BrainMap

Mrs. Andra Ababei-Bobu

PhD Student - UNIVERSITATEA DE MEDICINA SI FARMACIE "GRIGORE T. POPA" DIN IAŞI

Researcher

Expertise & keywords

Development of new bioactive and biomimetic polymeric nanostructures for wound healing applications Call name:
PN-III-P4-ID-PCE- 2020-2687 2021 - 2024

Role in this project:

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE "GRIGORE T. POPA" DIN IAŞI

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE "GRIGORE T. POPA" DIN IAŞI ()

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE "GRIGORE T. POPA" DIN IAŞI ()

Project website: https://bionanowound.projects.umfiasi.ro

Abstract:

Wound care management represents a major health care problem in the entire world and biomedical research is constantly seeking for new options with respect to promoting the healing process and to reduce the therapy cost. The goal of the project is the design, development and evaluation of new biomimetic and bioactive polymeric nanostructures (BBPNs), for the treatment of acute and chronic wounds. The innovative designed nanostructures are based on chitosan/chitosan-hyaluronic acid nanoscaffolds, which have incorporated curcumin, propolis, honey, insulin, L-arginine or allantoin, as active substances, with important biological effects such as antimicrobial, antioxidant anti-inflammatory and healing. The innovative BBPNs will be: (i) characterized using Scanning Electron Microscopy, Transmission Electron Microscopy, FT-IR spectroscopy, X-Ray Diffraction, Thermal behavior; (ii) studied in terms of physico-chemical and mechanical parameters (porosity and swelling degree, contact angle measurements, tensile strength, entrapment efficiency, rheology, water vapor transmission); (iii) kinetic studies of delivery of the active substances; (iv) in vitro assays (antimicrobial and antioxidant effects, citotoxicity and hemocompatibility, cell migration and wound closure); (v) in vivo assays (wound model induced to diabetic and nondiabetic rats). The novelty of the project is supported by the original design of the BBPNs which assure antimicrobial, anti-inflammatory and healing effects.

Development of new pioglitazone-curcumin loaded chitosan nanoparticles with potential application in type 2 diabetes mellitus therapy Call name:
contract no. 23401 / 2018 2018 - 2021

Role in this project:

Coordinating institution: UNIVERSITATEA DE MEDICINA SI FARMACIE "GRIGORE T. POPA" DIN IAŞI

Project partners: UNIVERSITATEA DE MEDICINA SI FARMACIE "GRIGORE T. POPA" DIN IAŞI ()

Affiliation: UNIVERSITATEA DE MEDICINA SI FARMACIE "GRIGORE T. POPA" DIN IAŞI ()

Project website:

Abstract:

Diabetes mellitus is one of the most serious chronic diseases, which is considered an important public health problem. A relatively new class of drugs used in type 2 diabetes mellitus therapy is the thiazolidindione one from which pioglitazone are included. The alarming incidence of this malady coupled with the numerous side effects of the pioglitazone therapy led to an increased interest for developing new strategies with potential application in type 2 diabetes treatment. The main objective of proposed research is the development of new chitosan nanoparticles loaded with pioglitazone and curcumin and their antioxidant, antimicrobial and antidiabetic potential evaluation. The development of piogitazone and pioglitazone-curcumin loaded chitosan nanoparticles is based on ionic gelation process, using sodium tripolyphosphate (TPP) as cross-linking agent. The optimal conditions of formulation are set by varying different parameters such as concentration of pioglitazone, curcumin, chitosan and TPP solution, time and speed of stirring. The resulted nanosystems are characterised in terms of morphological, physic-chemical (yield, entrapment efficiency, drug loading, swelling degree, drug release) and spectral properties. The antioxidant potential of the obtained formulations is measured in vitro using the DPPH assay, while the antimicrobial effect evaluation is performed on different bacterial and fungal strains using the disk diffusion method. Based on the previous results, the best formulations of pioglitazone loaded chitosan nanoparticles and pioglitazone-curcumin loaded chitosan nanoparticles are tested in vivo for the antidiabetic effect on streptozotocin-induced diabetic model on rats, by determining the following parameters: clinical (weight, food and water intake), hematological (hemoleucogram) and biochemical (glycemia, glycated hemoglobine, renal and liver function and lipid profile).

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List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
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