENI (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE CRAIOVA (RO); INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO)

Affiliation: INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO)

Project website: https://cemt.ro/proiect/therres/

Abstract:

Gastric cancer (GC) is a heterogeneous disease with almost one million new cases occurring annually worldwide. Recent developments provide the unprecedented opportunity to make substantial progress in GC therapy. Targeted therapies have revolutionized the therapy of GC, but the benefits remain limited to a few months of increased survival.

The challenge is now how to best stratify patients for therapy, and to identify ‘druggable’ primary and acquired resistance.

Limited studies have been performed to evaluate biomarkers for predicting response to anti-HER2, anti-MET, anti-VEGFR2 therapy in GC, and immune checkpoints that contribute to tumor resistance. Moreover, combining targeted strategy with immune checkpoint inhibition – the most exciting and active area of research currently because of durable responses seen in melanoma and lung cancer – are among the new frontiers of research in the cancer treatment and could represent a quantum leap in the therapy of GC.

The project specific objectives are:

Objective 1: To explore potential biomarkers and targets for therapy in GC subtypes defined based on contemporary disease classifications

Objective 2: To examine biomarkers of response and resistance to standard anti-HER2, anti-MET, and anti-VEGF therapy in GC

Objective 3: Characterization of the particular features of GC vascular stroma, including the study of the potential role of immune checkpoints in GC subtypes

As a key output, the project will generate decision making tools for treatment and management of GC patients in a personalized approach. The results of the present project will help improve the performance, quality and international visibility of Romanian scientific results in oncological field.

Development of anticancer nanoparticulate systems based on novel metal complexes Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-5143 2020 - 2022

Role in this project:

Coordinating institution: INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU"

Project partners: INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE "CAROL DAVILA" (RO)

Affiliation: INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO)

Project website: https://www.virology.ro/ro/cercetare/proiecte/proiect-ped-383-2020

Abstract:

A new generation of efficient metal-based drugs needs to be developed in order to overcome the biological, biopharmaceutical and biomedical drawbacks of standard chemotherapy. Use of metallo-drugs in cancer treatment has been hampered by an indaquate pharmacokinetic profile that limited the access to the biological target. This has led to the development of novel technologies based on nanostructured materials, acting as vectors for metallodrug delivery or simply as protectors of active species of the complexes for amplifying their activities and reducing their degradation. The aim of the project is to develop and validate new liposomal systems for transport and delivery of 2 promising anticancer agents previously studied by our research group. Nanoparticulate drug carrier systems will improve the therapeutic effectiveness and safety profile of these novel anticancer agents. The efficiency of these nanoformulations will be evaluated in terms of stability, drug entrapment efficiency, release behaviour, cell uptake, cytotoxicity, farmacotoxicology and pharmacokinetics. The project specific objectives are: 1) Development of liposomal systems validated at the laboratory level with novel anticancer agents; 2) Estimation the in vitro biological activity of the liposomal systems on various cell lines, including drug resistant cell lines, identifying biomarkers involved in modulating their biological activity; 3) Estimating the pharmaco-toxicological and pharmacokinetic profile of liposomal systems. Nanoparticles provide an enhanced bioavailability, in vivo stability, intestinal permeability, solubility, sustained and targeted delivery, therapeutic effectiveness of the anticancer drugs. The proposed project is a complex, interdisciplinary study related to biomedical international research, that aims to intends to transform modern methods, worldwide used in pharmacological, immunological and molecular biology studies, in instruments used for the translation to oncology.

Evaluation of the exploatation potential Potential of Porous Materials in the tREatment of Microbiota-related dISeasEs - PREMISE Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-4018 2020 - 2022

Role in this project:

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO); INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO)

Affiliation: INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO)

Project website: https://www.micronanotech.ro/evaluarea-potentialului-de-exploatare-a-materialelor-poroase-in-tratarea-disbiozelor-microbiotei/

Abstract:

The scope of the current project proposal is to develop novel micro- and mesoporous materials (MMMs) using recent synthesis methods and to further functionalize these materials for developing better performances in order to be used as drug delivery systems (DDS), especially for microbiota-related diseases. These drug delivery systems are developed for oral administration and are expected to be protected within the stomach and maintain their activity until reach the desired site. Even if these drug delivery platforms are extensively exploited in the literature for several types of applications as DDS (hosting anticancer, anti-infectious or analgesic agents), the field of microbiota is only marginally treated. Nowadays, especially in the case of children, the awareness about the importance of the microbiota is well known, therefore after an antibiotic treatment, the prescription of probiotics is frequently needed. In the project’s boundaries, natural substances (polyphenols and vitamins) used as biological active agents will be loaded into the porous platforms, in order to assure a positive feedback to the microbiota due to the antioxidant, antimicrobial, anti-inflammatory and anticancer activities. In recent years, great emphasis has been placed on the development of micro and mesoporous materials loaded with biological active agents, but few research papers discuss the impact of functionalized MMMs on microbiota. In the current project, the main goal is to develop innovative MMMs with enhanced pore system able to host and deliver biological active agents (vitamins and polyphenols are expected to be used) for the treatment of the microbiota-related diseases. because most polyphenols have low solubility, two innovative delivery systems will be developed in order to assure triggering delivery / smart delivery.

Selection and dissemination of antibiotic resistance genes from wastewater treatment plants into the aquatic environment and clinical reservoirs Call name: P 4 - Proiecte Complexe de Cercetare de Frontieră
PN-III-P4-ID-PCCF-2016-0114 2018 - 2022

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE -DEZVOLTARE PENTRU ECOLOGIE INDUSTRIALA - ECOIND (RO); INSTITUTUL NATIONAL DE BOLI INFECTIOASE ''PROF.DR.MATEI BALS'' (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE MEDICO-MILITARA „CANTACUZINO” (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://bios.unibuc.ro/Proiecte/pn-iii-p4-id-pccf-2016-0114.html

Abstract:

The RADAR project aims to assess the prevalence and dissemination of antibiotic resistance (AR) from urban, clinical and industrial wastewater into the aquatic environment via wastewater treatment plants (WWTPs). Moreover, RADAR will give information on the environmental and clinical resistome, identifying the possible mechanisms of AR emergence and spread.

