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Romania
Citizenship:
Romania
Ph.D. degree award:
1993
Mrs.
Anca
Roseanu Constantinescu
PhD
Senior researcher, group leader
-
INSTITUTUL DE BIOCHIMIE
Researcher
>20
years
Personal public profile link.
Curriculum Vitae (26/02/2024)
Expertise & keywords
Cell biology
Inflammation
Infection
Nanotechnology
enzimology
Inflammation
Nanomedicine
Infection
Cell biology
Nanotechnology
Biocompatibility
Cell signalling
Cell proliferation
Intracellular trafficking
mammalian cells interactions with materials
Projects
Publications & Patents
Entrepreneurship
Reviewer section
Enhanced magnetic hyperthermia for malignant melanoma therapy
Call name:
P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-3292
2020
-
2022
Role in this project:
Key expert
Coordinating institution:
INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA
Project partners:
INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); INSTITUTUL DE BIOCHIMIE (RO); INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA MATERIALELOR BUCURESTI RA (RO)
Affiliation:
INSTITUTUL DE BIOCHIMIE (RO)
Project website:
http://ema-hypermat.inflpr.ro
Abstract:
The main objective of the project “Enhanced magnetic hyperthermia for malignant melanoma therapy” (EMA-HYPERMAT) is to increase the level of TRL maturity of a technology for treatment of the malignant melanoma by means of magnetic hyperthermia, implying drug delivery nanosystems developed by us. Nanoparticles could be efficient cytostatic delivery systems, capable of tumor targeting; thereby, the use of such nanosystems decrease adverse effects, increase therapy effectiveness, and increase the survival of skin cancer patients. The combination of chemotherapeutics and hyperthermia can be adjusted, depending on the type and site of the tumor, also on the drug dose and temperature. We propose to combine the advantages of hyperthermia with the controlled delivery of the antitumoral loaded into magnetic particle systems. In this respect, the novelty of our proposal is related on the use of hyperthermia enhancers based on the combination of iron oxide/silica core-shell particles and superparamagnetic iron oxide nanoparticles (SPIONs) embedded into the thermoplastic polymeric nanospheres. Both particle systems bring specific advantages. For such formulations, smaller concentrations of the iron oxide nanoparticles are necessary in order to obtain similar performances in hyperthermia tests which results in lower toxicity, reduced immunogenicity and side effects while magnetic polymeric nanospheres will provide improved efficiency in the case of hyperthermia and a better drug release due to the higher loading capacity of the polymer matrix.
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Smart responsive hybrid bioplatforms obtained by laser methods with tailored antibacterial and antitumor activity
Call name:
P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-2695
2020
-
2022
Role in this project:
Partner team leader
Coordinating institution:
INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA
Project partners:
INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); INSTITUTUL DE BIOCHIMIE (RO)
Affiliation:
INSTITUTUL DE BIOCHIMIE (RO)
Project website:
https://laurentiurusen.wixsite.com/529ped
Abstract:
The project scope is to develop an experimental prototype of a new generation of responsive hybrid smart platforms engineered by laser method, with tailored antibacterial and antitumor dual activity, addressing the current needs for research studies and medical applications (e.g. controlled drug delivery, development of next-generation precision medications testing platform, tunable interfaces for basic cell research). The platform envisaged by this project consists in a pH and Temperature responsive copolymeric matrix (i.e. Poly(N-isopropylacrylamide-co-butylacrylate (polyNIPAM-BA)) in which bioactive compounds such as antimicrobial peptides AMPs, Hydrophobines and Hydrophobines–AMPs conjugates) are embedded by the use of Matrix Assisted Pulsed Laser Evaporation (MAPLE). Based on the fact that tumor and bacteria cells exhibit more acidic medium and higher T compared to normal cells, this platform is designed for tumor and bacteria microenvironment-active /triggered drugs/bioactive compounds release. To achieve its goal, the project will follow 4 specific objectives:1-Designing the new smart hybrid platform based on innovative and complex hybrid coatings (i.e. pNIPAM-co-BA with/without single active bio-compound); 2. Developing the laboratory MAPLE technology for obtaining hybrid coating with enhanced sensitivity, antitumor and antibacterial potential by tailoring deposition parameters (laser, fluence, target system and composition) and by correlating it with the hybrid coating characteristics (thickness, porosity, composition: various ratio of pNIPAM-co-BA, Hydrophobines, AMPs).3. Optimizing the smart dual responsive engineered hybrid polymeric platforms with different compositions via laser methods and to demonstrate the bio-functionality using a tumor and bacteria microenvironment-active/triggered release of bioactive compounds; 4. To validate the efficacity of the hybrid platform at laboratory level (TRL 4) for various tumoral cells and bacterial lines.
