BrainMap

Andreea Iosăgeanu

Researcher - INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Researcher | PhD student

My current interests are in wound healing process and tissue engineering, cancer biology, in vitro diagnostics and personalized medicine.

Curriculum Vitae (01/02/2024)

Expertise & keywords

Phytonutrients based on phytocomplecs enriched in phytoestrogens with potential for use in the management of metabolic diseases Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-1185 2022 - 2024

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); CENTRUL DE CERCETARE SI PRELUCRARE A PLANTELOR MEDICINALE PLANTAVOREL SA (RO)

Affiliation:

Project website: https://www.incdsb.ro/p/Phyto-ESM/

Abstract:

Nowadays, obesity and diabetes mellitus are two major nutritional disorders due to their frequent association with major cardiovascular risk factors, responsible for increasing mortality. Recent studies have shown that phytoestrogens have various beneficial effects on health by reducing the risk of cardiovascular disease, obesity, metabolic syndrome and type 2 diabetes and different form of cancers.

This project aim is technology for obtaining phytonutrients based on phytocomplexes concentrated in phytoestrogenic compounds from Trigonella foenum-graecum, Medicago sativa and Solidago virgaurea and evaluation of their effect on key enzyme linked to type-2 diabetes and obesity and also testing the biological effects using in vitro cell culture. The project technological development will foresee: a) Protocols for optimum extraction process, using modern extraction technologies: microwave assisted extraction, ultrasound-assisted extraction and accelerated solvent extraction of phytoestrogenic compounds; b) Achievement and validation of innovative laboratory technology based on selective separation processes (microfiltration/ultrafiltration for preconcentration and nanofiltration for phytoestrogens concentration); c) Analytical characterization and testing the biological effects of phytocomplexes enriched in phytoestrogens.

Involving a brand company in the domestic and foreign market in medicinal plants processing will lead to increasing the potential of the private company to get involved at performance level in scientific and technical and to develop new nutraceuticals, herbal drugs or food products for prevent of metabolic diseases.

The original results on the laboratory technology will be patented for transfer to the partner SME.

Technologies for preparation of some natural products with immunostimulatory properties Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-2118 2020 - 2022

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); HOFIGAL EXPORT IMPORT SA (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: https://unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte-de-cercetare/proiecte-cu-finantare-nationala/imunostim/ si https://unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte-de-cercetare/proiecte-cu-finantare-nationala/imunosti

Abstract:

The project scope proposal is the development of specific technologies for preparation of some vegetal extracts with immunostimulatory properties, which will be used in formulation of a food supplement for body invigoration. The novelties of this proposal are: a new own product, a combination of active principles with immunostimulatory properties; specific method of obtaining active principles with immunostimulatory properties; confirmation of non-aggressive effect of the nonconventional extraction techniques on the stability of extracted active principles.

The optimal plant extracts obtained by conventional and unconventional extraction methods will be selected. The optimal extracts contains the active principles from Hedera helix L. leaves and are obtained with the best possible extraction yield, without changing the structure of the extracted bioactive compounds. In all extraction stages, ecofriendly solvents (water, ethanol) will be used. Methods for analyzing and quantifying of selected bioactive compounds triterpene saponins, polyhydroxyalcohol-aldehyde (2-C-hydroxy-methyl-D-ribitol) , as well as corresponding standard compounds will be developed. Bioactive compounds of interest (from extracts and standard ones) will be analyzed qualitatively and quantitatively by spectroscopic methods ( 1H-NMR, 13C-NMR, IR, UV-Vis, HPLC / LC / MS / MS). The structure of the selected bioactive compounds will be highlighted by modeling methods and X-ray diffraction studies, to confirm their immunostimulatory properties. The bioactive compounds selected from the extracts and the standard ones will be tested by specific methods to highlight the immunostimulatory properties. The technology of obtaining the final product, dietary supplement, for the body's invigoration will be developed, with clinical impact.

Functional food with emergent properties to support hypocaloric diet Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-3805 2020 - 2022

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); SANTO RAPHAEL SRL (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://www.incdsb.ro/p/sinergem/

Abstract:

SINERGEM Project aims to develop a functional food as a protein bar used as adjuvant for hypocaloric diet, based on a combination with emergent properties of polyphenols and polysaccharides from forest fruits pomace (by-product resulting from obtaining the fruit juice at the economic agent partner) and chia seeds. The commercial opportunity is clearly established – the market for functional foods is increasing, driven by the requirement for healthier diets. The potential of forest fruits pomace as an affordable and sustainable source of polyphenol and polysaccharides is confirmed. Project objectives are (i) to select the synergistic composition of polyphenols and polysaccharides; (ii) to prepare a gastro-resistant nanoformulation of the synergic composition and (iii) to include the nanoformulated composition in a protein bar, verifying the preservation of emerging properties. The expected results of the project are: a mixture of polyphenols and polysaccharides (extracted from forest fruit pomace) and chia seeds, selected by a combination of high-throughput techniques and encapsulated into a gastro-resistant liposomal nanoformulation, which is included in a protein bar. Our proposed activities are intended to reach objectives related to: optimization of product composition (synergic composition); integration of product components into the demonstration model (nanoliposomes in a protein bar) and testing and validate its biological activity (as antioxidant, prebiotic and -amylase inhibitor) on laboratory level, on model systems. After the project implementation the economic agent partner will expand the range of functional foods that it can produce and sell.