Scientific objectives: i) investigation of WTTPs resistome to establish the prevalence of AR bacteria (ARB)/genes (ARGs) in the environmental samples from upstream-WWTP-downstream transects; ii) evaluation of selected antibiotics (beta-lactams, fluoroquinolones and macrolides) occurrence in both WWTP’ influent and effluent; iii) comparative analysis of geographically and time related ARB/ARGs from wastewaters and clinical sources; iv) assessing and prediction how the presence/absence of ARGs and their relative abundance depending on a class of geographical, hydrological, physico-chemical and microbiological factors.

Methodology: The urban, clinical, farming and industrial wastewater will be monitored by physico-chemical, microbiological methods and metagenomics throughout various steps, i.e.: 1000 m upstream river, influent, different treatment steps inside WWTP, effluent and 200 m downstream river. Wild fish from the receiving river after discharge of WWTP effluent will be also analyzed. The following analyses will be performed: i) LC/MS and HPLC techniques to monitor the levels of selected antibiotics; ii) isolation and identification of ARB belonging to ESKAPE species; iii) evaluation of metagenomic resistome by bioinformatic data processing; iv) environmental and clinical ARGs and plasmids sequencing; v) analyzing the potential of ARGs for transferability and environmental/clinical risk by mapping the insertion loci of transposable elements and the ARGs potential to mobilize the resistance genes in a bioreactor model; vi) elaboration of a prediction model for the occurrence of ARB/ARGs

INNOVATIVE BIONANOMATERIALS FOR TREATMENT AND DIAGNOSIS Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0629 2018 - 2021

Role in this project:

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE "CAROL DAVILA"

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE "CAROL DAVILA" (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE TARGU MURES (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE "CAROL DAVILA" (RO)

Project website: https://umfcd.ro/cercetare-si-dezvoltare/proiecte/proiecte-nationale/bionanomateriale-inovative-pentru-tratament-si-diagnostic/

Abstract:

In the field of curing different maladies the current medicine make special efforts for the control of factors that favor their appearance together with pathogen agent surveillance. The development of modern medicine is strongly influenced by technology and in this view together with the development of tissue engineering it was tried the creation of a replacement for human bony tissue. In the preset project the research efforts from the forth component projects are implied in:1- antimicrobian nanomaterials; 2- biomaterials for tissue regeneration and for diagnosis, prevention and treatment of osteoporosis; 3- intelligent nanocomposites for diagnosis and treatment bony cancer; 4- nanostructured probes dedicated to diagnosis. The research concerning the obtaining of new intelligent bioceramics with antiseptic properties dedicated to bony tissue regeneration together with their physical-chemical characterization implies the elaboration of protocols for obtaining, analysis and prophylaxis at laboratory animals tests level. Biomaterials application in the field of tissue regeneration for osteoporosis case will bring experimental data related to structural and morphological characterization of nanomaterials together with information regarding biocompatibility and cytotoxicity. These data that will lead to composites optimization as a function of histological character common to laboratory animals. The study of intelligent nanocomposites will open a direction related to their action in bony cancer case. The development of photoluminescent biocompatible ceramic nanomaterials is dedicated to bony screening and further more will their functionalization with anti-inflammatory materials in order to evaluate their in vivo action and their possible using as biological probes. The statistics for the osteoporosis appearance in different population decades indicate their maximum in the (41-60) age limit, and in this view, the studies presented in this project are of general import

Advanced biodegradable materials based on MgB2 resistant to microbial colonization Call name: P 3 - SP 3.2 - Proiecte ERA.NET - COFUND
COFUND-M-ERA.NET II-BIOMB 2017 - 2021

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA

Project partners: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://infim.ro/project/biomb/

Abstract:

The innovation of this project consists in the evaluation for the first time of the MgB2 potential for biomedical applications, although it is currently produced for superconductivity devices. Expectations are to generate new MgB2-based composite multifunctional biomaterials with antimicrobial/antifouling properties, and an increased biocompatibility at interfaces between the material and the biological media.

The MgB2 powders, coatings and bulks could be used in biodegradable implants or drug delivery systems, handles and surgical tools, catheters, wound dressings and so on. The mechanical and physico-chemical properties of the proposed materials will be investigated by a comprehensive approach, and bioevaluation will include in vitro and in vivo assays. The MgB2 materials are viewed as solutions for space and time- scale controlled variation of the functional properties required for different bio-applications.

SOL-2020-2 4. Development of innovative solutions for the protection of personnel (professionally exposed) and the population against SARS-CoV-2 virus contamination Call name: P 2 - SP 2.1 - Soluţii - 2020 - 2
PN-III-P2-2.1-SOL-2020-2-0208 2020 - 2020

Role in this project:

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE "CAROL DAVILA"

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE "CAROL DAVILA" (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); SPITALUL UNIVERSITAR DE URGENTA BUCURESTI (RO); STIMPEX S.A. (RO); UNITATEA MILITARA 02433 BUCURESTI (RO)

Affiliation:

Project website: https://umfcd.ro/cercetare-si-dezvoltare/proiecte/proiecte-nationale/dezvoltarea-de-solutii-inovative-pentru-protectia-personalului-expus-profesional-si-a-populatiei-impotriva-contaminarii-cu-virusul-sars-cov-2/

Abstract:

The project is realized by a large consortium consisting of 3 research units, a university hospital and an economic production unit and offers solutions related to the following general objectives:

Reducing the risk of SARS-CoV2 virus contamination of professionally exposed personnel (doctors) and the population.