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Integrated development project for advanced medical treatment technologies
Call name:
P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0728
2018
-
2021
Role in this project:
Partner team leader
Coordinating institution:
INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA
Project partners:
INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE MEDICO-MILITARA „CANTACUZINO” (RO); INSTITUTUL DE BIOCHIMIE (RO); UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA PITESTI (RO)
Affiliation:
INSTITUTUL DE BIOCHIMIE (RO)
Project website:
http://teramed.inflpr.ro/
Abstract:
In order to increase community’s quality of life, the aim of the project proposal entitled “Integrated development project for advanced medical treatment technologies” (TERAMED) is to develop novel technologies with respect to the treatment of osseous and cutaneous conditions and oncological disorders. Given our experience in healthcare research and the current requirements of multidisciplinary and interinstitutional collaboration towards the personalized treatment purpose, the TERAMED project aims genuine synthesis and processing of biomaterials, but also functional and therapeutic evaluation relevant for clinical trials. The main objectives of the “Medical devices functionalized by laser technologies and alternatives for enhanced osseous integration and regeneration” subproject are to design and produce inorganic, composite or hybrid coatings for superior osteoconductive and osteoinductive performances of titanium-based implants. Smart wound patches and polymeric gels functionalized with antimicrobial and wound healing biomolecules incorporated within micro- and nanoparticles constitute the purpose of the “Medical devices (patches and gels) based on composite biomaterials obtained by laser, plasma and radiation technologies and alternatives for enhanced healing of cutaneous injuries” subproject. The “Technologies based on magnetic triggered nanostructures for oncological therapy: early diagnosis and targeted treatment” subproject aims the development of multifunctional medical devices for specific and selective diagnosis and treatment of breast cancer and melanoma. The general impact of the TERAMED project ensues from the beneficial conjunction of the clinical potential of the proposed medical devices, the feasible technological transfer and the economic advantages of interinstitutional collaboration.
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Optimization and validation of an advanced material and technology default based on biopolymer-modified clay as carriers for controlled release of doxorubicin in gastrointestinal tract
Call name:
P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-1896
2017
-
2018
Role in this project:
Partner team leader
Coordinating institution:
Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti
Project partners:
Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO); INSTITUTUL DE BIOCHIMIE (RO)
Affiliation:
INSTITUTUL DE BIOCHIMIE (RO)
Project website:
http://www.icechim-pd.ro/ro/syst_heter/sisteme_heterogene_doxsaclay.html
Abstract:
The goal of the project DoxSaClay is to obtain, optimize and validate a new product and the associated technology reffering to the advanced material “biopolymer IPN hydrogel networks-modified clay-bioactive agent”. Preliminary results related to polymer-advanced modified clay nanocomposite materials are already demonstrated by the team’s publications. The consortium offers a high level of competencies in biomaterials domain, the Project Coordinating team having a great expertise in the synthesis of advanced materials while the competencies of Partner being related with in vitro testing on cell lines. The method will connect Salecan biopolymer with pH responsive (co)polymers to design new semi-interpenetrated networks (SIPN) with encapsulated amphiphilic nanoclays by free cross-linking radical co(polymerization) to render tunable and efficient advanced materials with enhanced mechanical strength, improved stability and adjustable responsive properties, for the controlled release of anti-cancer drug-doxorubicin in gastrointestinal tract. New delivery methods, particular delivery of doxorubicin, could make a dramatic impact to the medical community who administers the cancer treatment and the patient who endure the effects of the cancer treatment. The local, oral delivery of anti-cancer drugs to the colon maybe made possible by using designed technology, own modified clays and biopolymer semi-interpenetrated networks, by assembling them into novel structures and architectures that can exhibit properties appropriate for the application at hand. Validation of the results will be achieved by ISI publications in prestigious peer-reviewed scientific journals, conferences with international visibility and at least one patent request. Another priority of the present project is to increase the international visibility and competitiveness of Romanian research for successful integration in European Projects (Horizon 2020).
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Human lactoferrin-derived peptides with broad spectrum antiviral activity
Call name:
P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-1409
2017
-
2018
Role in this project:
Key expert
Coordinating institution:
INSTITUTUL DE BIOCHIMIE
Project partners:
INSTITUTUL DE BIOCHIMIE (RO); INSTITUTUL DE VIRUSOLOGIE "STEFAN S.NICOLAU" (RO)
Affiliation:
INSTITUTUL DE BIOCHIMIE (RO)
Project website:
http://peptivir.ucoz.ro/
Abstract:
Infections with Hepatitis B (HBV) and C viruses (HCV) are major public health threats with more than 400 million individuals being affected worldwide. Romania has a high prevalence of chronic infections, above the average of EU countries. Chronic patients are at high risk of developing end-stage liver diseases or hepatocarcinoma, and about 1 million people die annually due to such liver complications. Infections with the ubiquitous human pathogens Herpes simplex viruses (HSV) and the highly variable RNA viruses, Human adenovirus 5 (Ad5), Vesicular stomatitis virus (VSV), Enteric cytopathic human orphan 30 (ECHO subtype 30), Measles virus (MeV) also result in severe symptoms which require prolonged treatment.