Novel biotehnologies of plant by-products complex valorisation for prevention and control of pathogens in agro-ecosystems Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-3561 2020 - 2022

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); HOFIGAL EXPORT IMPORT SA (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://www.incdsb.ro/p/hydropest/

Abstract:

Nowadays, one of the major challenges for the R&D sector is to develop an efficient conversion of agro-food waste or by-products into innovative products, within circular bioeconomy. In this context, our project proposes valorisation of aromatic plants by-products in the form of hydrosols through novel biotechnologies in order to develop active products against various pathogens found in agro-ecosystems. Within the project, the multidisciplinary R&D and SME partnership will develop a new formula of biopesticides with functional properties and it will establish treatment protocols in experimental cultures, validating in vitro and in vivo their efficiency and safety in the labs of both partners. The project will start at TRL2 formulating the technological concept of plant hydrosols as waste products and will advance at TRL3 obtaining experimental variants of hydrosols with pesticide potential and it will end with TRL4 achieving validated formula in seedlings and plant model systems, which will be patented. High-throughput techniques (HPLC, GC, flow cytometry, fluorescence microscopy, mathematic modelling, PCR, microbial and cell culture experimental models, etc) will be used for the chemical and biological screening of hydrosols, to select functional models of biopesticides. The exploitation of aromatic plant by-products as biopesticides in crop pest management could open new opportunities in ecological agriculture, promoting natural, safer and autochthonous products. Furthermore, the partnership between R&D and industry sector will be strengthen in order to apply to future European calls regarding plant waste recycling and food security.

Biotechnology for a new biomimetic composite hydrogel based on extracellular matrix components and Si-bioceramics enriched with small molecules (flavonoids) for osteochondral regeneration Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-1714 2020 - 2022

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); CHEMI CERAMIC F SRL (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: https://www.incdsb.ro/p/biosicon/

Abstract:

Osteochondral defects are caused by severe trauma, accidental and sport-related injuries or diseases (osteoarthritis), which lead to pain and joint deformity. In 2012, it was estimated that 31 million people suffered from osteoarthritis in USA, among them 9.2% having osteochondral defects.

This project proposal aims the development of an innovative strategy for osteochondral regeneration by creating a novel biodegradable cross-linked composite hydrogel based on natural polypeptides (gelatin), natural polysaccharidic components (glycosaminoglycans) and silica-bioceramics (hydroxyapatite and tricalcium phosphate enriched with silica and akermanite) containing small molecules (flavonoids).

A key aspect is represented by Si-bioceramics use, as a better alternative to conventional bioceramics. The enrichment of the composite hydrogel with flavonoids (icariin and quercetin), as substitutes of growth factors will improve the healing of bone and cartilage. The physico-chemical and structural analysis of composite hydrogel will be performed by spectrometry, SEM-EDX, X-ray diffractometry, Raman spectroscopy, ion chromatography and mathematical modeling of the cristallization process. The validation of biomimetic system functionality and utility will be performed by analysis of genotoxicity, antimicrobial and antiinflamatory activity, the capacity of stem cells to proliferate and differentiate into osteoblasts and chondrocytes in the presence of composite hydrogel and angiogenesis in a co-culture of stem cells/HUVEC. In vitro biological activity will be demonstrated by experimental/demonstrative models in cell cultures using flow-cytometry, RT-PCR, ELISA, spectrometry and fluorescence microscopy. The original results regarding product and lab technology will be patented in view of transfer to the partner SME. The interdisciplinary public-private partnership will ease the development of new products that will bring benefits for people’s health and the access to European funds.