Ensuring an appropriate level of protection for professionally exposed personnel and the population in the context of the COVID-19 pandemic

These objectives are fulfilled by the development of a technology and an afferent modular production installation, for obtaining equipment of microbiological protection of the medical staff and / or of the general population. The project aims to capitalize on a patent application and a prototype installation that already exists at the economic agent to develop a technology capable of impregnating protective equipment made of textile fabrics with a content of at least 40% natural fibers with nanoparticles of metal oxides in order to obtain of antimicrobial, antifungal and antiviral properties (up to TRL-8). The project aims to homologate a protective equipment, a technology to obtain it and a modular installation that can be used in any hospital in Romania. The technology can be used both to obtain antiviral protection equipment (used by medical staff and civilians) but also to impregnate patients' costumes, hospital bed linen and will help both reduce the transmission of SARS-Cov2 virus and reduce cases of nosocomial infections.

Regression-based characterization of mechanisms involving CEACAM-1 in melanoma – an innovative approach for optimizing the management of patients with thin melanomas Call name: P 4 - Proiecte de Cercetare Exploratorie
PN-III-P4-ID-PCE-2016-0641 2017 - 2019

Role in this project:

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE "CAROL DAVILA"

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE "CAROL DAVILA" (RO)

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE "CAROL DAVILA" (RO)

Project website: https://umfcd.ro/en/research-and-development/national-projects/ceacammel/

Abstract:

Melanoma has overall rising incidence with increased incidence in young patients and high mortality rate in metastatic stage. The extreme variation in natural and therapeutic evolution and unexplained mortality of thin melanomas classified in the same TMN stage highlight the need of new biomarkers and ultrastaging.

The project brings in focus carcinoembyonic antigen cell adhesion molecule 1 (CEACAM1), key molecule involved in tumorigenesis, mainly in epithelial-mesenchymal transition (being responsible for gaining invasiveness and metastatic capacity) and immune escape (by inhibiting effector functions of tumor infiltrating lymphocytes). To decipher CEACAM1 role in modulating aggressive thin melanoma behavior, we chose to study it for the first time in regressing melanoma (RM) vs non-regressing melanoma (nRM), regression phenomenon being used as a valuable model for understanding antitumor immunity.

We will assess CEACAM1 isoforms expression using IHC, RT-PCR and WB in thin RM&nRM melanomas and ELISA for serologic tests. Retrospective studies [tissue microarrays from archived tumor tissue of 90 cases of RM, 90 nRM, 30 halo nevi and 30 common nevi (as benign control)] and prospective studies (plasma, frozen and paraffin-embedded tumor tissue from 30 cases of thin RM, 30 thin nRM, 10 halo nevi and 30 common nevi) will be performed. We assembled a multidisciplinary team including senior and junior researchers under the supervision of a highly experienced researcher with extensive expertise in multiple enzymatic and fluorescent IHC and complex morphometric analysis. We will analyze the correlation between CEACAM1 deregulation and prognosis, intratumor inflammatory infiltrate and consecrated progression-related tissue markers.

This approach will clarify the mechanisms through which CEACAM1 isoforms are involved in progression and regression of thin melanomas, as a starting point for new risk stratification of early melanomas and a better management of these patients.

Sustainable materials based on zinc oxide for antimicrobial applications Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-0240 2017 - 2018

Role in this project:

Coordinating institution: INSTITUTUL DE CHIMIE FIZICA - ILIE MURGULESCU

Project partners: INSTITUTUL DE CHIMIE FIZICA - ILIE MURGULESCU (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.icf.ro/pr_2017/Biosusmat_2018.pptx

Abstract:

The goal of the present project represents the development of new inorganic biocidal agents toward prokaryotic and eukaryotic microbial strains, in planktonic and biofilm adherent state. Its general objective is the optimizing of the design for zinc oxide based nanomaterials, using eco-friendly synthesis in order to reach an effective antimicrobial activity, and the component/subsystem validation in laboratory environment. The obtained ZnO crystallites lies in the nano range. In these context the specific objectives of the project are:

1. selection of the synthesis procedure in terms of antimicrobial and cytotoxic activity;

2. for the selected synthesis procedure, the optimization of the experimental parameters in terms of antimicrobial and cytotoxic impact;

3. validation in laboratory environment of the antimicrobial and cytotoxyc activities;

4. validation in laboratory environment of the synthetic procedure;

5. dissemination and intelectual property protection.

This project aims to develop an inorganic antimicrobial material from technology maturity TRL 3 to TRL 4. The technology maturity TRL 3 of the already undertaken research is shown by: (i) the concept of ZnO nanomaterials with antimicrobial properties obtained via eco-friendly methods is proved experimentally and its efficiency is verified, (ii) each component of the assembly, namely ZnO as inorganic material, the eco-friendly synthesis of ZnO, and the antimicrobial activity of the resulting materials were tested. The final parameter of interest is the antimicrobial activity of these ZnO materials. According to the proposed objectives, the implementation of the project will lead to the integration of the three components in order to establish the functionality of the assembly, the antimicrobial tests being carried out in suitable conditions in accordance with widely approved methods.

Composite hydrogels based on inorganic nanoparticles and collagen with prolonged antimicrobial activity for the prevention of wound infections Call name: P 2 - SP 2.1 - Proiect de transfer la operatorul economic
PN-III-P2-2.1-PTE-2016-0177 2016 - 2018

Role in this project:

Coordinating institution: SANIMED INTERNATIONAL IMPEX S.R.L.

Project partners: SANIMED INTERNATIONAL IMPEX S.R.L. (RO); UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO)

Affiliation: INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO)

Project website: http://nanocolagel.sanimed.ro/

Abstract:

Chronic wounds represent a good niche for biofilm development because the impaired immune response promotes infection susceptibility whilst necrotic tissue and debris favor bacterial attachment. Collagen hydrogels represent one of the most efficient treatments in case of both chronic and acute wounds due to their ability to maintain optimal humidity and aeration parameters. Currently, at a national level there is no production of collagen hydrogels aimed for the treatment of chronic wounds, hence these types of products are being imported. In this context, the present project proposal aims to design and obtain new types of multifunctional collagen hydrogels harboring antimicrobial properties in order to favor the healing process of chronic wounds. As a novelty element in comparison to products currently available on the international market, we will design and produce collagen hydrogels containing nanoparticles. Thus, Sanimed International Impex S.R.L will develop a simple and rapid technology to obtain hydrogels functionalized with metalic and oxidic nanoparticles (collagen hydrogels with Ag nanoparticles, collagen hydrogels with ZnO hydrogels and collagen hydrogels with SiO2@ZnO nanoparticles). The novel hydrogels will be tested for their antimicrobial and antibiofilm properties using in vitro and in vivo methods and also for the host response after hydrogel treatment on wounded cells and in vivo lesion murine models, for achieving market preparation.