A major drawback of the current antiviral therapies is the very high cost, which makes them unaffordable for many health care systems in developing countries.
Lactoferrin (Lf), an immunomodulatory glycoprotein was shown to interfere with the life-cycles of many viruses, regardless their origin or structure, both, in vitro and in vivo. In most cases, Lf was demonstrated to exert its antiviral effect at an early step of viral infection due to the crucial presence of cationic clusters in its structure. Recently, our group has rationally designed and characterized the anti-HBV activity of an Lf-derived peptide containing one of the cationic clusters. The peptide has better properties than the parental protein which could overcome the limitation for Lf use in clinical application. Thus, the major goal of this project is to take our initial observation further and investigate Lf-derived peptide(s) with increased solubility and efficient anti-viral action against a broad-range of human viruses, considering the pathogens listed above as models.
This project will prove the concept that the development of non-toxic, small Lf-derived molecule(s) with a broad-spectrum anti-viral activity may constitute a valuable, cheaper alternative to the current standard of care.
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Next generation of orthopaedic implants based on new Ti bioalloy functionalized with hybrid biomimetic coatings
Call name:
Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-1994
2014
-
2017
Role in this project:
Partner team leader
Coordinating institution:
INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA
Project partners:
INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); R&D CONSULTANTA SI SERVICII S.R.L. (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); UNIVERSITATEA BUCURESTI (RO); INSTITUTUL DE BIOCHIMIE (RO); TEHNOMED IMPEX CO S.A. (RO)
Affiliation:
INSTITUTUL DE BIOCHIMIE (RO)
Project website:
http://ppam.inflpr.ro/ORTHOBIOMIM.htm
Abstract:
The main objective of ORTHO-BIOMIM project is to obtain competitive and original products, namely advanced orthopaedic implants based on a new Ti-Ta-Nb alloy, which are functionalized by laser based technique (Matrix Assisted Pulsed Laser Evaporation) with complex hybrid biomimetic coatings (Lactofferin, Hydroxyapatite, Polyethilenglycol-Polycaprolactone synthetic copolymer functionalized with methyl ether group, Lf_HA_PEG-PCL-Me). The proposed approach relies on applied experimental research involving strongly interdisciplinary domains (i.e. material science, mechanics, laser physics, chemistry, microbiology, biomedicine), combined with industrial research activities, development.
The strategic key points for obtaining orthopaedic implant with enhanced characteristics within ORTHOBIOMIM project are:
• New and highly biocompatible Titanium (Ti)–based alloy containing non-toxic components (Ti-Ta-Nb) obtained by levitation technique with enhanced properties (low corrosion, mechanical resistance, elastic modulus etc.) working as implant body;
• Improved mechanical characteristics of the alloy obtained by its thermo-mechanical processing;
• Alloy biofunctionalization with complex hybrid coatings having multiple functionalities (biodegradable, bioresorbable, increased capacity osteogenic, osteoconductive and osteoinductive, anti-inflammatory ) by laser methods (MAPLE).To mimic the process of osseointegration, these coatings will be composed of natural biodegradable compounds, namely lactoferrin (Lf) and hydroxyapatite (HA), and a antifouling synthetic copolymer functionalized with methyl ether PEG-PCL-Me);
• Highly flexible methods for obtaining alloy processing and functionalization, allowing control defined composition, chemistry and surface topography when both alloy and the coating / film hybrid biomimetic functional.
The implications anticipated within ORTHOBIOMIM project development are related to major scientific benefits by increasing national competitiveness and visibility in research, development and innovation, but also to social and economic benefits . In the social context, in addition to the involvement of a large number of youth in the project and creating jobs, a very important benefit provided by this project is given by the envisaged faster osseointegration and long lifespan of the implant. This might be of crucial importance for those patients whose health status and life will be enhanced through the use of new medical implants by reducing risks of infection and thus reduce costs for medication or those necessary to replace a failed implant.
By the proposed objectives and approach, the project is aligned with the main points defined Partner Program, which promotes the applied inter and trans-disciplinary research in micro and nanostructured advanced materials for applications in medical field.
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FILE DESCRIPTION
DOCUMENT
List of research grants as project coordinator
List of research grants as partner team leader
List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
R&D activities in enterprises
Peer-review activity for international programs/projects
[T: 0.4714, O: 221]