Functionalized mesoporous bioglass based 3D scaffolds for hard tissue regeneration Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-0598 2020 - 2022

Role in this project:

Coordinating institution: INSTITUTUL DE CHIMIE FIZICA - ILIE MURGULESCU

Project partners: INSTITUTUL DE CHIMIE FIZICA - ILIE MURGULESCU (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://www.icf.ro/pr_2019/BIOSCAFTIS/index.html

Abstract:

The project proposal “Functionalized mesoporous bioglass based 3D scaffolds for hard tissue regeneration“, aims to improve the well-being of people by treating them with new biomaterials. The objective of the project is to restore and improve the function of hard tissue by using functional bioactive scaffolds. These scaffolds will provide good mechanical properties in a suitable environment for tissue regeneration and repair. Sodium free mesoporous bioglasses (MBGs) doped with cerium will be used for scaffold manufacture using a combination of structure-directing agents and a polymer foam replication method. The BIOSCAFTIS project is based on our early results demonstrating that MBGs doped with cerium have good bioactivity and biocompatibility properties. In order to improve the growth and remodelling of bone tissue the surface of the scaffold will be functionalized with vitamin D3. The properties of the scaffolds e.g. bioactivity, mechanical strength, antimicrobial activity and drug delivery profile will be evaluated using adequate characterization techniques. The performance integration of 3D scaffolds to native tissues will be investigated using in vitro experimental models mimicking interaction with osteoblast cells. As a result of the research the project aims to disrupt the area of biomaterials by developing a new class of materials for medical applications.

Biorefining of the side streams resulted from cyprinids commercialization Call name: P 2 - SP 2.1 - Proiect de transfer la operatorul economic
PN-III-P2-2.1-PTE-2019-0181 2020 - 2022

Role in this project:

Coordinating institution: MEDICA FARMIMPEX S.R.L.

Project partners: MEDICA FARMIMPEX S.R.L. (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO)

Affiliation:

Project website: http://info.pro-natura.ro/colstim/

Abstract:

The aim of the project is to increase the competitivness of the coordinating company Medica Farmimpex and of the economic agents beneficiaries of the products obtained by the project, by assimilating some CDI results and by strengthening the partnership with research units in the fild of Bioeconomy-Biotechnology.

As result of this project it is expected to obtain high value-added products, trough an integrated extractive biotechnology, from the side streams of the commercialization of freshwater fish (cypridins).

Through the biotechnology that would be carried out at industrial level by the project coordinator, they will recover from the lateral flows of freshwater fish processing: collagen hydrolysate (nutraceuticals for human use) and gelatin like polypeptides (plant biostimulants). The remaining residue after the extraction of the peptides is processed into bone flour which is an essential nutritional supplement for feeding predatory fishes. Each of these products has a high use value associated with a significant market value.

The objectives of the project are: (i) to develope a pilot installation to obtain high valued products from the side streams of the fish commercialization; (ii) to validate the integrated technology in industrial environment; (iii) to demonstrate the functionality of the technology in the industrial environment by characterising and testing the obtained high added - value products.

The innovative character is given by the integrated concept of total capitalisation of the fish by-products. Another innovative aspect is the development of the quality assurance system, using NIR spectroscopic sensors coupled with a chemometric analysis.

The project parteners are INCDSB and INCD-ICHECHIM. INCDSB has the necessary expertise to transfer the technology for obtaining collagen hydrolysates. INCD-ICECHIM has the expertise to obtain and characterise the peptides as plants biostimulants.

Closing the bioeconomy value chains by manufacturing market demanded innovative bioproducts Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0569 2018 - 2021

Role in this project:

Coordinating institution: Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti

Project partners: Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO); INSTITUTUL DE CHIMIE MACROMOLECULARA "PETRU PONI" (RO); UNIVERSITATEA "DUNAREA DE JOS" (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO); UNIVERSITATEA AUREL VLAICU ARAD (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU STIINTE BIOLOGICE (RO)

Project website: http://icechim-rezultate.ro/proiect.php?id=51&lang=ro

Abstract:

Agriculture and food industry in Romania generates large amounts of co/by-products, which are not used and turn into wastes, with negative impacts. The approach of the project PRO-SPER is to develop integrated processes, flexible and interconnected, to transform a number of agro-food by-products in bio-products, with market demand. This approach facilitates the achievement of project goals, complex-coordination and linking of the research organizations that are members of the Consortium, INCDCP-ICMPP, UDJ, ICECHIM, INCDSB and UAV, for improving their institutional performance in the field of nano-and bio-technologies of their application into bioeconomy.

The overall objective of the project PRO-SPER is to increase the impact of research and development activities and innovation of the RDI institutions, by developing and harnessing innovative technological solutions for bio-nano-processing of several by-products from the bioeconomy value chains, for recovering and/or formation of value-added components and their use in order to obtain products with high added value.

Expected results through the implementation of the project (21 new jobs, 23 national patent applications and international patent applications 6 EPO/WIPO; 10 technologies/new products resulting from the project, at a level of technological maturity to enable taking over by the operators, 5 services research and technological research services 10 cheques, 10 experiments cheques services

80 internship of young researchers from and within partner institutions, 50 visits for developing new techniques for working jointly in the Consortium, 30 training internships for new employees, 28scientific papers, 1 joint program CDI, in line with the development plan of institutional partners) have a significant impact on the capacity-building of the partners in the Consortium.

FILE DESCRIPTION	DOCUMENT
List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
R&D activities in enterprises
Peer-review activity for international programs/projects