Knowledge Transfer on investigation of the anti-infective and antitumoral activity of novel cosmetic and pharmaceutical formulations based on natural extracts Call name: P 2 - SP 2.1 - Transfer de cunoaștere la agentul economic „Bridge Grant”
PN-III-P2-2.1-BG-2016-0369 2016 - 2018

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO); HOFIGAL EXPORT IMPORT SA (RO)

Affiliation: INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO)

Project website: https://icubresearch.wordpress.com/2018/10/19/bg-369/

Abstract:

The present project proposal aims the development of an innovative methodology for testing the antimicrobial, antiviral, anti-tumor and immunomodulatory activity, and to elucidate the mechanisms of action at the cellular and molecular level of natural bee and plant extracts with the purpose of introducing them în new cosmetic and pharmaceutical formulations, for expanding the marketed products range of the economic agent participating în the project - Hofigal S.A.

The addressed strategy is based on abundant scientific literature and original experimental results of all involved partners regarding the characterization and marketing of natural products / compounds with therapeutic or prophylactic value.

The proposed project falls into a major research direction aimed at the discovery of complementary therapeutic solutions based on natural compounds, for the treatment of different pathologies (i.e., infectious diseases and cancer), which are currently found în the top of causes of global morbidity and mortality.

The therapeutic alternatives that will be investigated în this project are based on natural compounds of vegetal and bee origin, with high therapeutic and preventive potential, due to the following properties: complex composition that allows simultaneous action on multiple biological targets; decreased risk for selecting resistance, both în case of infectious agents, such as viral, bacterial and fungal, and în the case of tumor cells; immunomodulatory effect; high biocompatibility; minimal or no side effects.

Development of functional model of microbial fuel cell for bioelectricity production with simultaneous municipal wastewater treatment Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-1008 2017 - 2018

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://sites.google.com/a/psg.unibuc.ro/fm-mfc

Abstract:

Microbial fuel cells (MFCs) are an emergent and a promising technology which converts organic matter into electricity with the help of exoelectrogenic bacteria present in the wastewater.

The scope of this project is to combine the results generated in current research, our research results on microbial fuel cells and interdisciplinary expertise of the research team and apply them to design a functional model of MFCs for treating municipal wastewater and generate bioelectricity in the laboratory environment. The minimization of the problems associated with the performance of MFC could be achieved by an effective electron transfer from microbes via anode which involves the high electrical conductivity of the anode materials and enhancing the surface area, porosity and biocompatibility of the anode. Furthermore, the availability of the anode materials is an essential requirement for MFC commercialization.

Our approach to improve the MFCs performance and for the development of a functional model of MFCs refers to the improvement of the electrode design , which is considered the biggest challenge in achieving a scalable and cost efficient MFCs technologies. Addressing this challenge, modification of the anode with advanced carbon based materials (such as CNTs, graphene and their composite with conducting polymers) will be used to improve planar and 3D configuration of MFCs anodes together with biofilm investigation and biocompatibility assay. Different MFC electrodes and reactor configurations will be tested for the improvements in power generation of MFCs and wastewater treatment in order to develop a functional model of MFCs.

Human lactoferrin-derived peptides with broad spectrum antiviral activity Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-1409 2017 - 2018

Role in this project:

Coordinating institution: INSTITUTUL DE BIOCHIMIE

Project partners: INSTITUTUL DE BIOCHIMIE (RO); INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO)

Affiliation: INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO)

Project website: http://peptivir.ucoz.ro/

Abstract:

Infections with Hepatitis B (HBV) and C viruses (HCV) are major public health threats with more than 400 million individuals being affected worldwide. Romania has a high prevalence of chronic infections, above the average of EU countries. Chronic patients are at high risk of developing end-stage liver diseases or hepatocarcinoma, and about 1 million people die annually due to such liver complications. Infections with the ubiquitous human pathogens Herpes simplex viruses (HSV) and the highly variable RNA viruses, Human adenovirus 5 (Ad5), Vesicular stomatitis virus (VSV), Enteric cytopathic human orphan 30 (ECHO subtype 30), Measles virus (MeV) also result in severe symptoms which require prolonged treatment.

A major drawback of the current antiviral therapies is the very high cost, which makes them unaffordable for many health care systems in developing countries.

Lactoferrin (Lf), an immunomodulatory glycoprotein was shown to interfere with the life-cycles of many viruses, regardless their origin or structure, both, in vitro and in vivo. In most cases, Lf was demonstrated to exert its antiviral effect at an early step of viral infection due to the crucial presence of cationic clusters in its structure. Recently, our group has rationally designed and characterized the anti-HBV activity of an Lf-derived peptide containing one of the cationic clusters. The peptide has better properties than the parental protein which could overcome the limitation for Lf use in clinical application. Thus, the major goal of this project is to take our initial observation further and investigate Lf-derived peptide(s) with increased solubility and efficient anti-viral action against a broad-range of human viruses, considering the pathogens listed above as models.

This project will prove the concept that the development of non-toxic, small Lf-derived molecule(s) with a broad-spectrum anti-viral activity may constitute a valuable, cheaper alternative to the current standard of care.

Magnetite nanoparticles functionalized with natural compounds to modulate Pseudomonas aeruginosa virulence and persistence Call name: Projects for Young Research Teams - RUTE -2014 call
PN-II-RU-TE-2014-4-2269 2015 - 2017

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://alina.amgtranscend.org/?page_id=44

Abstract:

Nowadays, about 70 percent of the bacteria that cause infections in hospitals but also in communities are resistant to at least one of the drugs most commonly used for treatment, causing infections that often fail to respond to standard therapy. Most resistant microorganisms in Romanian hospitals are the Gram negative non-fermenting strains of P. aeruginosa. The aim of this project is to investigate the effect of bioactive magnetite nanoparticles functionalized with plant-derived compounds on the virulence and persistence of some laboratory and clinically isolated, resistant P. aeruginosa strains. Our innovative approach is using subinhibitory concentrations of plant-derived compounds to modulate microbial behavior and virulence, rather than induce their killing, in order to limit the development and spread of resistant mutants, since strategies that interfere with bacterial population fitness are selecting resistant mutants. Knowing the intimate mechanisms of action of these bioactive nanoformulations with proved antimicrobial effect on the P. aeruginosa cells will significantly improve knowledge and offer further perspectives in developing alternative preventing and treatment approaches of this pathogen. This project is expected to have a great potential not only on the research field but also on the socio-economic sector, since it aims to develop an alternative method to solve or attenuate a global problem, as is the antimicrobial resistance.

Implementation of a complex genome profiling diagnostic algorithm for patients with congenital and developmental abnormalities Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-2240 2014 - 2017

Role in this project:

Coordinating institution: INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU"

Project partners: INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO); INSTITUTUL NATIONAL PENTRU SANATATEA MAMEI SI COPILULUI "ALESSANDRESCU-RUSESCU" BUCURESTI (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE "GR. TH. POPA" (RO); PERSONAL GENETICS S.R.L. (RO)

Affiliation: INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO)

Project website: http://www.virology.ro/ro/congen-proiect

Abstract:

The proposed project will set up a consortium in genomics, aiming at the implementation of new investigation methods in clinical practice that could contribute to the elucidation of the molecular mechanisms of congenital and developmental abnormalities with a final practical goal in prophylaxis and better clinical management of patients. The project corresponds to the direction 4 - HEALTH and its objectives refer to the national implementation of new prevention and intervention methods in accordance with European standards (4.1.6). The major objectives of the project are:

1) Investigation of genetic abnormalities in Romanian patients with congenital/developmental disorders aiming to identify possible new genomic variants;

2) Integration in the international endeavor of identifying and confirming new genomic variants for these rare disorders through active participation and inclusion of the Romanian data in the multi-center international consortia.

3) Implementation of a complex genome profiling diagnostic algorithm for patients with congenital and developmental abnormalities and use the integrated results for improving diagnostic yield, genetic counseling and clinical management.

The partnership consists of Stefan S. Nicolau IVN Bucharest, that will set-up the cells/DNA/RNA sample bank and perform PCR, MS-MLPA, sequencing analysis; Alfred Rusescu IOMC Bucharest and Grigore T. Popa UMF Iaşi that will recruit the patients, perform the phenotype/formal-genetic analysis and clinical management of the patients, and Personal Genetics Ltd. which will perform aCGH and next generation sequencing, will integrate the results of the analysis and will apply the resulting algorithm, firstly in their practice, and next will disseminate it to a clearly defined target market. The consortium represents a specialized and complementary team, capable to advancing the knowledge on clinical management, prevention, and eventually, therapy of congenital and developmental abnormalities, and to join international research effort in this direction.

Innovative nanostructured materials and coatings with antimicrobial activity for medical applications Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-1292 2014 - 2017

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU INGINERIE ELECTRICA ICPE - CA BUCURESTI

Project partners: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU INGINERIE ELECTRICA ICPE - CA BUCURESTI (RO); UNIVERSITATEA BUCURESTI (RO); MGM STAR CONSTRUCT S.R.L. (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.icpe-ca.ro/proiecte/proiecte-nationale/pn-2014/inmatco.pdf

Abstract:

The project aims to develop novel products and knowledge in antimicrobial nanostructured coatings for medical applications like critical surgical instruments (scalpels, knives, forceps and scissors) to reduce superficial and deep surgical site infections and to improve quality of life. Nanostructured materials of MeNPs/MeONPs type (MeNPs = Ag, Cu, MeONPs = CaO, MgO, ZnO, TiO2) with average MeNPs size of 10-20 nm and average MeONPs size of 30-50 nm) and high antimicrobial activity will be obtained by an innovative chemical synthesis of colloidal suspensions containing ecological NPs with 0.1-0.5 wt.% MeNPs and 1-3 wt.% MeONPs. Chemical deposition of MeNPs on MeONPs supports will be realized by a self-assembled nanolayers coating process, being a new and efficient approach in control of NPs size and stability. A higher content of MeNPs will be obtained in comparison with the existent solutions. The MeNPs and MeONPs mixtures will show synergistic effects of antimicrobial properties compared with those of MeNPs and MeONPs used alone. Ecological and efficient processing methods will be used to obtain and deposit the bioactive agents designed to inhibit the adhesion of microorganisms on stainless steel supports that show lack of antibacterial properties. The MeNPs/MeONPs composite powders will be deposited by different methods such as Thermal Evaporation, EBPVD (e-gun), Sputtering Deposition (Magnetron) and Arc-PVD (Plasmotron) on the metallic substrates with ensuring a good adhesion without changing the substrates original properties. Nanostructured coatings with homogenous and dense surface without any faults (pinholes and cracks) will be achieved at low temperatures to not affect the materials properties. Optimized coatings will be developed by a graded structure and adjusting stress level. Other original and innovative aspect is related by sputtering targets development from selected nanostructured materials by spark plasma sintering (SPS) process, where nanostructure features are maintained since grain growth and the development of equilibrium states are suppressed. The project addresses to the priority thematic of the PARTNERSHIPS PN-II-PT-PCCA-2013-4 program, Domain 7. “Materials, processes and innovative products“, Research field 7.1 Advanced materials, Thematic Area 7.1.6. “Advanced materials and biomaterials for health quality increasing“ with a direct impact in Domain 4. “Health” and European Research Area (ERA)-Nanomaterials. The project has inter-disciplinary, multi-disciplinary and trans-disciplinary nature being carried out by high skilled specialists with complementary expertise. Certified prototypes and end-products of critical stainless steel surgical instruments coated with antimicrobial MeNPs/MeONPs nanostructured materials with uniform and homogeneous mono/multilayer with thickness ranging 0.1-50 µm will be obtained. The coatings will have high resistance to mechanical wear, high resistance to heat stresses, high hardness, low friction coefficient and good adhesion to substrate. The novel products will be introduced on the market only after their certification granted by the Health Ministry from Romania. The coated products will be at a competitive cost with uncoated ones, contributing to the requests of the global and national socio-economic environment in the field of advanced materials and health with a major impact in reducing surgical site infections. Knowledge protection will be made by two patent applications registered to the national State Office for Inventions and Trademarks (OSIM). Results dissemination will be made on a large scale by publishing scientific papers in ISI ranked journals, attendance at national and international events, setting up and updating project web site, elaboration of dissemination materials.

Bioceramic composites with local applications in antibacterial therapy Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0005 2014 - 2017

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA

Project partners: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); SPITALUL CLINIC DE URGENTA (RO); UNIVERSITATEA BUCURESTI (RO); BIOSINTEX SRL (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.infim.ro/ro/projects/compozite-bioceramice-cu-aplicatii-locale-terapia-antibacteriana

Abstract:

In recent years, much attention has been paid to the synthesis and characterization of nanomaterials because of their interesting properties, which mainly come from the high surface/volume ratio. Hydroxyapatite incorporated with antimicrobial agents is able to prevent or cure infections by releasing directly the antimicrobial agents to local regions. HAp incorporated with Ag/Ag+, Cu2+/Zn2+, ampicillin and doxycycline have been studied to evaluate their antibacterial effect. Among those, Ag incorporation has gained more attention due to its broad-spectrum antibacterial properties. On the other hand in the latest years, the development and the use of antimicrobian agents in treating acute or chronic cutaneous lesions reached a development from the stage of clinical research to the appearance of some commercial products series.

Our project is based on a multidisciplinary approach involving physics, chemistry, microbiology, human toxicology and medicine. The project objectives are to highlight the major contributions that could be made by nanoengineered particles of hydroxyapatites for biomedical applications such inhibition and prevention of bacterial infections. The project will test methods to synthesis silver doped hydroxyapatite and collagen/silver doped hydroxyapatite able to inhibit the bacterial activity of different species of bacteria in the laboratory that will be implemented in real infected sites.

The decisive aim of this project is to develop specific nanoparticles/composites to prevent and remediate bacterial infections, without using antibiotics. For this reason, the new biomaterial based on nanoparticles of hydroxyapatite doped with silver and collagen/silver doped hydroxyapatites composites,will have antimicrobial properties due to their characteristics, but there is no risk to develop bacterial resistance.

Innovative antibacterial and self-cleaning photocatalytic textiles Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0419 2014 - 2017

Role in this project: Key expert

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU TEXTILE SI PIELARIE - INCDTP BUCURESTI

Project partners: INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU TEXTILE SI PIELARIE - INCDTP BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA (RO); UNIVERSITATEA BUCURESTI (RO); STOFE BUHUSI S.A. (RO); C & A COMPANY IMPEX SRL (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://cleantexproject.ro/

Abstract:

The negative impact of pollution on human health and environment pushed the R&D efforts to develop clean technologies and products according to the green chemistry principles, making regulation, control, clean-up and remediation unnecessary.

The project’ target is the development of new multifunctional textiles providing simultaneously photocatalytic, auto-sterilizing, self-cleaning and enhanced antimicrobial properties based on innovative graphene oxide/TiO2 nanocomposites able to decompose pollutants in safe, non-toxic compounds, using only solar light.

The present project aims at developing the 4th generation of green photocatalysts by:

• synthesis of graphene oxide/doped titanium oxide (GOT) with efficient absorption under UV and visible light

• formulation of GOT composites as highly adherent solution;

• development of photocatalytic textiles by deposition of photocatalytic compounds in one-step technology;

• evaluation of photocatalytic/self-cleaning/antimicrobial performances of synthesized photocatalysts and textiles against usual pollutants and pathogenic microorganisms,

• biocompatibility/cytotoxicity testing of cells cultures toward photocatalytic compounds.

The original contribution of the project consists in:

• optimal assembly and interfacial coupling of the TiO2 nanoparticles over the graphene oxide sheets;

• innovative adhesive graphene oxide/TiO2 (GOT) formulations, ensuring a high and stable dispersion and a strong adherence of the composites to the textile substrate, while preserving the genuine physical and mechanical properties of textile;

• homogeneously and firmly adherent photocatalytic coatings by one step deposition of GOT at room temperature, reducing the raw materials, utilities and manpower consumption;

• new investigations on graphene/TiO2 cytotoxicity and biocompatibiliy.

The indicators proposed to be achieved:

- eco-friendly products: minimum 2 types of composites nanopowders; min. 2 types of textile materials with high photocatalytic and antibacterial efficacy; self-cleaning and antibacterial work wear and protective equipment; clean technologies: one step synthesis of powder composites; one step deposition and fixation of synthesized compounds on textiles;

- innovations: 1 patent describing the innovative GOT synthesis and one-step deposition of nanocomposites; minimum 2 ISI rated scientific papers, minimum 2 presentations at national and international conferences.

The innovative approach for the production of high quality photocatalytic textiles is based on an efficient, environmentally clean and easily implementable at industrial-scale process, one step deposition and fixation of environmentally friendly nano composites. This finishing technology combined with a proper adhesive photocatalysts formulation will eliminate the post-treatment steps, and consequently high consumption of chemicals, water and energy and will allow the achievement of highly adherent, durable, uniform coating thin layers preserving the genuine physical properties and colour of the textiles. The easy application on conventionally existing production lines, ensure a wide spread of the finishing technology. The approval of the present project will contribute to concentration of human and material resources with the aim to achieve the above mentioned research tasks and the implementation of results in Romania, with special amelioration of the economic situation of SMEs active in textiles and chemical industry and significant improvements of human health and environment quality.

GENOMEWIDE STUDY OF BIPOLAR I DISORDER AND GUIDE FOR ASSESSING THE GENETIC RISK FOR BIPOLAR I DISORDER IN THE ROMANIAN POPULATION Call name: Joint Applied Research Projects - PCCA-2011 call, Type 2
PN-II-PT-PCCA-2011-3.2-1628 2012 - 2016

Role in this project: Key expert

Coordinating institution: SPITALUL CLINIC DE PSIHIATRIE PROF.DR.ALEXANDRU OBREGIA

Project partners: SPITALUL CLINIC DE PSIHIATRIE PROF.DR.ALEXANDRU OBREGIA (RO); INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO); UNIVERSITATEA BUCURESTI (RO); PERSONAL GENETICS S.R.L. (RO)

Affiliation: INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO)

Project website: http://www.bio.unibuc.ro/index.php?option=com_content&view=article&id=212%3

Abstract:

Medicine develops into three directions: prevention, prediction and personalization. Bipolar I disorder (BPI) is a chronic major psychiatric disease with a polygenic basis not yet elucidated. The objectives of the project are: 1) fine mapping of two haplotypes in NCAN and MAD1L1 genes found associated with BPI in a previous genomewide association study (GWAS) (Cichon et al, Am J Hum Genet, 2011) in which the project coordinator was involved; 2) a GWAS of the response to lithium therapy aiming at detecting the genotypes linked to positive response; 3) estimating morbid risks (MR) for relatives of BPI patients valid for the Romanian population; 4) determining the structure of Mad1 and Mad2 molecules and their neurobiological consequences with potential impact on drug development; 5) development of a guide for genetic counseling containing genotypes associated with BPI in the Romanian population, MR for relatives of patients, phenotypic variables that increase MR and genotypes predicting the positive response to lithium. The study will be conducted in an international consortium including Romanian partners. A Romanian sample of about 550-570 patients and 550-570 controls will be integrated in an international sample of several thousands of patients and controls that will be genotyped with high-throughput methods (Illumina, Sequenom) by Romanian researchers at the German partner and with next generation sequencing technology atone of the Romanian partners. Probands and their available first degree relatives will be investigated with DIGS and FIGS interviews and will provide psychiatric family history necessary for estimating MR. The response to lithium therapy in patients treated at least one year will be rated with the Alda scale. Our sample of lithium-patients will be integrated in the international lithium sample for genotyping. The biometric and molecular results of the study will be implemented in personalized medicine by a private health care company and disseminated among specialists.

Effects of P. aeruginosa quorum sensing molecules on Drosophila genome: a new tool to identify candidate genes involved in host-pathogen crosstalk Call name: Exploratory Research Projects - PCE-2011 call
PN-II-ID-PCE-2011-3-0576 2011 - 2016

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DIN BUCURESTI

Project partners: UNIVERSITATEA DIN BUCURESTI (RO)

Affiliation: UNIVERSITATEA DIN BUCURESTI (RO)

Project website: http://microbiologyresearch.wordpress.com

Abstract:

Pseudomonas aeruginosa, an emerging multi-drug resistant organism, one of the major contributors to morbidity and mortality in opportunistic and nosocomial infections, communicates intra-species and with the eukaryotic hosts by cross-kingdom inter-cellular signaling mechanisms such as quorum sensing (QS), relying on low-molecular weight excreted molecules, to control the production of virulence factors. The P. aeruginosa QS signaling molecules (QSSMs) have in vitro pleiotropic effects on eukaryotic cells. In the present project we propose an original, integrative in vivo approach for the investigation of the way in which such signaling molecules modulate the Drosophila melanogaster genome, in order to identify the most sensitive eukaryotic genes targeted by the QSSMs and the specific orthologous genes in the Homo sapiens genome. In order to accomplish our purpose, we will evaluate the phenotypic effects of QSSMs and viable P. aeruginosa cells (virulent versus quorum sensing defective strains) on D. melanogaster wild type and mutant strains, highlight the genes expressing a significant up- or down-regulation, identify by bioinformatics the orthologous human genes, assess the effects of P. aeruginosa QSSMs and viable P. aeruginosa cells on the respective genes profiles in the human eukaryotic cells and finally formulate the clinical contribution of our findings to future development of novel therapeutics for a multitude of infectious and immunological diseases.

Inovative, multidisciplinary research to investigate the probiotic effects of new lactic acid bacteria strains and consortia Call name: Joint Applied Research Projects - PCCA-2011 call, Type 1
PN-II-PT-PCCA-2011-3.1-0969 2012 - 2016

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL DE BIOLOGIE (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); UNIVERSITATEA DE STIINTE AGRONOMICE SI MEDICINA VETERINARA (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://genetmicroproiecte.blogspot.ro/search/label/PROLAB%2077

Abstract:

The emergence of bacterial resistance and multiresistance to antibiotics represent a major public health problem in Romania and also in the entire world. In Romania, there is an increasing trend of the incidence of infections with bacterial strains highly resistant to antibiotics, whose prognosis is often worsened by the producing of bacterial biofilms on the tissues and biomaterials used in medicine. In our country, the problem of development of some alternative strategies for the prevention and treatment of the infections with multiresistant and biofilm forming bacteria is less studied, despite their great application potential in biomedicine and their utility in the recovery of the balanced or eubiosis state of the normal microbiota, after the negative impact of the antibiotics on bacterial communities. One of the most viable alternatives is represented by probiotics products which by administration in adequate amounts confer a health benefit on human organism. For the best use of the probiotic microorganisms, the mechanisms by which they work should be better understood. The selection of an appropriate probiotic strain for its inclusion in a probiotic preparation should be made on the basis of its capacity to induce an improved host response without modification of the intestinal homeostasis.

The main goal of this project imply complex studies of probiotic strains (lactic acid bacteria newly isolated) and multispecific consortium in order to improve the experimental protocol for the optimal selection of human use probiotics, based on their anti-infective and immunomodulatory activities.

Role of S100A4 and MAP4K4 in pancreatic ductal adenocarcinoma progression Call name: Joint Applied Research Projects - PCCA-2011 call, Type 2
PN-II-PT-PCCA-2011-3.2-1490 2012 - 2016

Role in this project: Key expert

Coordinating institution: INSTITUTUL CLINIC FUNDENI

Project partners: INSTITUTUL CLINIC FUNDENI (RO); RNTECH S.R.L. (RO); INSTITUTUL DE BIOLOGIE SI PATOLOGIE CELULARA ,,NICOLAE SIMIONESCU'' (RO); INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO); INSTITUTUL ONCOLOGIC PROF.DR.ALEXANDRU TRESTIOREANU BUCURESTI (RO)

Affiliation: INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO)

Project website: http://www.icfundeni.ro/S100MAP/

Abstract:

Pancreatic cancer is the fourth leading cause of cancer death both in man and women. Annually, its incidence closely matches its mortality, highlighting the limited efficacy of existing treatment options. Most pancreatic cancers are pancreatic ductal adenocarcinomas and the 5-year survival rate for patients with localized disease after surgical resection is 20% and for those with metastatic disease, the survival rate is a dismal 2%. In the last years, investigators of the pathogenesis of this disease have turned their attention to the tumor microenvironment as a critical determinant of pancreatic tumor progression and clinical outcome. To address the question of the involvement of stromal cells in the pancreatic cancer progression, this project will have a specific interest on the interplay between stellate and pancreatic cancer cells analyzing the putative tumor markers in both cellular components.

Given the expertise of the consortium and the resources of the coordinating institute – consisting of a rich tumor biobank, an established Center for Cellular Therapies and a genomic platform – the present project will pursue two of the signaling pathways of the pancreatic cancer with the long-term goal to develop new remedies for this highly lethal disease. We will examine the role of the S100 calcium binding protein A4 (S1004) and the mitogen-activated protein 4 kinase 4 (MAP4K4) in pancreatic ductal adenocarcinoma progression. In doing so, we will consolidate the expertise and maintain the critical mass of scientists in areas of excellence and recruit strong external collaborators with significant expertise in these pathways and translational cancer research. Thus, the project is to set up a partnership in a priority research field – cancer genomics – to identify new molecular mechanisms involved in pancreatic ductal adenocarcinoma progression, which may result in rapid design and implementation of new therapeutic approaches targeting these pathways.

Novel nanostructured prosthetic tubular devices with antibacterial and antibiofilm properties induced by physico-chemical and morphological changes Call name: Joint Applied Research Projects - PCCA-2011 call, Type 2
PN-II-PT-PCCA-2011-3.2-0284 2012 - 2016

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); I.C.P.E. BISTRITA S.A. (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.antibiotube.ficai.eu

Abstract:

The project is intended to overcome the bacterial colonization of the prosthetic tubular devices, this being one of the most common reason of the tubular devices failures. The main objectives of the project include the surface modifications of the existing prosthetic tubular devices but, also bulk modifications will be tested, especially from the point of view of drug release properties. As surface modifications techniques, chemically modifications will be tested because it allows strong interactions between substrate and coating. Based on the existing expertise of P1-INCERPLAST, the only Romanian prosthetic tubular devices manufacturer, the PVC based devices will be used as substrate. The surface modifications will involve the partial substitution of Cl with HO followed by different chemical reaction: 1) alkylation/acylation when hydrophobic groups are intended to be attached on the surface of the tubular devices (in this way, the adhesion of bacteria is not favored); 2) thin polyurethane or silicon layer deposition starting from synthesized prepolymers, the withdrawal reaction being between prepolymer and hydroxyl groups of partially substituted PVC support; 3)coating of the tubular devices surfaces with antimicrobial nanoparticles, the stability of the coating being improved by proper functionalization of the substrate (if nanoAg are intended to be deposited on the PVC tubular devices, chloride will be partially substituted with SH; 4) the tubular prosthetic devices will be impregnated with antimicrobial/antibiofilm/anti-fouling agents, chemical bonding being prefered; and 5) new tubular devices will be obtained, the wall acting as a support for drug delivery. All these devices will be carefully selected and will be the subject of various scientific publications and patents. The ultimate goal of the project is the CE certification and finally their commercialization.

Synthesis and Antitumor Evaluation of Novel Pyrazole Compounds Call name: Projects for Young Research Teams - TE-2011 call
PN-II-RU-TE-2011-3-0228 2011 - 2014

Role in this project: Key expert

Coordinating institution: Universitatea de Medicina si Farmacie Carol Davila

Project partners: Universitatea de Medicina si Farmacie Carol Davila (RO)

Affiliation: Universitatea de Medicina si Farmacie Carol Davila (RO)

Project website: http://www.umfcaroldavila.ro/index.php/activitatea-stiintifica/programe-pnii/447-sinteza-si-evaluarea-antitumorala-a-unor-noi-compusi-pirazolici

Abstract:

The project focuses on improving the therapeutic solutions for a better treatment of the cancer patients by designing selective chemotherapeutic agents. The project main objective is the synthesis, the complex physical and chemical analysis followed by the anticancer screening, of a broad series of novel pyrazole based small-molecules for using them in antitumor therapy. In order to achieve this objective, we will use iterative cycles of design and chemical synthesis combined with biological evaluation, thereby progressively optimizing potency and selectivity while paying due attention to pharmaceutical and ADME (Absorption, Distribution, Metabolism, and Excretion) properties. Modern computerized prediction methods (in silico) will be performed to estimate the bio-pharmaceutical properties and the drug-likeness of the designed molecules, prior to their synthesis, greatly minimizing time and resources consuming steps of synthesis and biological screening of unfit molecules. State of the art equipment and methods will be used for the compounds synthesis and to determinate their physico-chemical and structural characteristics (HPLC-MS, IR, NMR, elemental analysis). The compounds anticancer quantitative evaluation will be performed in vitro on various human tumor cell lines, representing different cancer types, measuring also the substances effect on apoptosis and on the cell cycle. Structure-activity relationships studies will allow the optimization of the anticancer effects.

FILE DESCRIPTION	DOCUMENT
List of research grants as project coordinator
List of research grants as partner team leader
List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
R&D activities in enterprises
Peer-review activity for international programs/projects